##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780732_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48955 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45646001429885 34.0 34.0 34.0 31.0 34.0 2 33.17181084669595 34.0 34.0 34.0 32.0 34.0 3 33.37217853130426 34.0 34.0 34.0 33.0 34.0 4 33.30967214789092 34.0 34.0 34.0 33.0 34.0 5 33.34689000102134 34.0 34.0 34.0 33.0 34.0 6 37.01822081503421 38.0 37.0 38.0 35.0 38.0 7 36.67995097538556 38.0 37.0 38.0 34.0 38.0 8 36.610601572873044 38.0 37.0 38.0 34.0 38.0 9 36.456153610458585 38.0 37.0 38.0 34.0 38.0 10-11 36.085568379123686 38.0 36.5 38.0 32.5 38.0 12-13 35.98975589827393 38.0 37.0 38.0 32.0 38.0 14-15 36.303544071085696 38.0 37.0 38.0 34.0 38.0 16-17 36.43434787049331 38.0 37.0 38.0 34.0 38.0 18-19 36.43479726279236 38.0 37.0 38.0 34.0 38.0 20-21 36.14275354917782 38.0 37.0 38.0 33.0 38.0 22-23 36.16429373914819 38.0 37.0 38.0 33.0 38.0 24-25 35.436891022367476 37.5 35.5 38.0 29.0 38.0 26-27 35.67912368501685 38.0 36.0 38.0 31.5 38.0 28-29 35.52802573792258 38.0 36.0 38.0 31.0 38.0 30-31 35.17575324277398 37.5 35.5 38.0 29.0 38.0 32-33 35.72833214176284 38.0 36.5 38.0 31.5 38.0 34-35 35.7870289040956 38.0 36.5 38.0 32.5 38.0 36-37 35.92537023797365 38.0 37.0 38.0 32.5 38.0 38-39 35.57875600040854 38.0 36.5 38.0 30.5 38.0 40-41 35.00059238075784 37.0 35.5 38.0 29.0 38.0 42-43 35.435614339699725 37.5 36.0 38.0 29.5 38.0 44-45 35.65314063936268 38.0 36.0 38.0 31.5 38.0 46-47 35.9926156674497 38.0 37.0 38.0 33.0 38.0 48-49 35.73992442038607 38.0 36.5 38.0 31.5 38.0 50-51 35.927933816770505 38.0 37.0 38.0 32.5 38.0 52-53 35.81829230926361 38.0 36.5 38.0 31.5 38.0 54-55 35.980451434991316 38.0 37.0 38.0 33.0 38.0 56-57 36.082126442651415 38.0 37.0 38.0 33.5 38.0 58-59 36.15012766826678 38.0 37.0 38.0 34.0 38.0 60-61 35.77299560821162 38.0 36.5 38.0 32.5 38.0 62-63 36.083964865692984 38.0 37.0 38.0 33.5 38.0 64-65 36.06857317945051 38.0 37.0 38.0 33.5 38.0 66-67 35.402972117250535 38.0 36.0 38.0 31.0 38.0 68-69 34.77968542539067 37.5 34.5 38.0 26.5 38.0 70-71 34.740782351138805 37.0 35.0 38.0 27.0 38.0 72-73 34.42732100908998 37.0 34.5 38.0 24.5 38.0 74-75 35.173302012051884 37.5 35.5 38.0 29.0 38.0 76 34.6512511490144 37.0 34.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20102254713280843 1101 2 0.4815988813973533 1101 3 0.31405476009427247 1101 4 0.5457258807693606 1101 5 0.437850695207878 1101 6 -1.2001062291046196 1101 7 0.06925413583356743 1101 8 0.6992167146304027 1101 9 0.32436766166711806 1101 10-11 0.1407668161836213 1101 12-13 0.8747228591343443 1101 14-15 0.9755354910404392 1101 16-17 0.1996931813581071 1101 18-19 -0.34274031477846734 1101 20-21 -0.22186068187016872 1101 22-23 1.0393894092803109 1101 24-25 -2.031492463545 1101 26-27 -0.7979670408034423 1101 28-29 1.0987459684193155 1101 30-31 0.9496630338916816 1101 32-33 0.7895907274232599 1101 34-35 0.16160235619281593 1101 36-37 0.56846872460806 1101 38-39 0.7994118477813714 1101 40-41 1.3235678996007678 1101 42-43 0.7871283812210237 1101 44-45 0.5035876017135337 1101 46-47 0.816872562744507 1101 48-49 -1.8084560983054416 1101 50-51 -1.7089381037283147 1101 52-53 -1.7661974363454007 1101 54-55 -0.42521833054811253 1101 56-57 -0.7741842837332769 1101 58-59 -1.53051787210822 1101 60-61 -2.56604128098909 1101 62-63 -0.6866642505762499 1101 64-65 -1.9299001062343635 1101 66-67 -1.517695245022331 1101 68-69 -1.7954422849946496 1101 70-71 -1.0075839909488593 1101 72-73 -1.642512935454782 1101 74-75 -1.0359895490779536 1101 76 -0.9092026363137222 1102 1 -0.5840423879321293 1102 2 0.43592788572635754 1102 3 0.4856997817392923 1102 4 0.6221328071762855 1102 5 0.2908809982381797 1102 6 0.6150452860468931 1102 7 0.07531474189417509 1102 8 0.5403422557559452 1102 9 0.5685235058229594 1102 10-11 0.7411997166165207 1102 12-13 0.7409566253681064 1102 14-15 -0.4319320414270891 1102 16-17 -0.014484307819380149 1102 18-19 -0.5637359857741373 1102 20-21 0.7075765475670579 1102 22-23 0.6443677642586607 1102 24-25 -5.548050905103445 1102 26-27 -1.2486163914527992 1102 28-29 0.2532914229647716 1102 30-31 1.925853510082149 1102 32-33 0.6649154027479298 1102 34-35 0.3185287631192324 1102 36-37 -2.2832628771235406 1102 38-39 -0.4219084985389756 1102 40-41 0.767182618215486 1102 42-43 0.7562842253768665 1102 44-45 0.8401676882936187 1102 46-47 1.2917643376362804 1102 48-49 -2.686919301768647 1102 50-51 -0.44551819030840534 1102 52-53 -0.1620848822328398 1102 54-55 0.35183794650816935 1102 56-57 0.7840408244918251 1102 58-59 -0.07380791539826248 1102 60-61 -6.418855133802939 1102 62-63 -2.3591750730870658 1102 64-65 0.22204794571369035 1102 66-67 1.1728891705620796 1102 68-69 -1.5502041897565562 1102 70-71 -1.628038536403409 1102 72-73 -6.49132245926431 1102 74-75 -2.41423630232471 1102 76 -1.2405879176990027 1103 1 0.8524464244602967 1103 2 0.5844648908898691 1103 3 0.018143843701423634 1103 4 0.649413357950813 1103 5 0.36738702233456877 1103 6 0.6011898643601512 1103 7 0.8771219708098386 1103 8 0.9249377807129164 1103 9 0.24167324764740528 1103 10-11 0.5534630556870894 1103 12-13 -0.2237163522566803 1103 14-15 -0.11824873677992542 1103 16-17 0.44029194002914807 1103 18-19 0.9539920693033181 1103 20-21 -0.24737181732106706 1103 22-23 0.5210889346545287 1103 24-25 -2.3837651908177264 1103 26-27 0.1515126963268898 1103 28-29 0.4870263627238032 1103 30-31 1.3537794997551345 1103 32-33 1.0987364010267555 1103 34-35 0.6442602605374645 1103 36-37 -0.3940632213576194 1103 38-39 0.244829883560854 1103 40-41 -0.12175886199104724 1103 42-43 -0.3061082014733856 1103 44-45 1.4283776710819183 1103 46-47 1.2750689847963486 1103 48-49 -1.6145440866223453 1103 50-51 -0.12318600442199568 1103 52-53 0.1837843088170743 1103 54-55 -0.04510697603916469 1103 56-57 0.39836956803743817 1103 58-59 -0.17965989474421917 1103 60-61 -3.9210065967977776 1103 62-63 -1.3486759336722614 1103 64-65 0.39485345345895695 1103 66-67 -0.01454628554807158 1103 68-69 0.582067755165987 1103 70-71 0.33852772865683534 1103 72-73 -4.292613337061212 1103 74-75 -2.0144944778840923 1103 76 -3.625613735254941 1104 1 -0.09386381650355702 1104 2 0.019856457154929785 1104 3 0.2553426388821478 1104 4 -0.3421529071094298 1104 5 -0.4903690017618203 1104 6 0.14629528604689312 1104 7 0.36906474189417793 1104 8 0.6564136843273687 1104 9 0.9595949343943886 1104 10-11 -0.23290742624062233 1104 12-13 0.490063768225248 1104 14-15 0.6823536728586248 1104 16-17 0.44533712075205045 1104 18-19 0.846085442797289 1104 20-21 -0.01608416671865598 1104 22-23 0.4483856214015276 1104 24-25 0.7506098091822722 1104 26-27 1.3781693228329175 1104 28-29 1.5282914229647702 1104 30-31 1.5079963672250116 1104 32-33 0.8952725456050743 1104 34-35 0.5203144774049377 1104 36-37 1.4412906943050316 1104 38-39 1.5263057871753034 1104 40-41 2.3993254753583457 1104 42-43 0.6536056539482971 1104 44-45 