FastQCFastQC Report
Wed 25 May 2016
SRR1780742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135722
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5120.37724171468148127No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2210.16283284950118626No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT1920.14146564300555547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC150.00222066770.045
GCCGTCA352.9563125E-760.046
GGGTCGC252.3835037E-456.00000415
TGGGTCG252.3835037E-456.00000414
CGAATGC252.3835037E-456.00000441
CACGTAT252.3835037E-456.00000439
CGCGTAA252.3835037E-456.00000428
CGTATGA200.00693889852.541
CGGTTTA200.00693889852.562
CGTCTAC200.00693889852.548
ATCGTAT200.00693889852.539
ATATCGT200.00693889852.537
TCGTCTT200.00693889852.514
CGGTAAA200.00693889852.528
TCTGGCC200.00693889852.59
AATCACG352.0776637E-550.036
ACGTATG352.0776637E-550.040
CGTAAAA506.147275E-849.00000430
ACGCGAC305.8609917E-446.66666469
AGCGTCA305.8609917E-446.6666643
Produced by FastQC (version 0.11.5)