##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780736_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59401 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.82660224575344 34.0 31.0 34.0 12.0 34.0 2 31.090857056278512 34.0 32.0 34.0 23.0 34.0 3 31.36810828100537 34.0 33.0 34.0 23.0 34.0 4 31.776232723354827 34.0 34.0 34.0 23.0 34.0 5 31.658827292469823 34.0 34.0 34.0 23.0 34.0 6 33.890321711755696 37.0 34.0 38.0 23.0 38.0 7 33.50686015386946 37.0 34.0 38.0 21.0 38.0 8 32.255063046076664 37.0 31.0 38.0 11.0 38.0 9 33.2658036059999 37.0 34.0 38.0 21.0 38.0 10-11 33.729928789077626 37.0 34.0 38.0 22.0 38.0 12-13 34.03990673557684 37.0 34.0 38.0 23.0 38.0 14-15 33.74061884480059 37.0 34.0 38.0 21.5 38.0 16-17 33.62594064072995 37.0 34.0 38.0 21.0 38.0 18-19 33.59034359690914 37.0 34.0 38.0 21.5 38.0 20-21 33.66125991144931 37.0 34.0 38.0 21.0 38.0 22-23 33.58613491355365 37.0 34.0 38.0 21.5 38.0 24-25 34.11774212555344 37.5 34.5 38.0 23.0 38.0 26-27 34.19392771165469 38.0 34.5 38.0 23.0 38.0 28-29 34.42524536623962 38.0 35.0 38.0 24.0 38.0 30-31 34.300407400548806 38.0 35.0 38.0 24.0 38.0 32-33 33.87772933115604 37.0 34.0 38.0 22.0 38.0 34-35 33.907190114644536 37.0 34.0 38.0 23.0 38.0 36-37 33.87775458325618 37.0 34.0 38.0 22.5 38.0 38-39 34.40893250955371 38.0 35.0 38.0 24.0 38.0 40-41 33.85452265113382 37.5 34.0 38.0 22.0 38.0 42-43 34.16992138179492 37.0 34.0 38.0 23.5 38.0 44-45 33.11860911432467 37.0 33.5 38.0 18.5 38.0 46-47 33.89232504503291 37.0 34.0 38.0 23.0 38.0 48-49 33.677749532836145 37.0 34.0 38.0 22.5 38.0 50-51 33.4809430817663 37.0 34.0 38.0 22.0 38.0 52-53 33.52157371088029 37.0 34.0 38.0 21.5 38.0 54-55 33.20072052659046 37.0 33.5 38.0 21.0 38.0 56-57 33.285239305735594 37.0 33.5 38.0 21.5 38.0 58-59 33.191621353175876 37.0 33.0 38.0 21.5 38.0 60-61 33.21049308934193 37.0 32.5 38.0 21.5 38.0 62-63 33.243607009982995 37.0 33.0 38.0 21.5 38.0 64-65 33.31345431895086 37.0 33.0 38.0 21.5 38.0 66-67 33.264162219491254 37.0 33.0 38.0 21.0 38.0 68-69 32.70800154879548 37.0 31.5 38.0 16.0 38.0 70-71 32.719449167522434 37.0 32.0 38.0 19.0 38.0 72-73 32.85812528408613 37.0 32.0 38.0 19.5 38.0 74-75 32.33893368798505 37.0 31.0 38.0 15.0 38.0 76 31.491001834985944 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.8379163058868642 1101 2 -0.9826427068661623 1101 3 0.11451664609388601 1101 4 -1.8279997125001657 1101 5 -0.3175740996227816 1101 6 -1.6893614082119939 1101 7 -0.41775345392920826 1101 8 0.013796086180576594 1101 9 0.2294489933808208 1101 10-11 -0.6515775850034373 1101 12-13 -0.11928164884022863 1101 14-15 0.31487672388680465 1101 16-17 -1.9460883274475762 1101 18-19 -0.6050825407056735 1101 20-21 -0.47015962796462674 1101 22-23 -0.3971226359387643 1101 24-25 -0.8133268334033943 1101 26-27 -0.8939050040649335 1101 28-29 -0.1845966353111379 1101 30-31 -0.6961449801439059 1101 32-33 0.7758690953152865 1101 34-35 -0.6401708268982915 1101 36-37 0.23751557383138788 1101 38-39 -0.33136553052261064 1101 40-41 0.6186948085080317 1101 42-43 -0.017634755081509468 1101 44-45 0.8043749921176442 1101 46-47 0.9727968178872075 1101 48-49 -0.8477972048038609 1101 50-51 -0.8546976683423892 1101 52-53 0.3020053231646074 1101 54-55 -1.2089662255292524 1101 56-57 -0.4416143984348153 1101 58-59 -0.18521935837823378 1101 60-61 0.33723705711926755 1101 62-63 -1.520719349101018 1101 64-65 0.0978587941740443 1101 66-67 0.0497045946960597 1101 68-69 1.1378606787124426 1101 