FastQCFastQC Report
Wed 25 May 2016
SRR1780744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63002
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2220.35236976603917336No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT820.1301545982667217TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT710.11269483508460049No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT207.871128E-569.999213
CCGTAGT150.002215229469.999235
ATAGGGC200.006922039752.4994058
AAGACCC200.006922039752.49940526
CCTACTT200.006922039752.49940531
CCAGCAT200.006922039752.49940530
AGTTCCC200.006922039752.4994055
CAAGACC200.006922039752.49940525
TTATAGA404.5456043E-543.74950440
GCCTGCG350.001246826839.99954616
TATTGAC400.002402310334.99960362
CTTCTAA450.004278154631.1107635
CTCTACA450.004278154631.110762
TAAGATC1055.388629E-419.99977330
TTAAATA900.005223131719.44422514
CGGAGTA1107.370984E-419.09069325
TTGAAAA1301.03770115E-418.8459455
TATGCCG950.007137552418.42084343
ACGGAGT1159.937712E-418.26066224
CTTGAAA1159.937712E-418.26066254
Produced by FastQC (version 0.11.5)