FastQCFastQC Report
Wed 25 May 2016
SRR1780744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63002
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13622.16183613218628No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG750.1190438398780991No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTT200.00692172852.519
CTACACG200.00692172852.54
ATCCGTA305.836959E-446.66666412
GATTTAA350.001246757240.0000041
AGAACGG400.002402176135.070
CTCCTGG400.002402176135.01
GGTCTAG400.002402176135.01
TAGTAGG400.002402176135.02
CTATAGT500.00715956327.9999981
CGAGAAC901.9008707E-423.33333270
CATACAC1055.3882156E-420.0000023
TCCTGTC1600.00897180813.12551
TGCCGAC2153.160773E-413.02325670
CTGCCGA2550.001326226610.98039269
CGCTGCC3702.411043E-510.40540570
ACGCTGC3551.8320973E-49.85915669
CTGACGC4905.352569E-69.28571470
TCTGACG5006.9183843E-69.09999969
GCTGCCG3100.00669056469.03225869
GACGCTG3955.348073E-48.86075968
Produced by FastQC (version 0.11.5)