FastQCFastQC Report
Wed 25 May 2016
SRR1780733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62766
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT980.1561354873657713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTTC207.7446556E-444.063119
CCCCACA207.7446556E-444.063114
CTAAGGT207.775215E-444.0279338
TAAGGTC207.775215E-444.0279339
TTTACAC301.294418E-436.6314772
CATCTTC250.00231948135.2504814
TTTCTCG250.002328598435.22234740
TGAAGTC250.002346918235.1662183
GAGACAT250.002346918235.1662183
AAGAGCG300.00566422129.37539921
AGTAGAA300.00566422129.37539916
GAGCTAA300.005686400529.35195535
GTTCATA300.005686400529.35195510
AGAGCTA300.005686400529.35195534
ACTATGC300.005730965629.3051828
GTTAGTT300.005730965629.30518243
GGGGTTC300.005730965629.3051827
GAAGTCC300.005730965629.3051824
GGGTTCA300.005730965629.3051828
ATTATAT300.005730965629.30518233
Produced by FastQC (version 0.11.5)