##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780738_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81245 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49083635916056 34.0 34.0 34.0 31.0 34.0 2 33.182977413994706 34.0 34.0 34.0 32.0 34.0 3 33.39911379161794 34.0 34.0 34.0 33.0 34.0 4 33.34843990399409 34.0 34.0 34.0 33.0 34.0 5 33.333780540340946 34.0 34.0 34.0 33.0 34.0 6 37.02393993476522 38.0 37.0 38.0 36.0 38.0 7 36.687106898886086 38.0 37.0 38.0 34.0 38.0 8 36.60323712228445 38.0 37.0 38.0 34.0 38.0 9 36.47093359591359 38.0 37.0 38.0 34.0 38.0 10-11 36.11726260077543 38.0 36.5 38.0 32.5 38.0 12-13 36.00980983445135 38.0 37.0 38.0 32.0 38.0 14-15 36.290497876792415 38.0 37.0 38.0 33.5 38.0 16-17 36.480958828235586 38.0 37.0 38.0 34.0 38.0 18-19 36.44652594005785 38.0 37.0 38.0 34.0 38.0 20-21 36.155517262600775 38.0 37.0 38.0 33.0 38.0 22-23 36.18932241984122 38.0 37.0 38.0 33.0 38.0 24-25 35.416376392393374 37.5 35.5 38.0 29.0 38.0 26-27 35.687168441134844 38.0 36.0 38.0 31.5 38.0 28-29 35.53494984306727 38.0 36.0 38.0 31.0 38.0 30-31 35.178755615730196 37.5 35.5 38.0 29.0 38.0 32-33 35.767622622930645 38.0 36.5 38.0 31.5 38.0 34-35 35.815268631915814 38.0 36.5 38.0 32.5 38.0 36-37 36.00058465136316 38.0 37.0 38.0 33.0 38.0 38-39 35.68305126469321 38.0 36.5 38.0 32.5 38.0 40-41 35.01531786571481 37.0 35.5 38.0 29.0 38.0 42-43 35.441811803803304 37.5 36.0 38.0 29.5 38.0 44-45 35.67205981906579 38.0 36.0 38.0 31.5 38.0 46-47 35.99692288756231 38.0 37.0 38.0 33.0 38.0 48-49 35.74690134777525 38.0 36.5 38.0 31.5 38.0 50-51 35.97647239830144 38.0 37.0 38.0 32.5 38.0 52-53 35.887248446058216 38.0 37.0 38.0 32.0 38.0 54-55 36.048790694811984 38.0 37.0 38.0 33.5 38.0 56-57 36.130986522247525 38.0 37.0 38.0 34.0 38.0 58-59 36.21856114222413 38.0 37.0 38.0 34.0 38.0 60-61 35.8093482675857 38.0 36.5 38.0 32.5 38.0 62-63 36.11249307649702 38.0 37.0 38.0 33.0 38.0 64-65 36.14070404332574 38.0 37.0 38.0 34.0 38.0 66-67 35.437546925964675 38.0 36.0 38.0 31.0 38.0 68-69 34.82635854514124 37.5 35.5 38.0 27.0 38.0 70-71 34.83734999076866 37.0 35.0 38.0 27.0 38.0 72-73 34.515847129054094 37.0 34.5 38.0 25.5 38.0 74-75 35.274847682934336 37.5 36.0 38.0 30.0 38.0 76 34.67191827189365 37.0 35.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6554336377928465 1101 2 0.5555674751847164 1101 3 0.3611922463675654 1101 4 0.321069405144641 1101 5 0.23739578761671254 1101 6 -1.0176583248099078 1101 7 0.5099122073172424 1101 8 0.8177651304413587 1101 9 0.6356934593679355 1101 10-11 0.5356935381612544 1101 12-13 1.2597185051671644 1101 14-15 1.1049651236013602 1101 16-17 0.325689951785769 1101 18-19 0.008332065890407137 1101 20-21 0.17391476678720608 1101 22-23 0.8308318952653337 1101 24-25 -1.2297126831724086 1101 26-27 0.29288326716875446 1101 28-29 1.2728861103460218 1101 30-31 1.822362029644374 1101 32-33 0.7293480924211622 1101 34-35 0.5568635129390529 1101 36-37 0.5280395626603891 1101 38-39 0.3771944586825171 1101 40-41 1.8045397313408529 1101 42-43 1.1197722658907239 1101 44-45 0.27763555535662476 1101 46-47 0.5763363787456726 1101 48-49 -1.0560741585437654 1101 50-51 -0.7202298679187891 1101 52-53 -0.9552926607432255 1101 54-55 -0.07079621784330214 1101 56-57 -0.5534397162117131 1101 58-59 -0.6494379631789045 1101 60-61 -1.2671706528265645 1101 62-63 -0.29053093430708543 1101 64-65 -0.8457006526729032 1101 66-67 -1.5010838908312323 1101 68-69 -0.4407318288545241 1101 