FastQCFastQC Report
Wed 25 May 2016
SRR1780740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164335
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95735.825295889494021No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG4520.27504792040648673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAA150.002219931270.0124862
ACGTTAG200.00693659552.50935770
TCCGAAG200.00693659552.50935763
AGACGTT200.00693659552.50935768
CGCTCGC200.00693659552.50935730
TGCGATG200.00693659552.50935767
CCTGGTA200.00693659552.50935770
CACGTAA200.00693659552.50935761
TAAACGG200.006944952552.493384
TCGCCGT651.8371793E-1048.4554352
CTCGGTG651.8371793E-1048.4554344
GTAGATC703.8016879E-1044.99432838
TGGTCGC703.8016879E-1044.99432849
GCCGTAT757.4942363E-1041.99470554
CCGAAGC350.001251531840.00713364
GTATCCA350.001253407339.9949579
GTCGCCG801.4115358E-939.37003751
CGTATCA801.4115358E-939.37003756
CGGTGGT801.4115358E-939.37003746
TCGGTGG852.5538611E-937.0541545
Produced by FastQC (version 0.11.5)