FastQCFastQC Report
Wed 25 May 2016
SRR1780733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62766
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT1213819.338495363731955No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT40276.4158939553261325TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC17912.8534556925724117No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG16122.568269445241054No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC5160.8221011375585507No Hit
ATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTT3150.5018640665328362TruSeq Adapter, Index 10 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCT2990.47637255839148585No Hit
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC2460.3919319376732626No Hit
CATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCT1900.30271165917853615TruSeq Adapter, Index 10 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1900.30271165917853615No Hit
ACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCT1630.2596947391900073RNA PCR Primer, Index 10 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATATCGTATGCCGT1370.2182710384603129No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC990.15772870662460567No Hit
CAGTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTG790.12586432144791768No Hit
CTTATAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG660.10515247108307045No Hit
AAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCGATTAT630.10037281330656726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAT207.7996484E-444.00000416
CTACACG207.7996484E-444.0000044
CGACACG207.7996484E-444.0000049
ACACGTA207.7996484E-444.00000411
TGCACGC207.7996484E-444.00000412
ACACGAC207.7996484E-444.0000046
GTGCACG207.7996484E-444.00000411
AGCTTAT207.7996484E-444.0000042
TTGTAGC207.7996484E-444.00000423
AAATTAA207.7996484E-444.0000041
CACGTAC207.7996484E-444.00000412
ACGTACT207.7996484E-444.00000413
TACACGA207.7996484E-444.0000045
GACACGT207.7996484E-444.00000410
TACGTTG207.7996484E-444.00000419
CTACGTT207.7996484E-444.00000418
ACGACAC207.7996484E-444.0000048
ATGTACC207.7996484E-444.00000431
TACTACG207.7996484E-444.00000416
ACTATGT207.7996484E-444.00000438
Produced by FastQC (version 0.11.5)