##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59401 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32839514486288 34.0 34.0 34.0 27.0 34.0 2 33.09073921314456 34.0 34.0 34.0 32.0 34.0 3 33.31315971111597 34.0 34.0 34.0 33.0 34.0 4 33.25925489469874 34.0 34.0 34.0 33.0 34.0 5 33.284624837965694 34.0 34.0 34.0 33.0 34.0 6 36.95575832056699 38.0 37.0 38.0 35.0 38.0 7 36.59938384875675 38.0 37.0 38.0 34.0 38.0 8 36.49026110671537 38.0 37.0 38.0 34.0 38.0 9 36.3434117270753 38.0 37.0 38.0 34.0 38.0 10-11 35.950085015403786 38.0 36.5 38.0 32.5 38.0 12-13 35.84970791737513 38.0 36.0 38.0 31.0 38.0 14-15 36.14249760105049 38.0 36.5 38.0 33.0 38.0 16-17 36.31970000505042 38.0 37.0 38.0 34.0 38.0 18-19 36.3153229743607 38.0 37.0 38.0 34.0 38.0 20-21 36.01373714247235 38.0 36.5 38.0 32.5 38.0 22-23 36.00325752091716 38.0 36.5 38.0 32.5 38.0 24-25 35.171411255702765 37.5 35.0 38.0 29.0 38.0 26-27 35.49301358562987 37.5 36.0 38.0 31.0 38.0 28-29 35.28586219087221 37.5 36.0 38.0 29.0 38.0 30-31 34.90181141731621 37.0 35.5 38.0 27.0 38.0 32-33 35.465303614417266 37.5 36.0 38.0 31.0 38.0 34-35 35.55716233733439 38.0 36.0 38.0 31.5 38.0 36-37 35.78640090234171 38.0 36.5 38.0 32.0 38.0 38-39 35.47989932829414 38.0 36.5 38.0 29.5 38.0 40-41 34.77428831164458 37.0 34.5 38.0 27.0 38.0 42-43 35.26016397030353 37.0 35.5 38.0 29.0 38.0 44-45 35.4777276476827 37.5 36.0 38.0 31.0 38.0 46-47 35.76588777966701 38.0 36.5 38.0 31.5 38.0 48-49 35.514999747478996 38.0 36.0 38.0 31.0 38.0 50-51 35.732487668557766 38.0 36.0 38.0 31.5 38.0 52-53 35.63345734920287 38.0 36.0 38.0 31.0 38.0 54-55 35.82264608339927 38.0 36.5 38.0 31.5 38.0 56-57 35.92268648675948 38.0 37.0 38.0 33.0 38.0 58-59 36.01482298277807 38.0 37.0 38.0 33.0 38.0 60-61 35.58769212639518 38.0 36.5 38.0 29.5 38.0 62-63 35.90052356020942 38.0 36.5 38.0 32.5 38.0 64-65 35.92397434386626 38.0 37.0 38.0 33.0 38.0 66-67 35.1915624316089 38.0 36.0 38.0 29.0 38.0 68-69 34.559443443713064 37.0 34.5 38.0 26.0 38.0 70-71 34.54899749162472 37.0 34.0 38.0 25.5 38.0 72-73 34.21373377552567 37.0 34.0 38.0 24.5 38.0 74-75 35.038770391070855 37.0 35.5 38.0 28.5 38.0 76 34.429151024393526 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9126841680922411 1101 2 0.7360621947524919 1101 3 0.3544873575993037 1101 4 0.6254527297325723 1101 5 0.4377466499571341 1101 6 -0.4671803444969811 1101 7 0.45923787857326204 1101 8 1.0246523612537146 1101 9 0.5491395710935549 1101 10-11 0.5685601815116144 1101 12-13 1.0592631934743295 1101 14-15 1.105174620676486 1101 16-17 0.27912008685765244 1101 18-19 0.2541880698432166 1101 20-21 0.3940545421989583 1101 22-23 1.0505735709722046 1101 24-25 -1.446232944412749 1101 26-27 0.15934104574106556 1101 28-29 1.2728049651098843 1101 30-31 1.681810831560476 1101 32-33 0.8705712644965544 1101 34-35 1.0337782437015335 1101 36-37 1.16430961257619 1101 38-39 0.8536814371450419 1101 40-41 1.626858215658018 1101 42-43 1.4061919869999642 1101 44-45 1.064369155439671 1101 46-47 1.1755266853363224 1101 48-49 -1.2877564760104576 1101 50-51 -0.9231267970643842 1101 52-53 -1.5927912415556804 1101 54-55 -0.4073213226279222 1101 56-57 -0.8666028771547687 1101 58-59 -0.7175883540326922 1101 60-61 -1.2538559772270617 1101 62-63 -0.4246277688999385 1101 64-65 -0.5581566289477351 1101 66-67 -0.9287198799991643 1101 68-69 0.13092277538824248 1101 70-71 0.28410564803449745 1101 72-73 -1.3557353125335254 1101 74-75 -0.3706230863135218 1101 76 -0.13567777922914104 1102 1 -1.0484649002307407 1102 2 0.31134169785808297 1102 3 0.14383518368625658 1102 4 0.5283409284903371 1102 5 0.33842987977079986 1102 6 0.430055680347742 1102 7 0.14193974192730252 1102 8 0.9278821749183166 1102 9 0.6246985773047342 1102 10-11 0.8737465169153396 1102 12-13 1.0668874170768134 1102 14-15 0.09463114241561499 1102 16-17 0.4864182235036125 1102 18-19 -0.33317217860398074 1102 20-21 0.5629831136275314 1102 22-23 0.6300145647610265 1102 24-25 -4.4008292177046755 1102 26-27 -0.8256278983583201 1102 28-29 0.3139229775322434 1102 30-31 1.560708347088422 1102 32-33 0.9712234384096021 1102 34-35 1.250812405192221 1102 36-37 -2.234075480591521 1102 38-39 0.09667833155498329 1102 40-41 1.2333488988878258 1102 42-43 0.9417354652608338 1102 44-45 0.9192604597875018 1102 46-47 1.3207440766406648 1102 48-49 -3.609899333153315 1102 50-51 0.187509848898344 1102 52-53 -0.0047011794438844845 1102 54-55 0.18739917426648844 1102 56-57 0.8349188619756731 1102 58-59 0.25214767081202893 1102 60-61 -5.7398342380966305 1102 62-63 -2.1493172098937308 1102 64-65 0.03516635241872024 1102 66-67 1.1205813622368552 1102 68-69 -0.7537666656055535 1102 70-71 -1.1107856563133254 1102 72-73 -6.222567610670168 1102 74-75 -2.5189926515309153 1102 76 -1.0676964127695143 1103 1 1.116272207959348 1103 2 0.8301485734900353 1103 3 0.32378968318069923 1103 4 0.5186088759119798 1103 5 0.49409216490730046 1103 6 0.6659758016824213 1103 7 1.3278093071446904 1103 8 1.1738882416523921 1103 9 0.6163820960104971 1103 10-11 0.5764007130730775 1103 12-13 -0.1293082350971062 1103 14-15 -0.23156956537002316 1103 16-17 0.4389207513094746 1103 18-19 1.2407661429329195 1103 20-21 -0.06056339800037591 1103 22-23 1.2051915111715417 1103 24-25 -2.3639738082001287 1103 26-27 0.09894237464471445 1103 28-29 0.6426388521530697 1103 30-31 1.673638074085396 1103 32-33 1.7040596365895837 1103 34-35 0.9476652868909028 1103 36-37 -0.41921198210820165 1103 38-39 0.387767815882583 1103 40-41 0.1365592123357544 1103 42-43 0.2661919869999636 1103 44-45 1.8806482252071177 1103 46-47 1.4648290109177111 1103 48-49 -1.79187607733936 1103 50-51 0.23567718964658013 1103 52-53 1.0438200541253835 1103 54-55 0.25580160096011895 1103 56-57 0.6743273554033706 1103 58-59 0.14068407121647653 1103 60-61 -3.92222807025032 1103 62-63 -0.8821028519564251 1103 64-65 0.4787204474642195 1103 66-67 0.33373859176163023 1103 68-69 0.9692616458201258 1103 70-71 1.2929428573368256 1103 72-73 -4.153609066686343 1103 74-75 -1.4869021560809657 1103 76 -2.613019971920174 1104 1 -0.0442262898708492 1104 2 0.5924941838160578 1104 3 0.6714251566423641 1104 4 -0.033521979768451615 1104 5 -0.13020960431491346 1104 6 0.26121336705462284 1104 7 0.9153691157571302 1104 8 1.0084937829898735 1104 9 1.3155920659671025 1104 10-11 -0.30185676995796484 1104 12-13 0.7802611428933304 1104 14-15 1.1509162474707537 1104 16-17 0.5634673185733021 1104 18-19 0.9407772701166337 1104 20-21 0.0752438791777692 1104 22-23 0.5712981095914813 1104 24-25 1.043158716557862 1104 26-27 1.5341599110862418 1104 28-29 1.8787243089239638 1104 30-31 1.1264588151558286 1104 32-33 0.9711454271759763 1104 34-35 0.6359928711793188 1104 36-37 1.6399623808605241 1104 38-39 1.6759507467827746 1104 40-41 2.153283369451593 1104 42-43 1.0618857669042754 1104 44-45 1.1495366195545031 1104 46-47 1.4014596996903919 1104 48-49 