70-71 0.5266262466716825 1101 72-73 0.3709715719052724 1101 74-75 -1.0378113560628606 1101 76 -2.0502337721242014 1102 1 0.6878600916286643 1102 2 -0.7985743838847945 1102 3 0.3219390063423333 1102 4 0.15137916948740937 1102 5 0.6227364593834288 1102 6 1.5445826911668874 1102 7 1.8053210802322823 1102 8 1.3819327321433121 1102 9 0.6063123474180898 1102 10-11 -0.39134466574878246 1102 12-13 -3.399033201635259 1102 14-15 -0.48787172331816464 1102 16-17 -2.9403429858326717 1102 18-19 -0.3676291245566077 1102 20-21 0.6534894403583564 1102 22-23 -0.787774809851804 1102 24-25 0.3503688187705265 1102 26-27 -5.9793863705245585 1102 28-29 -0.4402177533235587 1102 30-31 0.42596682109832074 1102 32-33 -6.5027333891567665 1102 34-35 -0.8542080939790324 1102 36-37 0.08386650550840358 1102 38-39 0.6564295005332923 1102 40-41 0.25038735509188115 1102 42-43 0.5486851206948913 1102 44-45 -5.79290761657801 1102 46-47 -0.2680572193798767 1102 48-49 0.6011624225253343 1102 50-51 -6.910039283249223 1102 52-53 -1.2519853600652056 1102 54-55 0.72783501670677 1102 56-57 -0.9788038394286076 1102 58-59 0.8561781571497136 1102 60-61 0.6569886099142934 1102 62-63 -4.471977113076175 1102 64-65 -0.3297343735278204 1102 66-67 0.4918319238886113 1102 68-69 -7.279980936194384 1102 70-71 -0.0866967346947689 1102 72-73 0.5627728141412973 1102 74-75 -6.332097070348574 1102 76 -2.0989294242981167 1103 1 0.11955877716961538 1103 2 -0.03858955071998338 1103 3 -0.4086727226768794 1103 4 -0.08886349987558262 1103 5 0.1184391894137633 1103 6 -1.0181321723315975 1103 7 0.27208043311397745 1103 8 1.8553907705659611 1103 9 0.5776217508559043 1103 10-11 0.8193858701460641 1103 12-13 -1.0443314827272658 1103 14-15 -0.5870501864121991 1103 16-17 -1.0570517825970782 1103 18-19 0.05598058221791291 1103 20-21 -0.21793371434336706 1103 22-23 -0.07526217082247655 1103 24-25 0.6271382828756771 1103 26-27 -0.6570279276529689 1103 28-29 0.5457023680111206 1103 30-31 0.5920942225139001 1103 32-33 -4.866257150531894 1103 34-35 -1.0111342820477915 1103 36-37 0.20296407881477307 1103 38-39 0.6694982568528047 1103 40-41 0.32115328026883105 1103 42-43 0.5263519558819425 1103 44-45 -5.29097384509166 1103 46-47 0.27631841257159095 1103 48-49 0.5981164164586019 1103 50-51 0.6185249230197414 1103 52-53 -0.3072305572340639 1103 54-55 0.7767480601850352 1103 56-57 1.0962593557180043 1103 58-59 1.0205613725187774 1103 60-61 1.1487985189132814 1103 62-63 0.6744965977428308 1103 64-65 0.519519923742152 1103 66-67 0.22983748506150903 1103 68-69 -2.68426556713473 1103 70-71 0.8720747516550773 1103 72-73 1.7645928343637465 1103 74-75 -1.2739575354648522 1103 76 0.8725569255502172 1104 1 -2.1535690741712123 1104 2 0.30312626100807094 1104 3 -0.11640611535518985 1104 4 0.11830240643353207 1104 5 -0.36192133133843285 1104 6 0.09828042364036094 1104 7 0.805295076487738 1104 8 1.2934679932209079 1104 9 0.1750402442352339 1104 10-11 0.07889404862608984 1104 12-13 -0.20733359689217679 1104 14-15 0.18436407863731574 1104 16-17 -0.20077048739289438 1104 18-19 0.4802489698889971 1104 20-21 1.3690474807161692 1104 22-23 0.5986668377454478 1104 24-25 0.6705009177927792 1104 26-27 0.8544887074866807 1104 28-29 -0.38776820058796346 1104 30-31 -0.4577444333223113 1104 32-33 -0.8320188062568263 1104 34-35 0.4044771566970624 1104 36-37 0.19247114457643022 1104 38-39 -0.244366214049613 1104 40-41 0.97455263489217 1104 42-43 0.7358990795049536 1104 44-45 -3.343471897834508 