FastQCFastQC Report
Wed 25 May 2016
SRR1780741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209334
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126526.0439297964019225No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG7080.33821548339018026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCGT150.002222626269.997841
TTTCCGC150.002222626269.9978462
GCCGTAT1300.061.93597854
TCGGTGG1600.061.2481245
TCTCGGT1650.055.14982243
CGGTGGT1650.055.14982246
TCGCCGT1400.055.01145652
GTCGCCG1400.054.98517251
GTAGATC1600.054.6858238
CGTATCA1550.054.20483856
CTCGGTG1850.052.97134444
GATCTCG1600.052.49838641
CCGTATC1550.051.94630455
AGATCTC1700.051.46900640
TGGTCGC1600.050.29893549
CATCGCG352.0819489E-549.99846322
CGCCGTA1700.049.42205453
GGTGGTC1750.047.99852447
ATCTCGG1700.047.35148642
GTATCAT1850.047.30714457
Produced by FastQC (version 0.11.5)