1.1187391168650436 1104 46-47 1.3368536233505708 1104 48-49 0.24656284108849746 1104 50-51 0.7477853811201669 1104 52-53 0.43077226062430185 1104 54-55 0.26076651793673733 1104 56-57 0.47913011020611407 1104 58-59 -0.5068436296839778 1104 60-61 0.7565912947684907 1104 62-63 0.666271355484362 1104 64-65 0.9354408028565402 1104 66-67 0.517532027704938 1104 68-69 1.9055993816720118 1104 70-71 1.821961463596594 1104 72-73 1.2970703978785494 1104 74-75 0.40406726910386226 1104 76 0.060304939443852845 1105 1 1.2686361834964401 1105 2 0.5886064571549312 1105 3 0.23034263888214923 1105 4 -0.004652907109431226 1105 5 0.2971309982381811 1105 6 0.4462952860468974 1105 7 1.106564741894175 1105 8 0.14391368432737295 1105 9 0.9720949343943914 1105 10-11 0.8733425737593805 1105 12-13 -0.416186231774752 1105 14-15 0.48235367285862907 1105 16-17 0.16408712075205045 1105 18-19 0.7304604427972876 1105 20-21 0.4557908332813412 1105 22-23 0.4390106214015219 1105 24-25 0.7506098091822722 1105 26-27 0.48129432283291607 1105 28-29 0.9626664229647659 1105 30-31 1.5111213672250088 1105 32-33 0.8858975456050757 1105 34-35 -0.37656052259505657 1105 36-37 0.728790694305026 1105 38-39 0.032555787175311934 1105 40-41 0.7618254753583429 1105 42-43 0.49735565394829706 1105 44-45 1.215614116865055 1105 46-47 1.2399786233505665 1105 48-49 0.0028128410884988853 1105 50-51 1.1509103811201697 1105 52-53 0.8588972606243033 1105 54-55 0.8201415179367402 1105 56-57 0.7135051102061141 1105 58-59 0.6869063703160165 1105 60-61 1.150341294768488 1105 62-63 -0.193103644515638 1105 64-65 1.0479408028565445 1105 66-67 0.6800320277049323 1105 68-69 0.48059938167201466 1105 70-71 1.150086463596594 1105 72-73 1.5939453978785494 1105 74-75 0.072817269103858 1105 76 0.8665549394438585 1106 1 -1.7932589777938794 1106 2 0.3299371023162223 1106 3 0.479939413075698 1106 4 0.4159116090196022 1106 5 0.4505584175930224 1106 6 0.6327872215307693 1106 7 -1.181136871009052 1106 8 0.6447201359402754 1106 9 -0.9061308720572185 1106 10-11 0.26205225117873 1106 12-13 1.3749428004833177 1106 14-15 -0.2724850368188001 1106 16-17 0.39392583042946683 1106 18-19 0.7551580234424549 1106 20-21 0.07958115586199455 1106 22-23 0.4216719117241041 1106 24-25 -0.39344261017257054 1106 26-27 0.8793790002522712 1106 28-29 0.5081301326421865 1106 30-31 0.7660608833540437 1106 32-33 0.45132093270184725 1106 34-35 0.9441047999855812 1106 36-37 1.2599398878534132 1106 38-39 0.4307412710462728 1106 40-41 1.481583539874471 1106 42-43 0.8106621055611996 1106 44-45 1.202610084606988 1106 46-47 1.3274786233505722 1106 48-49 1.6752926797981758 1106 50-51 -0.08779929629918115 1106 52-53 0.5614174219146193 1106 54-55 0.3353632921302818 1106 56-57 0.3528196263351475 1106 58-59 0.5812612090256977 1106 60-61 1.2081033915426858 1106 62-63 1.0064931296779065 1106 64-65 -0.765970487466042 1106 66-67 0.48487073738235154 1106 68-69 1.2938050268333043 1106 70-71 0.9308324313385299 1106 72-73 1.3174333011043515 1106 74-75 -0.07345289218646656 1106 76 0.6681678426696607 1107 1 1.0724161236162004 1107 2 0.2744223254184064 1107 3 0.1479324592414315 1107 4 0.4464698473815929 1107 5 0.03429417189087758 1107 6 0.6279195375439031 1107 7 0.9223207299181269 1107 8 0.25723703762078287 1107 9 0.010268587089001358 1107 10-11 1.0173545498072798 1107 12-13 0.4582149658300452 1107 14-15 0.6685812177688106 1107 16-17 0.764835623746059 1107 18-19 1.224715682318248 1107 20-21 0.9841590967543965 