1104 46-47 0.4008761070191298 1104 48-49 1.0443832463239673 1104 50-51 1.6143522291285635 1104 52-53 0.14955146123705987 1104 54-55 -0.13234284890587134 1104 56-57 1.0793015277442635 1104 58-59 1.2374190558391263 1104 60-61 0.23801627789848112 1104 62-63 1.0047625374334999 1104 64-65 0.14144047565046236 1104 66-67 0.25781122490795383 1104 68-69 -3.4969171750127757 1104 70-71 -0.12884880459283465 1104 72-73 0.6338150442224304 1104 74-75 -0.690948744989722 1104 76 1.012158572373405 1105 1 -0.6400726401186709 1105 2 0.5190014979855917 1105 3 -0.059418663879160505 1105 4 0.4165555435645203 1105 5 0.46420487611846184 1105 6 0.5934957346451455 1105 7 1.4594433115694372 1105 8 0.0076505336199304 1105 9 1.2188020931112789 1105 10-11 1.1949453260477725 1105 12-13 -1.4560471474925194 1105 14-15 -0.21505589066845232 1105 16-17 0.5631434784823384 1105 18-19 0.6931789148199314 1105 20-21 -0.10312458753335818 1105 22-23 0.6625943451933196 1105 24-25 0.8053524118796247 1105 26-27 0.569167772215394 1105 28-29 -0.9250413792464443 1105 30-31 -0.24968945453743885 1105 32-33 0.8876480710565176 1105 34-35 0.8883062620504987 1105 36-37 0.7956692126454001 1105 38-39 0.8452112888843999 1105 40-41 0.8778996602600486 1105 42-43 1.0282277786112175 1105 44-45 1.893054237400662 1105 46-47 1.215074445919548 1105 48-49 0.9100734151422287 1105 50-51 0.19547753381394983 1105 52-53 -0.5313639490725919 1105 54-55 -0.33174250585270215 1105 56-57 0.05016457730641832 1105 58-59 1.010710560759236 1105 60-61 0.6243799142621214 1105 62-63 0.8644154217884648 1105 64-65 0.6845703844705398 1105 66-67 0.79079623890091 1105 68-69 1.9092622959631171 1105 70-71 1.806761017561172 1105 72-73 1.0574405746006974 1105 74-75 0.55233689191558 1105 76 -0.009101696652503932 1106 1 0.2030309821192482 1106 2 -0.049533463168682346 1106 3 -0.04829466024683171 1106 4 0.18950983677408928 1106 5 0.29752139311976933 1106 6 0.2913566972119881 1106 7 1.188633100692634 1106 8 1.259693717960566 1106 9 1.3067599177505684 1106 10-11 0.45364777786898003 1106 12-13 0.19373592182440547 1106 14-15 -0.0368574243179296 1106 16-17 0.1300766840115486 1106 18-19 0.0905782690727861 1106 20-21 -0.6216645935872336 1106 22-23 0.6438399309061609 1106 24-25 0.428170492799822 1106 26-27 -0.16113189482123147 1106 28-29 -1.2329694389780599 1106 30-31 -0.2560838647886712 1106 32-33 0.7169538928706629 1106 34-35 0.27064659097795385 1106 36-37 0.10099914907966934 1106 38-39 0.09914631057746703 1106 40-41 0.10697593914210302 1106 42-43 0.3777510355984006 1106 44-45 1.6642680402459789 1106 46-47 0.8956692395831638 1106 48-49 0.7207131084123333 1106 50-51 0.6181518350953539 1106 52-53 -0.2100038441386829 1106 54-55 0.14305822061283635 1106 56-57 1.1816094365537282 1106 58-59 -0.08456236830947716 1106 60-61 1.4893531762942018 1106 62-63 1.2027642261472664 1106 64-65 0.7748488629288204 1106 66-67 1.319590003404997 1106 68-69 1.8989454762678335 1106 70-71 1.3984520679092753 1106 72-73 1.3757232907746442 1106 74-75 -0.8909205997603351 1106 76 -0.38328190046645005 1107 1 0.28697761207635963 1107 2 0.25797964108291893 1107 3 0.8447005216242971 1107 4 0.3334712917431162 1107 5 0.10842307152290331 1107 6 0.49448583365504817 1107 7 0.8155987384328824 1107 8 1.4257338513856723 1107 9 0.2295904360965224 1107 10-11 1.0521282141478991 1107 12-13 1.212783414808996 1107 14-15 0.7152869077623336 1107 16-17 1.2280276555792966 1107 18-19 1.7393446162957389 1107 20-21 1.511297232007088 1107 22-23 