FastQCFastQC Report
Wed 25 May 2016
SRR1780741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209334
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT24471.1689453218301853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12980.6200617195486638No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG4640.2216553450466718No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4120.1968146598259241No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4020.19203760497578032No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT3610.1724516800901908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACT401.3460522E-1069.9909246
CCGACTT352.96679E-759.99221847
CGAATGC252.3852171E-456.00611541
CGTATGA950.055.2691941
TGCCGAC700.054.99286745
AATAACG200.006941134652.50573336
CATCGTT352.0831701E-549.99351532
ATCGTAT1000.049.0053539
ACGTATG506.1636456E-849.0053540
AGGGGGG9050.047.19875370
ATCTCGT14700.046.67176437
ATCTCGA604.9021764E-946.67176437
CGTCTAC451.6806425E-646.66061848
CCCGATA305.87208E-446.6606144
TCGTATG15550.046.5967340
TCTCGTA14900.046.28022438
CTCGTAT14700.046.19552239
CGTATGC15100.046.13086341
TATGCCG14500.046.0974743
AATCTCG14900.046.045336
Produced by FastQC (version 0.11.5)