1107 22-23 0.23279804655122405 1107 24-25 0.7749174439128126 1107 26-27 0.6173160294197402 1107 28-29 1.487423159491712 1107 30-31 1.099613133692074 1107 32-33 1.4805270366230374 1107 34-35 0.5193414235127278 1107 36-37 1.5044830595744898 1107 38-39 1.1145542901693233 1107 40-41 1.1972296669751117 1107 42-43 1.5650951749063822 1107 44-45 1.2732301348291202 1107 46-47 1.3939456892188389 1107 48-49 1.097255206357957 1107 50-51 1.3694732553716662 1107 52-53 1.195742320504543 1107 54-55 1.1770090329067955 1107 56-57 1.1504437329606034 1107 58-59 1.3511279272022492 1107 60-61 1.3016324624331546 1107 62-63 1.1439472536879478 1107 64-65 1.4193105633355856 1107 66-67 0.4174571774055309 1107 68-69 1.8502662978396742 1107 70-71 0.8421710444349202 1107 72-73 1.6197126134474118 1107 74-75 0.032940772097873605 1107 76 2.063261526270203 1108 1 -0.17731969885650045 1108 2 0.36944796042290307 1108 3 -0.12415082517014042 1108 4 0.5944484000801111 1108 5 0.06767838385909641 1108 6 -0.3990070015348053 1108 7 -0.1694564999358903 1108 8 -1.358864093450407 1108 9 -0.9061730394618195 1108 10-11 0.42203538421689757 1108 12-13 1.387571938159887 1108 14-15 0.7660138035775859 1108 16-17 -0.5177756243459939 1108 18-19 0.5537243316861762 1108 20-21 1.0097941012552027 1108 22-23 0.5889289220551177 1108 24-25 1.2763655281365232 1108 26-27 -0.2946247948141405 1108 28-29 -1.217950407100595 1108 30-31 0.3883885240877589 1108 32-33 0.49175947370964934 1108 34-35 0.7958863728297771 1108 36-37 1.12721798188673 1108 38-39 1.6489365061295587 1108 40-41 1.3780836452929819 1108 42-43 0.39727395460189285 1108 44-45 1.1102423848389051 1108 46-47 1.1601583619126643 1108 48-49 1.4487482986048335 1108 50-51 1.0430672438652664 1108 52-53 0.21018402533018588 1108 54-55 -0.09225635788025954 1108 56-57 0.027434848768209008 1108 58-59 0.5632135598585037 1108 60-61 0.9590626999972471 1108 62-63 1.1252787084255331 1108 64-65 0.596837861680072 1108 66-67 0.9862411780317331 1108 68-69 1.7535773228484857 1108 70-71 0.009052966864572909 1108 72-73 -0.09104234721949211 1108 74-75 -0.27581426684385235 1108 76 1.3969470963065973 1109 1 -1.4668306839734377 1109 2 -0.0962580006763929 1109 3 0.3976619159905823 1109 4 -0.11564688301304216 1109 5 -0.024179242725672623 1109 6 -0.5192920633506972 1109 7 -0.1831189930455821 1109 8 0.2683112746888199 1109 9 0.9585407175269225 1109 10-11 0.8878004050846755 1109 12-13 0.7792956959360922 1109 14-15 0.7492211427381434 1109 16-17 0.5336654340050515 1109 18-19 0.5594137560502972 1109 20-21 0.8670860140042436 1109 22-23 0.3825347177870668 1109 24-25 -0.025331455877967812 1109 26-27 0.19970546741123485 1109 28-29 -1.320202552938845 1109 30-31 0.13691202987561724 1109 32-33 1.005362907050852 1109 34-35 -0.12983612500470088 1109 36-37 1.018587381052022 1109 38-39 1.127360004042778 1109 40-41 0.11318089704509049 1109 42-43 1.1547351720205867 1109 44-45 1.1121126108409527 1109 46-47 0.6063942860011764 1109 48-49 0.8975041061487374 1109 50-51 0.009344116059928353 1109 52-53 -0.35838436588172584 1109 54-55 -0.5029383013403717 1109 56-57 -0.2763292271432789 1109 58-59 0.22094251489433958 1109 60-61 0.892246415250419 1109 62-63 0.40734816271327645 1109 64-65 0.798467911290281 1109 66-67 0.4734055216808386 1109 68-69 -0.32154670266533714 1109 70-71 0.7240698973315318 1109 72-73 -0.8317699635672327 1109 74-75 -0.9120848393298786 1109 76 0.27197662619084184 1110 1 -0.930096333986075 1110 2 -0.3325036827052088 1110 3 0.30123424727375436 1110 4 0.002602337645818409 1110 5 0.649971907329089 1110 6 -0.2566330356314239 1110 7 0.6840560006354366 1110 8 0.6566759220896117 1110 9 0.8068851441845979 1110 10-11 -0.9521819017650941 1110 12-13 0.354617964029444 1110 14-15 0.6984375889425465 1110 16-17 0.6355906172555379 1110 18-19 0.22077949874134362 1110 20-21 0.9252838402743535 1110 22-23 -0.4521607072698046 1110 24-25 -0.5900588971114331 1110 26-27 1.3917182738818639 1110 28-29 0.5008438705172225 1110 30-31 0.40100336023200356 1110 32-33 1.137492825325353 1110 34-35 1.0695277641182273 1110 36-37 1.0827461138854488 1110 38-39 1.5272673256368492 1110 40-41 -0.20382137778850762 1110 42-43 0.10443607352870998 1110 44-45 0.5851726832986159 1110 46-47 0.2918142876862362 1110 48-49 1.4902035753542293 1110 50-51 -0.02041829020850372 1110 52-53 0.5653876452396887 1110 54-55 -0.5321530624828412 1110 56-57 1.0297201451711544 1110 58-59 0.05980846821811525 1110 60-61 0.42055545560764784 1110 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50-51 -2.1519742342644435 2116 52-53 -1.4394200470680047 2116 54-55 -0.9565411743709546 2116 56-57 -0.7744756590246524 2116 58-59 -3.240016706607058 2116 60-61 -3.2547067821545923 2116 62-63 -0.37868056759256064 2116 64-65 -1.9631168894511504 2116 66-67 -1.0141987415258313 2116 68-69 -1.2537756183279853 2116 70-71 0.6008076174427472 2116 72-73 -0.28033344827530016 2116 74-75 -0.09761542320383398 2116 76 -1.9488296759407575 2117 1 0.6724925664751638 2117 2 0.6371436911974868 2117 3 0.38433200058427275 2117 4 -0.14640822625836591 2117 5 0.10258312589775898 2117 6 0.299087839238382 2117 7 0.44659133763885706 2117 8 0.2593392162422674 2117 9 -1.5305646400736919 2117 10-11 -1.3011255113470028 2117 12-13 -2.1124628275194297 2117 14-15 -0.4889229228860543 2117 16-17 -1.2066575600990177 2117 18-19 -1.7606964720963276 2117 20-21 -0.06043257097397259 2117 22-23 -0.5437021445559225 2117 24-25 -2.5858928503921987 2117 26-27 -3.6337987622734644 2117 28-29 -1.8935170876735299 2117 30-31 -1.2834929944771147 2117 32-33 -2.25924873099067 2117 34-35 -0.44590892685037886 2117 36-37 -2.2304513269715684 2117 38-39 -0.3569388936757534 2117 40-41 -0.7762064395352652 2117 42-43 -0.9587613673283002 2117 44-45 -1.1621119469647425 2117 46-47 -1.574649036223903 2117 48-49 -1.4993813078476705 2117 50-51 -0.83845132100749 2117 52-53 0.29779353722004487 2117 54-55 -1.7017334820632612 2117 56-57 -1.4680108472406914 2117 58-59 -2.474263842449936 2117 60-61 -1.506241152040026 2117 62-63 -1.873156836005002 2117 64-65 -1.9091070694838805 2117 66-67 -1.838053078678037 2117 68-69 -1.017073490668409 2117 70-71 -2.510485344914045 2117 72-73 0.14028848298492846 2117 74-75 0.1199582265506649 2117 76 0.161235790507682 2118 1 -1.3696030638153864 2118 2 -0.03135322026442111 2118 3 -1.016834780472692 2118 4 -0.4679593587223323 2118 5 0.3322788477005503 2118 6 0.08655066239098375 2118 7 0.8339168924318088 2118 8 0.1931072327144676 2118 9 -1.7792491516271127 2118 10-11 -1.0142918348427727 2118 12-13 -1.5884980597317409 2118 14-15 0.2232138879123866 2118 16-17 -3.2372569652694594 2118 18-19 -1.4426914389231413 2118 20-21 -1.103214543062741 2118 22-23 0.043177288068186215 2118 24-25 -0.0450555133983741 2118 26-27 -4.123846806199342 2118 28-29 -0.8929451361750154 2118 30-31 -1.6135090091190705 2118 32-33 -1.2712597124594396 2118 34-35 -1.0612715441004354 2118 36-37 -4.223931079888523 2118 