##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780742_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 135722 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.378619531100338 32.0 27.0 32.0 13.0 32.0 2 27.357429156658462 32.0 27.0 32.0 13.0 32.0 3 26.596874493449846 32.0 27.0 32.0 13.0 32.0 4 26.437136204889406 32.0 22.0 32.0 13.0 32.0 5 24.585874066105717 27.0 13.0 32.0 8.0 32.0 6 30.82641723523084 37.0 27.0 37.0 13.0 37.0 7 30.913389133670297 37.0 27.0 37.0 13.0 37.0 8 30.56659200424397 37.0 27.0 37.0 13.0 37.0 9 31.35595555621049 37.0 27.0 37.0 13.0 37.0 10-11 30.83907914708006 37.0 27.0 37.0 13.0 37.0 12-13 31.10598134421833 37.0 27.0 37.0 13.0 37.0 14-15 31.356073444246327 37.0 27.0 37.0 13.0 37.0 16-17 30.945266795361107 37.0 27.0 37.0 13.0 37.0 18-19 30.992945137855322 37.0 27.0 37.0 13.0 37.0 20-21 31.1897776336924 37.0 27.0 37.0 13.0 37.0 22-23 31.08337999734752 37.0 27.0 37.0 13.0 37.0 24-25 30.81543891189343 37.0 27.0 37.0 13.0 37.0 26-27 30.656669515627534 37.0 27.0 37.0 13.0 37.0 28-29 30.628980563210092 37.0 27.0 37.0 13.0 37.0 30-31 30.564458967595527 37.0 27.0 37.0 13.0 37.0 32-33 30.30300540811364 37.0 27.0 37.0 13.0 37.0 34-35 30.31825717275018 37.0 27.0 37.0 13.0 37.0 36-37 30.506697514036045 37.0 27.0 37.0 13.0 37.0 38-39 30.12222778915725 37.0 27.0 37.0 13.0 37.0 40-41 30.075127834838863 37.0 27.0 37.0 13.0 37.0 42-43 30.261891955615155 37.0 27.0 37.0 13.0 37.0 44-45 30.11059002961937 37.0 27.0 37.0 13.0 37.0 46-47 29.80300540811364 37.0 27.0 37.0 13.0 37.0 48-49 29.95724716700314 37.0 27.0 37.0 13.0 37.0 50-51 29.502066724628285 37.0 22.0 37.0 13.0 37.0 52-53 29.647393200807535 37.0 24.5 37.0 13.0 37.0 54-55 29.46478831729565 37.0 22.0 37.0 13.0 37.0 56-57 28.92169655619575 37.0 22.0 37.0 13.0 37.0 58-59 29.25921000279984 37.0 22.0 37.0 13.0 37.0 60-61 28.656477947569297 37.0 22.0 37.0 13.0 37.0 62-63 28.567461428508274 37.0 22.0 37.0 13.0 37.0 64-65 28.38476812896951 32.0 22.0 37.0 13.0 37.0 66-67 28.214029413064942 32.0 22.0 37.0 13.0 37.0 68-69 27.865412387085364 32.0 17.5 37.0 13.0 37.0 70-71 26.911812381190963 32.0 13.0 37.0 10.5 37.0 72-73 27.187073576870368 32.0 13.0 37.0 8.0 37.0 74-75 27.310926010521506 32.0 13.0 37.0 8.0 37.0 76 26.871988329084452 32.0 13.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.5584461344107545 11101 2 -0.6958454297585277 11101 3 -2.1668027988859215 11101 4 -0.7787871866629175 11101 5 -1.9198951220596676 11101 6 -0.042118719150195716 11101 7 -0.6097532812372108 11101 8 -2.3609152154488307 11101 9 -0.22994072861781945 11101 10-11 -2.0232869718615056 11101 12-13 -0.41310413215425257 11101 14-15 -0.057034151936186106 11101 16-17 -0.7164602828304609 11101 18-19 -1.556306867353019 11101 20-21 -1.4863164504150248 11101 22-23 -1.9106171884688017 11101 24-25 -1.0351133871390132 11101 26-27 -0.41400398411750317 11101 28-29 -1.527865960864066 11101 30-31 -0.8816157527812329 11101 32-33 -1.0216888981888914 11101 34-35 -2.3758487800473347 11101 36-37 -1.5574988112320902 11101 38-39 -0.7849629736941139 11101 40-41 -2.397258063921388 11101 42-43 -0.8778800137374887 11101 44-45 -3.859275003305598 