0.6492139977246083 1107 24-25 0.8023167309530521 1107 26-27 0.6744825489760871 1107 28-29 0.012913972892540926 1107 30-31 0.7662595460229866 1107 32-33 1.3734504248768076 1107 34-35 1.3095887204850172 1107 36-37 0.7483359415824395 1107 38-39 1.6352822771152944 1107 40-41 1.0627259259054895 1107 42-43 1.2527047074361732 1107 44-45 0.607840338652295 1107 46-47 1.0547204388207305 1107 48-49 1.1241122718580954 1107 50-51 1.1524876216151725 1107 52-53 1.6594169214672903 1107 54-55 1.2000153869177055 1107 56-57 1.1622045548890867 1107 58-59 1.2769164266288442 1107 60-61 0.8422016964403412 1107 62-63 -0.16475046649847513 1107 64-65 0.7437428111471718 1107 66-67 2.0499733358558956 1107 68-69 1.9836598300561548 1107 70-71 0.9496248322445311 1107 72-73 1.0658937784116418 1107 74-75 0.6530514445029141 1107 76 -0.28726347509449823 1108 1 -0.2744479334632679 1108 2 0.7772151467230373 1108 3 0.14453796805550567 1108 4 0.26450917165050924 1108 5 0.3559724533267534 1108 6 0.1482221028043469 1108 7 0.7034121466393728 1108 8 1.0608749774385728 1108 9 -0.0011124849418493454 1108 10-11 0.6629355635395626 1108 12-13 0.6323317129223867 1108 14-15 1.384059382024688 1108 16-17 -0.44139749589107424 1108 18-19 0.13923871018274525 1108 20-21 0.41266908561036075 1108 22-23 -0.23951069564024863 1108 24-25 -0.3970084254431896 1108 26-27 -0.22112604173372574 1108 28-29 0.9185590150086895 1108 30-31 0.8443098883706597 1108 32-33 0.7184703746329788 1108 34-35 0.600042392717917 1108 36-37 0.6766769100076431 1108 38-39 1.2822947395555673 1108 40-41 1.2463991439735622 1108 42-43 1.1325997864963213 1108 44-45 0.1247730020678901 1108 46-47 0.8629318569774753 1108 48-49 -0.2105264158912732 1108 50-51 0.5511871930648553 1108 52-53 0.8972007744794581 1108 54-55 1.4508774134188727 1108 56-57 1.5949172291415863 1108 58-59 -0.4947573825431206 1108 60-61 1.1305988197347574 1108 62-63 0.4053076587170352 1108 64-65 1.2713769178414225 1108 66-67 0.2653691291665652 1108 68-69 1.868123649572432 1108 70-71 2.3731507669275516 1108 72-73 0.9481286436892162 1108 74-75 1.812181536616599 1108 76 0.5843930935474368 1109 1 -0.8320049758376022 1109 2 -1.2561402438119735 1109 3 -0.033463649472615487 1109 4 0.3623451150860362 1109 5 0.9076475752540638 1109 6 0.9358602666648466 1109 7 -0.8467189711705885 1109 8 0.9741901748505271 1109 9 1.3693504712133375 1109 10-11 1.164087439627096 1109 12-13 0.5461370703715929 1109 14-15 0.5851230293055281 1109 16-17 -0.013083401339201828 1109 18-19 0.7005332228411234 1109 20-21 1.0473773178481807 1109 22-23 0.7828773640612425 1109 24-25 1.2730771074833171 1109 26-27 0.4775235673636402 1109 28-29 0.1556004090238403 1109 30-31 0.4845446750285092 1109 32-33 1.435770573147792 1109 34-35 0.19933656226427132 1109 36-37 0.42766335708261494 1109 38-39 0.9675507256350215 1109 40-41 0.3365273208233006 1109 42-43 0.40857214147021637 1109 44-45 0.16496612512256803 1109 46-47 -0.01153815748224929 1109 48-49 -0.07163956933587912 1109 50-51 0.9399821346132811 1109 52-53 0.7786555694700183 1109 54-55 0.6338909173278964 1109 56-57 0.4110949611710879 1109 58-59 0.14443581403556038 1109 60-61 0.928567106380342 1109 62-63 -0.3917538318596385 1109 64-65 -0.1826338166633903 1109 66-67 0.1938425257305454 1109 68-69 1.5512596934907705 1109 70-71 0.4908626998736594 1109 72-73 0.20220309899887212 1109 74-75 1.793518693198223 1109 76 -0.34737662926706037 1110 1 0.9891272388037393 1110 2 0.6830868576869342 1110 3 0.1495663023200109 