38-39 -0.5972157182010349 2118 40-41 -1.6033626966846626 2118 42-43 0.27087715932464107 2118 44-45 -3.642551205715595 2118 46-47 0.11672593517852903 2118 48-49 -0.7010514062233284 2118 50-51 -1.8437132747938136 2118 52-53 -1.35686214797785 2118 54-55 -1.0054969229234771 2118 56-57 0.4194862930018104 2118 58-59 -0.7208893286087132 2118 60-61 -0.7725417697476402 2118 62-63 -1.666625149891992 2118 64-65 -0.8595188745628093 2118 66-67 -0.9828711981015132 2118 68-69 -1.6449046505860494 2118 70-71 -0.6444363858657738 2118 72-73 1.1142074946527387 2118 74-75 -0.5390442900359318 2118 76 -0.3898966734593685 2119 1 0.5073861834964433 2119 2 -0.9126435428450677 2119 3 -0.1604906944511839 2119 4 -0.815486240442759 2119 5 -0.6724523350951515 2119 6 -1.0282880472864377 2119 7 -1.108018591439155 2119 8 0.12558035099403497 2119 9 -1.1229050656056074 2119 10-11 -0.6033240929072861 2119 12-13 -1.0145195651080812 2119 14-15 -0.5243129938080386 2119 16-17 -0.5875795459146218 2119 18-19 -1.0484978905360478 2119 20-21 -2.041709166718647 2119 22-23 -1.0768227119318112 2119 24-25 -0.15043185748439214 2119 26-27 0.0340026561662512 2119 28-29 0.286624756298103 2119 30-31 0.7746630338916773 2119 32-33 -0.9022274543949322 2119 34-35 -0.49718552259506055 2119 36-37 -0.11683430569497233 2119 38-39 -2.6445275461580238 2119 40-41 -2.070674524641653 2119 42-43 -0.5684776793850332 2119 44-45 -0.07459421646828446 2119 46-47 -0.48585470998276037 2119 48-49 -1.2040621589115048 2119 50-51 0.6175770477868383 2119 52-53 -0.7458944060423676 2119 54-55 -0.9984001487299281 2119 56-57 -2.091911556460552 2119 58-59 -1.3514269630173104 2119 60-61 0.2663829614351556 2119 62-63 -0.5406036445156417 2119 64-65 -0.10080919714345526 2119 66-67 0.29669869437160656 2119 68-69 -0.9421089516613179 2119 70-71 -0.5863718697367375 2119 72-73 0.17915373121187628 2119 74-75 -1.6728077308961389 2119 76 -0.5834450605561443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 8.0 23 13.0 24 21.0 25 67.0 26 100.0 27 166.0 28 302.0 29 505.0 30 881.0 31 1444.0 32 2180.0 33 3461.0 34 5098.0 35 7579.0 36 12300.0 37 14830.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.24869778367889 24.038402614646103 12.325605147584517 28.38729445409049 2 17.530385047492594 19.583290777244407 39.522010009192115 23.364314166070884 3 16.670411602492084 26.51618833622715 30.536206720457564 26.277193340823207 4 12.29700745582678 19.90603615565315 39.681340006128075 28.115616382391995 5 11.69237054437749 38.86630579103258 36.809314676743945 12.63200898784598 6 26.04024103768767 40.962108058420995 18.32907772444081 14.668573179450517 7 25.8155448881626 31.102032478807068 24.52252068225922 18.559901950771117 8 21.895618425084262 37.06669390256358 20.68634460218568 20.35134307016648 9 22.992544173220306 15.24665509141048 23.627821468695743 38.13297926667348 10-11 24.099683382698398 26.625472372587073 28.147278112552343 21.12756613216219 12-13 23.30099070575018 23.9536308855071 26.718414870799712 26.02696353794301 14-15 21.06526401797569 25.68276989071596 24.02716780716985 29.224798284138494 16-17 22.216321111224595 28.29026657134103 26.105607190276785 23.387805127157595 18-19 21.753651312429785 27.68971504442856 27.022776018792772 23.53385762434889 20-21 21.024410172607496 27.558982739250332 26.739863139618013 24.676743948524155 22-23 21.78327035032172 28.40874272290879 27.08405678684506 22.72393013992442 24-25 21.728117659074663 29.036870595444796 