FastQCFastQC Report
Wed 25 May 2016
SRR1780739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174891
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2384513.634206448587976No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG31161.7816811614091062No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT16570.9474472671549707No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15820.9045634137834423No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT15550.889125226569692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC9810.5609208020995934No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT6770.3870982497669977No Hit
CTTATACACATCTCCGCGCCCACGAGACTTATGAAAATCTCGTATGCCGT6340.3625115071673214No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT4260.24358028715028215RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC4020.22985745407139305No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3800.21727819041574467No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3500.20012464906713326No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT3010.17210719819773457No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT2790.15952793454208622No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2730.15609722627236394No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT2330.1332258378075487No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT2180.12464906713324297No Hit
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1900.10863909520787234No Hit
CATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1830.10463660222652968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT150.002220264470.01173439
AATCCGT150.002220264470.01173446
CGTATCC150.002220264470.01173441
AAGTTCT150.002220264470.01173459
TCTAATG150.002220264470.01173450
ATCTAGG207.914499E-569.991711
GTGACTT150.00222278669.991733
CTCAGCG150.00222278669.991712
AGGGGGG32400.064.0510570
GCCGACA650.059.24069646
CGTCTTA950.058.95724548
CGTATGA1200.058.3431141
GACGTCA308.342229E-658.3431146
CGTCTAC900.058.3431148
GCCGACT1500.058.3431146
CGTAAGA308.342229E-658.3431141
AACTCGT1200.058.3431137
CGACTTC1500.058.3431148
TAAGACG308.342229E-658.3431143
CCGACTT1400.057.50963647
Produced by FastQC (version 0.11.5)