##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780738_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81245 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.305877284755987 34.0 31.0 34.0 12.0 34.0 2 31.764847067511848 34.0 34.0 34.0 23.0 34.0 3 31.9485629884916 34.0 34.0 34.0 27.0 34.0 4 32.190768662686935 34.0 34.0 34.0 27.0 34.0 5 32.12719551972429 34.0 34.0 34.0 27.0 34.0 6 35.21984122099821 38.0 37.0 38.0 27.0 38.0 7 34.51855498799926 38.0 35.0 38.0 25.0 38.0 8 33.6350421564404 37.0 34.0 38.0 21.0 38.0 9 34.17288448519909 37.0 34.0 38.0 24.0 38.0 10-11 34.61945350483107 38.0 35.5 38.0 25.5 38.0 12-13 34.86642870330482 38.0 36.0 38.0 26.5 38.0 14-15 34.59305803434057 38.0 36.0 38.0 25.5 38.0 16-17 34.44511046833651 38.0 35.0 38.0 24.0 38.0 18-19 34.43422364453197 38.0 35.0 38.0 24.5 38.0 20-21 34.515994830451106 38.0 35.0 38.0 24.5 38.0 22-23 34.4285679118715 38.0 35.5 38.0 24.5 38.0 24-25 34.85658194350422 38.0 36.0 38.0 26.5 38.0 26-27 34.83264816296388 38.0 36.0 38.0 26.0 38.0 28-29 35.045621269001174 38.0 36.0 38.0 27.0 38.0 30-31 34.93383592836482 38.0 36.0 38.0 27.0 38.0 32-33 34.518573450673884 38.0 36.0 38.0 25.0 38.0 34-35 34.583999015324025 38.0 35.5 38.0 25.5 38.0 36-37 34.577032432765094 38.0 35.5 38.0 25.0 38.0 38-39 35.01614868607299 38.0 36.0 38.0 27.0 38.0 40-41 34.55696350544649 38.0 35.5 38.0 24.5 38.0 42-43 34.83655609575974 38.0 36.0 38.0 26.5 38.0 44-45 33.90557572773709 37.0 34.0 38.0 22.5 38.0 46-47 34.64680288017724 37.5 36.0 38.0 26.0 38.0 48-49 34.45491414856299 38.0 35.0 38.0 24.5 38.0 50-51 34.294627361683794 37.0 34.5 38.0 24.5 38.0 52-53 34.302732475844664 37.0 34.5 38.0 24.0 38.0 54-55 34.0963505446489 37.0 34.0 38.0 24.0 38.0 56-57 34.13973167579543 37.0 34.5 38.0 24.0 38.0 58-59 34.03623607606622 37.0 34.5 38.0 24.0 38.0 60-61 34.05071081297311 37.0 34.0 38.0 23.5 38.0 62-63 34.09212874638439 37.0 34.0 38.0 24.0 38.0 64-65 34.16935196012062 37.0 34.0 38.0 24.0 38.0 66-67 34.11565634808296 37.0 34.0 38.0 24.0 38.0 68-69 33.576903194042714 37.0 33.5 38.0 22.5 38.0 70-71 33.51672718321127 37.0 34.0 38.0 22.0 38.0 72-73 33.65637885408333 37.0 34.0 38.0 22.5 38.0 74-75 33.191777955566494 37.0 32.5 38.0 21.5 38.0 76 32.34920302787864 36.0 31.0 38.0 20.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1822763501499693 1101 2 -0.7124918895699963 1101 3 0.08935223157272532 1101 4 -2.9946735569412084 1101 5 -0.8016832800348368 1101 6 -2.551149506158403 1101 7 -1.1140744475301432 1101 8 -0.5754150187770222 1101 9 -1.231301605554414 1101 10-11 -0.7974802859077812 1101 12-13 -0.9893921812932973 1101 14-15 -0.17907060050286816 1101 16-17 -1.7699788107166583 1101 18-19 -0.3851093371104142 1101 20-21 -1.4199515004047 1101 22-23 -0.28062344031582853 1101 24-25 -1.191267860440334 1101 26-27 -1.3813530437204946 1101 28-29 -0.8301507231443139 1101 30-31 -1.2496493302157248 1101 32-33 0.026793638240349082 1101 34-35 -1.095268733183424 1101 36-37 0.33784835022329673 1101 38-39 -0.8232038081498629 1101 40-41 -0.18183678665164393 1101 42-43 -0.5286518065040298 1101 44-45 -0.155962991153217 1101 46-47 0.1554519151321898 1101 48-49 -1.4421720713714095 1101 50-51 -0.9586678957380315 1101 52-53 -0.3902548826976542 1101 54-55 -1.8147376186218338 1101 56-57 -1.373029471146154 1101 58-59 -1.2854372217682837 1101 60-61 -0.093170728526907 1101 62-63 -2.0352863540344117 1101 64-65 -0.9403731736176795 1101 66-67 -1.675332554056034 1101 68-69 -0.2387365660620091 1101 70-71 0.08070551980501506 1101 72-73 -1.0021150799442538 1101 74-75 -1.536697431295238 1101 76 -1.3109533633165817 1102 1 -0.11793693017145301 1102 2 -0.7589115100497672 1102 3 -0.3865518142561335 1102 4 0.21581695505306442 1102 5 0.26668406690394164 1102 6 0.24883259194399443 1102 7 1.183365581112895 1102 8 1.1702527220035037 1102 9 0.6930986808759485 1102 10-11 -0.8528956099321263 1102 12-13 -4.322018389671381 1102 14-15 -1.2482793366288973 1102 16-17 -2.2374063080313746 1102 18-19 -1.0812067234333682 1102 20-21 -0.3582347084247459 1102 22-23 -1.5388225094171517 1102 24-25 -0.1252546130361054 1102 26-27 -6.133322252456626 1102 28-29 -0.6748249086079738 1102 30-31 0.7195325530638996 1102 32-33 -6.62178316090036 1102 34-35 -0.45746887639860034 1102 36-37 0.008417630567016943 1102 38-39 0.8383966214956828 1102 40-41 0.3777944342577655 1102 42-43 -0.09665216454197889 1102 44-45 -6.64193685438272 1102 46-47 -0.4074911568334372 1102 48-49 0.33629194581440913 1102 50-51 -6.67314157994856 1102 52-53 -1.5806594655834445 1102 54-55 0.4074249306083786 1102 56-57 -1.6825174768747644 1102 58-59 0.9801553310423117 1102 60-61 0.28626894207818054 1102 62-63 -4.937139200436128 1102 64-65 -0.8123925076670915 1102 66-67 0.11153640405352405 1102 68-69 -7.99423244862556 1102 70-71 -0.6919940863532403 1102 72-73 0.13026945281622915 1102 74-75 -7.80923592037508 1102 76 -2.802145629696817 1103 1 -0.2747093900450821 1103 2 -0.03335409847303694 1103 3 -0.16993477598174778 1103 4 0.7395151175920986 1103 5 0.30418860608615717 1103 6 -1.2288201061459176 1103 7 0.62493722756507 1103 8 1.4701147308639904 1103 9 0.9479610528710083 1103 10-11 0.6119674883320911 1103 12-13 -0.9568720464365796 1103 14-15 -0.8667774120156366 1103 16-17 -0.46896270283128416 1103 18-19 -0.5230253013983557 1103 20-21 -0.2911274384705038 1103 22-23 -0.25251268299548 1103 24-25 0.6923792020557613 1103 26-27 -0.2712044515996297 1103 28-29 0.7474933425358969 1103 30-31 0.145750869571998 1103 32-33 -5.6473260470939834 1103 34-35 -1.0994908097270937 1103 36-37 0.023182682495260565 1103 38-39 0.49506740368756397 1103 40-41 0.895159529762168 1103 42-43 0.9125278775816312 1103 44-45 -5.1957098226447584 1103 46-47 0.00832698537006138 1103 48-49 0.371697567137673 1103 50-51 0.6571075303717251 1103 52-53 0.14901589210412425 1103 54-55 0.6005398990156792 1103 56-57 1.3690258450803512 1103 58-59 0.8793564349714273 1103 60-61 0.8370206306539671 1103 62-63 0.4450236291084977 1103 64-65 0.8263902652285466 1103 66-67 0.18744605617060017 1103 68-69 -2.4748919010186228 1103 70-71 0.7187619598374866 1103 72-73 1.218946916684338 1103 74-75 -1.4953351287417007 1103 76 -0.22975838298318507 1104 1 -1.6757955960258535 1104 2 -0.2381530574149835 1104 3 0.17739884063269074 1104 4 0.00879593769089837 1104 5 -0.08153402630349404 1104 6 -0.0035585137520115495 1104 7 1.1989001531506673 1104 8 1.243843949539297 1104 9 -0.6202123151642596 1104 10-11 -0.2071752322288063 1104 12-13 -0.7753309087088454 1104 14-15 -0.1829655991936221 1104 16-17 -0.8263417329476113 1104 18-19 0.7288602884460076 1104 20-21 1.0399529248375146 1104 22-23 0.3985137683775477 1104 24-25 0.13411182010430878 1104 26-27 0.4711934344151416 1104 28-29 -0.4306351431495514 1104 30-31 -0.8814573951541931 1104 32-33 -0.9469887655302713 1104 34-35 -0.13775367688595708 1104 36-37 -0.1184556560610659 1104 38-39 -0.33567435017656777 1104 40-41 0.7925675076662344 1104 42-43 