FastQCFastQC Report
Wed 25 May 2016
SRR1780732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48955
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT4370.8926565212950668No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3220.657746910427944Illumina PCR Primer Index 9 (95% over 22bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC990.20222653457256665No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC490.10009192115207845No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTT150.002212165970.0000145
AGCTTGA150.002212165970.0000121
AAGGTGA150.002212165970.0000116
TATGACA150.002212165970.0000153
GGAAGGT150.002212165970.0000114
GGCTGCC150.002212165970.0000139
TGTTTGA200.0069125452.556
CATGGGT200.0069125452.516
GAGCTTG200.0069125452.520
TACTTAT200.0069125452.547
TGGAAGG200.0069125452.513
GATCCCG200.0069125452.561
GTGATTC200.0069125452.540
CCTAAGT200.0069125452.565
TTATCGG200.0069125452.525
TTATATG200.0069125452.550
CTTATAT200.0069125452.549
ACATGTC200.0069125452.557
TTTATGC200.0069125452.570
CATGTCT200.0069125452.558
Produced by FastQC (version 0.11.5)