FastQCFastQC Report
Wed 25 May 2016
SRR1780731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515454
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT105122.039367237425648No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT37030.718395821935614RNA PCR Primer, Index 14 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC14250.2764553190003376No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG13780.26733714356664223No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11950.23183446049501993No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC5230.10146395216643969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG250.00234752835.2122714
GCAACGG250.00234752835.2122713
TGCAACG250.00234752835.2122712
TTGCGAT250.002350883635.2020216
ACTTCGC451.0367894E-634.2175458
CGGTAAG451.0367894E-634.21754528
CCGTCTT4200.033.5224844
GCCGTCT4400.031.9987343
GAGGCGC2550.031.93269313
ACGTTGA353.2179008E-431.42427431
GACGTTG353.2179008E-431.42427430
CCGGAAT353.2179008E-431.4242743
GGCTAAA353.2179008E-431.42427428
CGCCATT2600.031.3064517
ATCACTA502.3515822E-630.81073415
TTCGGTA502.3587145E-630.79877726
ATTGGCG2700.030.14987821
TGTCGGG300.005732224329.34355510
TTGTTCG300.005737664629.3378629
AGCCGGG300.005740386429.33501621
Produced by FastQC (version 0.11.5)