FastQCFastQC Report
Wed 25 May 2016
SRR1780734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1971
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGTGGAGTGCTGCTAG50.2536783358701167No Hit
GCTTTATTTTTACATATGCAATAATAAAAAGTTTAACAATATAATTTCTG40.20294266869609334No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT30.15220700152207No Hit
ATTATGATGCGACTGTGAGTGCGCTCGTAGTTTGAGTTTGCTAGGCAGAA30.15220700152207No Hit
AACCAGGTTAAATCAAGTATCTTTTCTCATTTTTGCTTCCATCCATACAT30.15220700152207No Hit
AATGCAACTAAATCTGGGAGTGGTGGCAACCTGATTTATAGCCAGTGGCC30.15220700152207No Hit
TGCTAACACCCATTGGCCAAAACCAGTCACACGGCCAAGCACAGTCAATA30.15220700152207No Hit
TAAATGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGC30.15220700152207No Hit
GATGTGATTGGCTGAACCGCCGCTATTTGTTACCATGATACATTCCCAAG30.15220700152207No Hit
CCACAAAGGCAATACCTCTATGGGTCTGCAAGATCCAGAGTGTTACTGCT30.15220700152207No Hit
CTTCTTTATTGTCTGACTCCTCCATAAGCCATAAGGATAGAAATTTGATC30.15220700152207No Hit
GGGTATGGAGACATGTCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGT20.10147133434804667No Hit
GGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCATT20.10147133434804667No Hit
GATTTCAGCTGTCTTCAAATGGTTTCAGCCTATTTCAGGTGGTTTTCTGT20.10147133434804667No Hit
GTGTAGATTTCAGCCGCTTTGAGGTCAATGGTAGAAAAGGAAATATCTTC20.10147133434804667No Hit
GTCACTTTCTTGTTGTGTAAACATAGAAAAAGTATTTAATTTCTCAAAGG20.10147133434804667No Hit
CTCCGGATCCATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAA20.10147133434804667No Hit
AAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTCGAGTGCTATAGGC20.10147133434804667No Hit
AGCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCC20.10147133434804667No Hit
CTGTAAAGCTAACTTAGCATTAACCTTTTAAGTTAAAGATTAAGAGAACC20.10147133434804667No Hit
CTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTACT20.10147133434804667No Hit
GATCTGCTGCAGTGCTCTGAGCCCTAGGATTTATCTTTCTTTTCACCGTA20.10147133434804667No Hit
GTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGG20.10147133434804667No Hit
TAGTACATAAAGCCATCTACCGTACATAGCACATTACAGTCAAATCCCTT20.10147133434804667No Hit
AACAAAAAATACAAACCAAAACATTCAGCCTCTGTATTTAAGCTGTCTCT20.10147133434804667No Hit
CCGCACAAGAGTGCTACTCTCCTCGCTCCGGGCCCATAACACCTGTCTCT20.10147133434804667No Hit
GGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTC20.10147133434804667No Hit
CCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCAT20.10147133434804667No Hit
AGATAAAACAGACCCCCCTTTCCACCCGTTAGGCACATTGAACTTCTGCT20.10147133434804667No Hit
AACAAAGGCCTTTAAACGAAGAAATCACTACTTTTCGCCCTTGCCGTGGA20.10147133434804667No Hit
ATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAG20.10147133434804667No Hit
CCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAACGAAAAATTCT20.10147133434804667No Hit
GTCAATACGCCCGCCGGGAGTACCAGATCTGTCTCTTATACACTGTCTCT20.10147133434804667No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT20.10147133434804667No Hit
GTGTCTGCACTGTGCATGGGCCAGTGAGGTGTTTAAGGCAGGCAGATGGA20.10147133434804667No Hit
GGCCAAGGAGTAGGGGAATAGACTTCACCTCCTGCTGGGAGGAACTGCAA20.10147133434804667No Hit
GGTTTGGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCTAGG20.10147133434804667No Hit
CCCATTCCCTCTTGGACCCATTCTGTTCAGGCTTGTGTTCTTGCTGTGCC20.10147133434804667No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG20.10147133434804667No Hit
CATCTGACGCTGCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCAG20.10147133434804667No Hit
TCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCAC20.10147133434804667No Hit
CTTCAACCCCTCTGGGATTATACCATGTGCTACACCTAAGGTCTGTCTCT20.10147133434804667No Hit
CTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTA20.10147133434804667No Hit
GGGATAAGGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATT20.10147133434804667No Hit
TCCTAGAAGTGAGATGGTAAATGCTAGTATAATATTTATGTAAATGAGGG20.10147133434804667No Hit
CTCCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGC20.10147133434804667No Hit
AGCCTGTAATGGGCAGACTTAGGAAAAGGAACAAAAAAGGAGGCAGAGAA20.10147133434804667No Hit
TTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAGGAAGGAATCGAA20.10147133434804667No Hit
GTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAATGA20.10147133434804667No Hit
CTCCTCACTCTCTCAATCTTATCCATCATAGCAGGCAGTTGAGGTGGATT20.10147133434804667No Hit
GCCCTAACATCTCCCCATATGACTGTATTGCAGACAGGGCCTTTAAAGAA20.10147133434804667No Hit
AAACAACCCCCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACC20.10147133434804667No Hit
CCTCTACACTTATCATCTTCACAATTCTAATCCTACTGACTATCCTAGAA20.10147133434804667No Hit
TAGCCAAGGTGGGGATAAGTGTGGTTTCGAAGAAGATATAAAATATGATT20.10147133434804667No Hit
CAACCTCCCTCACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTC20.10147133434804667No Hit