0.801702998418726 1104 44-45 -3.5461174818575927 1104 46-47 -0.2011859481828182 1104 48-49 0.94225448008185 1104 50-51 1.2779778230365153 1104 52-53 0.11216721732852619 1104 54-55 -1.1652220386270713 1104 56-57 0.5069129221505264 1104 58-59 0.35982331763993614 1104 60-61 0.15650196065875122 1104 62-63 0.3418738973402924 1104 64-65 -0.2560644540575865 1104 66-67 -0.47256350456664364 1104 68-69 -3.926809359987118 1104 70-71 -0.50115491486374 1104 72-73 0.8479438360023366 1104 74-75 -0.9088170961289634 1104 76 -0.9085574481555447 1105 1 -1.054731209927148 1105 2 -0.12128302800001478 1105 3 -0.6063194293313607 1105 4 -0.48109947575283485 1105 5 0.11619993164399745 1105 6 0.23541087289141416 1105 7 0.6669794646225 1105 8 0.13168576237293905 1105 9 -0.039375622714104 1105 10-11 0.6114869314055795 1105 12-13 -1.6856561075322531 1105 14-15 -0.46255760267983703 1105 16-17 0.5560392453724674 1105 18-19 0.27064043878918653 1105 20-21 -0.5083911213548831 1105 22-23 0.15563600391518406 1105 24-25 0.21769935687302677 1105 26-27 -0.034893829992746817 1105 28-29 -1.1110605707560453 1105 30-31 0.054919116448381544 1105 32-33 0.4496230658239071 1105 34-35 0.08066608561539823 1105 36-37 0.23873194607423898 1105 38-39 0.08603553107410988 1105 40-41 0.09758439403729824 1105 42-43 0.36145896312076076 1105 44-45 1.139673453974197 1105 46-47 0.7948103477099835 1105 48-49 0.2899639255552131 1105 50-51 -0.12679121498505452 1105 52-53 -0.7457421409893712 1105 54-55 -0.852034327547436 1105 56-57 -0.16756142145989372 1105 58-59 -0.032812045689396996 1105 60-61 -0.19207903945019922 1105 62-63 0.516048644044723 1105 64-65 0.5507908678729123 1105 66-67 0.3178003694937175 1105 68-69 0.968876444566753 1105 70-71 0.9228427973053428 1105 72-73 0.02074316054748948 1105 74-75 -1.2389320323965336 1105 76 -1.2037693448763207 1106 1 -0.23758411107771238 1106 2 -0.03526917175851807 1106 3 -0.6745490470475559 1106 4 -0.5438855700247629 1106 5 -0.06863243257721052 1106 6 -0.4013576536457606 1106 7 0.9587028346583821 1106 8 0.22506074690838318 1106 9 0.8651152841274197 1106 10-11 0.13743645509727287 1106 12-13 -0.11450368887581419 1106 14-15 0.1514007048792294 1106 16-17 0.17315232220038013 1106 18-19 -0.6244789475772592 1106 20-21 -0.33583761399375334 1106 22-23 -0.10815837935835049 1106 24-25 0.15466582971725984 1106 26-27 0.11481852927980185 1106 28-29 -1.2894132863319427 1106 30-31 -0.5672972636085234 1106 32-33 0.6077481431466865 1106 34-35 0.028132991467188617 1106 36-37 -0.10138001730568647 1106 38-39 -0.22730728718643434 1106 40-41 0.04370588358325733 1106 42-43 0.32222538647842924 1106 44-45 0.8105103957037514 1106 46-47 0.1795375529555585 1106 48-49 -0.1281667190074458 1106 50-51 0.45101096242431993 1106 52-53 -0.44351833794593887 1106 54-55 -0.22527136825014082 1106 56-57 0.7094489707212261 1106 58-59 0.3411982227752759 1106 60-61 0.6270939161355997 1106 62-63 0.612238177633742 1106 64-65 0.1044528745535942 1106 66-67 0.37919465379409445 1106 68-69 1.1456672400634744 1106 70-71 -0.11458142637398794 1106 72-73 -0.4332033939496043 1106 74-75 -1.1394330839862903 1106 76 -0.34329056224611065 1107 1 0.396671018271082 1107 2 0.5174579851167209 1107 3 0.17456526415417528 1107 4 -0.2791347098234134 1107 5 0.14004604327343628 1107 6 0.6938379625132782 1107 7 0.3928177830462971 1107 8 0.18335691104753948 1107 9 -1.7356875704666948 1107 10-11 -1.352304847311288 1107 12-13 -0.2816854143759997 1107 14-15 -0.7229302496256764 