FastQCFastQC Report
Wed 25 May 2016
SRR1780742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135722
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31302.306184701080149No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG2660.1959888595806133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTA200.006939910452.49806230
TGCTCGT200.006939910452.49806223
CGTGTGT200.006939910452.49806227
GCTCGTG200.006939910452.49806224
GTCGCCG451.6751674E-646.6649551
CTCGGTG503.459043E-641.9984544
ATCTCGG556.657345E-638.18041242
CGGTGGT556.657345E-638.18041246
GTAGATC601.2059261E-535.0116138
AAAGGGT400.00240802435.0116135
TCGCCGT501.7029297E-434.9987152
GCCGTAT501.7029297E-434.9987154
ACTGCCG400.00241238434.99870769
ACACTGC400.00241238434.99870767
GATCTCG652.085718E-532.31841341
TCTCGGT652.0909865E-532.30650343
CTGTGTA652.0909865E-532.30650334
CGCCGTA552.980305E-431.8170153
TCGGTGG552.980305E-431.8170145
TGGTCGC552.980305E-431.8170149