FastQCFastQC Report
Wed 25 May 2016
SRR1780743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37011
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3560.9618761989678744No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2660.7187052497906028No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT960.25938234578908975TruSeq Adapter, Index 2 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG640.17292156385939314No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC520.14049877063575694No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC460.12428737402393883No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT400.10807597741212072No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT150.00220804869.9986512
GGCCAAG150.00220804869.9986533
TCAGCAT150.00220804869.9986553
ATCTGAT150.00220804869.9986526
CTCGTAA252.3610416E-455.99892439
CTTAAGC200.006899771352.4989952
TGGAGGG200.006899771352.4989924
CGTATGA200.006899771352.4989941
TTTCGTG200.006899771352.4989929
CAGCATG200.006899771352.4989954
TGACGTC200.006899771352.4989945
AGTATAC200.006899771352.4989939
AAGCCGT200.006899771352.4989944
ACCAGCA200.006899771352.4989938
TTGGTCA200.006899771352.4989921
TAAATGT200.006899771352.4989925
GCCACCG200.006899771352.4989939
ATTTCGT200.006899771352.4989928
ATGCTTG305.806089E-446.66576454
CAATTTC305.806089E-446.66576426