11101 46-47 -3.24194352323018 11101 48-49 -2.3645573193323486 11101 50-51 -0.6807297901176064 11101 52-53 -2.13902787616826 11101 54-55 -1.1001288323246428 11101 56-57 -1.543100491321134 11101 58-59 -1.2315856866746238 11101 60-61 0.25139119212724736 11101 62-63 -0.13817176390009678 11101 64-65 0.0641638448481281 11101 66-67 -2.158756324160649 11101 68-69 -2.8835073097820256 11101 70-71 0.24386196238883429 11101 72-73 -2.369356713608802 11101 74-75 -3.155298273097408 11101 76 -3.2600794068595 11102 1 -1.2030580714279395 11102 2 -1.3252368201593185 11102 3 1.2795109072001765 11102 4 -3.759984613679247 11102 5 -1.539390421416421 11102 6 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21611 34-35 0.9205441957476346 21611 36-37 1.8264594232244882 21611 38-39 1.5815138495933425 21611 40-41 1.1649156427705876 21611 42-43 0.8216137372512762 21611 44-45 0.8057305337929606 21611 46-47 1.4291528222847703 21611 48-49 1.0063491828033904 21611 50-51 1.623652427885876 21611 52-53 1.3778998251448265 21611 54-55 1.4962166826255228 21611 56-57 1.4126818216040355 21611 58-59 1.2099583728096341 21611 60-61 2.3865754984079004 21611 62-63 0.5978668375870093 21611 64-65 1.3495617249303926 21611 66-67 1.9300745570290339 21611 68-69 1.1708353575174542 21611 70-71 0.7814351311912375 21611 72-73 1.8744338258627984 21611 74-75 0.5828760664058343 21611 76 2.2040876553142077 21612 1 0.4510494935838807 21612 2 1.158805733032171 21612 3 1.7579388031812648 21612 4 1.2824208236730001 21612 5 1.4468684438318036 21612 6 1.6983528570771966 21612 7 -0.6879186559142134 21612 8 1.1484517096157667 21612 9 0.5488060414080174 21612 10-11 -0.09063193310181816 21612 12-13 0.06985775415065731 21612 14-15 0.6574361323015445 21612 16-17 0.7073123269886601 21612 18-19 1.0298526933704935 21612 20-21 0.3308670121172739 21612 22-23 0.6049674368541993 21612 24-25 0.44631426143980235 21612 26-27 0.14914201071454158 21612 28-29 1.2531417910739187 21612 30-31 0.45704703791644263 21612 32-33 -0.08507714108294806 21612 34-35 1.0585827445004696 21612 36-37 0.15454899238548236 21612 38-39 0.9301533053756543 21612 40-41 0.28757720739643844 21612 42-43 0.03256895267077908 21612 44-45 0.5785479941104228 21612 46-47 -0.03562892374697668 21612 48-49 0.4145124481095124 21612 50-51 0.9092249902441516 21612 52-53 1.179770573444145 21612 54-55 0.6693317619906018 21612 56-57 1.1721772864566447 21612 58-59 0.1417185768912681 21612 60-61 1.2060908045303513 21612 62-63 1.4318379260223821 21612 64-65 0.9122743099644062 21612 66-67 0.21252920555511068 21612 68-69 0.5811244731637117 21612 70-71 1.6514039520529167 21612 72-73 0.059099358742617625 21612 74-75 1.0293754995124118 21612 76 0.5167293786702167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 7.0 17 36.0 18 108.0 19 303.0 20 780.0 21 1640.0 22 2688.0 23 4010.0 24 5488.0 25 7124.0 26 8476.0 27 10207.0 28 12151.0 29 13878.0 30 15628.0 31 16772.0 32 15629.0 33 12418.0 34 6456.0 35 1745.0 36 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.703578165527 29.172070266446593 11.228925960858287 15.89542560716812 2 28.02661342017816 32.86226891932715 15.529652743495848 