1107 16-17 0.6350963772532694 1107 18-19 0.8429608383281746 1107 20-21 0.7169532615000591 1107 22-23 -0.4015507586115703 1107 24-25 -0.7329345271070054 1107 26-27 0.18005046505142985 1107 28-29 -0.7139226529688756 1107 30-31 -0.030037801393675068 1107 32-33 1.1423951419997493 1107 34-35 -0.014128382306225262 1107 36-37 -0.650873454287975 1107 38-39 0.5658939362110473 1107 40-41 0.2573862709924626 1107 42-43 0.3062479428694047 1107 44-45 -0.16003567285748233 1107 46-47 0.17800830610963203 1107 48-49 0.23451965795189977 1107 50-51 1.2020463899762532 1107 52-53 0.9599957438687596 1107 54-55 0.05243030117765812 1107 56-57 1.131983060182172 1107 58-59 0.5861166629434962 1107 60-61 -0.28145393654695283 1107 62-63 -1.1396096623050838 1107 64-65 -0.2787190382793412 1107 66-67 0.8970358669965961 1107 68-69 1.0212884965946287 1107 70-71 0.13083083308822552 1107 72-73 0.018561611785074206 1107 74-75 -0.07867738116992484 1107 76 -0.5741112580534242 1108 1 -0.9856699693330633 1108 2 0.11886013915045268 1108 3 -0.16967371166374434 1108 4 -0.0921808386590186 1108 5 0.5506333222817688 1108 6 1.020702420956681 1108 7 1.5777395469154314 1108 8 1.4585042388264498 1108 9 0.15112405318703281 1108 10-11 0.13849986717763585 1108 12-13 0.8130615882657395 1108 14-15 1.251058776994931 1108 16-17 -0.2584212004748352 1108 18-19 0.8585064591369544 1108 20-21 0.7691784189883819 1108 22-23 -0.10313580230187114 1108 24-25 -0.38945759152480264 1108 26-27 -0.2662510996711873 1108 28-29 1.0236306898476286 1108 30-31 0.7069567119166322 1108 32-33 0.8300094480351063 1108 34-35 0.6517423756697909 1108 36-37 0.36203042703695587 1108 38-39 1.5018825564101874 1108 40-41 0.9214789350851262 1108 42-43 1.1528238420768844 1108 44-45 1.396507712633273 1108 46-47 1.2327735977170278 1108 48-49 0.5072792596588727 1108 50-51 0.6152754084684275 1108 52-53 0.8751044616090624 1108 54-55 0.8117271920535032 1108 56-57 1.0849397762967783 1108 58-59 -0.8018634224050061 1108 60-61 1.6189220033026643 1108 62-63 1.0722375232263133 1108 64-65 0.9925164829561837 1108 66-67 0.6815308883337181 1108 68-69 1.6663301547941884 1108 70-71 2.220802383586097 1108 72-73 1.3246985758167753 1108 74-75 1.5320216637396484 1108 76 0.7838444420075419 1109 1 -0.5910088555708235 1109 2 -0.7231857590752213 1109 3 0.054264512274478705 1109 4 -0.016652662007246022 1109 5 0.0681669072310811 1109 6 0.1359685699306965 1109 7 -1.262556607987463 1109 8 0.08262755957898094 1109 9 0.5507307224566276 1109 10-11 0.713977431419238 1109 12-13 0.2495936259213778 1109 14-15 0.08645275845633194 1109 16-17 -0.4532283671921178 1109 18-19 -0.13565142266137542 1109 20-21 0.6655748153847796 1109 22-23 0.6507288167312879 1109 24-25 0.7518377969615955 1109 26-27 0.06586753581823501 1109 28-29 -0.1663176854940147 1109 30-31 0.07168420023963051 1109 32-33 0.7520301846625799 1109 34-35 0.21596229382229382 1109 36-37 0.6790665642978126 1109 38-39 0.5937585415445952 1109 40-41 0.5877768541920361 1109 42-43 0.36014673479304093 1109 44-45 -0.18151493280510067 1109 46-47 -0.3156697091532479 1109 48-49 -0.1893138021027312 1109 50-51 0.6905288320364562 1109 52-53 0.7582765582642992 1109 54-55 -0.0998746184247139 1109 56-57 0.3650939272374458 1109 58-59 0.22816419751025308 1109 60-61 0.22486100811807574 1109 62-63 -0.29309731401863814 1109 64-65 -0.15124445293957933 1109 66-67 0.24712457190493353 1109 68-69 0.9977112951640876 1109 70-71 0.3997967872996 1109 72-73 -0.11856491239053213 