0.43136860943041455 1104 50-51 0.9863058755261846 1104 52-53 1.0319250947395986 1104 54-55 0.47682085098794147 1104 56-57 0.5141626730844706 1104 58-59 -0.7698644989404144 1104 60-61 0.6101631615289165 1104 62-63 0.7159053821595265 1104 64-65 0.7782753601158348 1104 66-67 0.7278078028443105 1104 68-69 2.2095420508496204 1104 70-71 2.2761630643024517 1104 72-73 1.817894215832851 1104 74-75 0.5059319376099225 1104 76 -0.2631145529817047 1105 1 1.400366108793051 1105 2 0.753097235183759 1105 3 0.6531666028823224 1105 4 -0.027134871345595002 1105 5 0.061358509795411464 1105 6 0.30608111102862523 1105 7 1.0466772316729944 1105 8 -0.45480855518832897 1105 9 0.9067945576164647 1105 10-11 0.8545709631827734 1105 12-13 0.25654082150666824 1105 14-15 0.6244199036953617 1105 16-17 0.6524354507390555 1105 18-19 0.9867621938324405 1105 20-21 0.7764265098539411 1105 22-23 0.9564091504870333 1105 24-25 1.4227967051829395 1105 26-27 0.5599340376548056 1105 28-29 1.5927375796651404 1105 30-31 1.8980237695658673 1105 32-33 1.5326467361946712 1105 34-35 -0.9205883331178768 1105 36-37 0.7986357581287464 1105 38-39 0.6512825153121611 1105 40-41 1.3252274878952122 1105 42-43 0.9874184020943062 1105 44-45 1.35399179694911 1105 46-47 1.1305266853363207 1105 48-49 -0.23489933315331513 1105 50-51 1.1304446315070464 1105 52-53 1.0084756048055041 1105 54-55 1.5286625048653377 1105 56-57 1.0411329719018383 1105 58-59 0.9194466863985724 1105 60-61 1.1420874189993455 1105 62-63 -0.15454690636624946 1105 64-65 1.1511425624269265 1105 66-67 0.5834095000547492 1105 68-69 0.6608149586766459 1105 70-71 1.6148603650156375 1105 72-73 1.4372970324799539 1105 74-75 0.17465993255439827 1105 76 1.1986079350565717 1106 1 -1.0416932192263246 1106 2 0.07011872645608008 1106 3 0.20111837364208185 1106 4 0.15223653416343552 1106 5 0.12028293719930616 1106 6 0.012972443891101193 1106 7 -0.5310142222670748 1106 8 0.5750572504821392 1106 9 -0.27151741897520054 1106 10-11 0.8504089210074142 1106 12-13 1.650370909287922 1106 14-15 -0.4853066627459768 1106 16-17 0.3184630967888964 1106 18-19 0.8811093838233646 1106 20-21 0.5265526323441136 1106 22-23 0.6699471385810654 1106 24-25 -0.016790622029255076 1106 26-27 1.1238024666730695 1106 28-29 0.8276407175926934 1106 30-31 1.0247138109340455 1106 32-33 0.45003650513826443 1106 34-35 0.9575521168871717 1106 36-37 1.02667783259146 1106 38-39 0.4015347602924777 1106 40-41 0.9243219284158499 1106 42-43 1.2308978693529085 1106 44-45 1.2376311875252313 1106 46-47 1.1084678618069077 1106 48-49 1.5552076187183488 1106 50-51 0.20597939086533756 1106 52-53 0.7955051679171916 1106 54-55 0.5930606483422807 1106 56-57 0.6437714543960382 1106 58-59 1.027912027938278 1106 60-61 0.9891386751793547 1106 62-63 1.0996655848051802 1106 64-65 -0.41789688868799857 1106 66-67 0.32223504742635356 1106 68-69 1.3962856478099326 1106 70-71 0.7767259688901191 1106 72-73 0.21826010381484195 1106 74-75 -0.4342594499498844 1106 76 -0.25326372269744013 1107 1 1.122811466536369 1107 2 -0.2364413413153983 1107 3 0.10062597146068697 1107 4 0.429384837229037 1107 5 0.31486121855684956 1107 6 0.6975438422074021 1107 7 1.0624627724912301 1107 8 0.681003139188654 1107 9 -0.7146793822303508 1107 10-11 0.8134258109316974 1107 12-13 0.39197889361577154 1107 14-15 0.6704221454289652 1107 16-17 0.9470833117515625 1107 18-19 0.9857580839874984 1107 20-21 0.9571662819443603 1107 22-23 0.15874895993967186 1107 