70-71 -0.6557416118066612 1101 72-73 -1.4501241761212569 1101 74-75 -0.843451093470378 1101 76 -0.4902035695497773 1102 1 -0.6715803256872839 1102 2 0.3838166695993266 1102 3 0.505553148623207 1102 4 0.6960067485030308 1102 5 0.4111695076310369 1102 6 0.24191919040670484 1102 7 0.10047790728144435 1102 8 0.5778259968932034 1102 9 0.7750776340904579 1102 10-11 0.9011876305350413 1102 12-13 0.6180876422956985 1102 14-15 0.10611084504783008 1102 16-17 0.2745889850832981 1102 18-19 -0.31662317936558537 1102 20-21 0.7429534348860258 1102 22-23 0.6565390202205776 1102 24-25 -4.706279099212505 1102 26-27 -0.8492846526307432 1102 28-29 0.6771288600775023 1102 30-31 1.9327093264578394 1102 32-33 0.6350050920631247 1102 34-35 0.841378371514061 1102 36-37 -2.0085787688827565 1102 38-39 0.40965954998220155 1102 40-41 1.2548440636000677 1102 42-43 1.1807819329154299 1102 44-45 1.384903725782685 1102 46-47 1.561746332200741 1102 48-49 -2.702977130258766 1102 50-51 -0.2543061300025684 1102 52-53 0.3060481913870987 1102 54-55 0.7160369364710562 1102 56-57 1.0199043582601846 1102 58-59 0.3944843425855069 1102 60-61 -5.87728522497121 1102 62-63 -2.190548836204684 1102 64-65 0.5041382302486852 1102 66-67 1.725706298928884 1102 68-69 -0.018605083419515722 1102 70-71 -0.31273230281991005 1102 72-73 -5.7903228871846295 1102 74-75 -1.6941403165280278 1102 76 0.6425390011627172 1103 1 0.8850178295890601 1103 2 0.5283151807475548 1103 3 -0.07746667852473621 1103 4 0.5537596717363868 1103 5 0.4357319304649394 1103 6 0.4444756578335358 1103 7 1.000281814609501 1103 8 0.7638380529611837 1103 9 0.48788175929925615 1103 10-11 0.4697401761084308 1103 12-13 -0.09920482629316041 1103 14-15 -0.18070010709103457 1103 16-17 0.4167416468597551 1103 18-19 0.5632736904143556 1103 20-21 0.38104625208407583 1103 22-23 0.5227529791137471 1103 24-25 -2.536121498805798 1103 26-27 0.08960706063819401 1103 28-29 0.28829161699520967 1103 30-31 1.4590293206476872 1103 32-33 1.4141829553792746 1103 34-35 0.8628977265870503 1103 36-37 -0.5564253808371475 1103 38-39 0.06948234030829781 1103 40-41 0.22974420159120967 1103 42-43 -0.3236628343153072 1103 44-45 1.102290609361738 1103 46-47 1.2747973889223587 1103 48-49 -1.440946481700415 1103 50-51 0.3853844793372545 1103 52-53 0.718249513196966 1103 54-55 0.37970169062894854 1103 56-57 0.7295564753347676 1103 58-59 0.12569939088241 1103 60-61 -3.5069315318925547 1103 62-63 -0.9824364097394351 1103 64-65 0.634445442101395 1103 66-67 0.6347483499129822 1103 68-69 0.8469624959253963 1103 70-71 1.0386221891548146 1103 72-73 -4.211057871569842 1103 74-75 -1.4150859189682876 1103 76 -2.598291850751 1104 1 0.29486804470565176 1104 2 0.4644441444698728 1104 3 0.42325037676819477 1104 4 0.07532444573117658 1104 5 -0.44851675493369214 1104 6 -0.1542123965564386 1104 7 0.4336487875738584 1104 8 0.44849306969247493 1104 9 1.1743161354611544 1104 10-11 -0.6804297925535963 1104 12-13 0.25160119697130057 1104 14-15 0.7289177289954409 1104 16-17 0.4428344922413814 1104 18-19 0.7624889132326516 1104 20-21 -0.1928370006911848 1104 22-23 0.3259876952129588 1104 24-25 0.4606185553717097 1104 26-27 1.109883790866057 1104 28-29 1.2363582234646415 1104 30-31 1.2438728963634205 1104 32-33 0.6141019546887776 1104 34-35 0.15777343700293756 1104 36-37 1.050787664257662 1104 38-39 1.3395864461137919 1104 40-41 1.8891495768501514 1104 42-43 0.4054164135733629 1104 44-45 0.7299463697059281 1104 46-47 