FastQCFastQC Report
Wed 25 May 2016
SRR1780731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515454
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAT250.00234123635.2315234
TAGCTCC250.002364798435.1596942
GGGCTAA451.0473832E-634.17307744
TACGGTG551.3814497E-731.97266828
TTTTCGC353.158529E-431.52418720
TTCGGTA801.3096724E-1030.22416126
CGAGAAC300.00572780129.3481822
TCGGTGG602.9694638E-729.29974442
GAGCATG300.00578794629.2855249
CCACATG300.00578794629.2855249
ACAAATG703.551213E-828.31104524
CGCAAAT406.989934E-427.52462438
CGTTCGG905.0749804E-1026.91296824
TCGTTCG905.0931703E-1026.90249823
CGGTAAG905.16593E-1026.8659228
TCTAACT508.183521E-526.46999421
CGACAAT756.870505E-826.44937317
TCATTGA757.034396E-826.38513825
GGTATGA858.312782E-925.85271341
ACGGTGA701.1376414E-625.12382329
Produced by FastQC (version 0.11.5)