24-25 1.0813059523340698 1107 26-27 0.4479407628556302 1107 28-29 1.414870028759104 1107 30-31 1.1949367155774482 1107 32-33 1.7594821555856655 1107 34-35 0.6565646652008255 1107 36-37 1.6796137144149412 1107 38-39 0.7305838416712049 1107 40-41 0.7099416668602743 1107 42-43 1.0255979275940277 1107 44-45 1.3807057891030396 1107 46-47 1.1515662892967171 1107 48-49 1.074506607440746 1107 50-51 1.386632750318924 1107 52-53 1.1182271459973592 1107 54-55 0.5817168669053174 1107 56-57 1.3152783109640538 1107 58-59 1.0437694960380313 1107 60-61 1.6458469930699664 1107 62-63 1.4143396992754518 1107 64-65 1.0508249834992185 1107 66-67 0.54263797007156 1107 68-69 1.478207075246793 1107 70-71 0.432719509420636 1107 72-73 2.0056932588950502 1107 74-75 -0.15478150215511022 1107 76 1.3867267469377609 1108 1 -0.8552191723484128 1108 2 0.40943817200906807 1108 3 -0.07158229414199013 1108 4 0.5919630353473551 1108 5 0.2779172256500999 1108 6 -0.2795755115190133 1108 7 -0.01945437444734921 1108 8 -0.4728316472750649 1108 9 -0.08022787737836978 1108 10-11 0.8568473172614404 1108 12-13 1.104025098236228 1108 14-15 0.5397019838605743 1108 16-17 -0.2887548243008453 1108 18-19 0.4117715808595719 1108 20-21 1.202597541488224 1108 22-23 0.5843191288968654 1108 24-25 0.9107109077549822 1108 26-27 -0.16188141339184625 1108 28-29 0.13665713284264314 1108 30-31 0.4837155934652344 1108 32-33 1.065994150068697 1108 34-35 0.8962942351727534 1108 36-37 1.5145725834361627 1108 38-39 1.0794952253468892 1108 40-41 1.0826080379750067 1108 42-43 0.24479895217410075 1108 44-45 0.8986975136486279 1108 46-47 0.6236858893164197 1108 48-49 1.2066429554039004 1108 50-51 0.8188774673279369 1108 52-53 0.28030044287928746 1108 54-55 -0.342289339685486 1108 56-57 0.6961831924969815 1108 58-59 0.6031508073034857 1108 60-61 1.0974873063550277 1108 62-63 1.2636451522088024 1108 64-65 0.9335278060202796 1108 66-67 1.2077406743718413 1108 68-69 1.6840713183875238 1108 70-71 -0.18475007335852922 1108 72-73 0.20594201511395482 1108 74-75 -0.8417673649204929 1108 76 0.7234835569471159 1109 1 -1.0368724821540667 1109 2 0.4540917514027356 1109 3 0.1135021359244277 1109 4 0.0762901681562198 1109 5 -0.05742576383597253 1109 6 -0.2610719701127451 1109 7 -0.13228921502279434 1109 8 0.5328297011551939 1109 9 0.6571198666600537 1109 10-11 0.2236340731372337 1109 12-13 0.1894109767255614 1109 14-15 0.7013322561444681 1109 16-17 0.6879870819315457 1109 18-19 0.7314294491535662 1109 20-21 1.0072565126423072 1109 22-23 0.8851548517603902 1109 24-25 -0.4345088064817233 1109 26-27 0.3793410457410644 1109 28-29 -0.9057172023778008 1109 30-31 0.054323146831414704 1109 32-33 1.1259407226246338 1109 34-35 -0.32957150999304474 1109 36-37 1.446477100260914 1109 38-39 0.9959474469972633 1109 40-41 0.6980897427516126 1109 42-43 1.0844185879851906 1109 44-45 0.8579652145529693 1109 46-47 0.8614380153855805 1109 48-49 0.9141154451718094 1109 50-51 0.15322788273857668 1109 52-53 -0.0644168573192303 1109 54-55 -0.4541193521845699 1109 56-57 0.29773209821469493 1109 58-59 -0.022415940239589816 1109 60-61 0.9178681607039678 1109 62-63 0.4428599158291249 1109 64-65 0.8295281001162991 1109 66-67 0.19443282936044426 1109 68-69 0.1684597212010459 1109 70-71 0.347061313059136 1109 72-73 0.3591415347571143 1109 74-75 -0.5952536281854464 1109 76 -0.2176482225788945 1110 1 -0.4427627379428998 1110 2 -0.40581710242091873 1110 3 -0.05021852475363886 