##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780732_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48955 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.709958124808498 34.0 31.0 34.0 12.0 34.0 2 31.269655806352773 34.0 32.0 34.0 23.0 34.0 3 31.507200490246145 34.0 34.0 34.0 23.0 34.0 4 31.847084056786844 34.0 34.0 34.0 23.0 34.0 5 31.80408538453682 34.0 34.0 34.0 23.0 34.0 6 34.90066387498723 38.0 36.0 38.0 27.0 38.0 7 34.26634664487795 38.0 35.0 38.0 24.0 38.0 8 33.03309161474824 37.0 33.0 38.0 21.0 38.0 9 33.90370748646716 37.0 34.0 38.0 22.0 38.0 10-11 34.208599734450004 37.5 34.5 38.0 23.5 38.0 12-13 34.4391073434787 38.0 35.0 38.0 24.5 38.0 14-15 34.19221734245736 38.0 34.0 38.0 23.5 38.0 16-17 34.09615973853539 38.0 34.0 38.0 23.0 38.0 18-19 34.03953630885507 38.0 34.0 38.0 22.0 38.0 20-21 34.08449596568278 38.0 34.0 38.0 23.0 38.0 22-23 34.04713512409356 38.0 34.5 38.0 22.5 38.0 24-25 34.496956388520076 38.0 35.5 38.0 24.5 38.0 26-27 34.50172607496681 38.0 36.0 38.0 24.0 38.0 28-29 34.63721785313042 38.0 36.0 38.0 25.0 38.0 30-31 34.5619140026555 38.0 36.0 38.0 24.5 38.0 32-33 34.221877234194665 38.0 35.0 38.0 23.0 38.0 34-35 34.2677663160045 38.0 35.0 38.0 24.0 38.0 36-37 34.251036666326215 38.0 34.5 38.0 23.5 38.0 38-39 34.63941374731897 38.0 36.0 38.0 25.5 38.0 40-41 34.1376978858135 38.0 34.5 38.0 23.0 38.0 42-43 34.45026044326422 38.0 36.0 38.0 24.0 38.0 44-45 33.518854049637426 37.0 34.0 38.0 21.5 38.0 46-47 34.25308957205597 37.5 34.5 38.0 24.0 38.0 48-49 34.07673373506282 37.0 34.0 38.0 23.5 38.0 50-51 33.942876110713925 37.0 34.0 38.0 23.0 38.0 52-53 33.91021346134205 37.0 34.0 38.0 23.0 38.0 54-55 33.67865386579512 37.0 34.0 38.0 22.0 38.0 56-57 33.722316413032374 37.0 34.0 38.0 22.0 38.0 58-59 33.62716780716985 37.0 34.0 38.0 22.0 38.0 60-61 33.70869165560208 37.0 34.0 38.0 22.5 38.0 62-63 33.70568889796752 37.0 34.0 38.0 22.5 38.0 64-65 33.78266775610254 37.0 34.0 38.0 23.0 38.0 66-67 33.70221632111122 37.0 34.0 38.0 22.5 38.0 68-69 33.194658359718105 37.0 32.5 38.0 17.0 38.0 70-71 33.21817996118885 37.0 33.0 38.0 21.0 38.0 72-73 33.35225206822592 37.0 33.0 38.0 21.0 38.0 74-75 32.87278112552344 37.0 31.5 38.0 20.0 38.0 76 32.111142886324174 36.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.1881755375223015 1101 2 -0.8482485002952629 1101 3 -0.39688703543063397 1101 4 -2.1271114265047473 1101 5 -0.5759075109079497 1101 6 -1.4164097219415694 1101 7 -1.357904456462272 1101 8 -0.9808864185792245 1101 9 -0.05912429420489218 1101 10-11 -0.987579347336542 1101 12-13 -1.3693015048452821 1101 14-15 -0.25126151226456983 1101 16-17 -1.0470194913447557 1101 18-19 0.35255394950663543 1101 20-21 -0.9378876590901868 1101 22-23 -0.3011188434725014 1101 24-25 -1.179216772940876 1101 26-27 -1.5943017832266406 1101 28-29 -0.5353689113786473 1101 30-31 -1.2990076796567251 1101 32-33 -0.4680616028313409 1101 34-35 -1.7342844669112623 1101 36-37 -0.9504874305929221 1101 38-39 -0.8397731068516308 1101 40-41 -0.2838247515589529 1101 42-43 -0.9027908602394916 1101 44-45 -0.007449812315648785 1101 46-47 0.2862954609495958 1101 48-49 -1.5690526925431243 1101 50-51 -1.4870404511889177 1101 52-53 -0.5887152738541843 1101 54-55 -1.6559815834389013 1101 56-57 -1.196005242543066 1101 58-59 -1.3544008765736208 1101 60-61 -0.6670485177853109 1101 62-63 -1.607655143950673 1101 64-65 -0.15039081340719207 1101 66-67 -1.3334206072916928 1101 68-69 