FastQCFastQC Report
Wed 25 May 2016
SRR1780736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4060.6834901769330483TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3330.5605966229524756No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1210.20370027440615476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA150.002214503270.0000162
CAACGGA150.002214503270.0000159
CTATCGG150.002214503270.0000139
AACGGAA150.002214503270.0000160
ACGGAAC150.002214503270.0000161
TCCAATA150.002214503270.0000145
ACCAACG150.002214503270.0000157
TAAAGAA207.866982E-570.02
CATGGTT252.3725611E-455.99999614
ACACGTA252.3725611E-455.99999638
ATGATTC252.3725611E-455.99999640
AATTTAC252.3725611E-455.99999653
TGAAGGT252.3725611E-455.99999628
TTGAGGC252.3725611E-455.99999611
ATTGGCG252.3725611E-455.99999621
GCGTGAA252.3725611E-455.99999625
TTGCACC252.3725611E-455.99999638
ATAAAGA252.3725611E-455.9999961
GCATGGT252.3725611E-455.99999613
CGCCATT252.3725611E-455.99999617
Produced by FastQC (version 0.11.5)