26.68573179450516 22.549279950975386 26-27 21.481973240731282 28.506791951792465 27.604943315289553 22.406291492186703 28-29 21.948728424062917 27.587580431008067 28.712082524767645 21.751608620161374 30-31 21.05198651823103 27.524256970687368 28.724338678378103 22.699417832703503 32-33 20.664896333367377 29.976509038913292 27.211725053620672 22.146869574098663 34-35 21.48707997140231 28.831580022469616 27.586559084873862 22.094780921254213 36-37 22.29292207128996 27.388417934838117 27.41190889592483 22.906751097947094 38-39 20.98151363497089 27.63354100704729 28.17587580431008 23.20906955367174 40-41 20.689408640588294 27.172913900520886 29.119599632315392 23.018077826575425 42-43 21.908895924828926 26.991114288632417 28.962312327647837 22.137677458890817 44-45 20.128689612909813 27.07690736390563 29.20743539985701 23.586967623327546 46-47 21.649474006740885 26.351751608620162 28.77234194668573 23.226432437953225 48-49 20.723113063017056 26.145439689510773 29.317740782351137 23.81370646512103 50-51 20.1675007660096 26.1719946890001 29.4137473189664 24.2467572260239 52-53 19.889694617505874 26.10458584414258 31.02951690327852 22.976202635073026 54-55 19.040955979981618 27.003370442242876 29.787560004085385 24.16811357369012 56-57 19.92748442447145 26.388520069451538 29.36574405065877 24.318251455418242 58-59 19.04402001838423 27.803084465325302 29.368808089061382 23.78408742722909 60-61 19.86824634868757 28.171790419773263 27.941987539577163 24.017975691962008 62-63 18.403635992237767 28.875497906240426 27.84087427229088 24.879991829230928 64-65 19.197221938514964 29.49341231743438 26.525380451434994 24.783985292615668 66-67 19.320804820753754 29.317740782351137 26.005515269124707 25.355939127770398 68-69 19.049126749055254 29.13083443979165 26.53763660504545 25.28240220610765 70-71 19.93565519354509 28.840772137677455 26.047390460627106 25.176182208150344 72-73 19.6231232764784 28.0543356143397 25.726687774486773 26.59585333469513 74-75 19.795730773159022 28.327035032172404 26.00245123072209 25.87478296394648 76 19.73445000510673 28.46900214482688 26.18527218874477 25.61127566132162 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 1.0 16 4.0 17 6.0 18 3.5 19 8.5 20 17.0 21 18.0 22 12.5 23 20.0 24 40.5 25 48.0 26 89.0 27 166.0 28 245.5 29 289.0 30 357.0 31 465.0 32 674.0 33 843.0 34 933.0 35 1143.5 36 1405.5 37 1547.0 38 1822.5 39 2374.0 40 3070.5 41 3596.0 42 3701.0 43 3721.5 44 3871.5 45 3992.5 46 3984.0 47 3947.0 48 3769.5 49 3261.0 50 2893.0 51 2699.0 52 2163.0 53 1640.0 54 1459.0 55 1334.0 56 955.0 57 580.5 58 460.0 59 316.5 60 137.5 61 72.0 62 42.0 63 25.0 64 9.0 65 6.5 66 2.0 67 1.0 68 1.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48955.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.52139720151159 #Duplication Level Percentage of deduplicated Percentage of total 1 63.35203051379852 28.83872944540905 2 14.476105003365491 13.179450515779797 3 7.763069329145165 10.601572873046676 4 4.35719093560691 7.933816770503524 5 2.7327798967915635 6.219997957307732 6 1.8936504375140228 5.172096823613523 7 1.3327350235584474 4.246757226023899 8 0.9513125420686561 3.46440608722296 9 0.668611173435046 2.7392503319374937 >10 2.4590531747812427 15.851292002859768 >50 0.004487323311644604 0.20222653457256665 >100 0.008974646623289208 1.550403431723011 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 437 0.8926565212950668 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 