FastQCFastQC Report
Wed 25 May 2016
SRR1780746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160917
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141438.789003026404917No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG5090.31631213607014796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGG150.00222141970.02
AACCGTG200.006941230552.58
TAACCGT200.006941230552.57
GCGGCTA200.006941230552.546
GCTCGGT200.006941230552.512
CGTTGAC305.8642577E-446.66666433
TGGTCGC757.4942363E-1042.00000449
TCGCCGT855.638867E-1141.1764752
ACAGGGC350.00125255340.0000048
GGTTAGA350.00125255340.0000041
CGGTGGT801.4097168E-939.37546
GATCTCG901.03682396E-1038.8888941
TCGGTGG852.5502231E-937.05882345
GCCGTAT852.5502231E-937.05882354
CGCCGTA951.8553692E-1036.84210653
GTCGCCG1051.2732926E-1136.66666451
CTCGGTG904.458343E-935.00000444
TCTCGGT904.458343E-935.00000443
GTAGATC1003.2196112E-1035.038
GCGGGCA400.002413275735.014