1.101801160102049 1104 48-49 0.4326990805240456 1104 50-51 0.5240749239115345 1104 52-53 0.2652166348838989 1104 54-55 -0.11612876563068397 1104 56-57 0.2255927530210684 1104 58-59 -0.6900572352396495 1104 60-61 0.5806952806638819 1104 62-63 0.04250781929333414 1104 64-65 0.6031406670443005 1104 66-67 0.15772731629105152 1104 68-69 1.580085138938088 1104 70-71 1.9565259975151434 1104 72-73 1.8184343470523459 1104 74-75 0.19029465148903313 1104 76 -0.15353166591008005 1105 1 1.2356295655684875 1105 2 0.5457710864025458 1105 3 0.465302887934989 1105 4 0.25742489240293054 1105 5 0.015504380266222029 1105 6 0.5721739200357874 1105 7 0.9980348862774164 1105 8 -0.2857372386199799 1105 9 0.815998235907827 1105 10-11 0.7001672223717748 1105 12-13 -0.39156526996409013 1105 14-15 0.6902833461650673 1105 16-17 0.6371094677289122 1105 18-19 0.8366391474629538 1105 20-21 0.48082026044836823 1105 22-23 0.39947064542541 1105 24-25 0.7649409216425482 1105 26-27 0.6380459000282812 1105 28-29 1.0533317499925872 1105 30-31 1.632068778267758 1105 32-33 0.9408668528258275 1105 34-35 -0.5136640834241177 1105 36-37 0.9664919893475457 1105 38-39 0.30031201534790597 1105 40-41 0.7487067181509417 1105 42-43 0.8614165225176933 1105 44-45 1.361128960402084 1105 46-47 1.331074501424638 1105 48-49 0.18102406145879257 1105 50-51 1.0084005585122497 1105 52-53 0.7422838535351701 1105 54-55 1.1706399455579017 1105 56-57 0.930585126918011 1105 58-59 0.6834311621892653 1105 60-61 1.2945529993696212 1105 62-63 0.050773970290173054 1105 64-65 1.1256785251987793 1105 66-67 1.1777322187858843 1105 68-69 0.3432674028012528 1105 70-71 1.6165243633502087 1105 72-73 1.3825208488499285 1105 74-75 0.18364904739272703 1105 76 1.405461688485822 1106 1 -1.2239151579252656 1106 2 0.35351573566642713 1106 3 0.2956257878483868 1106 4 -0.012709958518755116 1106 5 0.4027987016815331 1106 6 0.4581073605900343 1106 7 -0.8421543998787087 1106 8 0.507625374271278 1106 9 0.21649928749758374 1106 10-11 0.789150273219235 1106 12-13 1.5180444046616657 1106 14-15 -7.812338176123035E-4 1106 16-17 0.40017701690619134 1106 18-19 0.7758015871511859 1106 20-21 0.798245721292119 1106 22-23 0.816596093897509 1106 24-25 -0.03182389340136638 1106 26-27 1.3924223691610251 1106 28-29 1.0518780818000835 1106 30-31 1.2914000908992094 1106 32-33 0.33580066452940116 1106 34-35 1.2200362753535572 1106 36-37 1.3997315043790906 1106 38-39 0.5622970456584326 1106 40-41 1.5398578996429606 1106 42-43 1.341003309601696 1106 44-45 1.5151095369207042 1106 46-47 1.3028826172232186 1106 48-49 1.2481556957529918 1106 50-51 0.6352563051409774 1106 52-53 0.8952128402975106 1106 54-55 0.5142468984219377 1106 56-57 0.6645947768004774 1106 58-59 0.8786247784208641 1106 60-61 1.3334463557966885 1106 62-63 0.8529049860021587 1106 64-65 -0.17554008175408597 1106 66-67 0.5642223833287474 1106 68-69 1.8508604994237885 1106 70-71 0.8125530655647282 1106 72-73 1.0790908104978314 1106 74-75 0.12692505877463844 1106 76 0.7438880153446661 1107 1 1.0106967956875792 1107 2 0.036770482703516905 1107 3 0.01532758471343243 1107 4 0.4087887033902504 1107 5 0.05757122621320576 1107 6 0.40088804110487786 1107 7 0.5581578213523315 1107 8 -0.12271106003483112 1107 9 -0.8480158138150244 1107 10-11 0.6230995531988412 1107 12-13 0.3634152470217984 1107 14-15 0.21524081282441898 1107 16-17 0.796554613439902 1107 18-19 0.8122167776698603 1107 20-21 0.7425237893436005 1107 22-23 0.24123289777159584 