##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780733_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 62766 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.40216040531498 31.0 16.0 34.0 10.0 34.0 2 26.59957620367715 31.0 22.0 34.0 10.0 34.0 3 26.745068986393907 31.0 22.0 34.0 10.0 34.0 4 29.508603383997706 35.0 24.0 37.0 10.0 37.0 5 29.418395309562502 35.0 23.0 37.0 10.0 37.0 6 29.559777586591466 35.0 25.0 37.0 10.0 37.0 7 29.507249147627697 35.0 25.0 37.0 10.0 37.0 8 29.451852913998025 35.0 24.0 37.0 8.0 37.0 9 30.754819488257976 38.0 25.0 39.0 10.0 39.0 10 30.53713794092343 38.0 23.0 39.0 8.0 39.0 11 30.701319185546314 38.0 25.0 39.0 8.0 39.0 12 30.556925724118152 38.0 23.0 39.0 8.0 39.0 13 30.61173246662206 38.0 24.0 39.0 8.0 39.0 14 31.603065353853996 39.0 25.0 41.0 8.0 41.0 15 31.46346748239493 39.0 23.0 41.0 8.0 41.0 16 31.351209253417455 39.0 23.0 41.0 8.0 41.0 17 31.394305834368925 39.0 23.0 41.0 8.0 41.0 18 31.197463594939936 38.0 22.0 41.0 8.0 41.0 19 31.18212089347736 39.0 22.0 41.0 8.0 41.0 20 31.181483605773828 39.0 22.0 41.0 8.0 41.0 21 31.053532167096837 38.0 21.0 41.0 8.0 41.0 22 31.072459611891787 38.0 22.0 41.0 8.0 41.0 23 31.006038300990983 38.0 21.0 41.0 8.0 41.0 24 30.78013574228085 38.0 20.0 41.0 8.0 41.0 25 30.7232100181627 38.0 20.0 41.0 8.0 41.0 26 30.537376923812253 38.0 19.0 41.0 8.0 41.0 27 30.391119395851256 38.0 18.0 41.0 8.0 41.0 28 30.340757735079503 38.0 18.0 41.0 8.0 41.0 29 30.251744575088424 38.0 18.0 41.0 8.0 41.0 30 30.12392059395214 38.0 18.0 41.0 8.0 41.0 31 30.089682312079788 38.0 18.0 41.0 8.0 41.0 32 29.950403084472484 38.0 17.0 41.0 8.0 41.0 33 29.900583118248733 38.0 17.0 41.0 8.0 41.0 34 29.706895452952235 37.0 16.0 41.0 7.0 41.0 35 29.621753815760126 37.0 16.0 41.0 7.0 41.0 36 29.606761622534492 37.0 16.0 41.0 7.0 41.0 37 29.537838957397316 37.0 15.0 41.0 7.0 41.0 38 29.617213140872447 37.0 15.0 41.0 7.0 41.0 39 29.476006117961955 37.0 15.0 41.0 7.0 41.0 40 29.21530765063888 37.0 15.0 41.0 7.0 41.0 41 29.262881177707676 37.0 15.0 41.0 7.0 41.0 42 29.29812318771309 37.0 15.0 41.0 7.0 41.0 43 29.281187266991683 37.0 15.0 41.0 7.0 41.0 44 29.225679508013894 37.0 15.0 41.0 7.0 41.0 45 29.264123888729568 36.0 15.0 41.0 7.0 41.0 46 29.1257527960998 36.0 15.0 41.0 7.0 41.0 47 29.07553452506134 36.0 15.0 41.0 7.0 41.0 48 28.97978204760539 36.0 14.0 41.0 7.0 41.0 49 28.854379759742535 35.0 14.0 41.0 7.0 41.0 50 28.82441130548386 35.0 14.0 41.0 7.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5862113990035205 1101 2 0.5191334588984446 1101 3 -0.31156886390469296 1101 4 0.6457900485129962 1101 5 -0.22429577202775874 1101 6 -0.9413360872683825 1101 7 -1.6093321151916555 1101 8 -1.8650141922173198 1101 9 -0.29937610527459313 1101 10 0.4608052305749375 1101 11 -0.3341865368757375 1101 12 -0.02307202269552633 1101 13 -0.14244633055981026 1101 14 -0.6113194304052385 1101 15 -0.6388827517369506 1101 16 -0.25746024195199624 1101 17 -0.7948975350777481 1101 18 -0.5624457928408617 1101 19 0.2667397141754293 1101 20 -0.4051884342494745 1101 21 0.44826615047413654 1101 22 -0.42488696992452546 1101 23 -0.8829008231141842 1101 24 0.06120720920536016 1101 25 -0.6642924978386944 1101 26 -0.60364071857936 1101 27 -0.7406759369961655 1101 28 -0.49280870093580376 1101 29 -0.23753350382212446 1101 