FastQCFastQC Report
Wed 25 May 2016
SRR1780735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779926
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG602.0008883E-1036.64329528
CGGTTTA250.002361878535.170831
TATACGC250.002369321435.148253
ATGTCCG300.0057274529.3504234
GTCGTCC300.005743652629.333461
CTTCGGT300.005761698429.31463828
TCTTCGG300.005761698429.31463827
CTTACAT454.108745E-529.2902073
CATCGTT655.9697777E-727.05445932
TCCAGAT655.9965896E-727.0405832
CTGATAC508.421559E-526.3611873
TTCGGTA858.281859E-925.86585826
TTGCGAT701.1150041E-625.18500916
TCGTTGT701.1256743E-625.15750534
TCGTTCG901.506669E-824.4508323
TACCCGC901.506669E-824.4508311
TCAACGC450.001409800524.4085068
CTACCCG952.674824E-823.16394610
GCACGAC904.0027408E-722.04255521
TTTTCGA803.552741E-622.04255521
Produced by FastQC (version 0.11.5)