1110 4 0.2698835929869645 1110 5 0.6069139532420849 1110 6 0.14131468988040297 1110 7 0.6989323017971003 1110 8 1.1846829189313368 1110 9 1.284632313645119 1110 10-11 -0.197719421238574 1110 12-13 0.9845955082184119 1110 14-15 1.2767254228155238 1110 16-17 1.1312973213878266 1110 18-19 0.4533018971923397 1110 20-21 1.2752157339483858 1110 22-23 -0.2311223801355098 1110 24-25 -0.6558280551843367 1110 26-27 1.4580270656035523 1110 28-29 1.1297797550258508 1110 30-31 0.6076015114688005 1110 32-33 1.4019081628922763 1110 34-35 1.2035414217000096 1110 36-37 1.6042179395433322 1110 38-39 1.2705187175117345 1110 40-41 -0.5676566812098258 1110 42-43 0.6266197944865937 1110 44-45 1.5798771768300455 1110 46-47 1.3063288243737503 1110 48-49 1.8199135010712837 1110 50-51 0.5482253801701518 1110 52-53 1.185879499467994 1110 54-55 0.5850392579679564 1110 56-57 0.9913650372837353 1110 58-59 0.0573237926441621 1110 60-61 0.6014381404199938 1110 62-63 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50-51 -1.2981267970643842 2116 52-53 -0.6415412415556858 2116 54-55 -0.7957141797707763 2116 56-57 -0.04124573429762535 2116 58-59 -2.3606240683184083 2116 60-61 -2.0668916915127795 2116 62-63 0.34233651681434907 2116 64-65 -2.180478057519167 2116 66-67 -1.9465770228563102 2116 68-69 -1.2219343674689043 2116 70-71 1.0262485051773638 2116 72-73 0.5190861160379114 2116 74-75 -0.21348022917066345 2116 76 -1.1138920649434283 2117 1 0.7681943721738733 2117 2 0.5336132151606563 2117 3 0.4185689902523677 2117 4 0.2511670154468604 2117 5 0.28142011934488664 2117 6 0.09853394121730474 2117 7 0.4257684908181645 2117 8 -0.11249049588914062 2117 9 -2.2190236942125665 2117 10-11 -1.3122561450189991 2117 12-13 -2.1221653779542393 2117 14-15 -0.4987029303439172 2117 16-17 -1.3025125662035748 2117 18-19 -1.8572405015853448 2117 20-21 -0.45614953943368874 2117 22-23 -0.2635080616808523 2117 24-25 -3.394396209718874 2117 26-27 -2.8400467093609834 2117 28-29 -2.0622970757064394 2117 30-31 -1.0077810051742162 2117 32-33 -2.7218777150952818 2117 34-35 -0.2280584909923391 2117 36-37 -2.3052822241585105 2117 38-39 -0.8648899914263879 2117 40-41 -0.23681525372973766 2117 42-43 -0.44707331912248804 2117 44-45 -1.1488961506827806 2117 46-47 -1.4689631105820524 2117 48-49 -1.9644911698880065 2117 50-51 -2.5863921031868244 2117 52-53 -0.20850552726997051 2117 54-55 -1.4022192818115968 2117 56-57 -0.7415008363384388 2117 58-59 -1.9428944764816762 2117 60-61 -1.2818151609005284 2117 62-63 -2.1838114423693256 2117 64-65 -1.4642790779273298 2117 66-67 -1.081781104488961 2117 68-69 0.12500440804129198 2117 70-71 -1.659975984618562 2117 72-73 0.4597748915481077 2117 74-75 0.6691728320538246 2117 76 0.596567118730043 2118 1 -0.554393753985682 2118 2 0.1917764804667783 2118 3 -0.47869446058251697 2118 4 0.057985197265040256 2118 5 0.0928765200870032 2118 6 -1.0037387860554219 2118 7 0.25508203441741983 2118 8 -1.000477508876152 2118 9 -2.6257954938415082 2118 10-11 -1.8972515068000746 2118 12-13 -2.689535507824374 2118 14-15 0.30881098431285636 2118 16-17 -4.564224068986505 2118 18-19 -1.0350002418450899 2118 20-21 -0.6017896136451952 2118 22-23 -1.7318227454410362E-4 2118 24-25 0.09250082182101238 2118 26-27 -3.6425745386745234 2118 28-29 -2.209792437487522 2118 30-31 -1.8552995580499143 2118 32-33 -0.7869287355034444 2118 34-35 -0.6199879900647005 2118 36-37 -4.556209867943295 2118 38-39 -2.099467913504313 