23.581464916998844 3 25.79684944224223 31.217488690116564 15.315866256023341 27.669795611617864 4 17.35827647691605 27.64990200557021 21.303841676367867 33.68797984114587 5 18.99692017506373 37.04115766051193 20.00633648192629 23.955585682498047 6 27.18076053079479 40.320952542347904 19.773653303468144 12.72463362338916 7 27.819980990134024 34.18091792600889 22.357223380661516 15.641877703195572 8 25.435080532264482 38.54275651699798 18.537893635519666 17.484269315217873 9 25.927263081887975 23.433931123915062 20.062333298949323 30.576472495247636 10-11 25.378346915017463 28.78531115073459 25.020998806383638 20.81534312786431 12-13 25.547364581836668 26.06682213552316 25.179323378905604 23.206489903734568 14-15 23.565081563784794 26.507493258277954 22.638186882008814 27.289238295928442 16-17 24.138108886743932 30.154952512175893 23.300348514209507 22.40659008687067 18-19 23.522347150793536 29.490428965090405 25.030577209295473 21.95664667482059 20-21 22.399590330020114 29.450187519801947 24.986921506937126 23.163300643240813 22-23 23.422879120555255 30.34069642357171 24.486450243880874 21.74997421199216 24-25 23.670162796609233 29.97019632114293 25.078193211834527 21.281447670413307 26-27 23.276119320505313 30.37905665034612 25.681834090415084 20.66298993873348 28-29 23.052041350142574 29.375400643977628 26.4071353310885 21.165422674791294 30-31 23.157262639807843 28.759891543007026 26.661484505091288 21.42136131209384 32-33 21.948371076898137 29.86549365046033 27.40414902905647 20.781986243585067 34-35 22.67023769175226 30.84724657756296 26.28129558951386 20.20122014117092 36-37 22.769421204451763 30.638108184775444 25.35523111666169 21.2372394941111 38-39 22.0823094691625 29.792329031568787 26.485140307767125 21.640221191501592 40-41 21.72879894782215 28.95110099212709 27.050107391402058 22.26999266864871 42-43 22.768852163085164 28.049189326593993 27.42990189396591 21.75205661635494 44-45 21.512752404776027 29.347661905621518 27.3718957113753 21.767689978227153 46-47 22.545765346910354 27.778616926823336 26.718341874233253 22.957275852033053 48-49 21.74407981020026 27.289975096152432 27.794314849471714 23.171630244175596 50-51 20.679860594316196 28.489378789999925 28.326910749416072 22.503849866267803 52-53 20.234748875437944 28.237283514896532 29.39113391959151 22.136833690074013 54-55 19.696288709926982 28.22886657186434 28.857730196506065 23.217114521702612 56-57 20.36185592077632 28.478377800865033 27.81818047849569 23.341585799862948 58-59 19.367447686413204 28.717580312407897 28.62032124963159 23.2946507515473 60-61 20.358601990104734 29.98161676668766 27.15818790685337 22.501593336354226 62-63 19.121071012805587 31.513313980047453 25.912895477520227 23.452719529626737 64-65 19.474275609261884 32.30423489969974 24.64604785676129 23.575441634277084 66-67 19.294942878510458 32.27833877961531 24.57752938966029 23.84918895221394 68-69 19.161367806013807 32.44818414246874 24.437633085521032 23.95281496599642 70-71 20.45387562628942 31.133952254641912 24.514441497200117 23.897730621868554 72-73 23.114159826704586 28.40328023459719 24.221202163245458 