30 -0.12536474988568713 1101 31 -0.3395965513384134 1101 32 -0.07229984611185358 1101 33 0.42799681284448 1101 34 0.891039575598473 1101 35 0.7832467118416879 1101 36 -0.34857838372563776 1101 37 -0.09617137568026024 1101 38 -1.1732617415076518 1101 39 0.12808819818951278 1101 40 -0.04374404628190831 1101 41 -0.4855570542687886 1101 42 0.18952349069115115 1101 43 -0.28970837565323393 1101 44 0.6111094860497417 1101 45 -0.09272341081506141 1101 46 -0.1787833012727269 1101 47 0.17596572965478074 1101 48 0.2371862522426298 1101 49 0.28250360872549507 1101 50 0.17671024814930902 1102 1 -0.5620750457662744 1102 2 -0.2678718030553391 1102 3 -0.6558836660094762 1102 4 0.06079805583397757 1102 5 -1.5116442048806533 1102 6 -0.9145687571379781 1102 7 -0.2767995139562416 1102 8 -0.8169702663422349 1102 9 -1.0677357483768581 1102 10 -1.0267262267574822 1102 11 -0.6762135329864698 1102 12 -1.5275332443839247 1102 13 -1.7743383461168918 1102 14 0.45491255312255774 1102 15 -0.4861717016339995 1102 16 -0.8522898004054404 1102 17 -0.08693597021844823 1102 18 -0.5888699520721588 1102 19 -0.6194419376204969 1102 20 -0.8581740210717115 1102 21 -0.2781122526372819 1102 22 -0.22870760593459138 1102 23 -0.5216562566534186 1102 24 -0.6163265359330516 1102 25 -0.7909225596094558 1102 26 -0.49531310476101 1102 27 -0.6396693023587829 1102 28 -1.0534355597781726 1102 29 -0.9809560615892252 1102 30 -0.4638456467056358 1102 31 -0.5063776083047813 1102 32 0.09036316006521972 1102 33 -0.41300067056892686 1102 34 -1.7307860935847827 1102 35 -2.0175997763761124 1102 36 -1.476695519392532 1102 37 -1.3744829748566474 1102 38 -1.772666911949198 1102 39 -1.1848836617967606 1102 40 -2.097278706542717 1102 41 -1.6616037255110676 1102 42 -1.984349764856777 1102 43 -1.905700139551655 1102 44 -1.6732647990108838 1102 45 -2.0497126581268894 1102 46 -1.8891470624257813 1102 47 -1.4953909392996927 1102 48 -1.8911596640236716 1102 49 -2.1433714770672303 1102 50 -1.6845343183114565 1103 1 -0.016423320916036488 1103 2 -0.9253805822331955 1103 3 -1.1237622035017303 1103 4 -1.7803504671984562 1103 5 -0.7787377805881519 1103 6 -2.114527443359833 1103 7 -0.4580637155674765 1103 8 -0.5916036065611969 1103 9 -1.4669301297030302 1103 10 -2.6001614674102385 1103 11 -1.6983164042940508 1103 12 -1.2773783177158826 1103 13 -1.0063152104011301 1103 14 -1.356386765200103 1103 15 -1.254917828467299 1103 16 -1.4724096103620603 1103 17 -0.9843745159734603 1103 18 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0.39338484194819756 1104 5 0.7333725924317775 1104 6 0.5011087344633651 1104 7 0.5224166114636937 1104 8 1.4195358926723216 1104 9 0.9063963904809249 1104 10 0.7170587676434153 1104 11 1.1994863550314498 1104 12 1.0207310333316393 1104 13 1.7228621018114403 1104 14 0.4671186001550325 1104 15 1.0317454316246817 1104 16 0.9338244213190876 1104 17 0.2377000880122324 1104 18 0.7747358709961496 1104 19 1.8604012874634854 1104 20 1.2246927202496742 1104 21 0.558169942211542 1104 22 0.5843942977608165 1104 23 0.6109305294608021 1104 24 1.0151404293525026 1104 25 1.2745869588675873 1104 26 1.0633655066611603 1104 27 1.9223687715493938 1104 28 0.42931919946034824 1104 29 0.8807460660703477 1104 30 0.878483580617992 1104 31 0.9408788306649853 1104 32 0.6074964187438319 1104 33 0.9742333719842655 1104 