1110 4 0.8151782859719461 1110 5 0.15313636638180128 1110 6 0.39296097528685436 1110 7 0.9105582343824778 1110 8 2.6981963917573566 1110 9 -0.44190318385370375 1110 10-11 -0.30838430769252057 1110 12-13 -0.20198600330928684 1110 14-15 0.04603027621682543 1110 16-17 0.2637665235837474 1110 18-19 0.9114338840460405 1110 20-21 -0.24465924599365252 1110 22-23 0.4887597170024165 1110 24-25 0.775764075687519 1110 26-27 -0.6531105044469001 1110 28-29 0.7483139834818289 1110 30-31 0.1743974186337809 1110 32-33 1.4148148554375126 1110 34-35 1.259951403812174 1110 36-37 0.5769349779566681 1110 38-39 0.7167933694009889 1110 40-41 1.0347834257731208 1110 42-43 1.086307185331016 1110 44-45 1.4610594840962463 1110 46-47 0.614920576481552 1110 48-49 1.0843494720487072 1110 50-51 0.6716277709242249 1110 52-53 0.17769669062067805 1110 54-55 0.052149129703749963 1110 56-57 0.5666361745216335 1110 58-59 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42-43 0.5812938163470633 2116 44-45 1.8695535635462122 2116 46-47 0.934582532172918 2116 48-49 1.2257742237675657 2116 50-51 0.6299451888004697 2116 52-53 1.766648180307456 2116 54-55 0.3928194887564658 2116 56-57 0.3387427444223263 2116 58-59 0.05745921305033619 2116 60-61 -0.8399058000235939 2116 62-63 0.566066365184696 2116 64-65 1.7383945084597556 2116 66-67 0.041133166124630804 2116 68-69 0.36268210728387373 2116 70-71 -1.9603380390425968 2116 72-73 0.5268644290481319 2116 74-75 -1.5017399274914318 2116 76 -1.7402337721242027 2117 1 -0.6317938569072723 2117 2 -1.2818263803355485 2117 3 -0.20834049676325606 2117 4 -0.978611957398126 2117 5 -1.9143087935003322 2117 6 -1.7840552857630172 2117 7 -3.206324882500642 2117 8 -3.1662039138194196 2117 9 -0.7460612107008089 2117 10-11 -1.5517816666360886 2117 12-13 -0.9072408325137005 2117 14-15 -2.5855314393784994 2117 16-17 -1.0185373070394164 2117 18-19 -1.4301845815219991 2117 20-21 -1.8277106483727863 2117 22-23 -1.0334491665510015 2117 24-25 -0.5057758129952248 2117 26-27 -0.05308867753431912 2117 28-29 -0.2666374516376706 2117 30-31 -0.600430694429626 2117 32-33 0.5244405238867103 2117 34-35 -1.3879259289391044 2117 36-37 -1.7359538139237145 2117 38-39 -0.3887124692981274 2117 40-41 -1.8759990690429866 2117 42-43 -2.45314495916314 2117 44-45 -0.6374617425762352 2117 46-47 -1.4008766515005462 2117 48-49 -2.2971849599058984 2117 50-51 0.814894168392307 2117 52-53 0.130168588470724 2117 54-55 -1.7283539806312902 2117 56-57 -2.879369500475633 2117 58-59 -1.9074642563374127 2117 60-61 -0.18174253471747193 2117 62-63 -1.5984744511418327 2117 64-65 -1.203569777254529 2117 66-67 -2.91294846652843 2117 68-69 -1.854792382512045 2117 70-71 -1.0821492635323935 2117 72-73 0.2775021841501726 2117 74-75 -1.3933215601444928 2117 76 -2.8881929557976704 2118 1 -0.08239682536738258 2118 2 1.0961884619650064 2118 3 -0.3544443928671548 2118 4 0.43349379399334254 2118 5 0.044179147130464 2118 6 0.26395028009969224 2118 7 1.2741945980188376 2118 8 -4.1126324852479925 2118 9 -1.2513951624633322 2118 10-11 -1.566739922665775 2118 12-13 0.27562094856236996 2118 14-15 -1.1284349644248834 2118 16-17 -0.11524417160342182 2118 18-19 -1.0427773459004825 2118 20-21 -1.9996726149776123 2118 22-23 -0.07507718139330422 2118 24-25 -0.44582683340338747 2118 26-27 0.9361274634675354 2118 28-29 -0.35128494699944923 2118 30-31 -0.9729956294945552 2118 32-33 -0.617604930658743 2118 34-35 1.1897642380367799 2118 36-37 -0.17774416642835433 2118 