FastQCFastQC Report
Wed 25 May 2016
SRR1780737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199600
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT196971.6419639879959986No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT82590.6884794931643882Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC34490.287512504168056No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG17150.14296432144048016No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC14740.12287429143047683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT9100.036.26400844
GCCGTCT9900.033.33358843
TCGTTCG900.031.77536823
TCTTTCG353.2153452E-431.43274113
AGAACGG300.005744783729.33355342
CATAGCG300.005744783729.33355335
ACTCGAC300.005744783729.33355340
TGCCGTC11900.027.73130242
ATGCCGT31500.027.51767244
CGTTCGG1053.6379788E-1227.2360324
CGTGCGA1650.026.67020410
TATGCCG33000.026.200243
CGAGAAT1750.025.14619414
TATACCG1850.024.971086
TTCGGTA1151.2732926E-1124.86768226
CCCGTGC2050.024.6810558
GGTCAAA450.001397127524.44768511
TCGCCAG901.5124897E-824.4446336
TGCCGAG551.5921747E-424.00318511
CGGTAAG1202.3646862E-1123.83152828
Produced by FastQC (version 0.11.5)