FastQCFastQC Report
Wed 25 May 2016
SRR1780745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116732
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT17081.4631806188534422No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4930.42233492101565984No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG4500.385498406606586No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2600.22273241270602748No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2340.20045917143542472No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT1840.15762601514580407Illumina PCR Primer Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC150.002220046769.9987145
CGTCTTA150.002220046769.9987148
GCGTTTA150.002220046769.9987154
CTGTTCG150.002220046769.998719
CGTCTAC401.05610525E-861.2488848
AGTCTTC308.334111E-658.3322648
ACGCGAC252.3772885E-456.02297668
CTTTATA252.3823316E-455.998972
CGCGACG200.006925224352.5215469
AGGGGGG3600.052.52153870
CGTATGA407.42697E-752.4990441
TTCAACG200.00693697752.4990436
AAGCCGT200.00693697752.4990444
CGCTCAA200.00693697752.4990431
GTGCTAC200.00693697752.4990459
TCGAATG407.42697E-752.4990440
ATACAGG200.00693697752.499046
GTCTTCA750.051.3323949
GCCGACT352.076081E-549.99908446
CGAATGC352.076081E-549.99908441
Produced by FastQC (version 0.11.5)