FastQCFastQC Report
Wed 25 May 2016
SRR1780730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37390
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT3210.8585183204065258RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2420.6472318801818668No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC640.17116876170098957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCC150.002208043870.024
TGGGCCT150.002208043870.070
TGGAGGG200.00689975752.512
GTTTCGT200.00689975752.524
GATCCCG200.00689975752.518
TTCGTGC200.00689975752.526
CACGACA200.00689975752.519
GGATAAG200.00689975752.51
CCCGTTT200.00689975752.521
GGAAGAG200.00689975752.527
TCCAGGT200.00689975752.52
CTTATGT200.00689975752.54
CTTGAGG200.00689975752.51
GAAAATT200.00689975752.570
ACTGTAA200.00689975752.526
TCCCGTT200.00689975752.520
CGTTTCG200.00689975752.523
ACACTAT400.002389690135.01
CTCGTAT1400.032.50000439
GAAGCGA450.004255815431.11110947
Produced by FastQC (version 0.11.5)