0.7585835141439219 1101 70-71 -0.33302915476175343 1101 72-73 -0.7635175645030259 1101 74-75 -2.035182347225195 1101 76 -2.095955159731666 1102 1 0.8821707828240193 1102 2 0.0956908936441323 1102 3 -0.243640282183879 1102 4 0.2014600020666819 1102 5 0.8414085064080687 1102 6 0.6201703646385184 1102 7 1.5477232491654362 1102 8 1.4056936680008647 1102 9 0.8666332815526872 1102 10-11 -0.04331527807246971 1102 12-13 -4.1611846217284025 1102 14-15 -1.2946597806628475 1102 16-17 -2.789443733769005 1102 18-19 -1.2974460504933631 1102 20-21 -0.46656731276983976 1102 22-23 -0.9724391897928513 1102 24-25 0.7701338764097727 1102 26-27 -5.563024726949585 1102 28-29 -0.11264163865137533 1102 30-31 0.6496936190445766 1102 32-33 -7.068278053047788 1102 34-35 -0.3632887959155866 1102 36-37 0.6671532620477691 1102 38-39 0.6620667199881964 1102 40-41 0.3560020882678856 1102 42-43 0.23757710512846586 1102 44-45 -6.273358903224743 1102 46-47 -0.3803712057170756 1102 48-49 -0.06937736786780135 1102 50-51 -6.362473351621816 1102 52-53 -1.7179360530749648 1102 54-55 0.8525682001108805 1102 56-57 -0.8796632512010731 1102 58-59 0.15252553035278993 1102 60-61 0.756111655374859 1102 62-63 -4.905598866894401 1102 64-65 -1.1066678696842516 1102 66-67 0.13032398145288937 1102 68-69 -7.628104797544388 1102 70-71 -0.8117088084414021 1102 72-73 0.48182875584330276 1102 74-75 -6.861805723848576 1102 76 -1.9459551597316675 1103 1 -0.7056089073653098 1103 2 -0.5055139256329753 1103 3 -0.3024182512028126 1103 4 0.2795150795193493 1103 5 -0.3150458825764417 1103 6 -1.437402785103309 1103 7 -0.018455752555730953 1103 8 0.0945060604277117 1103 9 -0.4924028630256245 1103 10-11 -0.23780753280568945 1103 12-13 -2.0081295442757323 1103 14-15 -1.174134823692107 1103 16-17 -1.4460702397931016 1103 18-19 -1.0977472553126404 1103 20-21 -1.8004743695684624 1103 22-23 -1.4701586390183223 1103 24-25 -0.13937559002740585 1103 26-27 -1.2856581176552666 1103 28-29 -0.11053320491644314 1103 30-31 -0.4105903740707433 1103 32-33 -5.87546393945054 1103 34-35 -1.1364385377400268 1103 36-37 -0.36993795998321133 1103 38-39 -0.11706408896188947 1103 40-41 0.12097627071194239 1103 42-43 0.3932569674348372 1103 44-45 -5.715828782742815 1103 46-47 -0.3638049406568342 1103 48-49 -1.107114028797234 1103 50-51 -0.30485717262009615 1103 52-53 -0.9494765005792729 1103 54-55 0.232344447959413 1103 56-57 1.1976603288333507 1103 58-59 0.6854859434336902 1103 60-61 0.8662665607104927 1103 62-63 -0.05284499426101519 1103 64-65 0.35120648487684747 1103 66-67 -0.6433421114903055 1103 68-69 -3.375178807871407 1103 70-71 0.3646250986153916 1103 72-73 0.6497719572202314 1103 74-75 -2.330910715242723 1103 76 0.17512917761773394 1104 1 -2.269614931461696 1104 2 -0.229309106355867 1104 3 0.4474311463875509 1104 4 0.16663857349525557 1104 5 -0.49162720787764513 1104 6 -0.12000820679005386 1104 7 0.20308039202257788 1104 8 1.190515096572291 1104 9 -0.6896167184473114 1104 10-11 -0.17233313521533233 1104 12-13 -1.3647560502998317 1104 14-15 -0.22323120923427098 1104 16-17 -1.133193733769005 1104 18-19 0.5213039495066383 1104 20-21 0.4383434015158727 1104 22-23 -0.4398499040785637 1104 24-25 -0.04995540930451625 1104 26-27 0.03429670162184095 1104 28-29 -0.856391638651381 1104 30-31 -0.6123599523839971 1104 32-33 -0.8124744816192191 1104 34-35 -0.3324852244870229 1104 36-37 -0.49802530938080025 1104 38-39 -0.3075761371546619 1104 40-41 0.8287699454107411 