FastQCFastQC Report
Wed 25 May 2016
SRR1780739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174891
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4478025.604519386360646No Hit
CTTATACACATCTGACGCTGCCGACGAGAACGGCTGTGTAGATCTCGGTG8900.5088883933421389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC150.002222281969.9957155
TCTACAG150.002222281969.995714
TGAGCGA252.386138E-455.9965731
CGCGTGC200.00693606452.51179540
GGCCGCC200.00694391652.4967853
AATGCAC200.00694391652.496783
CGCAAGG200.00694391652.4967814
TCCTTCA200.00694391652.4967850
TCCCACA200.00694391652.4967819
CCGCAAG200.00694391652.4967813
AGATCTC2000.049.0110140
GATCTCG2000.049.0110141
ATCTCGG2050.047.8019542
TAGATCT2150.047.21991339
GTAGATC2250.046.67715538
CAGTCGC305.867433E-446.6638156
GTCGCCG1800.046.66380351
TGGTCGC1900.046.0498149
GCCGTAT1600.045.9346854
CTCGGTG2000.045.4972144
Produced by FastQC (version 0.11.5)