FastQCFastQC Report
Wed 25 May 2016
SRR1780735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779926
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT174162.23303236460895No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT66460.8521321253554824RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC33340.4274764528942489No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG14070.18040173042057836No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC13850.17758094998756294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC207.851542E-444.00384513
CCGTCTT7150.035.6931444
GACCGTG250.002351273835.2030811
GCGCATC250.002351273835.2030812
TCGACTT250.002351273835.2030815
GGTCGTC250.002352013535.2008242
CAACGCG250.002352013535.200829
ACGAGTT250.002352753735.19856334
ATCTGCG250.002352753735.19856316
TGCCGAC250.002352753735.19856321
ACAACGC250.002353493835.196318
GAAGACG250.002353493835.1963132
CGAGATT250.002353493835.1963129
CGCCATT2100.031.42527417
ATTGGCG2150.030.69642321
GCCGTCT8350.030.56358743
AACGCGA300.005741326629.33589710
CGCAAAG901.6370905E-1129.33589712
ACGCACA300.00574492629.33213424
CCTCGAC300.00574492629.33213427
Produced by FastQC (version 0.11.5)