24.261357775452762 74-75 23.39828251235066 28.587217205822217 23.910360554517894 24.10413972730923 76 23.707245906954125 28.204070204394405 24.27091469075583 23.817769197895636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 1.0 8 2.0 9 2.0 10 1.0 11 9.0 12 18.0 13 17.5 14 16.0 15 27.5 16 46.5 17 53.0 18 67.0 19 109.0 20 155.5 21 174.0 22 221.0 23 311.0 24 434.0 25 514.0 26 652.0 27 920.0 28 1203.0 29 1356.0 30 1553.0 31 1998.0 32 2625.5 33 3005.0 34 3245.5 35 3869.5 36 4659.0 37 5065.0 38 5566.0 39 6573.0 40 7433.0 41 8139.0 42 8491.0 43 8725.5 44 9089.0 45 9290.0 46 9362.0 47 9116.5 48 8443.0 49 7477.5 50 6940.0 51 6391.0 52 5343.0 53 4385.0 54 3926.0 55 3660.5 56 2965.5 57 2200.5 58 1865.0 59 1595.5 60 1132.5 61 866.0 62 793.0 63 689.5 64 547.0 65 460.0 66 381.0 67 350.0 68 338.5 69 314.0 70 285.5 71 270.0 72 247.5 73 196.0 74 175.0 75 183.0 76 170.0 77 155.5 78 152.5 79 151.0 80 122.0 81 90.0 82 79.5 83 72.0 84 69.0 85 53.0 86 34.5 87 29.0 88 22.5 89 13.5 90 8.5 91 7.0 92 8.0 93 7.0 94 7.0 95 7.5 96 7.0 97 10.5 98 27.5 99 267.0 100 493.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007368002239872681 2 7.368002239872681E-4 3 0.0 4 0.0 5 0.0 6 7.368002239872681E-4 7 0.0022104006719618043 8 0.0 9 0.0 10-11 0.0 12-13 0.0025788007839554383 14-15 0.0 16-17 0.0022104006719618043 18-19 0.0 20-21 0.0022104006719618043 22-23 0.0 24-25 3.6840011199363405E-4 26-27 0.0025788007839554383 28-29 0.0022104006719618043 30-31 0.0 32-33 0.0025788007839554383 34-35 0.0 36-37 3.6840011199363405E-4 38-39 0.0018420005599681702 40-41 0.0025788007839554383 42-43 0.0011052003359809021 44-45 0.0018420005599681702 46-47 0.0018420005599681702 48-49 0.0 50-51 0.0022104006719618043 52-53 0.0018420005599681702 54-55 7.368002239872681E-4 56-57 0.0036840011199363405 58-59 0.0014736004479745362 60-61 3.6840011199363405E-4 62-63 0.0 64-65 0.0033156010079427064 66-67 0.0018420005599681702 68-69 7.368002239872681E-4 70-71 0.0014736004479745362 72-73 0.0 74-75 3.6840011199363405E-4 76 0.0029472008959490724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 135722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.04504001487233 #Duplication Level Percentage of deduplicated Percentage of total 1 89.03306615261367 70.37622276680123 2 6.202873332734526 9.8061274158637 3 2.0124955122017183 4.772333648752074 4 0.9925276675233022 3.138175567809893 5 0.570690237986182 2.2555116348857385 6 0.33981344862780816 1.6116340586626112 7 0.23487092533917442 1.2995767184235592 8 0.13492584355748147 0.853217496243322 9 0.10394287156240527 0.7394551597719953 >10 0.3667984023529546 4.138894850569449 >50 0.004997253437990485 0.278085700971173 >100 0.001998901375196194 0.32627668892356376 >500 9.99450687598097E-4 0.40448829232170613 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 512 0.37724171468148127 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT 221 0.16283284950118626 No Hit ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT 192 0.14146564300555547 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 7.368002239872681E-4 0.0 2 0.0 0.0 0.0 7.368002239872681E-4 0.0 3 0.0 