1107 24-25 -0.00853473831024587 1107 26-27 -0.4060515699239744 1107 28-29 0.975893629143016 1107 30-31 0.9150938592183095 1107 32-33 1.2531576162306948 1107 34-35 0.2698960943926849 1107 36-37 1.2833653771967235 1107 38-39 0.6732470902614693 1107 40-41 0.3577978767042609 1107 42-43 0.7701482057403481 1107 44-45 0.7074601167601315 1107 46-47 0.9760857521792587 1107 48-49 0.9558306033609938 1107 50-51 1.1246949441112903 1107 52-53 0.8395068380036435 1107 54-55 1.0103441330338896 1107 56-57 1.0486906096028221 1107 58-59 0.9862136658937786 1107 60-61 1.5229295978000081 1107 62-63 0.9939302185751231 1107 64-65 0.9381339337932602 1107 66-67 0.40148503147453596 1107 68-69 1.4182907275364443 1107 70-71 0.8727671601231606 1107 72-73 1.2763795832772402 1107 74-75 -0.4903809180317893 1107 76 0.7359869066406972 1108 1 -0.4914369169724466 1108 2 0.2697515841056699 1108 3 0.21752226059231816 1108 4 0.4813349338312207 1108 5 0.22826823901547755 1108 6 -0.05206867500972834 1108 7 0.21564613673538702 1108 8 -0.639272734491243 1108 9 -0.15862341389630785 1108 10-11 0.48877726098181284 1108 12-13 1.367784277706619 1108 14-15 0.747754529548665 1108 16-17 0.17701590179592586 1108 18-19 0.4672157616162096 1108 20-21 1.0740620945661163 1108 22-23 0.4120823186210174 1108 24-25 1.2392807463473403 1108 26-27 0.09318638984029803 1108 28-29 -0.3293796892882028 1108 30-31 0.7822956474727292 1108 32-33 1.2152588151739963 1108 34-35 1.0562708149779283 1108 36-37 1.5117742371845324 1108 38-39 1.221408033159257 1108 40-41 1.1863388236662615 1108 42-43 0.4375938468702003 1108 44-45 0.8933979342768978 1108 46-47 0.7650006068835822 1108 48-49 1.4527316397357168 1108 50-51 0.9427705385026641 1108 52-53 -0.11248462714571872 1108 54-55 -0.2845146355090904 1108 56-57 -0.12190479783468788 1108 58-59 0.5280750761762434 1108 60-61 1.2017550397833432 1108 62-63 1.088270799757808 1108 64-65 1.010426150822319 1108 66-67 1.409765868702742 1108 68-69 1.779243785857929 1108 70-71 -0.11300808880998403 1108 72-73 0.45032796775193873 1108 74-75 -0.3860322084199197 1108 76 1.4362510805890878 1109 1 -1.3803932890748882 1109 2 0.18586512943269895 1109 3 0.5346596226735016 1109 4 0.45866057407820904 1109 5 0.19871059148030668 1109 6 -0.32713989390117604 1109 7 -0.3826070120795606 1109 8 0.5422378290417953 1109 9 0.9852893592299523 1109 10-11 0.4876204058923719 1109 12-13 0.5198456488691221 1109 14-15 0.8709812876068739 1109 16-17 0.7248373990556232 1109 18-19 1.0232147782893861 1109 20-21 0.8742153778654682 1109 22-23 0.5581135826298151 1109 24-25 0.2222388248110363 1109 26-27 0.8444553152665293 1109 28-29 -0.5403900576019396 1109 30-31 0.7205485070739073 1109 32-33 0.9614592692396116 1109 34-35 0.27916786933565874 1109 36-37 0.9718844276318066 1109 38-39 1.1743312613633847 1109 40-41 0.9971279434441485 1109 42-43 1.144215396188379 1109 44-45 0.9238015320962276 1109 46-47 1.1431114625225263 1109 48-49 1.0753076668061254 1109 50-51 0.3026609264829432 1109 52-53 -0.1387886197418453 1109 54-55 -0.6242902056217403 1109 56-57 -0.017021423288390736 1109 58-59 -0.07603465152899247 1109 60-61 0.9713962700987295 1109 62-63 0.02954594328014082 1109 64-65 1.0265642793199987 1109 66-67 0.5282133691727111 1109 68-69 0.2045470988016902 1109 70-71 0.43072103357081915 1109 72-73 0.2169613748347814 1109 74-75 0.16022030412473498 1109 76 0.5554794582444273 1110 1 -1.3977507938745575 1110 2 -0.474286571697192 1110 3 0.03412457719463191 1110 4 