38-39 0.1994136902566055 2118 40-41 -1.4237727239595017 2118 42-43 -1.0096152745620302 2118 44-45 0.4600568102994558 2118 46-47 -0.6741837015933143 2118 48-49 -1.6362387632454158 2118 50-51 -0.11112623977096092 2118 52-53 -1.629455715796432 2118 54-55 -0.7402973943604181 2118 56-57 1.1358206665002513 2118 58-59 0.5992611611022909 2118 60-61 -2.0688019039196988 2118 62-63 0.27629363791196937 2118 64-65 -0.9641217253064767 2118 66-67 -2.8525356650442006 2118 68-69 1.0132502891020536 2118 70-71 -1.0199971299516903 2118 72-73 -1.9451323241986174 2118 74-75 -0.8548243430758475 2118 76 -0.08114286303329266 2119 1 -1.214313821414816 2119 2 0.34091836973839307 2119 3 0.707808571559724 2119 4 0.9629733723859601 2119 5 0.3944755898182102 2119 6 1.0210319665292005 2119 7 0.191190645449673 2119 8 1.7623675147520075 2119 9 0.7747906082876526 2119 10-11 0.7798509864251315 2119 12-13 1.405314624451698 2119 14-15 -0.55580650592686 2119 16-17 0.9998744054716795 2119 18-19 0.13599406384918922 2119 20-21 -0.5992279509459948 2119 22-23 0.7466454800032736 2119 24-25 0.42464418108936286 2119 26-27 0.3077513106348633 2119 28-29 1.8115938408793326 2119 30-31 0.7013291399389061 2119 32-33 0.37226661084322643 2119 34-35 0.9428933552963272 2119 36-37 0.36919259246492686 2119 38-39 0.8683860222724178 2119 40-41 0.0646989492947796 2119 42-43 -0.3163511111891708 2119 44-45 -0.9695380513606224 2119 46-47 0.4230659690259202 2119 48-49 -0.6569897513877052 2119 50-51 0.767496948634836 2119 52-53 -0.8597752151376739 2119 54-55 -0.824701215177285 2119 56-57 1.1000005083974784 2119 58-59 2.2311781571497136 2119 60-61 0.2439451316534189 2119 62-63 0.5071895535904929 2119 64-65 1.1747946119794292 2119 66-67 0.6104913441784632 2119 68-69 -0.19936499416539988 2119 70-71 0.7267090624066839 2119 72-73 -2.7343286351340623 2119 74-75 0.3490623499412848 2119 76 -0.41777000400826125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 14.0 13 67.0 14 225.0 15 420.0 16 524.0 17 580.0 18 588.0 19 533.0 20 465.0 21 436.0 22 391.0 23 492.0 24 493.0 25 647.0 26 758.0 27 871.0 28 1117.0 29 1407.0 30 1778.0 31 2247.0 32 3021.0 33 4008.0 34 5714.0 35 8466.0 36 13228.0 37 10909.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.02622484244765 26.28583045334418 11.49115673917463 28.196787965033543 2 17.25728523088837 20.743758522583793 36.0162286830188 25.982727563509034 3 17.5737782192219 25.745357822258885 29.46415043517786 27.216713523341358 4 12.624366593154997 19.306072288345312 39.2047271931449 28.864833925354795 5 13.38361307048703 36.426996178515516 35.85293176882544 14.336458982172019 6 25.94064072995404 37.98252554670797 18.127640948805578 17.949192774532417 7 25.105637952223027 29.930472550967156 24.571976902745746 20.391912594064074 8 21.671352334135786 34.49100183498594 19.524923822831266 24.312722008047004 9 23.23193212235484 15.452601808050371 22.506355111866803 38.80911095772798 10-11 23.64522482786485 25.75966734566758 27.60138718203397 22.993720644433598 12-13 23.161226241982458 23.703304658170737 26.587936230029797 26.547532869817008 14-15 21.139374758000706 25.438965673978554 24.239490917661318 29.18216865035942 16-17 22.02740694601101 27.587077658625276 26.38423595562364 24.001279439740074 18-19 21.437349539570043 27.050891399134695 26.75628356424976 24.755475497045502 20-21 20.76480193936129 27.43977374118281 26.749549670880963 25.04587464857494 22-23 22.509722058551205 26.83961549468864 26.65780037373108 