34 -0.48688911710101834 1104 35 1.251045240421199 1104 36 1.0926213899019785 1104 37 0.5635909333180429 1104 38 0.3984417106711824 1104 39 0.27715441211141467 1104 40 0.6397873628861745 1104 41 0.018687427904389153 1104 42 0.6559637849752491 1104 43 0.4070658178951554 1104 44 0.3957161640350293 1104 45 -0.3760284476005751 1104 46 0.8049178871822811 1104 47 0.542123058460664 1104 48 0.7095122284735282 1104 49 1.008140281051471 1104 50 0.34603679031116386 1105 1 -0.8943172716255212 1105 2 -1.1420534724497742 1105 3 -1.8983141079080035 1105 4 -1.11268803254573 1105 5 -1.033125383276726 1105 6 -1.1559762857795484 1105 7 -1.1397291375241636 1105 8 -0.9354236214977156 1105 9 -1.4097979415028803 1105 10 -1.5786902202108344 1105 11 -1.1289549405151114 1105 12 -0.7212730152513558 1105 13 -1.2422188698484788 1105 14 -0.25089759417695134 1105 15 -1.6782748112903008 1105 16 -0.9983618134987289 1105 17 -0.4292027459958625 1105 18 -0.7290090682751043 1105 19 -2.5920278623340813 1105 20 -1.562756672462875 1105 21 -1.7159191266143665 1105 22 -1.6667190625630681 1105 23 -1.1759115758023562 1105 24 -1.2808109876515452 1105 25 -1.8944413812133831 1105 26 -1.132383481193088 1105 27 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1.2105614012205983 1107 37 -0.6875692251426244 1107 38 1.020286645589124 1107 39 0.24636776808198846 1107 40 0.6659333730729315 1107 41 0.32089455863443916 1107 42 0.028233168110507023 1107 43 0.323194850153218 1107 44 0.17024927099597775 1107 45 0.46641637413117465 1107 46 0.03627046216813312 1107 47 1.2619872350311248 1107 48 0.6780464672963902 1107 49 1.0459444689405508 1107 50 1.0261726137407052 1108 1 -1.7663737865391802 1108 2 -1.4389530014921625 1108 3 -2.003690873999055 1108 4 -1.4250899163761837 1108 5 -0.9595382561181687 1108 6 -1.6803090958266438 1108 7 -1.4022880072258879 1108 8 -1.1243953640408897 1108 9 -1.8313050058670903 1108 10 -2.0508252280601305 1108 11 -1.6683976406720973 1108 12 -1.6465084940582635 1108 13 -1.3464182419049955 1108 14 -1.9977578767515176 1108 15 -2.2534317971615714 1108 16 -1.4775611855552455 1108 17 -2.042428805650495 1108 18 -1.9136636993583096 1108 19 -1.452702901580551 1108 20 -1.434813187376534 1108 21 -2.2904874154683696 1108 22 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1.397862924667681 2213 22 1.4847232451292385 2213 23 1.2199217575309795 2213 24 1.6647018328612724 2213 25 1.0783150290430257 2213 26 1.7562383136787076 2213 27 2.9206143855844786 2213 28 1.4853499012147324 2213 29 1.7765793994036798 2213 30 1.9636809490390448 2213 31 1.5663174271562106 2213 32 1.8534394011999744 2213 33 1.0200667053175962 2213 34 1.824843339039333 2213 35 1.889093486035236 2213 36 1.2334108635861902 2213 37 0.7842049684057635 2213 38 1.6597355703203043 2213 39 1.9795666928131688 2213 40 1.9344145558686314 2213 41 2.1431391822903514 2213 42 2.907937469185775 2213 43 1.6403991512284861 2213 44 2.2445100236841498 2213 45 1.8565373418731106 2213 46 2.088689817006845 2213 47 1.849688847934349 2213 48 2.037253456543702 2213 49 1.7294122108760348 2213 50 1.9280543341708132 2214 1 -0.21838429371234724 2214 2 -0.5880740360445955 2214 3 0.42184963923594054 2214 4 -0.10583350093872212 2214 5 -0.9875237077245558 2214 6 -0.009365470851903979 2214 7 -0.2720596782705442 2214 8 0.7303227608328235 2214 9 -0.6835983984716556 2214 10 0.2863135878622778 2214 11 -0.6629419930665179 2214 12 -0.3632189406131232 2214 13 -0.8175235156976797 2214 14 0.06003157566311046 2214 15 -0.15533117077240277 2214 16 -0.38926051875386847 2214 17 0.0010143141716909554 2214 18 0.19130700179344018 2214 19 0.5795258314968379 2214 20 0.053770364856241315 2214 21 0.5746368520604221 2214 22 0.6793395817629033 2214 23 -0.7476937540201796 2214 24 -0.4487470120232153 2214 25 -0.19128068052793168 2214 26 0.9256896338107197 2214 27 0.19949227337325937 2214 28 0.46173191441605255 2214 29 0.36589458092183236 2214 30 0.3841636223063709 2214 31 0.151300925506046 2214 32 0.20812174443429754 2214 33 -0.19012306365929987 2214 34 0.3404167713825679 2214 35 -0.04087763607697781 2214 36 0.2519752200218335 2214 37 -0.10894684677575484 2214 38 0.11631560332360635 2214 39 0.9067531614600348 2214 40 0.42894838425146986 2214 41 0.5007093143035526 2214 42 0.3305859840372598 2214 43 0.252610372350599 2214 44 0.2561643141131924 2214 45 0.10870318345726915 2214 46 0.3638548335084941 2214 47 -0.16918491444188888 2214 48 0.04395725192324207 2214 49 0.6660871283677814 2214 50 0.25932496123352067 2215 1 1.4026058052975543 2215 2 0.6106388352425327 2215 3 0.9328397382458427 2215 4 1.1065427366850393 2215 5 1.434951539800199 2215 6 0.9047929449896799 2215 7 0.3287324009373762 2215 8 1.3011148400407428 2215 9 1.004817443112504 2215 10 0.992848241327625 2215 11 1.825275828715661 2215 12 0.7875731385947979 2215 13 0.2597042070745985 2215 14 1.0344870212076671 2215 15 0.8559559579404699 2215 16 1.2785612634243506 2215 17 0.8334895616964424 2215 18 0.6036832394172009 2215 19 0.10566444535822583 2215 20 1.0041664044602001 2215 21 1.6886962580010199 2215 22 1.415973245129237 2215 23 1.614088424197643 2215 24 1.823035166194611 2215 25 1.4624816957096947 2215 26 1.9691549803453725 2215 27 1.313947718917813 2215 28 0.7082665678813989 2215 29 0.7207460660703475 2215 30 1.347430949039044 2215 31 1.002984093822878 2215 32 1.3080227345333064 2215 33 1.844233371984263 2215 34 1.5194266723726635 2215 35 0.7536768193685681 2215 36 1.2284108635861912 2215 37 0.5825383017390955 2215 38 0.3026522369869724 2215 39 -0.21126664052016508 2215 40 -0.4060021107980347 2215 41 0.3760558489570194 2215 42 -0.314562530814225 2215 43 -0.4129341821048449 2215 44 0.059926690350817324 2215 45 0.23028734187311173 2215 46 0.6870231503401776 2215 47 0.7184388479343475 2215 48 0.6426701232103689 2215 49 0.620245544209368 2215 50 0.1097210008374816 2216 1 -0.5514367478939342 2216 2 -0.0338292498638495 2216 3 -0.3899262192009658 2216 4 -0.7477125824638975 2216 5 -0.4371761197742714 2216 6 -0.8507389699039365 2216 7 0.13809410306503622 2216 8 -0.242502181235853 2216 9 -0.1847570249726047 2216 10 -0.6331092054808849 2216 11 -0.2187667244758309 2216 12 -0.8360438826817997 2216 13 -1.2211468567551904 2216 14 -0.7791300000689318 2216 15 -0.15638446759144387 2216 16 -1.0756940557245898 2216 17 -0.2784253319205767 2216 18 -0.6739763350508845 2216 19 -1.215186618471563 2216 20 -0.6134931700078852 2216 21 -1.0227931037011118 2216 22 -0.7818990952962928 2216 23 -0.340805192823634 2216 24 -0.669518025294753 2216 25 -0.7164544745030739 2216 26 -0.5804194877397357 2216 27 -0.3311586640609079 2216 28 -0.18109513424625945 2216 29 -1.1511688275466732 2216 