FastQCFastQC Report
Wed 25 May 2016
SRR1780730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37390
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCAG150.002139374170.558711
ATTAAGT150.002209048569.9919735
GTCCGGT150.002209048569.9919769
TCCGGTG150.002209048569.9919770
GTGTTAG252.2699177E-456.4469641
CTTTAGG200.006685779452.919031
TTTAGAA200.00690288752.49397743
AAATCTA200.00690288752.49397713
TATTCGC200.00690288752.49397740
CTATTCG200.00690288752.49397739
CCTACTA450.004258205631.107542
GACGAGC952.7143722E-422.10272670
CGACGAG1355.7776633E-620.73836170
CCGACGA1602.5022782E-517.49799269
CTGCCGA2153.1101197E-413.02176170
GCTGCCG2500.001106320911.19871569
CGCTGCC2550.001305564510.97913368
ACGCTGC2700.002102624410.36918167
GACGCTG3258.838744E-49.691195570
TGACGCT3550.00198000938.87222269
Produced by FastQC (version 0.11.5)