FastQCFastQC Report
Wed 25 May 2016
SRR1780738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81245
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6470.7963566988737769No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5460.6720413563911626RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC900.11077604775678503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA150.002217444870.0000143
CCGATCA150.002217444870.0000134
TAGCCCG207.884395E-570.000015
TACTGAC252.817269E-670.0000134
GACTATC207.884395E-570.0000138
CCCGATC150.002217444870.0000133
GAAATCG252.3777815E-456.00000448
GCTGTCG252.3777815E-456.00000454
AGCGTTA200.006928907752.50000410
GGCAGTA200.006928907752.50000431
GGAGCGT200.006928907752.5000048
AGGAGCG200.006928907752.5000047
CGTGCGA200.006928907752.50000410
TCTTCCT200.006928907752.50000427
CCGTGCG200.006928907752.5000049
GAATATG200.006928907752.5000041
GATCAAC200.006928907752.50000436
GACCATA200.006928907752.5000046
TGGCAGT200.006928907752.50000430
CCCGTGC200.006928907752.5000048
Produced by FastQC (version 0.11.5)