30 -0.974483944577976 2216 31 -0.8255265444749931 2216 32 -2.1649559888709504 2216 33 -1.3066176918455241 2216 34 -0.8690839659252063 2216 35 -0.06015296786547353 2216 36 -1.1043550938606188 2216 37 -1.917036166346012 2216 38 -1.2620286140768577 2216 39 -0.5359474915839932 2216 40 -0.6185553022873975 2216 41 -1.2360718106174495 2216 42 -0.5056263606014575 2216 43 -0.7248490757218669 2216 44 -0.6413499053938629 2216 45 -0.16673393472263243 2216 46 -0.7614874879576945 2216 47 -0.42092285419331077 2216 48 -0.6996703023215431 2216 49 -0.7603927536629733 2216 50 -0.493044956609328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 27.0 7 512.0 8 1516.0 9 1945.0 10 1676.0 11 1413.0 12 1369.0 13 1409.0 14 1339.0 15 1349.0 16 1239.0 17 1157.0 18 1063.0 19 989.0 20 930.0 21 871.0 22 836.0 23 781.0 24 764.0 25 716.0 26 619.0 27 700.0 28 681.0 29 661.0 30 644.0 31 658.0 32 707.0 33 816.0 34 1028.0 35 1408.0 36 1947.0 37 2942.0 38 5512.0 39 22541.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.424390321705985 20.94358581750323 14.987957956521045 27.644065904269745 2 18.479334629234252 24.511647175042224 34.380676205347186 22.62834199037634 3 23.40635704612443 23.095674340795032 29.138851270612605 24.359117342467936 4 17.75802185896823 19.46595290443871 31.386419399037695 31.38960583755536 5 17.049039288786926 31.945639358888574 30.73160628365676 20.273715068667748 6 32.2945543765733 31.959978332218082 15.968836631297197 19.77663065991142 7 32.444316986903736 27.650320237071025 18.4606315521142 21.444731223911035 8 27.215371379409238 29.992352547557594 21.017748462543416 21.774527610489756 9 27.82442444037282 15.170875487931172 20.60861945351709 36.39608061817892 10 24.267915750565592 24.347576713507312 28.39594685020553 22.988560685721566 11 34.24042061658567 20.73926551421971 21.658567673066198 23.361746196128415 12 23.834939855014735 22.751533497968612 27.032581852943522 26.38094479407313 13 29.128162641004394 18.22063603339494 24.251163087120005 28.400038238480658 14 28.443056529220573 19.02045758715187 21.49002612962845 31.046459753999105 15 25.65005417118093 25.849212924606462 22.092282199987253 26.408450704225352 16 28.90055363211385 23.41209694146178 22.140498109354308 25.546851317070058 17 32.00560831049646 22.70728443056529 21.905869606780957 23.38123765215729 18 28.876834716017868 22.36917677089981 22.38353541799617 26.370453095086148 19 27.82272865099504 22.39989786310464 24.032492299835624 25.7448811860647 20 29.377295225930066 21.68928628452818 23.74740539677471 25.186013092767045 21 25.884945154955215 23.735849659114496 21.778352573006977 28.600852612923312 22 28.451189185742525 22.27890072052541 21.677931518204424 27.59197857552764 23 31.770235818992987 22.53983428935628 22.262587635436585 23.42734225621415 24 27.01223695336556 25.81406850779368 21.997798305652612 25.17589623318815 25 31.042891981064408 22.437399384752705 21.404549004606384 25.1151596295765 26 28.828254494453653 26.07739385439245 21.37415529771771 23.720196353436187 27 26.69418589825322 25.68532449317863 24.003888818054318 23.616600790513832 28 29.373047746541726 24.625486071269204 23.288391661885637 22.713074520303437 29 27.691179752000256 24.293136973638074 24.479614930987218 23.536068343374453 30 29.224858108539 23.0198966902621 24.045022638862317 23.710222562336586 