23.992862073029073 24-25 21.599804717092304 27.908621066985408 26.612346593491694 23.879227622430598 26-27 20.025757142135657 28.542448780323564 27.617380178784867 23.814413898755912 28-29 21.75552600124577 27.25627514688305 28.513829733506167 22.47436911836501 30-31 21.651150654029394 27.405262537667717 27.84380734331072 23.09977946499217 32-33 20.34814228716688 28.216696688607932 27.55593340179458 23.879227622430598 34-35 21.511422366626824 27.86905944344371 27.619905388798166 22.999612801131295 36-37 21.865793505159843 27.305937610477937 27.400212117641114 23.4280567667211 38-39 21.196612851635493 27.925455800407402 27.898520226932206 22.9794111210249 40-41 21.585495193683606 27.012171512264104 27.952391373882595 23.449941920169696 42-43 20.76311846601909 27.149374589653373 28.227639265332233 23.859867678995304 44-45 20.273227723439 26.718405414050267 28.440598643120484 24.567768219390246 46-47 20.738708102557197 26.65864211040218 28.61147118735375 23.991178599686876 48-49 19.985353781922864 26.864025858150537 29.209104223834615 23.941516136091984 50-51 20.160435009511623 26.747024460867664 28.679651857712834 24.412888671907883 52-53 19.85067591454689 26.707462837325973 28.59968687395835 24.842174374168785 54-55 19.211797781182135 27.66030874901096 28.715004797899024 24.412888671907883 56-57 19.45590141580108 27.12075554283598 28.70742916785913 24.715913873503816 58-59 19.87256106799549 26.83540681133314 28.316021615797716 24.976010504873656 60-61 19.842258547835893 26.465042676049226 28.07360145452097 25.619097321593916 62-63 19.471052675880877 26.66453426709988 28.369051026077003 25.495362030942243 64-65 20.418006430868168 26.53659029309271 27.4902779414488 25.55512533459033 66-67 20.32036497702059 26.62328917021599 26.96335078534031 26.092995067423107 68-69 19.46431878251208 26.858975438123938 27.325297553913234 26.35140822545075 70-71 20.735341155872796 26.785744347738255 26.87665190821703 25.602262588171914 72-73 20.85655123651117 26.69315331391727 25.925489469874247 26.524805979697312 74-75 20.80099661621858 26.547532869817008 26.17296005117759 26.47851046278682 76 21.147792124711707 26.004612716957627 26.124139324253797 26.723455834076866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 1.5 17 2.0 18 3.0 19 4.0 20 19.5 21 35.0 22 29.5 23 33.0 24 65.5 25 89.0 26 119.5 27 181.5 28 258.0 29 303.0 30 381.5 31 525.0 32 775.5 33 961.0 34 1084.0 35 1380.5 36 1721.5 37 1889.0 38 2129.0 39 2723.5 40 3298.5 41 3737.5 42 3956.0 43 3970.0 44 4224.0 45 4459.5 46 4455.0 47 4388.0 48 4233.0 49 3849.0 50 3553.0 51 3316.5 52 2844.5 53 2355.5 54 2102.0 55 1914.0 56 1512.5 57 1162.0 58 1025.0 59 857.5 60 564.0 61 381.5 62 325.0 63 259.0 64 168.0 65 118.0 66 83.0 67 73.0 68 66.5 69 48.0 70 32.5 71 29.0 72 25.5 73 21.5 74 16.0 75 11.0 76 12.0 77 9.5 78 6.5 79 7.0 80 6.0 81 3.5 82 2.0 83 2.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 2.0 90 3.0 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 1.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6279355566404606 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59401.