1104 42-43 0.6598985336999021 1104 44-45 -3.3608589032247416 1104 46-47 -0.33974620571707703 1104 48-49 -0.22160951072493873 1104 50-51 0.8678837912353217 1104 52-53 -0.5188289102178274 1104 54-55 -0.9282353713176903 1104 56-57 0.2806046059417824 1104 58-59 0.28868624463850523 1104 60-61 -0.37112048748227977 1104 62-63 -0.14979529546582881 1104 64-65 0.1553857017443292 1104 66-67 0.30710969573861036 1104 68-69 -3.730336940401532 1104 70-71 -1.144298094155694 1104 72-73 0.7581680415575889 1104 74-75 -0.8698414381342872 1104 76 -0.8084551597316647 1105 1 -1.957114931461696 1105 2 -0.7855591063558691 1105 3 -0.783818853612452 1105 4 0.2103885734952513 1105 5 0.09587279212235345 1105 6 0.517491793209949 1105 7 1.2655803920225779 1105 8 0.3592650965722939 1105 9 0.11038328155268573 1105 10-11 0.9557918647846719 1105 12-13 -1.3491310502998317 1105 14-15 0.11114379076573044 1105 16-17 1.0355562662309978 1105 18-19 0.4650539495066326 1105 20-21 -0.6585315984841245 1105 22-23 0.16640009592143912 1105 24-25 -0.062455409304519094 1105 26-27 0.26242170162184664 1105 28-29 -0.7532666386513824 1105 30-31 0.4501400476160029 1105 32-33 1.078150518380781 1105 34-35 1.00813977551298 1105 36-37 0.6082246906192026 1105 38-39 0.4892988628453381 1105 40-41 0.09126994541073685 1105 42-43 0.24739853369990072 1105 44-45 1.5547660967752606 1105 46-47 0.9571287942829301 1105 48-49 -0.021609510724942993 1105 50-51 -0.09149120876467265 1105 52-53 -0.653203910217826 1105 54-55 -1.0751103713176917 1105 56-57 -0.5381453940582119 1105 58-59 -0.1863137553614962 1105 60-61 0.2570045125177174 1105 62-63 0.6658297045341683 1105 64-65 0.39288570174432635 1105 66-67 0.11960969573860325 1105 68-69 1.1509130595984658 1105 70-71 0.8432019058443103 1105 72-73 -0.013706958442419648 1105 74-75 -1.6042164381342872 1105 76 -0.7272051597316675 1106 1 -0.3611471895262106 1106 2 1.0344005710634896 1106 3 -0.4844236923221281 1106 4 0.3551466380113837 1106 5 0.34325182438042035 1106 6 -0.01738723904811934 1106 7 1.0179997468612925 1106 8 0.776200580443259 1106 9 0.9763107009075256 1106 10-11 0.5559934776878919 1106 12-13 0.5884697561517811 1106 14-15 0.46628491979798525 1106 16-17 0.3831369113922847 1106 18-19 -0.2934137924288507 1106 20-21 -0.4764751468712163 1106 22-23 0.033436386244012795 1106 24-25 0.7045808810180603 1106 26-27 0.04780476613797191 1106 28-29 -0.5182867999416985 1106 30-31 -0.23060592012593162 1106 32-33 1.0104085828969147 1106 34-35 0.7839462271258881 1106 36-37 1.3150795293288766 1106 38-39 0.37024644349050106 1106 40-41 -0.23201634491183398 1106 42-43 0.4032453078934424 1106 44-45 0.8048669032268734 1106 46-47 0.9317255684764731 1106 48-49 0.7724429086298912 1106 50-51 0.8505450815579039 1106 52-53 0.46605012204023666 1106 54-55 0.5214622093274741 1106 56-57 0.9637699285224315 1106 58-59 0.6103596317352782 1106 60-61 0.5277706415499779 1106 62-63 0.9432490593728815 1106 64-65 1.4204058630346452 1106 66-67 1.1252548570289278 1106 68-69 1.0854896725016943 1106 70-71 0.4332220671346292 1106 72-73 0.44738175123499957 1106 74-75 -1.3954462768439697 1106 76 -1.0378906436026334 1107 1 0.2876455475802189 1107 2 0.5022777199914366 1107 3 0.2654700685432374 1107 4 0.350433483674891 1107 5 0.5085599178708584 1107 6 0.8472447872219249 1107 7 0.8363887752561112 1107 8 1.9564207851950428 1107 9 0.2905853773610687 1107 10-11 -0.006745560365033043 1107 12-13 0.6870403568858023 1107 14-15 0.28081070693339427 