FastQCFastQC Report
Wed 25 May 2016
SRR1780746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160917
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT13070.8122199643294369No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10770.6692891366356568No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT3190.19823884362746014No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT1680.10440164805458715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT550.069.99969546
TGCCGAC353.6870915E-969.99969545
TTAGCAC150.002221458169.9996929
GACGTAT207.908117E-569.9996946
ATAGCGG150.002221458169.9996929
TCGGATG207.908117E-569.9996940
CAACTCG207.908117E-569.9996936
GACGACT150.002221458169.9996946
TTCGAGG252.829971E-669.9996929
TAATCCG252.829971E-669.9996943
TGACGTA600.064.1663845
CCGTATT600.064.1663847
TGCCGTA1050.063.33304645
CTTCGAG308.347739E-658.33307328
TCGTATG4400.056.47702440
GCCGACA252.3848929E-455.99975246
CTCGGAT252.3848929E-455.99975239
AACTCGA252.3848929E-455.99975237
CGACTTA252.3848929E-455.99975248
TATGCCG5000.055.29975543
Produced by FastQC (version 0.11.5)