0.09012439556185825 1110 5 0.5022859542096683 1110 6 0.0048243524715303465 1110 7 0.48319099251553865 1110 8 0.6455330797263414 1110 9 0.8881628681840965 1110 10-11 -0.5792445423341093 1110 12-13 0.5736098511792562 1110 14-15 0.8504685881450698 1110 16-17 0.46528526359470135 1110 18-19 -0.14123302330316534 1110 20-21 0.8495560759424521 1110 22-23 -0.4289351694552934 1110 24-25 -1.024346326103263 1110 26-27 0.9477741708986684 1110 28-29 0.7163845623584066 1110 30-31 -0.14196054148933968 1110 32-33 0.7100351040679271 1110 34-35 0.9723507604696451 1110 36-37 1.3490442803369334 1110 38-39 0.8429728753123342 1110 40-41 -1.4116625390409823 1110 42-43 0.12507965200306614 1110 44-45 0.6680970030936138 1110 46-47 0.5971604978670086 1110 48-49 1.4717527616980135 1110 50-51 -0.28065667021865437 1110 52-53 0.16193054432827836 1110 54-55 -0.6912259686910076 1110 56-57 0.5897122814294491 1110 58-59 -0.7643391868262555 1110 60-61 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-1.2895254872065252 2116 52-53 0.2603681229525421 2116 54-55 -0.7693596173600596 2116 56-57 -0.25690121426823254 2116 58-59 -2.4963625350877123 2116 60-61 -2.0418581199974852 2116 62-63 0.20692152918697815 2116 64-65 -1.9898859662562671 2116 66-67 -1.300970958800555 2116 68-69 -2.3578449709913087 2116 70-71 0.27160107124828414 2116 72-73 -0.06133596307366673 2116 74-75 -0.6002138836794373 2116 76 -1.375669479834471 2117 1 1.2450495695140376 2117 2 0.5477438620979456 2117 3 -0.1676128745428187 2117 4 -0.6335299310336424 2117 5 -0.035245274498031165 2117 6 0.2741861512450967 2117 7 0.5090824301716808 2117 8 0.16864232342381769 2117 9 -1.3217508270237204 2117 10-11 -0.4146976424930884 2117 12-13 -1.8088566489343094 2117 14-15 -0.2527280959339677 2117 16-17 -1.537752437972408 2117 18-19 -1.5573981379976871 2117 20-21 -0.36271396720994176 2117 22-23 0.43621359275229565 2117 24-25 -2.0575339254673324 2117 26-27 -3.145713224059314 2117 28-29 -1.9900939750024804 2117 30-31 -0.518380028202948 2117 32-33 -3.0627334628182865 2117 34-35 0.1441086528157669 2117 36-37 -1.7536697782594786 2117 38-39 -0.5305224687712453 2117 40-41 -0.46810133077389793 2117 42-43 -0.0870200527436964 2117 44-45 -0.35370631282734877 2117 46-47 -1.6746712077882293 2117 48-49 -1.3532529162488416 2117 50-51 -0.7367661410340105 2117 52-53 -5.155151766089716E-4 2117 54-55 -0.6802675312619826 2117 56-57 0.1860102600803728 2117 58-59 -1.3334114103102408 2117 60-61 -1.1216040335757285 2117 62-63 -0.8446442581572455 2117 64-65 -1.4070448916487237 2117 66-67 -0.9106974517605764 2117 68-69 -0.07693382031968099 2117 70-71 -1.9407107915126858 2117 72-73 0.41171792914190064 2117 74-75 0.55460485721715 2117 76 0.3513089956470026 2118 1 -0.2906375260322136 2118 2 0.17602707488820357 2118 3 -1.4497788433285024 2118 4 0.1541556418631984 2118 5 0.3262887947825135 2118 6 -0.10228891537081353 2118 7 0.35986278799428817 2118 8 0.3623042952548019 2118 9 -2.447083906582151 2118 10-11 -1.0709595381916017 2118 12-13 -1.8133294315761006 2118 14-15 0.19583490368537326 2118 16-17 -3.5808148665982245 2118 18-19 -0.8055424090292789 2118 20-21 -0.4725826386983414 2118 22-23 0.13006589575952887 2118 24-25 0.8932178826788402 2118 26-27 -2.820008998707202 2118 28-29 -1.4317098866888216 2118 30-31 -1.9087968534789255 2118 32-33 -0.6760756782731789 2118 34-35 -0.8425681648469592 2118 36-37 -4.508808720018138 2118 38-39 -0.7767158452463079 2118 40-41 -1.3107926136060186 2118 42-43 -0.16729222632573482 2118 44-45 -3.3850157913199226 2118 46-47 -0.5055143748990076 2118 48-49 -0.49590613284571816 2118 50-51 -1.5097962133598557 2118 52-53 -2.129464887083728 2118 54-55 -1.6553341471736687 2118 56-57 0.010620081169058437 2118 58-59 -1.2221152549999985 2118 60-61 -1.5604125604124306 2118 62-63 -2.102305088001181 2118 64-65 -0.7784285993000353 2118 66-67 -0.30782345099925834 2118 68-69 -2.3167511023904837 2118 70-71 -0.8296049673786925 2118 72-73 0.531436239001053 2118 74-75 -0.32915037689019755 2118 76 -0.6989384348821233 2119 1 0.5056105506632562 2119 2 -1.852437832201396 2119 3 -0.35663172532637333 2119 4 -1.5275226632616707 2119 5 -0.711999760076047 2119 6 -0.6632428744192254 2119 7 -0.6428594276525317 2119 8 -0.11076944128206634 2119 9 -0.5151984763537243 2119 10-11 -0.8229420213257015 2119 12-13 -0.2772304849551972 2119 14-15 -0.7104557816028247 2119 16-17 -1.3939584338842863 2119 18-19 -0.6790481493535836 2119 20-21 -2.3689313190155303 2119 22-23 -0.29448138794268175 2119 24-25 0.5239730016278301 2119 26-27 0.15155568350370885 2119 28-29 -0.7079851855407497 2119 30-31 0.8328293744770505 2119 32-33 -0.4202169967724103 2119 34-35 0.6320146260158594 2119 36-37 -0.7938128625202054 2119 38-39 -2.1885397297296834 2119 40-41 -2.142754976015773 2119 42-43 -1.085004381610382 2119 44-45 -0.42854165236857256 2119 46-47 -0.020486560956527455 2119 48-49 -1.467322868554092 2119 50-51 0.2256458507897534 2119 52-53 -0.20991819516751775 2119 54-55 0.01255554391402569 2119 56-57 -1.9754137689907196 2119 58-59 -1.3525744809439004 2119 60-61 -0.8793260412977375 2119 62-63 -0.7865872515708361 2119 64-65 -1.098022634097049 2119 66-67 -0.6864185510110943 2119 68-69 -0.3529388169129106 2119 70-71 -1.3678343436362397 2119 72-73 0.19743221485618534 2119 74-75 -1.2462411568641585 2119 76 -1.6107623193654916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 6.0 22 10.0 23 21.0 24 51.0 25 90.0 26 144.0 27 332.0 28 508.0 29 918.0 30 1364.0 31 2294.0 32 3630.0 33 5518.0 34 8245.0 35 12415.0 36 19940.0 37 25757.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.47005969598129 21.762570004307957 11.787802326297003 27.97956797341375 2 18.71622869099637 18.994399655363406 36.93765770201243 25.35171395162779 3 18.588220813588528 23.9460889900917 29.129177180134164 28.33651301618561 4 13.784232875869284 17.959259031325004 37.5493876546249 30.707120438180812 5 13.574989230106468 36.66810265247092 36.1339159332882 13.622992184134407 6 30.24555357252754 37.6995507415841 16.9942765708659 15.060619115022464 7 29.011016062526924 29.191950273863004 22.328758692842637 19.468274970767432 8 24.883992861099145 35.180011077604775 19.841220998215274 20.094775063080807 9 23.70484337497692 15.698196812111515 21.799495353560218 38.797464459351346 10-11 25.007077358606683 25.668656532709704 27.530309557511227 21.793956551172382 12-13 25.14985537571543 22.804480275709274 26.870576650870824 25.175087697704473 14-15 22.54723367591852 25.05938827004739 23.28881777340144 29.10456028063265 16-17 22.493076497015203 28.08788233122038 25.728967936488402 23.690073235276017 18-19 22.596467474921532 27.131515785586807 26.94873530678811 23.323281432703553 20-21 21.81980429564896 27.27429380269555 26.475475413871624 24.430426487783862 22-23 22.630931134223644 27.77032432765093 27.216444088867007 22.382300449258416 24-25 22.719551972429073 28.273739922456762 26.59548279894147 22.41122530617269 26-27 21.87396147455228 