1107 16-17 1.095286805153151 1107 18-19 1.3398293986084369 1107 20-21 1.5276585212763578 1107 22-23 0.16671820969389017 1107 24-25 0.7390603092583632 1107 26-27 1.204169456112865 1107 28-29 -0.011893135657366827 1107 30-31 0.24188780210702276 1107 32-33 1.062581656105337 1107 34-35 0.5156248054530934 1107 36-37 0.14901810379285507 1107 38-39 0.7421993119471395 1107 40-41 0.5987736879257142 1107 42-43 0.22093895286157306 1107 44-45 0.8118207374938251 1107 46-47 0.6098982553607684 1107 48-49 0.5334241719097861 1107 50-51 1.2359227133910125 1107 52-53 1.2187833652312818 1107 54-55 0.38323543706554375 1107 56-57 0.8815215221094448 1107 58-59 0.9539369931414967 1107 60-61 0.8572664885656209 1107 62-63 -0.26890083438798484 1107 64-65 1.0750900430616852 1107 66-67 1.587124665798484 1107 68-69 1.2568823709757169 1107 70-71 0.8435948699161671 1107 72-73 0.6793693888629733 1107 74-75 -0.42998365370315383 1107 76 -0.45523659685741436 1108 1 -0.995513624272153 1108 2 0.664971939395766 1108 3 0.6525782052110785 1108 4 0.41047027284165694 1108 5 0.5016325960439225 1108 6 0.9026633618373978 1108 7 1.5575738560748675 1108 8 1.243415423369676 1108 9 0.9725156344938597 1108 10-11 0.9606529758957834 1108 12-13 0.9349171523145543 1108 14-15 0.7854289214846801 1108 16-17 0.2677458087146505 1108 18-19 0.5984689821863753 1108 20-21 1.3441236302740478 1108 22-23 -0.03002555767333348 1108 24-25 -0.4079006707424213 1108 26-27 -0.32432257942390663 1108 28-29 0.6760838515446963 1108 30-31 0.6055526293153477 1108 32-33 1.3108302569428787 1108 34-35 0.7744796447940203 1108 36-37 0.13886194552116393 1108 38-39 1.0629303987930498 1108 40-41 0.7758900434499552 1108 42-43 0.9919655271639556 1108 44-45 0.6402031882785266 1108 46-47 0.9080274870933849 1108 48-49 1.3529615677064228 1108 50-51 1.0380635297974194 1108 52-53 1.2643001747494935 1108 54-55 1.2677409358718492 1108 56-57 1.6541135928698907 1108 58-59 -0.633515552747113 1108 60-61 1.4743656236288345 1108 62-63 0.8592937568217494 1108 64-65 1.2985842311560916 1108 66-67 1.040688127111153 1108 68-69 1.9960315236507569 1108 70-71 2.290485402576337 1108 72-73 1.095811015413794 1108 74-75 1.2709673853951173 1108 76 0.9040448402683339 1109 1 -1.7116330037508511 1109 2 -1.270574166596834 1109 3 -0.21205680541967808 1109 4 -0.8409668481914956 1109 5 -0.7668531114921038 1109 6 -0.575957001970778 1109 7 -1.2533955115918758 1109 8 -0.8874216504156607 1109 9 0.40518749842015467 1109 10-11 0.5302647563509311 1109 12-13 -0.21596086957693217 1109 14-15 -0.02353241405355533 1109 16-17 -1.6719738542509361 1109 18-19 -0.54654243603553 1109 20-21 -0.16493220089376592 1109 22-23 0.18044377062023642 1109 24-25 -0.08817077075029545 1109 26-27 -0.044694262233576865 1109 28-29 -0.7671597109405397 1109 30-31 -0.4617951933478537 1109 32-33 0.4016444942843975 1109 34-35 -0.4165590196677442 1109 36-37 -0.5807813334771836 1109 38-39 -0.2104375829377929 1109 40-41 0.20230157191677023 1109 42-43 -0.6519237554567212 1109 44-45 -0.6224552887669077 1109 46-47 -1.0445278322231033 1109 48-49 -0.9655477637369927 1109 50-51 0.2945404177413522 1109 52-53 0.4812463909869962 1109 54-55 -0.2345230219201042 1109 56-57 -0.4559541289979734 1109 58-59 -0.41391164692775817 1109 60-61 -0.9620466923015627 1109 62-63 -1.1642907773935391 1109 64-65 -0.6096368886171248 1109 66-67 -0.5228601837794642 1109 68-69 0.16397781863461347 1109 70-71 -0.3221218893364082 1109 72-73 -0.512878645189403 