FastQCFastQC Report
Wed 25 May 2016
SRR1780743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37011
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29297.913863446002539No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG1710.46202480343681607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT150.002209062569.9905510
AGGAAAA150.002209062569.9905520
CGGGCAT150.002209062569.9905524
CTCTCGG150.002209062569.9905542
ATTGACC150.002209062569.990553
GACCTGC150.002209062569.990556
ACCTGCC308.2281695E-658.3254557
CTGCCCG252.3623934E-455.9924359
CCTGCCC252.3623934E-455.9924358
GCAAGAC200.006866044452.5639437
CAGCAAG200.006866044452.5639435
GTGCACT200.00690293452.49290548
CTATGGA200.00690293452.49290553
GAAGACC200.00690293452.49290546
AGTGCAC200.00690293452.49290547
AATTGAC200.00690293452.4929052
ACATGAA200.00690293452.4929055
TCATATT200.00690293452.49290558
CCTATGG200.00690293452.49290552
GGCTCAT200.00690293452.49290529
Produced by FastQC (version 0.11.5)