27.993722690627116 27.812788479291033 22.31952735552957 28-29 21.6604098713767 26.432395839743982 29.137177672472152 22.770016616407162 30-31 22.171210536032987 26.674872299833837 28.881161917656474 22.272755246476706 32-33 21.80257246599791 27.267524155332634 27.07982029663364 23.850083082035816 34-35 21.95212013046957 26.740107083512832 28.37774632285064 22.930026463166964 36-37 22.209366730260324 27.663240814819375 27.22629084866761 22.90110160625269 38-39 22.035817588774695 27.4318419595052 27.68970398178349 22.84263646993661 40-41 21.90657886639178 26.228690996369007 28.67561080681888 23.189119330420333 42-43 22.641393316511785 25.67788787002277 28.66761031448089 23.013108498984554 44-45 20.83882085051388 26.208382054280264 29.196873653763312 23.75592344144255 46-47 21.91704104867992 25.224321496707493 29.18025724659979 23.6783802080128 48-49 20.64865530186473 25.213859314419352 30.03507908178965 24.102406301926273 50-51 19.902763246969045 25.559726752415536 30.16308695919749 24.374423041417934 52-53 19.67259523662995 25.692658009723672 31.350852360145236 23.28389439350114 54-55 18.8146962890024 25.924056865037848 30.49972305988061 24.76152378607914 56-57 19.665825589267033 25.738814696289005 29.728598682995877 24.86676103144809 58-59 18.586374546125917 26.757954335651423 29.635669887377684 25.020001230844972 60-61 18.692842636469937 27.367838020801283 28.939011631485013 25.000307711243767 62-63 18.18511908425134 28.146962890024003 28.030032617391836 25.63788540833282 64-65 18.893470367407225 28.759923687611543 26.368391901040063 25.97821404394117 66-67 18.68607298910702 28.56114222413687 25.830512646932117 26.92227213982399 68-69 18.820850513877776 28.180811126838574 25.901286232998956 27.097052126284694 70-71 19.012246907502 27.47184442119515 25.97452150901594 27.541387162286913 72-73 19.230721890577883 26.75118468828851 26.19053480214167 27.827558618991937 74-75 19.740291710259093 26.818881161917655 26.325312326912425 27.115514800910823 76 20.124315342482614 26.34992922641393 26.716721028986402 26.809034402117053 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.5 16 3.0 17 5.0 18 6.5 19 11.0 20 23.5 21 33.0 22 39.5 23 49.0 24 72.5 25 93.0 26 146.5 27 226.0 28 322.0 29 392.0 30 538.0 31 762.5 32 1031.0 33 1221.0 34 1361.0 35 1777.5 36 2235.0 37 2416.0 38 2791.0 39 3640.0 40 4592.5 41 5229.5 42 5388.0 43 5512.5 44 5803.0 45 6111.5 46 6254.0 47 6157.0 48 5741.0 49 5135.0 50 4848.0 51 4543.5 52 3818.0 53 3108.5 54 2820.0 55 2637.0 56 2124.0 57 1639.0 58 1484.0 59 1235.5 60 821.0 61 592.0 62 529.0 63 457.5 64 340.5 65 238.0 66 154.5 67 128.0 68 101.5 69 57.0 70 27.5 71 16.0 72 14.5 73 10.0 74 4.5 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81245.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.69031940427103 #Duplication Level Percentage of deduplicated Percentage of total 1 65.10349455427658 31.048064496276695 2 15.451917617302433 14.738137731552712 3 7.311722500387137 10.460951443165733 4 3.8300727817064986 7.306295772047511 5 2.260878542301141 5.391100990830204 6 1.3988540752593817 4.002707858945167 7 0.9730036648944408 3.2481998892239523 8 0.7458834460331389 2.8457135823743 9 0.62458060186858 2.6807803557141976 >10 2.284106746502865 16.458859006708103 >50 0.010323646311877356 0.350790817896486 >100 0.0 0.0 >500 0.005161823155938678 1.4683980552649394 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 647 0.7963566988737769 