1109 74-75 1.1510169956006493 1109 76 -0.16824431635817305 1110 1 -0.37756947691624276 1110 2 0.48100557895881835 1110 3 0.1503157617721662 1110 4 -0.40158695098027053 1110 5 0.5907591557587217 1110 6 -0.05510436063620716 1110 7 1.1811398325820193 1110 8 2.1881112504184443 1110 9 -0.6144855995661942 1110 10-11 -0.608500093257291 1110 12-13 0.2705586350148579 1110 14-15 0.16890165789859424 1110 16-17 -0.28769548202075157 1110 18-19 0.526504998457682 1110 20-21 -0.20507880128132427 1110 22-23 -0.24808854044220396 1110 24-25 0.7657132969891904 1110 26-27 -1.0753623892872497 1110 28-29 -0.23554373655347405 1110 30-31 -0.5691125747616255 1110 32-33 1.1173113575416238 1110 34-35 0.25813977551297995 1110 36-37 0.3866337815282961 1110 38-39 0.4183854013068711 1110 40-41 0.6439141761799689 1110 42-43 0.3189457364970991 1110 44-45 1.4016410967752577 1110 46-47 0.7621113117654446 1110 48-49 0.9851431116526825 1110 50-51 -0.0016092157576821364 1110 52-53 0.3072637471248285 1110 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1.677321101975231 2116 48-49 1.381996258505822 2116 50-51 1.0246145604660981 2116 52-53 1.4095364743975622 2116 54-55 0.3253703979130762 2116 56-57 0.825556529018705 2116 58-59 0.1225804754077302 2116 60-61 -0.5309762567130463 2116 62-63 1.7792912429957042 2116 64-65 1.5234145478981702 2116 66-67 1.0032635418924514 2116 68-69 0.8184611365215488 2116 70-71 -1.969538478771078 2116 72-73 0.5540815030960502 2116 74-75 -1.8520529765958287 2116 76 -0.5959551597316661 2117 1 -0.05551918678084533 2117 2 0.34888238300583296 2117 3 0.07748433787691056 2117 4 -0.026047596717514665 2117 5 -0.804792101494666 2117 6 -0.12466246210919962 2117 7 -2.9296323739348686 2117 8 -2.1891391587468583 2117 9 0.3623779624037482 2117 10-11 -0.9217347309600115 2117 12-13 -0.4839049864700371 2117 14-15 -1.7301461028512932 2117 16-17 -0.18837990398176885 2117 18-19 -0.05436094411038539 2117 20-21 -0.10334542827136062 2117 22-23 -0.005341925355160981 2117 24-25 -0.47781445185771076 2117 26-27 0.7921424463026909 2117 28-29 0.2772519783698968 2117 30-31 0.23943526038195984 2117 32-33 0.49623562476376293 2117 34-35 -0.5344134159763811 2117 36-37 -1.8638497774659015 2117 38-39 0.38524301178150466 2117 40-41 -1.549621012036063 2117 42-43 -0.12321316842776042 2117 44-45 0.9069602457114243 2117 46-47 0.21803304960207015 2117 48-49 -0.7436174894483472 2117 50-51 0.6862348550651163 2117 52-53 0.10204874935664776 2117 54-55 -0.6433284564240722 2117 56-57 -0.4539033727816175 2117 58-59 -0.25446535110617674 2117 60-61 0.7515523848581509 2117 62-63 -0.22991497631689128 2117 64-65 -0.8932180216599264 2117 66-67 -1.3096456234103258 2117 68-69 -0.45925981274196204 2117 70-71 0.7993854164826075 2117 72-73 1.124457935174604 2117 74-75 -0.21119782111301078 2117 76 0.48915122324705607 2118 1 -0.4235127809240602 2118 2 0.33332530224628343 2118 3 -0.2607677783436344 2118 4 -0.12442325446173541 2118 5 0.16260666309009508 2118 6 0.2815374921346745 2118 7 0.17068791890429935 2118 8 -3.438853182997601 2118 9 -1.5183129550064507 2118 10-11 -0.1719635115594187 2118 12-13 0.28416868088297065 2118 14-15 -0.42941400493320003 2118 16-17 -0.7706265294679326 2118 18-19 -0.7966395988804607 2118 20-21 -3.0883031038604685 2118 22-23 -1.3966711406377001 2118 24-25 -0.29541911898193973 2118 26-27 0.31124562635302766 2118 28-29 -0.6752760472535329 2118 30-31 -0.25856290937324644 2118 32-33 0.3534193355850874 2118 34-35 