322 0.657746910427944 Illumina PCR Primer Index 9 (95% over 22bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 99 0.20222653457256665 No Hit CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 49 0.10009192115207845 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01021346134204882 0.0 2 0.0 0.0 0.0 0.012256153610458585 0.0 3 0.0 0.0 0.0 0.012256153610458585 0.0 4 0.0 0.0 0.0 0.014298845878868349 0.0 5 0.0 0.0 0.0 0.014298845878868349 0.0 6 0.0 0.0 0.0 0.014298845878868349 0.0 7 0.0 0.0 0.0 0.014298845878868349 0.0 8 0.0 0.0 0.0 0.014298845878868349 0.0 9 0.0 0.0 0.0 0.014298845878868349 0.0 10 0.0 0.0 0.0 0.02042692268409764 0.0 11 0.0 0.0 0.0 0.026554999489326932 0.0 12 0.0 0.0 0.0 0.028597691757736698 0.0 13 0.0 0.0 0.0 0.028597691757736698 0.0 14 0.0 0.0 0.0 0.032683076294556225 0.0 15 0.0 0.0 0.0 0.03472576856296599 0.0 16 0.0 0.0 0.0 0.03472576856296599 0.0 17 0.0 0.0 0.0 0.03472576856296599 0.0 18 0.0 0.0 0.0 0.036768460831375756 0.0 19 0.0 0.0 0.0 0.04085384536819528 0.0 20 0.0 0.0 0.0 0.04085384536819528 0.0 21 0.0 0.0 0.0 0.04085384536819528 0.0 22 0.0 0.0 0.0 0.046981922173424576 0.0 23 0.0 0.0 0.0 0.057195383515473396 0.0 24 0.0 0.0 0.0 0.05923807578388316 0.0 25 0.0 0.0 0.0 0.06128076805229292 0.0 26 0.0 0.0 0.0 0.06536615258911245 0.0 27 0.0 0.0 0.0 0.0796649984679808 0.0 28 0.0 0.0 0.0 0.09804922888366868 0.0 29 0.0 0.0 0.0 0.10621999795730773 0.0 30 0.0 0.0 0.0 0.12664692064140537 0.0 31 0.0 0.0 0.0 0.15728730466755184 0.0 32 0.0 0.0 0.0 0.1879276886936983 0.0 33 0.0 0.0 0.0 0.2328669185987131 0.0 34 0.0 0.0 0.0 0.285976917577367 0.0 35 0.0 0.0 0.0 0.34930037789806967 0.0 36 0.0 0.0 0.0 0.4779899908078848 0.0 37 0.0 0.0 0.0 0.7496680625063834 0.0 38 0.0 0.0 0.0 1.0254315187417016 0.0 39 0.0 0.0 0.0 1.3747318966397712 0.0 40 0.0 0.0 0.0 1.8200388111530998 0.0 41 0.0 0.0 0.0 2.418547645797161 0.0 42 0.0 0.0 0.0 2.961903789194158 0.0 43 0.0 0.0 0.0 3.595138392401185 0.0 44 0.0 0.0 0.0 4.120110305382494 0.0 45 0.0 0.0 0.0 4.84730875293637 0.0 46 0.0 0.0 0.0 5.556122970074559 0.0 47 0.0 0.0 0.0 6.2036564191604535 0.0 48 0.0 0.0 0.0 6.914513328567051 0.0 49 0.0 0.0 0.0 7.62332754570524 0.0 50 0.0 0.0 0.0 8.293330609743641 0.0 51 0.0 0.0 0.0 8.965376366050455 0.0 52 0.0 0.0 0.0 9.594525584720662 0.0 53 0.0 0.0 0.0 10.280870186906343 0.0 54 0.0 0.0 0.0 10.863037483403126 0.0 55 0.0 0.0 0.0 11.43090593402104 0.0 56 0.0 0.0 0.0 11.964048616075988 0.0 57 0.0 0.0 0.0 12.515575528546625 0.0 58 0.0 0.0 0.0 13.013992442038607 0.0 59 0.0 0.0 0.0 13.58594627719334 0.0 60 0.0 0.0 0.0 14.155857420079665 0.0 61 0.0 0.0 0.0 14.678786640792564 0.0 62 0.0 0.0 0.0 15.252783168215709 0.0 63 0.0 0.0 0.0 15.84312123378613 0.0 64 0.0 0.0 0.0 16.37422122357267 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACTT 15 0.0022121659 70.00001 45 AGCTTGA 15 0.0022121659 70.00001 21 AAGGTGA 15 0.0022121659 70.00001 16 TATGACA 15 0.0022121659 70.00001 53 GGAAGGT 15 0.0022121659 70.00001 14 GGCTGCC 15 0.0022121659 70.00001 39 TGTTTGA 20 0.00691254 52.5 56 CATGGGT 20 0.00691254 52.5 16 GAGCTTG 20 0.00691254 52.5 20 TACTTAT 20 0.00691254 52.5 47 TGGAAGG 20 0.00691254 52.5 13 GATCCCG 20 0.00691254 52.5 61 GTGATTC 20 0.00691254 52.5 40 CCTAAGT 20 0.00691254 52.5 65 TTATCGG 20 0.00691254 52.5 25 TTATATG 20 0.00691254 52.5 50 CTTATAT 20 0.00691254 52.5 49 ACATGTC 20 0.00691254 52.5 57 TTTATGC 20 0.00691254 52.5 70 CATGTCT 20 0.00691254 52.5 58 >>END_MODULE