1109 74-75 1.4447730989629903 1109 76 -0.6595636530859466 1110 1 0.9424472234900136 1110 2 0.2403682018349862 1110 3 0.1467014870643979 1110 4 0.7993703764216136 1110 5 0.5911738628223091 1110 6 0.44859249590557937 1110 7 0.8994520156867196 1110 8 1.9356788924716923 1110 9 -0.9342722675034025 1110 10-11 -0.7725834850821869 1110 12-13 -0.07513963817078206 1110 14-15 -0.25212087324354115 1110 16-17 0.12591902210067474 1110 18-19 0.2908821121008458 1110 20-21 -0.314117061365927 1110 22-23 0.11171770666928182 1110 24-25 0.6571583521499633 1110 26-27 -0.7038504637411407 1110 28-29 0.4545868560979116 1110 30-31 0.39654335738563873 1110 32-33 1.3708338859183655 1110 34-35 0.8654602952700614 1110 36-37 0.05025436526089777 1110 38-39 0.8313738123720285 1110 40-41 0.8393190441017069 1110 42-43 0.7707547982431393 1110 44-45 1.651220408522434 1110 46-47 0.3491715082325868 1110 48-49 0.5289690166427476 1110 50-51 0.3768584200514411 1110 52-53 0.3761832715114011 1110 54-55 0.18953777574643738 1110 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2116 48-49 -0.024822559868098892 2116 50-51 -0.42703379551742415 2116 52-53 0.050074983884975666 2116 54-55 -0.47825269373476686 2116 56-57 -0.6932836995314844 2116 58-59 -1.3824011885715208 2116 60-61 -1.6043457469111857 2116 62-63 -0.5190162626382531 2116 64-65 0.1731629121056102 2116 66-67 -1.0873637548124222 2116 68-69 -0.24987218955187274 2116 70-71 -3.2319060990625097 2116 72-73 -0.07625627124586032 2116 74-75 -1.1736866108309059 2116 76 -0.9767270790430231 2117 1 0.9226311889680971 2117 2 0.2858485561388733 2117 3 0.3785888365988015 2117 4 0.2224909760128 2117 5 -0.5178994962510544 2117 6 -0.12891147865721564 2117 7 -1.4367392175633356 2117 8 -2.1843529040306926 2117 9 1.6636723157352975 2117 10-11 0.9024960061737772 2117 12-13 0.9887372639414167 2117 14-15 -0.11091033497417868 2117 16-17 0.5861733941197542 2117 18-19 0.012946613577113908 2117 20-21 0.5428270676370275 2117 22-23 0.810474236308167 2117 24-25 0.660735458668249 2117 26-27 1.270911062490974 2117 28-29 0.7736306898476286 2117 30-31 -0.029047149087233493 2117 32-33 1.330492073517732 2117 34-35 -0.04951245058503417 2117 36-37 -0.6987803837738511 2117 38-39 0.4719597764874024 2117 40-41 -0.8019766633704677 2117 42-43 -0.6541259648729252 2117 44-45 0.8921640832896429 2117 46-47 0.3727349876784203 2117 48-49 -0.08104120366159151 2117 50-51 0.9613178795109008 2117 52-53 1.2530002145048158 2117 54-55 0.32620595653227014 2117 56-57 -0.15444246308546639 2117 58-59 0.24253812199653169 2117 60-61 1.1951768295574965 2117 62-63 0.32513327612205956 2117 64-65 0.2637520041917085 2117 66-67 -0.22049613869330642 2117 68-69 0.11131084977488115 2117 70-71 0.9785243913081061 2117 72-73 1.6287526298708244 2117 74-75 0.3413845981025858 2117 76 -0.09260343444033481 2118 1 0.7776939982562574 2118 2 -0.00202734793421655 2118 3 0.23736310382377468 2118 4 0.6134312120234569 2118 5 0.2159223537679793 2118 6 0.580738311978493 2118 7 0.6613765037918213 2118 8 -2.6761686634520885 2118 9 -0.2620033599403797 2118 10-11 -0.2746547361178102 2118 12-13 0.9289732745999615 2118 14-15 -0.5613290635619208 2118 16-17 0.16858679343456373 2118 18-19 0.81757166115991 2118 20-21 -1.627852414662172 2118 22-23 -0.0872703392533083 2118 24-25 0.20127599920878936 2118 26-27 0.8410956837319361 2118 28-29 -0.4541005625315151 2118 30-31 -0.34913660474006747 2118 32-33 -0.007583678290416174 2118 34-35 0.5996596087982979 2118 