2118 40-41 -2.5653171090173075 2118 42-43 -1.0281261948182205 2118 44-45 -4.065176299105779 2118 46-47 -0.858109678300039 2118 48-49 -0.6042175149715021 2118 50-51 -2.15932809576568 2118 52-53 -2.4585055272699705 2118 54-55 -2.4089446992513004 2118 56-57 -0.30910287715476414 2118 58-59 -1.4032377046820486 2118 60-61 -2.378174159045244 2118 62-63 -2.284692703964872 2118 64-65 -0.4917605250516388 2118 66-67 -0.8077783215576062 2118 68-69 -3.997096705131245 2118 70-71 -2.0865761701473176 2118 72-73 0.28410234980414373 2118 74-75 -1.4342594499498844 2118 76 -0.8695738831252484 2119 1 0.5620423461460717 2119 2 -1.6959046789535108 2119 3 -0.8362372800818534 2119 4 -0.9299924048429915 2119 5 -0.9840338883451452 2119 6 -1.2927704066087813 2119 7 -1.4142196783625565 2119 8 -0.050378694646902034 2119 9 -0.18146084298512477 2119 10-11 -0.443282468591903 2119 12-13 -0.5771343220536238 2119 14-15 -0.8374340749756826 2119 16-17 -0.8378571388152309 2119 18-19 -1.4038243525170273 2119 20-21 -2.258574857386961 2119 22-23 -1.5547680439346294 2119 24-25 0.5158374489619959 2119 26-27 -1.187946738938031 2119 28-29 -0.7223089065257255 2119 30-31 1.0271938543348043 2119 32-33 -0.2189939528947491 2119 34-35 0.1955587820038147 2119 36-37 -1.2630609878378962 2119 38-39 -1.958575291633423 2119 40-41 -2.319187332996229 2119 42-43 -0.5700398970580025 2119 44-45 -0.14142794600959974 2119 46-47 0.2845121925826959 2119 48-49 -1.2675080288054872 2119 50-51 0.5634156459997968 2119 52-53 -0.5599548026322907 2119 54-55 -0.903180535878441 2119 56-57 -1.9205159206330222 2119 58-59 -1.0150624741155099 2119 60-61 -0.17733423809662696 2119 62-63 -1.6004041664154656 2119 64-65 -1.0753408939580922 2119 66-67 0.08887846368613594 2119 68-69 -0.07441883951860007 2119 70-71 -0.006618989646653972 2119 72-73 0.7194613748370813 2119 74-75 -0.3822172892120719 2119 76 0.24933257273772824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 7.0 22 5.0 23 21.0 24 39.0 25 85.0 26 150.0 27 281.0 28 444.0 29 707.0 30 1231.0 31 1959.0 32 3012.0 33 4379.0 34 6706.0 35 9677.0 36 14753.0 37 15943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.5650578273093 24.40531304186798 12.169828790761098 27.859800340061614 2 17.358293631420345 19.804380397636404 38.908435884917765 23.928890086025486 3 17.491288025454114 26.130873217622597 29.763808690089395 26.614030066833894 4 12.804498240770357 19.396979848824095 39.60876079527281 28.189761115132743 5 12.444234945539637 37.8613154660696 36.69971885995185 12.994730728438917 6 27.630847965522463 39.90673557684214 18.046834228379993 14.4155822292554 7 26.585411020016497 30.701503341694586 23.74034107169913 18.97274456658979 8 22.316122624198247 36.88490092759381 21.07371929765492 19.72525715055302 9 23.050117001397282 15.418932341206377 23.390178616521606 38.14077204087473 10-11 24.54419959259945 26.16875136782209 28.104745711351658 21.182303328226798 12-13 24.210030134172825 23.654483931246947 26.870759751519337 25.264726183060894 14-15 21.56866046026161 25.700745778690592 24.3362906348378 28.394303126209998 16-17 22.0728607262504 28.060975404454467 26.507129509604216 23.359034359690913 18-19 21.737849531152676 27.948182690527094 27.024797562330598 23.28917021598963 20-21 21.103180081143417 27.98185215737109 26.92463089846972 23.990336863015774 22-23 22.536657632026397 27.624114072153667 27.22260568003906 22.61662261578088 24-25 22.11999797983199 28.960791905860173 