FastQCFastQC Report
Wed 25 May 2016
SRR1780738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81245
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG150.002164492470.4275051
ATTCTCG150.00221821969.9938436
GCGCCAG200.00693131952.49538454
TATTGCG200.00693131952.49538450
CGTAGAT200.00693131952.49538437
GAACCTA200.00693131952.49538443
TCTATCG200.00693131952.49538470
GTATAGG350.001249430539.9964833
GCTAGGT500.007174669827.99753821
TAACTAT500.007174669827.99753849
TATTATA901.9086202E-423.331282
ATTATAC901.9086202E-423.331283
CCTTACC800.00264741721.8730758
CCCTATA850.003762871520.5864242
CTAGGAG1055.4099225E-419.9982413
CTCCTAG900.005054198719.5631981
ACCATAC950.007158105318.4194328
AGCAGTC1351.3930189E-418.14655370
ACGAGCA2653.362038E-815.84766270
GAGCAGT1554.0002435E-415.80506169
Produced by FastQC (version 0.11.5)