0.0 0.0 7.368002239872681E-4 0.0 4 0.0 0.0 0.0 0.0022104006719618043 0.0 5 0.0 0.0 0.0 0.0029472008959490724 0.0 6 0.0 0.0 0.0 0.0029472008959490724 0.0 7 0.0 0.0 0.0 0.0029472008959490724 0.0 8 0.0 0.0 0.0 0.0029472008959490724 0.0 9 0.0 0.0 0.0 0.0029472008959490724 0.0 10 0.0 0.0 0.0 0.0029472008959490724 0.0 11 0.0 0.0 0.0 0.0036840011199363405 0.0 12 0.0 0.0 0.0 0.005157601567910877 0.0 13 0.0 0.0 0.0 0.005157601567910877 0.0 14 0.0 0.0 0.0 0.005894401791898145 0.0 15 0.0 0.0 0.0 0.006631202015885413 0.0 16 0.0 0.0 0.0 0.006631202015885413 0.0 17 0.0 0.0 0.0 0.006631202015885413 0.0 18 0.0 0.0 0.0 0.007368002239872681 0.0 19 0.0 0.0 0.0 0.010315203135821753 0.0 20 0.0 0.0 0.0 0.013999204255758093 0.0 21 0.0 0.0 0.0 0.01915680582366897 0.0 22 0.0 0.0 0.0 0.02210400671961804 0.0 23 0.0 0.0 0.0 0.02210400671961804 0.0 24 0.0 0.0 0.0 0.02284080694360531 0.0 25 0.0 0.0 0.0 0.024314407391579848 0.0 26 0.0 0.0 0.0 0.024314407391579848 0.0 27 0.0 0.0 0.0 0.0346296105274016 0.0 28 0.0 0.0 0.0 0.04420801343923608 0.0 29 0.0 0.0 0.0 0.049365615007146965 0.0 30 0.0 0.0 0.0 0.06852242083081593 0.0 31 0.0 0.0 0.0 0.08841602687847216 0.0 32 0.0 0.0 0.0 0.10830963292612841 0.0 33 0.0 0.0 0.0 0.12894003919777192 0.0 34 0.0 0.0 0.0 0.15841204815726265 0.0 35 0.0 0.0 0.0 0.20998806383637142 0.0 36 0.0 0.0 0.0 0.31240329497060165 0.0 37 0.0 0.0 0.0 0.48113054626368607 0.0 38 0.0 0.0 0.0 0.7286954215234082 0.0 39 0.0 0.0 0.0 1.0595187220936915 0.0 40 0.0 0.0 0.0 1.448549240358969 0.0 41 0.0 0.0 0.0 1.9171541828148715 0.0 42 0.0 0.0 0.0 2.4410191420698193 0.0 43 0.0 0.0 0.0 2.979620105804512 0.0 44 0.0 0.0 0.0 3.486538659907753 0.0 45 0.0 0.0 0.0 4.013350820058649 0.0 46 0.0 0.0 0.0 4.530584577297711 0.0 47 0.0 0.0 0.0 5.034555930505003 0.0 48 0.0 0.0 0.0 5.554736888640014 0.0 49 0.0 0.0 0.0 6.064602643639204 0.0 50 0.0 0.0 0.0 6.564889995726559 0.0 51 0.0 0.0 0.0 7.0953861569973915 0.0 52 0.0 0.0 0.0 7.6737743328273975 0.0 53 0.0 0.0 0.0 8.187324088946523 0.0 54 0.0 0.0 0.0 8.683927439913942 0.0 55 0.0 0.0 0.0 9.103903567586684 0.0 56 0.0 0.0 0.0 9.546720502203033 0.0 57 0.0 0.0 0.0 9.949750224724069 0.0 58 0.0 0.0 0.0 10.358674349037003 0.0 59 0.0 0.0 0.0 10.779387276933733 0.0 60 0.0 0.0 0.0 11.233993015133876 0.0 61 0.0 0.0 0.0 11.66723154683839 0.0 62 0.0 0.0 0.0 12.093838876527018 0.0 63 0.0 0.0 0.0 12.518235805543686 0.0 64 0.0 0.0 0.0 12.944106335008327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGAC 15 0.002220667 70.0 45 GCCGTCA 35 2.9563125E-7 60.0 46 GGGTCGC 25 2.3835037E-4 56.000004 15 TGGGTCG 25 2.3835037E-4 56.000004 14 CGAATGC 25 2.3835037E-4 56.000004 41 CACGTAT 25 2.3835037E-4 56.000004 39 CGCGTAA 25 2.3835037E-4 56.000004 28 CGTATGA 20 0.006938898 52.5 41 CGGTTTA 20 0.006938898 52.5 62 CGTCTAC 20 0.006938898 52.5 48 ATCGTAT 20 0.006938898 52.5 39 ATATCGT 20 0.006938898 52.5 37 TCGTCTT 20 0.006938898 52.5 14 CGGTAAA 20 0.006938898 52.5 28 TCTGGCC 20 0.006938898 52.5 9 AATCACG 35 2.0776637E-5 50.0 36 ACGTATG 35 2.0776637E-5 50.0 40 CGTAAAA 50 6.147275E-8 49.000004 30 ACGCGAC 30 5.8609917E-4 46.666664 69 AGCGTCA 30 5.8609917E-4 46.666664 3 >>END_MODULE