36-37 0.3515622123433886 2118 38-39 -0.0012986932499359227 2118 40-41 -0.7783280147218221 2118 42-43 0.0035634146474379236 2118 44-45 0.33035403380353756 2118 46-47 -0.28895134654312216 2118 48-49 -0.837464880859919 2118 50-51 -0.13722608699081462 2118 52-53 -1.6279000519588394 2118 54-55 0.7884252180473013 2118 56-57 1.2000493526739646 2118 58-59 0.8825267021259577 2118 60-61 -0.43691300675769185 2118 62-63 0.682004231584564 2118 64-65 0.024600881237766714 2118 66-67 -1.8405475281108963 2118 68-69 1.0853306442172155 2118 70-71 -0.6961044628982194 2118 72-73 -0.5484456952148165 2118 74-75 0.8727416593892201 2118 76 -0.6363796049770691 2119 1 1.1231194923975742 2119 2 0.45885559679297216 2119 3 0.415609050089607 2119 4 0.5598745781022814 2119 5 0.7046192409735639 2119 6 1.2217017396030627 2119 7 -1.087942942296472 2119 8 1.6415612454128663 2119 9 1.200181514009202 2119 10-11 1.1601896241615037 2119 12-13 0.9311628828376257 2119 14-15 0.0966186225547716 2119 16-17 0.04398624899143755 2119 18-19 0.22575606168067708 2119 20-21 -0.5221002546432416 2119 22-23 1.1201210680138232 2119 24-25 0.6004356630743359 2119 26-27 0.6133764270151687 2119 28-29 1.1646708897113527 2119 30-31 0.4028458783940323 2119 32-33 0.9149515329771916 2119 34-35 0.8570569339255272 2119 36-37 0.12378377702560073 2119 38-39 0.4469200308594239 2119 40-41 0.8918400525050743 2119 42-43 0.29806572261288267 2119 44-45 -0.04625858307419861 2119 46-47 0.3659782309216624 2119 48-49 0.20123792420577047 2119 50-51 1.4083710250934587 2119 52-53 -0.6175142074801983 2119 54-55 -0.5205462578670037 2119 56-57 1.3767862446138324 2119 58-59 1.7102873838634451 2119 60-61 1.3584178016220037 2119 62-63 1.3707839688315815 2119 64-65 1.3704406255862125 2119 66-67 0.7656780607161906 2119 68-69 0.6505454625389078 2119 70-71 0.5329759016419686 2119 72-73 -0.6656489145307134 2119 74-75 0.9262982933103885 2119 76 0.7781664951531226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 8.0 13 93.0 14 225.0 15 413.0 16 576.0 17 666.0 18 618.0 19 658.0 20 610.0 21 495.0 22 451.0 23 425.0 24 551.0 25 641.0 26 762.0 27 946.0 28 1155.0 29 1474.0 30 1779.0 31 2530.0 32 3181.0 33 4332.0 34 6449.0 35 10111.0 36 18228.0 37 23867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.67756638921919 22.127675386444707 12.518579072532699 28.67617915180341 2 18.87254600283094 19.597513693150347 35.848359899070715 25.681580404947997 3 17.331528094036557 24.9763062342298 28.44236568404209 29.24979998769155 4 12.867253369438119 17.542002584774448 36.51301618561143 33.07772786017601 5 14.090713274663056 35.884054403347896 34.73567604160256 15.289556280386485 6 28.949473813773157 37.658932857406604 17.027509385192936 16.3640839436273 7 27.82448150655425 30.362483845159705 21.730568034955997 20.082466613330052 8 23.77377069358114 35.67357991261001 18.907009662133053 21.645639731675796 9 23.71345928980245 17.175210782201983 21.058526678564835 38.05280324943073 10-11 24.69321188996246 25.591728721767492 26.751800110776045 22.963259277494 12-13 24.03040187088436 23.885162163825466 25.992368761154534 26.09206720413564 14-15 21.33977475536956 25.6865037848483 23.472213674687673 29.501507785094468 16-17 22.302910948366055 27.54754138716229 26.141301003138658 24.008246661333004 18-19 21.396393624223027 27.237983875930826 26.646562865407102 24.71905963443904 20-21 21.20438180811127 28.036802264754755 26.385008308203584 24.373807618930396 22-23 22.657394301187768 