GTTTCCATCCACCCTTGGCCAAATTACCAACCTCCCTATGCATCAGTTTC20.10147133434804667No Hit
TTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCA20.10147133434804667No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC20.10147133434804667No Hit
TGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGA20.10147133434804667No Hit
CTCCTGCTCGCATCTACTATAGTGGAGGCCGGAGCAGGAACAGGTTGAAC20.10147133434804667No Hit
GTTAGTGTGTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGCTGTCTC20.10147133434804667No Hit
AAATCGCTCATTGCATACTCTTCAATCAGCCACATAGCCCTCGTAGTAAC20.10147133434804667No Hit
CTATAATGTATATGAATCTTGAAATATTATGCTAAGTAAAAAGCCAAACA20.10147133434804667No Hit
TTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGT20.10147133434804667No Hit
CTTCTCCACCACTATCAGACCCTTAGGCCCCACCTCTTCACTTTGGAACG20.10147133434804667No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA20.10147133434804667No Hit
GACCACATCTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTT20.10147133434804667No Hit
CCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCAT20.10147133434804667No Hit
GATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGCTGTCTCT20.10147133434804667No Hit
ATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAA20.10147133434804667No Hit
ACTTCCCACTGGGTCCCTCCTGTGACACATGGGGATTATGGGAGCTACAA20.10147133434804667No Hit
CTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTA20.10147133434804667No Hit
AATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATTT20.10147133434804667No Hit
CTACACGATGATGGGAGGATGGACGCCGCCTTCAGGAGCTGGAGGAGCAG20.10147133434804667No Hit
AGCTTACACAATAGCTTTTATAGTAAAGATACCTCTTTACGGACTCCACT20.10147133434804667No Hit
GTGTTAAATTTTTTACTCTCTCTGCAAGGTTTTTTCCTAGTGTCCAAAGA20.10147133434804667No Hit
ATATATCACAGTGAGAATTCTATGATGGACCATGTAACGAACAATGCTAC20.10147133434804667No Hit
GTGTGTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGA20.10147133434804667No Hit
CTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAG20.10147133434804667No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG20.10147133434804667No Hit
ACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCAT20.10147133434804667No Hit
GTACGAAAGGACAAGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCCC20.10147133434804667No Hit
CTATCCTTCCTTGCATCTGCTTGGACTATTGCAATAGCCTCTAAGCTGTT20.10147133434804667No Hit
GTATATAGTTTAAACAAAACGAATGATTTCGACTCATTAAATTATGATAA20.10147133434804667No Hit
TAGCATGATTTATCCTACACTCCAACTCATGAGACCCACAACAAATAGCC20.10147133434804667No Hit
GTGCCTTGGGTAACCTCTGGGACTCAGAAGTGAAAGGGGGCTATTCCTAG20.10147133434804667No Hit
CTCCAACATACTCGGATTCTACCCTAGCATCACACACCGCACAATCCCCT20.10147133434804667No Hit
GCTCAGAGTGACTTTTGGCTATTTCAAAATATGCACCTCAAAAGATCATA20.10147133434804667No Hit
ACTTACTACTCCGGAAAAAAAGAACTGTCTCTTATACACATCTGACGCTG20.10147133434804667No Hit
CCCTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCA20.10147133434804667No Hit
CATCGCACTGATTTACACTCACAACACCCTAGGCTCACTAAACATTCTAC20.10147133434804667No Hit
GGAGGAGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTG20.10147133434804667No Hit
GAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCTGTCTCT20.10147133434804667No Hit
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT20.10147133434804667No Hit
CCATTGGGTGGAGGCTGTAAACAGGAAACTTGAAACCCTCAGTCCTGTCT20.10147133434804667No Hit
ATGTACGGTAGATGGCTTTATGTACTATGTACTGTTAAAGATGGGTAGGT20.10147133434804667No Hit
CTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAGGAATCACCTC20.10147133434804667No Hit
GTTAACCTGAGGTCTTTCTAACTTTTTGATGTGGGCCTTTAGTGCTATGA20.10147133434804667No Hit
CTACAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCCTGT20.10147133434804667No Hit
CTCCATAACGCTCCTCATACTAGGCCTACTAACCAACACACCTGTCTCTT20.10147133434804667No Hit
ATAATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGTT20.10147133434804667No Hit
GGATTATAGGCTCGAGTCACCACGCCTGGCTCTGAGCCCTTTTCTTAAAA20.10147133434804667No Hit
ATGCCAGGCAAGGGTTAAGTCAGTCACCCCACACTTAAAGAATGAACCTG20.10147133434804667No Hit
ATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATG20.10147133434804667No Hit
CCCTTCGCTGACGCCATAAAACTCTTCACCAAAGAGCCCCTAAAACCCGC20.10147133434804667No Hit
GACAGGAATGGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGA20.10147133434804667No Hit
CCTTCACGCCAATGGCGCCTCAATATTCTTTATCTGCCTCTTCCTACACA20.10147133434804667No Hit
TGCTTACAAGCAAGTACAGCAATCAACCCTCAACTATCACACATCAACTG20.10147133434804667No Hit
CCACCACACATACACACACATGCAATAATGCAACTTCACTGAAGAAAAAC20.10147133434804667No Hit
ATATTGTGCCTATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTG20.10147133434804667No Hit
GCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCTCTGAC20.10147133434804667No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)