FastQCFastQC Report
Wed 25 May 2016
SRR1780745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116732
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68255.846725833533221No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG2780.23815234897029092No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA150.002216812770.0244367
GCTTCTA150.00222058669.994431
ACTCGAT150.00222058669.994436
TCGATTG200.00693865752.4958238
GTGCTAA200.00693865752.4958233
CTCGATT200.00693865752.4958237
CGAAGCT200.00693865752.49582362
CTCGGTG506.141272E-848.99609844
GCCGTAT551.2955206E-744.56100554
TCGGTGG659.89894E-943.07349845
TGGTCGC503.4558761E-641.99665549
TCTCGGT602.574725E-740.83008643
CGCCGTA654.8070797E-737.70546353
GATCTCG654.8232687E-737.6893141
CGTATCA601.20433615E-535.01221556
ATCTCGG708.616553E-734.99721542
GTCGCCG601.2078815E-534.99721551
CCGTATC751.472652E-632.67806655
AGATCTC751.477596E-632.66406640
GTATCAT652.0829793E-532.3189757
Produced by FastQC (version 0.11.5)