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 546 0.6720413563911626 RNA PCR Primer, Index 48 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 90 0.11077604775678503 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0024616899501507783 0.0 2 0.0 0.0 0.0 0.0024616899501507783 0.0 3 0.0 0.0 0.0 0.0024616899501507783 0.0 4 0.0 0.0 0.0 0.004923379900301557 0.0 5 0.0 0.0 0.0 0.004923379900301557 0.0 6 0.0 0.0 0.0 0.004923379900301557 0.0 7 0.0 0.0 0.0 0.004923379900301557 0.0 8 0.0 0.0 0.0 0.006154224875376946 0.0 9 0.0 0.0 0.0 0.009846759800603113 0.0 10 0.0 0.0 0.0 0.01600098467598006 0.0 11 0.0 0.0 0.0 0.01723182965105545 0.0 12 0.0 0.0 0.0 0.01846267462613084 0.0 13 0.0 0.0 0.0 0.022155209551357007 0.0 14 0.0 0.0 0.0 0.024616899501507784 0.0 15 0.0 0.0 0.0 0.027078589451658565 0.0 16 0.0 0.0 0.0 0.030771124376884732 0.0 17 0.0 0.0 0.0 0.03200196935196012 0.0 18 0.0 0.0 0.0 0.03569450427718629 0.0 19 0.0 0.0 0.0 0.041848729152563234 0.0 20 0.0 0.0 0.0 0.044310419102714015 0.0 21 0.0 0.0 0.0 0.04800295402794018 0.0 22 0.0 0.0 0.0 0.05169548895316635 0.0 23 0.0 0.0 0.0 0.059080558803618684 0.0 24 0.0 0.0 0.0 0.06646562865407102 0.0 25 0.0 0.0 0.0 0.07261985352944797 0.0 26 0.0 0.0 0.0 0.07631238845467414 0.0 27 0.0 0.0 0.0 0.09969844298110653 0.0 28 0.0 0.0 0.0 0.1132377377069358 0.0 29 0.0 0.0 0.0 0.14770139700904672 0.0 30 0.0 0.0 0.0 0.183395901286233 0.0 31 0.0 0.0 0.0 0.21416702566311774 0.0 32 0.0 0.0 0.0 0.2400147701397009 0.0 33 0.0 0.0 0.0 0.27694011939196256 0.0 34 0.0 0.0 0.0 0.32863560834512895 0.0 35 0.0 0.0 0.0 0.40371715182472767 0.0 36 0.0 0.0 0.0 0.5588036186842267 0.0 37 0.0 0.0 0.0 0.8640531725029232 0.0 38 0.0 0.0 0.0 1.245615114776294 0.0 39 0.0 0.0 0.0 1.7207212751553942 0.0 40 0.0 0.0 0.0 2.419841220998215 0.0 41 0.0 0.0 0.0 3.2174287648470674 0.0 42 0.0 0.0 0.0 4.14302418610376 0.0 43 0.0 0.0 0.0 5.164625515416334 0.0 44 0.0 0.0 0.0 6.127146285925288 0.0 45 0.0 0.0 0.0 7.153670995138162 0.0 46 0.0 0.0 0.0 8.041110222167518 0.0 47 0.0 0.0 0.0 9.067634931380393 0.0 48 0.0 0.0 0.0 9.921841344082713 0.0 49 0.0 0.0 0.0 10.778509446735184 0.0 50 0.0 0.0 0.0 11.565019385808357 0.0 51 0.0 0.0 0.0 12.459843682688165 0.0 52 0.0 0.0 0.0 13.503600221552096 0.0 53 0.0 0.0 0.0 14.619976613945473 0.0 54 0.0 0.0 0.0 15.744968921164379 0.0 55 0.0 0.0 0.0 16.71979814142409 0.0 56 0.0 0.0 0.0 17.619545818204198 0.0 57 0.0 0.0 0.0 18.44421195150471 0.0 58 0.0 0.0 0.0 19.16425626192381 0.0 59 0.0 0.0 0.0 19.83137423841467 0.0 60 0.0 0.0 0.0 20.46402855560342 0.0 61 0.0 0.0 0.0 21.158225121545943 0.0 62 0.0 0.0 0.0 21.884423656840422 0.0 63 0.0 0.0 0.0 22.63770078158656 0.0 64 0.0 0.0 0.0 23.387285371407472 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAATA 15 0.0022174448 70.00001 43 CCGATCA 15 0.0022174448 70.00001 34 TAGCCCG 20 7.884395E-5 70.00001 5 TACTGAC 25 2.817269E-6 70.00001 34 GACTATC 20 7.884395E-5 70.00001 38 CCCGATC 15 0.0022174448 70.00001 33 GAAATCG 25 2.3777815E-4 56.000004 48 GCTGTCG 25 2.3777815E-4 56.000004 54 AGCGTTA 20 0.0069289077 52.500004 10 GGCAGTA 20 0.0069289077 52.500004 31 GGAGCGT 20 0.0069289077 52.500004 8 AGGAGCG 20 0.0069289077 52.500004 7 CGTGCGA 20 0.0069289077 52.500004 10 TCTTCCT 20 0.0069289077 52.500004 27 CCGTGCG 20 0.0069289077 52.500004 9 GAATATG 20 0.0069289077 52.500004 1 GATCAAC 20 0.0069289077 52.500004 36 GACCATA 20 0.0069289077 52.500004 6 TGGCAGT 20 0.0069289077 52.500004 30 CCCGTGC 20 0.0069289077 52.500004 8 >>END_MODULE