1.1774946142226597 2118 36-37 -0.1182538040044534 2118 38-39 0.17024644349050533 2118 40-41 -1.7588980653419455 2118 42-43 -0.5881525415689168 2118 44-45 0.5468023870978342 2118 46-47 -0.26074754980309933 2118 48-49 -0.5135785967464486 2118 50-51 0.18817949016005286 2118 52-53 -0.5274982650565292 2118 54-55 -0.41832273690909005 2118 56-57 0.6175333693826488 2118 58-59 0.979176836036352 2118 60-61 -0.9862078530736795 2118 62-63 0.16152862926535505 2118 64-65 -0.38927155632018895 2118 66-67 -1.7037774010355875 2118 68-69 0.8887154789533085 2118 70-71 -0.5614015887793471 2118 72-73 -1.3106827648940325 2118 74-75 0.5325107124033437 2118 76 -1.762621826398334 2119 1 1.1145517352049694 2119 2 0.7690242269774643 2119 3 0.33493114638755017 2119 4 -0.19711142650474756 2119 5 -0.2128772078776464 2119 6 0.645408459876613 2119 7 -0.48608627464408727 2119 8 0.5675984299056225 2119 9 -0.14670005178064827 2119 10-11 0.3762085314513399 2119 12-13 0.05191061636683969 2119 14-15 -0.3782312092342721 2119 16-17 0.3805562662309967 2119 18-19 -0.055779383826696005 2119 20-21 -1.2222815984841233 2119 22-23 0.5349417625880974 2119 24-25 -0.7139137426378497 2119 26-27 0.4155467016218495 2119 28-29 0.6956916946819547 2119 30-31 -0.7267349523839997 2119 32-33 0.4714838517141189 2119 34-35 0.15147310884631082 2119 36-37 -0.17760864271412657 2119 38-39 -0.24674280382132707 2119 40-41 -0.3668550545892586 2119 42-43 -1.0838514663001035 2119 44-45 -0.8583589032247474 2119 46-47 -0.4087045390504116 2119 48-49 -0.3972345107249424 2119 50-51 0.3187171245686571 2119 52-53 -1.0700789102178234 2119 54-55 -1.8221937046510206 2119 56-57 -0.0375203940582125 2119 58-59 0.31681124463850097 2119 60-61 -0.5834121541489452 2119 62-63 -0.3075036287991608 2119 64-65 0.19309403507765666 2119 66-67 0.3846096957386038 2119 68-69 -0.5742952737348617 2119 70-71 -0.8740897608223577 2119 72-73 -1.3207902917757508 2119 74-75 0.07057522853237685 2119 76 0.5440448402683344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 6.0 13 59.0 14 184.0 15 340.0 16 500.0 17 551.0 18 518.0 19 483.0 20 404.0 21 353.0 22 328.0 23 334.0 24 335.0 25 394.0 26 476.0 27 587.0 28 726.0 29 830.0 30 1141.0 31 1453.0 32 1902.0 33 2720.0 34 3946.0 35 6275.0 36 11001.0 37 13109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.223840078973346 23.13055281342547 11.560134912800264 31.08547219480092 2 16.502910836482485 19.981615769584312 39.168624246757226 24.346849147175977 3 17.39556735777755 25.515269124706364 30.097027882749465 26.992135634766623 4 12.243897456848126 18.907159636400774 41.03155959554693 27.817383311204168 5 12.062097844959657 36.592789296292516 37.57736696966602 13.767745889081809 6 25.799203350015322 40.851802675926876 17.879685425390665 15.469308548667144 7 24.869778367888877 32.585027065672556 23.791236850168524 18.753957716270044 8 21.83229496476356 35.279338167705035 19.338167705035236 23.55019916249617 9 23.066081094883057 15.783883158002245 23.401082626902255 37.74895312021244 10-11 23.933203962823 25.923807578388313 27.67950158308651 22.463486875702177 12-13 22.60034725768563 24.15075068940864 26.885915636809315 26.36298641609642 14-15 21.187825554080277 25.58880604636912 24.447962414462264 28.775405985088348 16-17 22.046777652946584 27.77652946583597 26.203656419160453 23.97303646205699 18-19 21.327749974466347 27.116739863139617 27.00439178837708 24.55111837401695 20-21 20.569911142886323 27.802063119191097 