26.842882638931627 26.81580404947997 23.68391901040064 24-25 22.035817588774695 27.901409317496462 26.554249492276448 23.508523601452396 26-27 20.805588036186844 28.326666256385007 27.197365991753337 23.670379715674812 28-29 21.59640593267278 26.90011693027263 28.337743861160686 23.165733275893903 30-31 21.81795802818635 27.313680841897963 27.226906271155148 23.64145485876054 32-33 20.545879746445937 27.92294910456028 27.44907378915625 24.08209735983753 34-35 21.968121115145546 27.45645885900671 27.096436703797156 23.47898332205059 36-37 22.25921595175088 26.84165179395655 26.91119453504831 23.98793771924426 38-39 21.612406917348757 27.30691119453505 27.726013908548218 23.35466797956797 40-41 21.816727183211274 26.46870576650871 27.927257061973044 23.787309988306973 42-43 21.252384762139208 26.52347836789956 27.52230906517324 24.70182780478799 44-45 20.5126469321189 26.040371715182474 28.331589636285308 25.115391716413317 46-47 20.638808542064126 25.951135454489503 28.73222967567235 24.677826327774017 48-49 19.703981783494367 25.99544587359222 29.83691304080251 24.463659302110898 50-51 19.563050033848235 26.375161548402982 29.18764231645024 24.874146101298543 52-53 19.09779063326974 26.229306418856545 29.756908117422608 24.915994830451105 54-55 18.709459043633455 26.754877223213736 29.111945350483108 25.4237183826697 56-57 18.713767001046218 26.631792725706198 29.011016062526924 25.64342421072066 58-59 19.09779063326974 26.340697889100866 28.68915010154471 25.872361376084683 60-61 19.02824789217798 26.4114714751677 28.647301372392146 25.91297926026217 62-63 18.95932057357376 26.461936119145797 28.695919748907624 25.882823558372824 64-65 19.83137423841467 26.22069050403102 27.670625884669825 26.27730937288448 66-67 19.85845282786633 26.05760354483353 26.993045725890823 27.090897901409317 68-69 19.85045233552834 26.095759739060863 26.531478860237552 27.52230906517324 70-71 21.180380331097297 25.24832297372146 26.37208443596529 27.199212259215948 72-73 21.171148993784232 25.791125607729708 25.51418548833774 27.523539910148315 74-75 21.435165240937902 25.943134962151515 25.350483106652717 27.27121669025786 76 21.950889285494494 24.998461443781157 25.125238476213923 27.92541079451043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 2.0 14 2.5 15 1.5 16 4.5 17 7.0 18 6.5 19 13.5 20 21.0 21 21.0 22 33.5 23 56.0 24 89.5 25 113.0 26 164.5 27 232.5 28 313.0 29 377.0 30 487.5 31 717.0 32 1024.0 33 1212.0 34 1421.5 35 1781.5 36 2107.5 37 2283.0 38 2636.5 39 3331.0 40 4187.0 41 4775.5 42 4849.0 43 5042.5 44 5539.5 45 5837.0 46 5831.0 47 5902.0 48 5671.0 49 5201.5 50 5034.0 51 4718.5 52 4055.0 53 3404.5 54 3102.0 55 2901.5 56 2365.5 57 1824.0 58 1618.0 59 1448.5 60 1095.0 61 797.5 62 684.0 63 579.0 64 417.5 65 309.5 66 244.5 67 231.0 68 171.5 69 99.5 70 67.5 71 48.0 72 38.5 73 21.5 74 12.5 75 11.0 76 12.5 77 8.5 78 5.5 79 8.0 80 7.5 81 3.5 82 2.0 83 4.0 84 3.5 85 3.0 86 4.0 87 5.0 88 3.0 89 2.0 90 1.5 91 0.5 92 1.0 93 0.5 94 1.0 95 1.0 96 0.0 97 0.5 98 2.