FastQCFastQC Report
Wed 25 May 2016
SRR1780737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199600
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTCG250.002361621535.1731133
TTGCGAT701.0168151E-931.48070316
TAACGCC300.00577824829.298716
TACGCTT300.00577824829.298715
GCGAACC407.0309255E-427.50078411
CATCGTT1350.026.05307232
TTGTTCG959.767973E-1025.4446779
GCGAACT1350.024.42882728
AAAGCGA1450.024.26642425
CGTGCGA1001.7444108E-924.2006910
CGGTATG852.3757639E-723.270543
TCGTTGT1550.022.71601334
TCGTTCG1004.606227E-822.00797323
ATTGTCG602.8851992E-421.98594726
GACTATC2500.021.1258938
CCAACGT1158.738425E-921.05022622
GTATCGA1057.74362E-820.95297611
CCGTGCG1401.8735591E-1020.4053089
GTACAAG1750.020.1207351
GTACATG2300.020.0933971
Produced by FastQC (version 0.11.5)