26.62160569687379 22.29760441743405 26-27 21.502163263244725 28.74867426474302 27.273951616976145 22.47521085503611 28-29 22.373360717833034 27.21839699668356 28.3959865995522 22.012255685931216 30-31 21.75720947458797 28.433864749751685 27.517213514923995 22.29171226073635 32-33 21.35317587246006 28.350532819312807 27.590444605309678 22.70584670291746 34-35 22.17134391676908 27.545832561741385 28.138415178195654 22.144408343293883 36-37 21.722698271072876 27.39516169761452 27.996161680779785 22.885978350532817 38-39 21.451659062978738 28.02141378091278 27.9406070604872 22.586320095621286 40-41 20.94577532364775 27.050049662463593 29.096311509907242 22.907863503981414 42-43 22.190703860204376 26.789953031093756 28.590427770576255 22.42891533812562 44-45 20.35655965387788 27.010488038921903 28.797494991666806 23.83545731553341 46-47 21.332132455682565 26.00797966364203 29.252874530731805 23.407013349943604 48-49 20.95587616370095 26.31016312856686 29.674584602952812 23.059376104779382 50-51 20.30521371694079 26.957458628642616 28.8134879884177 23.923839665998887 52-53 20.348984023837982 26.318580495277853 30.23097254254979 23.10146293833437 54-55 19.470210939209778 26.954091681958214 29.607245669264827 23.96845170956718 56-57 20.347300550495785 26.785744347738255 28.892611235501086 23.974343866264878 58-59 19.27745324152792 27.582027238598677 29.065167253076545 24.075352266796855 60-61 19.77828656083231 28.33033113920641 28.043298934361378 23.848083365599905 62-63 18.81449807242302 28.576118247167553 27.985219104055485 24.624164576353934 64-65 19.410447635561688 29.311796097708793 26.720930624063566 24.55682564266595 66-67 19.218531674550935 28.91870507230518 26.43894883924513 25.423814413898754 68-69 19.064493863739667 29.108095823302637 26.470934832746924 25.356475480210772 70-71 19.41886500227269 28.348849345970606 26.25208329826097 25.980202353495734 72-73 20.09393781249474 28.361475396037108 25.2184306661504 26.326156125317755 74-75 20.49544620460935 28.217538425279038 25.10227100553863 26.184744364572985 76 20.925573643541355 28.036565040992578 25.223481086177003 25.814380229289068 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 1.0 18 1.0 19 2.5 20 7.5 21 11.0 22 24.0 23 40.0 24 50.0 25 57.0 26 129.5 27 212.0 28 273.5 29 325.0 30 428.0 31 599.5 32 833.5 33 999.0 34 1127.0 35 1463.5 36 1820.5 37 1969.0 38 2265.0 39 2868.0 40 3478.0 41 3962.5 42 4144.0 43 4305.5 44 4719.0 45 4824.0 46 4677.0 47 4696.0 48 4394.0 49 3765.5 50 3458.0 51 3185.5 52 2596.0 53 2069.0 54 1859.0 55 1697.5 56 1294.5 57 920.0 58 787.0 59 592.0 60 328.0 61 216.5 62 174.0 63 121.0 64 46.0 65 21.0 66 12.0 67 6.0 68 4.5 69 2.0 70 1.0 71 1.0 72 1.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59401.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11703506674972 #Duplication Level Percentage of deduplicated Percentage of total 1 67.12966202505073 32.30080301678423 2 13.22860541599608 12.730425413713572 3 6.675530053880065 9.636201410750662 4 3.960534602197187 7.6227672934799084 5 2.4281016024071094 5.841652497432703 6 1.7248618011335803 4.979714146226494 7 1.179063746413827 3.9713136142489183 8 0.7767126163319571 2.9898486557465365 9 0.5912812259463998 2.560562953485632 >10 2.295150794206144 15.918923923839667 >50 0.0 0.0 >100 0.01049611643691834 1.4477870742916785 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 406 0.6834901769330483 TruSeq