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6265000923133731 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.44587359222106 #Duplication Level Percentage of deduplicated Percentage of total 1 71.97593624436699 39.90768662686934 2 12.606832863453727 13.979937226906273 3 6.09807534353009 10.143393439596284 4 3.085666082092037 6.843498061419165 5 1.7159855262281618 4.7572158286663795 6 1.0722134659355784 3.5669887377684786 7 0.7769662796634627 3.0155701889347037 8 0.6215730237307701 2.757092744168872 9 0.45064044220480837 2.248753769462736 >10 1.5961107287943703 12.779863376207768 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0024616899501507783 0.0 2 0.0 0.0 0.0 0.004923379900301557 0.0 3 0.0 0.0 0.0 0.004923379900301557 0.0 4 0.0 0.0 0.0 0.006154224875376946 0.0 5 0.0 0.0 0.0 0.006154224875376946 0.0 6 0.0 0.0 0.0 0.006154224875376946 0.0 7 0.0 0.0 0.0 0.006154224875376946 0.0 8 0.0 0.0 0.0 0.0073850698504523355 0.0 9 0.0 0.0 0.0 0.009846759800603113 0.0 10 0.0 0.0 0.0 0.01723182965105545 0.0 11 0.0 0.0 0.0 0.01846267462613084 0.0 12 0.0 0.0 0.0 0.020924364576281617 0.0 13 0.0 0.0 0.0 0.024616899501507784 0.0 14 0.0 0.0 0.0 0.02830943442673395 0.0 15 0.0 0.0 0.0 0.030771124376884732 0.0 16 0.0 0.0 0.0 0.03323281432703551 0.0 17 0.0 0.0 0.0 0.0344636593021109 0.0 18 0.0 0.0 0.0 0.03815619422733707 0.0 19 0.0 0.0 0.0 0.043079574127638624 0.0 20 0.0 0.0 0.0 0.045541264077789405 0.0 21 0.0 0.0 0.0 0.05538802387839251 0.0 22 0.0 0.0 0.0 0.059080558803618684 0.0 23 0.0 0.0 0.0 0.06646562865407102 0.0 24 0.0 0.0 0.0 0.07385069850452336 0.0 25 0.0 0.0 0.0 0.08123576835497569 0.0 26 0.0 0.0 0.0 0.08369745830512647 0.0 27 0.0 0.0 0.0 0.10708351283155887 0.0 28 0.0 0.0 0.0 0.12062280755738815 0.0 29 0.0 0.0 0.0 0.1600098467598006 0.0 30 0.0 0.0 0.0 0.19570435103698688 0.0 31 0.0 0.0 0.0 0.2289371653640224 0.0 32 0.0 0.0 0.0 0.25355406486553017 0.0 33 0.0 0.0 0.0 0.29171025909286724 0.0 34 0.0 0.0 0.0 0.3409440580958828 0.0 35 0.0 0.0 0.0 0.41848729152563235 0.0 36 0.0 0.0 0.0 0.5649578435596037 0.0 37 0.0 0.0 0.0 0.8677457074281494 0.0 38 0.0 0.0 0.0 1.2702320142778016 0.0 39 0.0 0.0 0.0 1.7625700043079575 0.0 40 0.0 0.0 0.0 2.46661333005108 0.0 41 0.0 0.0 0.0 3.2518924241491782 0.0 42 0.0 0.0 0.0 4.171333620530494 0.0 43 0.0 0.0 0.0 5.190473259892917 0.0 44 0.0 0.0 0.0 6.116068681149609 0.0 45 0.0 0.0 0.0 7.169671979814143 0.0 46 0.0 0.0 0.0 8.05095698196812 0.0 47 0.0 0.0 0.0 9.066404086405317 0.0 48 0.0 0.0 0.0 9.904609514431657 0.0 49 0.0 0.0 0.0 10.755123392208752 0.0 50 0.0 0.0 0.0 11.520708966705643 0.0 51 0.0 0.0 0.0 12.388454674133794 0.0 52 0.0 0.0 0.0 13.417441073296818 0.0 53 0.0 0.0 0.0 14.531355775740046 0.0 54 0.0 0.0 0.0 15.602190904055634 0.0 55 0.0 0.0 0.0 16.557326604714135 0.0 56 0.0 0.0 0.0 17.449689211643793 0.0 57 0.0 0.0 0.0 18.2595852052434 0.0 58 0.0 0.0 0.0 18.991937965413257 0.0 59 0.0 0.0 0.0 19.629515662502307 0.0 60 0.0 0.0 0.0 20.27447842944181 0.0 61 0.0 0.0 0.0 20.95636654563358 0.0 62 0.0 0.0 0.0 21.691180995753584 0.0 63 0.0 0.0 0.0 22.435842205674195 0.0 64 0.0 0.0 0.0 23.21127453997169 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15 0.0021644924 70.427505 1 ATTCTCG 15 0.002218219 69.99384 36 GCGCCAG 20 0.006931319 52.495384 54 TATTGCG 20 0.006931319 52.495384 50 CGTAGAT 20 0.006931319 52.495384 37 GAACCTA 20 0.006931319 52.495384 43 TCTATCG 20 0.006931319 52.495384 70 GTATAGG 35 0.0012494305 39.996483 3 GCTAGGT 50 0.0071746698 27.997538 21 TAACTAT 50 0.0071746698 27.997538 49 TATTATA 90 1.9086202E-4 23.33128 2 ATTATAC 90 1.9086202E-4 23.33128 3 CCTTACC 80 0.002647417 21.873075 8 CCCTATA 85 0.0037628715 20.586424 2 CTAGGAG 105 5.4099225E-4 19.998241 3 CTCCTAG 90 0.0050541987 19.563198 1 ACCATAC 95 0.0071581053 18.419432 8 AGCAGTC 135 1.3930189E-4 18.146553 70 ACGAGCA 265 3.362038E-8 15.847662 70 GAGCAGT 155 4.0002435E-4 15.805061 69 >>END_MODULE