26.48963333673782 25.13839240118476 22-23 22.467572260238995 27.351649474006738 26.82667756102543 23.354100704728832 24-25 21.91400265549995 27.979777346542743 26.422224491880296 23.68399550607701 26-27 20.370748646716372 28.895924828924525 27.083035440710855 23.65029108364825 28-29 21.68624246757226 26.89306505974875 29.08895924828925 22.331733224389748 30-31 21.677050352364414 27.23725870697579 28.06761311408436 23.018077826575425 32-33 20.027576345623533 28.89490348279032 27.49565927892963 23.581860892656522 34-35 21.734245735879888 27.789806965580638 27.325094474517414 23.15085282402206 36-37 21.867020733326527 27.515064855479523 27.093248902052906 23.524665509141048 38-39 21.45235420283934 27.638647737718316 27.921560616893064 22.98743744254928 40-41 21.689306505974876 26.514145643958738 28.349504647124913 23.447043202941476 42-43 21.057093248902053 27.0094985190481 28.05842099887652 23.87498723317332 44-45 20.482075375344706 27.0503523644163 28.198345419262587 24.26922684097641 46-47 20.887549790624043 26.74394852415484 28.620161372689207 23.74834031253192 48-49 20.112348074762536 26.87263813706465 29.154325400878356 23.860688387294456 50-51 20.465733837197426 26.398733530793585 28.514962720866098 24.620569911142887 52-53 19.625165968746806 26.898171790419774 28.792768869369826 24.68389337146359 54-55 19.180880400367684 27.3424573587989 28.55070983556327 24.925952405270145 56-57 19.502604432642222 26.994178327035034 28.971504442855682 24.531712797467062 58-59 19.634358083954652 27.15657236237361 28.084975998365845 25.124093555305894 60-61 19.406597896026963 26.639771218465942 28.4516392605454 25.5019916249617 62-63 19.663977121846592 26.54580737411909 27.951179654785008 25.83903584924931 64-65 20.15524461239914 26.877744867735675 27.466040241037685 25.500970278827495 66-67 20.044939229905015 26.967623327545702 27.33224389745685 25.65519354509243 68-69 19.75691962005924 26.57951179654785 27.231130630170565 26.432437953222347 70-71 20.78132979266673 26.538657951179655 26.70105198651823 25.978960269635383 72-73 21.199060361556533 26.08211622919007 25.98815238484322 26.730671024410174 74-75 20.725155755285467 26.42937391481973 26.341538147278115 26.50393218261669 76 21.47278112552344 25.793075273210093 25.91563680931468 26.81850679195179 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.5 16 3.5 17 4.0 18 5.5 19 9.5 20 11.5 21 11.0 22 14.5 23 34.5 24 55.5 25 60.0 26 81.5 27 148.5 28 218.0 29 242.0 30 317.0 31 437.0 32 620.5 33 759.0 34 876.0 35 1140.0 36 1399.0 37 1511.0 38 1729.5 39 2212.5 40 2684.5 41 3058.0 42 3224.0 43 3379.5 44 3606.0 45 3686.0 46 3695.0 47 3775.0 48 3668.5 49 3304.0 50 3126.0 51 2843.0 52 2316.5 53 1875.0 54 1677.0 55 1569.0 56 1269.0 57 887.5 58 698.0 59 583.5 60 377.0 61 238.5 62 192.0 63 151.5 64 94.0 65 67.5 66 50.0 67 42.0 68 38.0 69 25.5 70 17.5 71 18.0 72 16.0 73 12.5 74 9.0 75 7.0 76 6.0 77 6.0 78 5.5 79 4.0 80 4.5 81 3.0 82 0.5 83 0.0 84 1.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 1.0 98 1.