31 28.70736372330252 22.811603442779727 24.303474657315906 24.17755817660185 32 27.133772174495146 24.319025836374937 23.946063977303517 24.6011380118264 33 29.1026458399745 23.27861013707364 24.743385400063755 22.875358622888108 34 27.90334406579744 23.87548216391979 24.839810003506646 23.38136376677612 35 28.292263062258915 24.889221843221016 23.328764066435014 23.489751028085053 36 30.083840734483086 23.231534317319646 24.16079569001243 22.523829258184833 37 28.14975375739126 23.357187255948872 25.27293881389159 23.220120172768276 38 28.046350754713973 23.626452445847082 25.9886195189595 22.338577280479445 39 27.879879496947574 23.547507850231923 26.410252323190463 22.162360329630044 40 25.764805387549274 26.69358313518344 25.737676140625247 21.803935336642038 41 27.369092213278073 20.98509603889376 26.489200605722484 25.156611142105685 42 24.505053081263746 22.593808779927947 29.001817196416617 23.899320942391686 43 25.330719454273055 21.57247139077492 26.9404864365178 26.15632271843422 44 23.818706274203574 20.5804076558989 31.16862420118249 24.432261868715038 45 24.95497936222091 21.017068001083683 31.1144400707581 22.913512565937307 46 25.358127380212565 21.588030020555475 27.624010070589733 25.429832528642223 47 26.354538500764917 22.155469148393678 27.83656297807241 23.653429372768993 48 25.859294080152978 24.085730220699546 26.743685762090667 23.31128993705681 49 25.207553183013303 22.49063819615967 27.843199745040238 24.458608875786787 50 26.124647427214637 23.603652415023983 26.382802415820755 23.888897741940625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 4.5 2 3.0 3 3.5 4 4.0 5 3.5 6 3.0 7 5.0 8 7.0 9 6.0 10 5.0 11 5.0 12 5.0 13 5.5 14 6.0 15 5.5 16 5.0 17 23.5 18 42.0 19 43.5 20 45.0 21 126.5 22 208.0 23 191.0 24 174.0 25 258.0 26 342.0 27 413.5 28 485.0 29 821.5 30 1158.0 31 1194.0 32 1230.0 33 1325.0 34 1420.0 35 1826.5 36 2233.0 37 2351.0 38 2469.0 39 2710.5 40 2952.0 41 3198.0 42 3444.0 43 3389.0 44 3334.0 45 3670.5 46 4007.0 47 3967.0 48 3927.0 49 3927.0 50 3927.0 51 3897.0 52 3867.0 53 3841.5 54 3816.0 55 3631.5 56 3447.0 57 3216.5 58 2986.0 59 2890.0 60 2794.0 61 2574.5 62 2355.0 63 2125.0 64 1895.0 65 1806.0 66 1717.0 67 1651.5 68 1586.0 69 1407.5 70 1229.0 71 1225.5 72 1222.0 73 1129.0 74 1036.0 75 888.0 76 740.0 77 755.5 78 771.0 79 679.5 80 588.0 81 502.5 82 417.0 83 359.0 84 301.0 85 256.0 86 211.0 87 180.5 88 150.0 89 118.5 90 87.0 91 67.5 92 48.0 93 41.0 94 34.0 95 24.5 96 15.0 97 12.5 98 10.0 99 6.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10993212885957367 2 0.006372877035337603 3 0.0015932192588344008 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0015932192588344008 10 0.0 11 0.0015932192588344008 12 0.0015932192588344008 13 0.0031864385176688017 14 0.0031864385176688017 15 0.0031864385176688017 16 0.14179651403626167 17 0.0031864385176688017 18 0.13701685625975846 19 0.16728802217761207 20 0.2166778192014785 21 0.21508459994264412 22 0.054169454800369624 23 0.009559315553006405 24 0.13861007551859286 25 0.04301691998852882 26 0.03505082369435682 27 0.03505082369435682 28 0.028677946659019213 29 0.03823726221202562 30 0.06691520887104482 31 0.041423700729694424 32 0.03983048147086002 33 0.041423700729694424 34 0.04461013924736322 35 0.04461013924736322 36 0.04461013924736322 37 0.03664404295319122 38 0.04301691998852882 39 0.046203358506197625 40 0.16410158365994326 41 0.04938979702386642 42 0.05098301628270083 43 0.03823726221202562 44 0.027084727400184812 45 0.027084727400184812 46 0.014338973329509607 47 0.02230506962368161 48 0.01752541184717841 49 0.01752541184717841 50 0.020711850364847212 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 62766.