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.665662194239154 #Duplication Level Percentage of deduplicated Percentage of total 1 76.28519835223746 45.5160687530513 2 10.42548388917104 12.44086799885524 3 4.850177755205689 8.681672025723474 4 2.7650809773714804 6.5992155014225355 5 1.6985497432424808 5.067254760020875 6 0.9875289204898144 3.535294018619215 7 0.7984876699960499 3.3349606908974594 8 0.5163365498561029 2.464604972980253 9 0.44579876982111616 2.3938990926078687 >10 1.2273573726087692 9.966162185821787 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016834733421996263 0.0 2 0.0 0.0 0.0 0.006733893368798505 0.0 3 0.0 0.0 0.0 0.006733893368798505 0.0 4 0.0 0.0 0.0 0.006733893368798505 0.0 5 0.0 0.0 0.0 0.006733893368798505 0.0 6 0.0 0.0 0.0 0.006733893368798505 0.0 7 0.0 0.0 0.0 0.006733893368798505 0.0 8 0.0 0.0 0.0 0.010100840053197757 0.0 9 0.0 0.0 0.0 0.015151260079796636 0.0 10 0.0 0.0 0.0 0.02188515344859514 0.0 11 0.0 0.0 0.0 0.025252100132994393 0.0 12 0.0 0.0 0.0 0.033669466843992524 0.0 13 0.0 0.0 0.0 0.038719886870591406 0.0 14 0.0 0.0 0.0 0.04377030689719028 0.0 15 0.0 0.0 0.0 0.048820726923789165 0.0 16 0.0 0.0 0.0 0.050504200265988786 0.0 17 0.0 0.0 0.0 0.050504200265988786 0.0 18 0.0 0.0 0.0 0.060605040319186544 0.0 19 0.0 0.0 0.0 0.06228851366138617 0.0 20 0.0 0.0 0.0 0.06228851366138617 0.0 21 0.0 0.0 0.0 0.06565546034578543 0.0 22 0.0 0.0 0.0 0.0707058803723843 0.0 23 0.0 0.0 0.0 0.07238935371458392 0.0 24 0.0 0.0 0.0 0.07238935371458392 0.0 25 0.0 0.0 0.0 0.07575630039898318 0.0 26 0.0 0.0 0.0 0.08417366710998131 0.0 27 0.0 0.0 0.0 0.10100840053197757 0.0 28 0.0 0.0 0.0 0.10437534721637683 0.0 29 0.0 0.0 0.0 0.11279271392737496 0.0 30 0.0 0.0 0.0 0.13636134071816972 0.0 31 0.0 0.0 0.0 0.14814565411356712 0.0 32 0.0 0.0 0.0 0.17003080756216224 0.0 33 0.0 0.0 0.0 0.18686554098415853 0.0 34 0.0 0.0 0.0 0.22221848117035067 0.0 35 0.0 0.0 0.0 0.27608962812073873 0.0 36 0.0 0.0 0.0 0.37541455531051665 0.0 37 0.0 0.0 0.0 0.5201932627396845 0.0 38 0.0 0.0 0.0 0.8400531977576136 0.0 39 0.0 0.0 0.0 1.2171512264103297 0.0 40 0.0 0.0 0.0 1.6211848285382402 0.0 41 0.0 0.0 0.0 2.164946718068719 0.0 42 0.0 0.0 0.0 2.6868234541506033 0.0 43 0.0 0.0 0.0 3.291190384000269 0.0 44 0.0 0.0 0.0 3.8265349068197505 0.0 45 0.0 0.0 0.0 4.360195956297032 0.0 46 0.0 0.0 0.0 5.016750559754886 0.0 47 0.0 0.0 0.0 5.67498863655494 0.0 48 0.0 0.0 0.0 6.277672093062407 0.0 49 0.0 0.0 0.0 6.797865355802091 0.0 50 0.0 0.0 0.0 7.38876449891416 0.0 51 0.0 0.0 0.0 7.998181848790424 0.0 52 0.0 0.0 0.0 8.69513981246107 0.0 53 0.0 0.0 0.0 9.4628036565041 0.0 54 0.0 0.0 0.0 10.110940893250955 0.0 55 0.0 0.0 0.0 10.663120149492432 0.0 56 0.0 0.0 0.0 11.2624366593155 0.0 57 0.0 0.0 0.0 11.777579502028585 0.0 58 0.0 0.0 0.0 12.269153717950877 0.0 59 0.0 0.0 0.0 12.804498240770357 0.0 60 0.0 0.0 0.0 13.412232117304422 0.0 61 0.0 0.0 0.0 13.91559064662211 0.0 62 0.0 0.0 0.0 14.533425363209373 0.0 63 0.0 0.0 0.0 15.18156259995623 0.0 64 0.0 0.0 0.0 15.752260062961904 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTTT 15 0.0022154546 69.992424 26 TCGTTCG 15 0.0022154546 69.992424 23 AGGACGG 15 0.0022154546 69.992424 28 CGTTCGG 15 0.0022154546 69.992424 24 TCTAACT 15 0.0022154546 69.992424 51 GGTATTG 15 0.0022154546 69.992424 56 AGGGGCG 15 0.0022154546 69.992424 46 GTGAAAC 15 0.0022154546 69.992424 41 TCGGTTC 15 0.0022154546 69.992424 13 GGGGCGT 15 0.0022154546 69.992424 47 CAGTCTA 25 2.3738304E-4 55.993935 19 TAGAGTA 20 0.0069227507 52.494316 26 CGGTTCA 20 0.0069227507 52.494316 14 AAGCATT 20 0.0069227507 52.494316 32 CTGCACA 20 0.0069227507 52.494316 40 TTCGGTA 20 0.0069227507 52.494316 26 TAGGACG 20 0.0069227507 52.494316 27 CGTTTGG 20 0.0069227507 52.494316 51 TGGCTGC 20 0.0069227507 52.494316 67 TCGGTAA 20 0.0069227507 52.494316 27 >>END_MODULE