FastQCFastQC Report
Wed 25 May 2016
SRR1780740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164335
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT25191.5328444944777437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8520.5184531597042626No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG4880.29695439194328654No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3270.1989837831259318No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2970.1807283901785986No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATG308.339046E-658.34424240
GCCGACT252.3863732E-455.99342746
GAGTTAA252.3863732E-455.99342748
ATTCCGG200.00693635752.5098151
CGAATGC200.00693635752.50981541
TCGAAAG200.00693635752.50981540
ATCGAAT200.00693635752.50981539
CCCGTGG200.00694471452.49383565
TTGGCAA352.0810425E-549.9941328
TGACGTC506.1636456E-848.99424745
GTCTTCA800.048.1193549
CCGTCTT6100.047.6173647
CGTCTTC6200.047.4137948
GCCGTCT6200.047.4137946
AACTCGT305.859215E-446.67539237
CTAATGG305.859215E-446.67539239
GCAACTG305.8680173E-446.66118631
CCGACTT305.8680173E-446.66118647
TGCCGTC7250.045.85668645
CGTATGC7800.045.77778641
Produced by FastQC (version 0.11.5)