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.58576299270306 #Duplication Level Percentage of deduplicated Percentage of total 1 88.07312919705684 43.67173310390976 2 1.6482986858593323 1.6346429595640952 3 1.0378176910966166 1.5438294618105344 4 0.992834880956206 1.9692190039193191 5 0.8835909134723516 2.1906764808973014 6 0.6747421521061594 2.007456266131345 7 0.7486424830511197 2.5985406111589078 8 0.6586768627702985 2.612879584488417 9 0.581563473958166 2.595354172641239 >10 4.687851428204222 38.73912627855846 >50 0.012852231468688753 0.4365420769206258 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT 98 0.1561354873657713 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0015932192588344008 0.0 2 0.0 0.0 0.0 0.03505082369435682 0.0 3 0.0 0.0 0.0 0.03505082369435682 0.0 4 0.0 0.0 0.0 0.06213555109454163 0.0 5 0.0 0.0 0.0 0.07010164738871363 0.0 6 0.0 0.0 0.0 0.07010164738871363 0.0 7 0.0 0.0 0.0 0.07010164738871363 0.0 8 0.0 0.0 0.0 0.07169486664754804 0.0 9 0.0 0.0 0.0 0.11152534811840806 0.0 10 0.0 0.0 0.0 0.15932192588344007 0.0 11 0.0 0.0 0.0 0.16091514514227448 0.0 12 0.0 0.0 0.0 0.16250836440110888 0.0 13 0.0 0.0 0.0 0.1641015836599433 0.0 14 0.0 0.0 0.0 0.1832202147659561 0.0 15 0.0 0.0 0.0 0.1895930918012937 0.0 16 0.0 0.0 0.0 0.22464391549565052 0.0 17 0.0 0.0 0.0 0.23261001178982252 0.0 18 0.0 0.0 0.0 0.29155912436669534 0.0 19 0.0 0.0 0.0 0.3632539910142434 0.0 20 0.0 0.0 0.0 0.3998980339674346 0.0 21 0.0 0.0 0.0 0.4030844724851034 0.0 22 0.0 0.0 0.0 0.43813529617946023 0.0 23 0.0 0.0 0.0 0.45406748876780423 0.0 24 0.0 0.0 0.0 0.4779657776503202 0.0 25 0.0 0.0 0.0 0.5703724946627154 0.0 26 0.0 0.0 0.0 0.5735589331803843 0.0 27 0.0 0.0 0.0 0.5990504413217347 0.0 28 0.0 0.0 0.0 0.6675588694516139 0.0 29 0.0 0.0 0.0 0.7169486664754804 0.0 30 0.0 0.0 0.0 0.7615588057228436 0.0 31 0.0 0.0 0.0 0.81094860274671 0.0 32 0.0 0.0 0.0 0.8603383997705765 0.0 33 0.0 0.0 0.0 0.9527451167829717 0.0 34 0.0 0.0 0.0 1.097728069336902 0.0 35 0.0 0.0 0.0 1.2395245833731638 0.0 36 0.0 0.0 0.0 1.5725074084695536 0.0 37 0.0 0.0 0.0 2.106235860179078 0.0 38 0.0 0.0 0.0 2.568269445241054 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCTTC 20 7.7446556E-4 44.0631 19 CCCCACA 20 7.7446556E-4 44.0631 14 CTAAGGT 20 7.775215E-4 44.02793 38 TAAGGTC 20 7.775215E-4 44.02793 39 TTTACAC 30 1.294418E-4 36.631477 2 CATCTTC 25 0.002319481 35.25048 14 TTTCTCG 25 0.0023285984 35.222347 40 TGAAGTC 25 0.0023469182 35.166218 3 GAGACAT 25 0.0023469182 35.166218 3 AAGAGCG 30 0.005664221 29.375399 21 AGTAGAA 30 0.005664221 29.375399 16 GAGCTAA 30 0.0056864005 29.351955 35 GTTCATA 30 0.0056864005 29.351955 10 AGAGCTA 30 0.0056864005 29.351955 34 ACTATGC 30 0.0057309656 29.305182 8 GTTAGTT 30 0.0057309656 29.305182 43 GGGGTTC 30 0.0057309656 29.305182 7 GAAGTCC 30 0.0057309656 29.305182 4 GGGTTCA 30 0.0057309656 29.305182 8 ATTATAT 30 0.0057309656 29.305182 33 >>END_MODULE