Adapter, Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 333 0.5605966229524756 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 121 0.20370027440615476 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0016834733421996263 0.0 2 0.0 0.0 0.0 0.006733893368798505 0.0 3 0.0 0.0 0.0 0.006733893368798505 0.0 4 0.0 0.0 0.0 0.008417366710998131 0.0 5 0.0 0.0 0.0 0.008417366710998131 0.0 6 0.0 0.0 0.0 0.008417366710998131 0.0 7 0.0 0.0 0.0 0.010100840053197757 0.0 8 0.0 0.0 0.0 0.01346778673759701 0.0 9 0.0 0.0 0.0 0.016834733421996262 0.0 10 0.0 0.0 0.0 0.02188515344859514 0.0 11 0.0 0.0 0.0 0.025252100132994393 0.0 12 0.0 0.0 0.0 0.028619046817393648 0.0 13 0.0 0.0 0.0 0.033669466843992524 0.0 14 0.0 0.0 0.0 0.038719886870591406 0.0 15 0.0 0.0 0.0 0.04377030689719028 0.0 16 0.0 0.0 0.0 0.04545378023938991 0.0 17 0.0 0.0 0.0 0.04545378023938991 0.0 18 0.0 0.0 0.0 0.05387114695038804 0.0 19 0.0 0.0 0.0 0.05555462029258767 0.0 20 0.0 0.0 0.0 0.05555462029258767 0.0 21 0.0 0.0 0.0 0.057238093634787296 0.0 22 0.0 0.0 0.0 0.06228851366138617 0.0 23 0.0 0.0 0.0 0.06565546034578543 0.0 24 0.0 0.0 0.0 0.06902240703018468 0.0 25 0.0 0.0 0.0 0.07575630039898318 0.0 26 0.0 0.0 0.0 0.08417366710998131 0.0 27 0.0 0.0 0.0 0.09764145384757833 0.0 28 0.0 0.0 0.0 0.1026918738741772 0.0 29 0.0 0.0 0.0 0.11279271392737496 0.0 30 0.0 0.0 0.0 0.13804481406036936 0.0 31 0.0 0.0 0.0 0.15151260079796636 0.0 32 0.0 0.0 0.0 0.17508122758876113 0.0 33 0.0 0.0 0.0 0.1919159610107574 0.0 34 0.0 0.0 0.0 0.2239019545125503 0.0 35 0.0 0.0 0.0 0.27103920809413984 0.0 36 0.0 0.0 0.0 0.37036413528391776 0.0 37 0.0 0.0 0.0 0.5269271561084831 0.0 38 0.0 0.0 0.0 0.8585714045218094 0.0 39 0.0 0.0 0.0 1.252504166596522 0.0 40 0.0 0.0 0.0 1.6582212420666318 0.0 41 0.0 0.0 0.0 2.1952492382283126 0.0 42 0.0 0.0 0.0 2.710392080941398 0.0 43 0.0 0.0 0.0 3.33496069089746 0.0 44 0.0 0.0 0.0 3.892190367165536 0.0 45 0.0 0.0 0.0 4.425851416642818 0.0 46 0.0 0.0 0.0 5.062204339994276 0.0 47 0.0 0.0 0.0 5.744011043585125 0.0 48 0.0 0.0 0.0 6.356795340145789 0.0 49 0.0 0.0 0.0 6.898873756334068 0.0 50 0.0 0.0 0.0 7.533543206343327 0.0 51 0.0 0.0 0.0 8.164845709668187 0.0 52 0.0 0.0 0.0 8.87358798673423 0.0 53 0.0 0.0 0.0 9.656403090857056 0.0 54 0.0 0.0 0.0 10.329792427736907 0.0 55 0.0 0.0 0.0 10.90554031076918 0.0 56 0.0 0.0 0.0 11.513274187303244 0.0 57 0.0 0.0 0.0 12.046935236780525 0.0 58 0.0 0.0 0.0 12.531775559334019 0.0 59 0.0 0.0 0.0 13.078904395548896 0.0 60 0.0 0.0 0.0 13.686638272082961 0.0 61 0.0 0.0 0.0 14.195047221427249 0.0 62 0.0 0.0 0.0 14.836450564805306 0.0 63 0.0 0.0 0.0 15.489638221578762 0.0 64 0.0 0.0 0.0 16.077170418006432 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAACA 15 0.0022145032 70.00001 62 CAACGGA 15 0.0022145032 70.00001 59 CTATCGG 15 0.0022145032 70.00001 39 AACGGAA 15 0.0022145032 70.00001 60 ACGGAAC 15 0.0022145032 70.00001 61 TCCAATA 15 0.0022145032 70.00001 45 ACCAACG 15 0.0022145032 70.00001 57 TAAAGAA 20 7.866982E-5 70.0 2 CATGGTT 25 2.3725611E-4 55.999996 14 ACACGTA 25 2.3725611E-4 55.999996 38 ATGATTC 25 2.3725611E-4 55.999996 40 AATTTAC 25 2.3725611E-4 55.999996 53 TGAAGGT 25 2.3725611E-4 55.999996 28 TTGAGGC 25 2.3725611E-4 55.999996 11 ATTGGCG 25 2.3725611E-4 55.999996 21 GCGTGAA 25 2.3725611E-4 55.999996 25 TTGCACC 25 2.3725611E-4 55.999996 38 ATAAAGA 25 2.3725611E-4 55.999996 1 GCATGGT 25 2.3725611E-4 55.999996 13 CGCCATT 25 2.3725611E-4 55.999996 17 >>END_MODULE