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6761311408436319 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.62659585333469 #Duplication Level Percentage of deduplicated Percentage of total 1 72.44051116333725 40.29619037891942 2 11.526880141010576 12.824022061076498 3 5.750587544065805 9.596568276989071 4 3.3563454759106928 7.468082933306097 5 2.0380434782608696 5.668471044837095 6 1.362367802585194 4.547032989480135 7 0.9400705052878966 3.6605045449902973 8 0.6866921269095183 3.0558676335410073 9 0.4883960047003525 2.4451026452864877 >10 1.410105757931845 10.438157491573895 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016341538147278113 0.0 2 0.0 0.0 0.0 0.016341538147278113 0.0 3 0.0 0.0 0.0 0.016341538147278113 0.0 4 0.0 0.0 0.0 0.018384230415687878 0.0 5 0.0 0.0 0.0 0.018384230415687878 0.0 6 0.0 0.0 0.0 0.018384230415687878 0.0 7 0.0 0.0 0.0 0.018384230415687878 0.0 8 0.0 0.0 0.0 0.018384230415687878 0.0 9 0.0 0.0 0.0 0.018384230415687878 0.0 10 0.0 0.0 0.0 0.02451230722091717 0.0 11 0.0 0.0 0.0 0.03064038402614646 0.0 12 0.0 0.0 0.0 0.032683076294556225 0.0 13 0.0 0.0 0.0 0.032683076294556225 0.0 14 0.0 0.0 0.0 0.038811153099785514 0.0 15 0.0 0.0 0.0 0.04085384536819528 0.0 16 0.0 0.0 0.0 0.04085384536819528 0.0 17 0.0 0.0 0.0 0.04493922990501481 0.0 18 0.0 0.0 0.0 0.046981922173424576 0.0 19 0.0 0.0 0.0 0.0510673067102441 0.0 20 0.0 0.0 0.0 0.053109998978653865 0.0 21 0.0 0.0 0.0 0.053109998978653865 0.0 22 0.0 0.0 0.0 0.06128076805229292 0.0 23 0.0 0.0 0.0 0.06740884485752222 0.0 24 0.0 0.0 0.0 0.07353692166275151 0.0 25 0.0 0.0 0.0 0.07557961393116128 0.0 26 0.0 0.0 0.0 0.0796649984679808 0.0 27 0.0 0.0 0.0 0.09804922888366868 0.0 28 0.0 0.0 0.0 0.11439076703094679 0.0 29 0.0 0.0 0.0 0.12868961290981515 0.0 30 0.0 0.0 0.0 0.15115922786232255 0.0 31 0.0 0.0 0.0 0.181799611888469 0.0 32 0.0 0.0 0.0 0.2103973036462057 0.0 33 0.0 0.0 0.0 0.2553365335512205 0.0 34 0.0 0.0 0.0 0.3186599938719232 0.0 35 0.0 0.0 0.0 0.38198345419262586 0.0 36 0.0 0.0 0.0 0.5208865284444898 0.0 37 0.0 0.0 0.0 0.7905219078745787 0.0 38 0.0 0.0 0.0 1.0683280563783066 0.0 39 0.0 0.0 0.0 1.4278418956184251 0.0 40 0.0 0.0 0.0 1.8894903482790317 0.0 41 0.0 0.0 0.0 2.4777857215810437 0.0 42 0.0 0.0 0.0 3.012971095904402 0.0 43 0.0 0.0 0.0 3.652333775916658 0.0 44 0.0 0.0 0.0 4.15892145848228 0.0 45 0.0 0.0 0.0 4.900418751915024 0.0 46 0.0 0.0 0.0 5.619446430395261 0.0 47 0.0 0.0 0.0 6.246552956797059 0.0 48 0.0 0.0 0.0 6.961495250740476 0.0 49 0.0 0.0 0.0 7.6417117761209274 0.0 50 0.0 0.0 0.0 8.321928301501378 0.0 51 0.0 0.0 0.0 8.99805944234501 0.0 52 0.0 0.0 0.0 9.623123276478399 0.0 53 0.0 0.0 0.0 10.311510570932489 0.0 54 0.0 0.0 0.0 10.879379021550404 0.0 55 0.0 0.0 0.0 11.443162087631498 0.0 56 0.0 0.0 0.0 11.972219385149627 0.0 57 0.0 0.0 0.0 12.493105913594118 0.0 58 0.0 0.0 0.0 12.981309365744051 0.0 59 0.0 0.0 0.0 13.549177816361965 0.0 60 0.0 0.0 0.0 14.131345112858748 0.0 61 0.0 0.0 0.0 14.662445102645286 0.0 62 0.0 0.0 0.0 15.256868552752527 0.0 63 0.0 0.0 0.0 15.834950464712492 0.0 64 0.0 0.0 0.0 16.396690838525178 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACCAT 20 0.00691693 52.49157 6 CATCGAG 20 0.00691693 52.49157 18 GGCTGCA 20 0.00691693 52.49157 45 TTTTCGA 20 0.00691693 52.49157 21 AGGTCGT 20 0.00691693 52.49157 23 TCCTGAT 50 0.0071497834 27.995504 7 AACCAGT 50 0.0071497834 27.995504 23 GATCCAA 50 0.0071497834 27.995504 11 ACAGCTC 65 7.8577775E-4 26.918756 8 GAGCAGT 65 7.8577775E-4 26.918756 69 AGCAGTC 65 7.8577775E-4 26.918756 70 ACGAGCA 115 4.021886E-5 21.30093 67 AGTTTTT 85 0.0037434017 20.58493 47 CGAGCAG 120 5.5827142E-5 20.413391 68 GACGAGC 160 1.1414304E-6 19.68434 70 GATGTGG 95 0.007121461 18.418095 22 GTTTTTT 95 0.007121461 18.418095 48 CCTAGCC 125 0.0017321385 16.797304 3 CGACGAG 190 5.893846E-6 16.576286 69 CCGACGA 220 3.8161993E-4 12.72523 68 >>END_MODULE