FastQCFastQC Report
Wed 25 May 2016
SRR1780732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48955
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCAT200.0069169352.491576
CATCGAG200.0069169352.4915718
GGCTGCA200.0069169352.4915745
TTTTCGA200.0069169352.4915721
AGGTCGT200.0069169352.4915723
TCCTGAT500.007149783427.9955047
AACCAGT500.007149783427.99550423
GATCCAA500.007149783427.99550411
ACAGCTC657.8577775E-426.9187568
GAGCAGT657.8577775E-426.91875669
AGCAGTC657.8577775E-426.91875670
ACGAGCA1154.021886E-521.3009367
AGTTTTT850.003743401720.5849347
CGAGCAG1205.5827142E-520.41339168
GACGAGC1601.1414304E-619.6843470
GATGTGG950.00712146118.41809522
GTTTTTT950.00712146118.41809548
CCTAGCC1250.001732138516.7973043
CGACGAG1905.893846E-616.57628669
CCGACGA2203.8161993E-412.7252368
Produced by FastQC (version 0.11.5)