FastQCFastQC Report
Wed 25 May 2016
SRR1780736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59401
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTT150.002215454669.99242426
TCGTTCG150.002215454669.99242423
AGGACGG150.002215454669.99242428
CGTTCGG150.002215454669.99242424
TCTAACT150.002215454669.99242451
GGTATTG150.002215454669.99242456
AGGGGCG150.002215454669.99242446
GTGAAAC150.002215454669.99242441
TCGGTTC150.002215454669.99242413
GGGGCGT150.002215454669.99242447
CAGTCTA252.3738304E-455.99393519
TAGAGTA200.006922750752.49431626
CGGTTCA200.006922750752.49431614
AAGCATT200.006922750752.49431632
CTGCACA200.006922750752.49431640
TTCGGTA200.006922750752.49431626
TAGGACG200.006922750752.49431627
CGTTTGG200.006922750752.49431651
TGGCTGC200.006922750752.49431667
TCGGTAA200.006922750752.49431627
Produced by FastQC (version 0.11.5)