##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 37390 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.169537309441026 34.0 34.0 34.0 27.0 34.0 2 32.98296335918695 34.0 34.0 34.0 32.0 34.0 3 33.27587590264777 34.0 34.0 34.0 32.0 34.0 4 33.181786573950255 34.0 34.0 34.0 32.0 34.0 5 33.211553891414816 34.0 34.0 34.0 32.0 34.0 6 36.84782027280021 38.0 37.0 38.0 35.0 38.0 7 36.499063920834445 38.0 37.0 38.0 34.0 38.0 8 36.35116341267719 38.0 37.0 38.0 34.0 38.0 9 36.248702861727736 38.0 37.0 38.0 34.0 38.0 10-11 35.82075421235624 38.0 36.5 38.0 32.5 38.0 12-13 35.783966301150045 38.0 36.0 38.0 31.0 38.0 14-15 36.03319069269858 38.0 36.5 38.0 32.5 38.0 16-17 36.2213559775341 38.0 37.0 38.0 33.5 38.0 18-19 36.16726397432468 38.0 37.0 38.0 34.0 38.0 20-21 35.90358384594812 38.0 36.5 38.0 31.5 38.0 22-23 35.869550682000536 38.0 36.5 38.0 32.5 38.0 24-25 35.05274137469912 37.0 35.0 38.0 29.0 38.0 26-27 35.39221717036641 37.0 36.0 38.0 31.0 38.0 28-29 35.17820272800214 37.5 35.5 38.0 29.0 38.0 30-31 34.73209414281894 37.0 34.5 38.0 26.5 38.0 32-33 35.39396897566194 37.5 36.0 38.0 31.0 38.0 34-35 35.412556833377906 37.5 36.0 38.0 29.0 38.0 36-37 35.6350895961487 38.0 36.0 38.0 31.0 38.0 38-39 35.281131318534364 37.5 35.5 38.0 29.0 38.0 40-41 34.593968975661944 37.0 34.5 38.0 26.5 38.0 42-43 35.07425782294731 37.0 35.0 38.0 29.0 38.0 44-45 35.335450655255414 37.0 36.0 38.0 29.0 38.0 46-47 35.65445306231612 38.0 36.0 38.0 31.0 38.0 48-49 35.306124632254615 37.5 36.0 38.0 29.0 38.0 50-51 35.60738165284835 38.0 36.0 38.0 31.0 38.0 52-53 35.49927788178657 38.0 36.0 38.0 31.0 38.0 54-55 35.697178389943836 38.0 36.0 38.0 31.5 38.0 56-57 35.798448783097086 38.0 36.5 38.0 31.5 38.0 58-59 35.89772666488366 38.0 37.0 38.0 32.0 38.0 60-61 35.39194971917625 38.0 36.5 38.0 29.5 38.0 62-63 35.68515645894624 38.0 36.0 38.0 31.5 38.0 64-65 35.77161005616475 38.0 36.5 38.0 31.5 38.0 66-67 35.02416421503075 37.5 35.5 38.0 27.0 38.0 68-69 34.33460818400642 37.0 34.0 38.0 24.5 38.0 70-71 34.31366675581707 37.0 34.0 38.0 25.0 38.0 72-73 33.97988767050013 37.0 34.0 38.0 23.5 38.0 74-75 34.7918694838192 37.0 35.0 38.0 27.0 38.0 76 34.221636801283765 37.0 34.0 38.0 25.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7760049343181947 1101 2 0.36231051646444 1101 3 0.13249134701158738 1101 4 0.4497711209263855 1101 5 0.3295309071396417 1101 6 -1.0838330099840192 1101 7 -0.03288587227658013 1101 8 1.1579845989966628 1101 9 0.33047729489462796 1101 10-11 0.1631500194599056 1101 12-13 1.4726205542081203 1101 14-15 1.144732936157844 1101 16-17 0.5661840808969814 1101 18-19 0.06922426585123276 1101 20-21 0.509040805485462 1101 22-23 1.366471871726894 1101 24-25 -2.097275093851266 1101 26-27 0.1446299226219594 1101 28-29 1.5361741285219352 1101 30-31 1.503858415708855 1101 32-33 0.947705570100986 1101 34-35 1.085325340878363 1101 36-37 1.419107338243336 1101 38-39 1.4625671099064164 1101 40-41 1.83567406414479 1101 42-43 1.035148652220613 1101 44-45 1.1713729525441963 1101 46-47 1.3131369452097132 1101 48-49 -1.0034863313679736 1101 50-51 -1.2327736796769457 1101 52-53 -1.7196426313824702 1101 54-55 -0.6322579545236522 1101 56-57 -0.9494745978546746 1101 58-59 -1.0471302100844468 1101 60-61 -1.097921733547203 1101 62-63 -0.12913535404562282 1101 64-65 -2.069954887986718 1101 66-67 -1.5462536575963384 1101 68-69 0.3605545809069355 1101 70-71 -0.18352986148084938 1101 72-73 -1.8049099627844853 1101 74-75 -0.5606678386329023 1101 76 -0.8656019790356453 1102 1 -1.9735123070611138 1102 2 0.153482930257546 1102 3 0.3772499677012462 1102 4 0.6414262933401815 1102 5 0.07635849334653955 1102 6 0.600029058981498 1102 7 0.17056240358549246 1102 8 0.7516397714104599 1102 9 0.7954428121360095 1102 10-11 0.6180120884254237 1102 12-13 0.5384826231736426 1102 14-15 0.11456052236474079 1102 16-17 0.3879771843452602 1102 18-19 -0.11294814794187147 1102 20-21 1.4400408054854594 1102 22-23 0.8907822165544772 1102 24-25 -5.123619921437474 1102 26-27 -0.6210597325504494 1102 28-29 0.43158792162537907 1102 30-31 2.4844791053640307 1102 32-33 0.7581193632044361 1102 34-35 0.9407736167404224 1102 36-37 -2.3619616272738995 1102 38-39 0.382394696113316 1102 40-41 1.1521568227654768 1102 42-43 1.1674589970482003 1102 44-45 1.7272695042683424 1102 46-47 1.9228955658993598 1102 48-49 -1.9283828830921124 1102 50-51 0.08195045825409153 1102 52-53 0.10956426516925433 1102 54-55 1.3073627351315196 1102 56-57 1.3832495400763563 1102 58-59 0.4507663416396923 1102 60-61 -6.214576905960996 1102 62-63 -2.5360663885283827 1102 64-65 0.10207959477190087 1102 66-67 1.7395049630933102 1102 68-69 -1.1196178328861706 1102 70-71 -1.153219516653266 1102 72-73 -5.229840997267246 1102 74-75 -1.8514264593225533 1102 76 -0.014429565242544129 1103 1 1.1438210262722208 1103 2 0.740887185576689 1103 3 0.4184414570629471 1103 4 0.7192135273827347 1103 5 0.7683301245522145 1103 6 0.4473482079176705 1103 7 1.109569495784072 1103 8 0.8405475728288963 1103 9 0.6866768546892033 1103 10-11 0.6757142160849909 1103 12-13 0.026851417499884178 1103 14-15 0.08264562874771997 1103 16-17 0.12891335455802277 1103 18-19 1.2163568165971341 1103 20-21 0.1109911600953879 1103 22-23 0.7289382449232775 1103 24-25 -2.7232227583169077 1103 26-27 0.14716721780415298 1103 28-29 0.48075104219275744 1103 30-31 1.572762793307291 1103 32-33 1.6396229093037178 1103 34-35 0.8306175883716307 1103 36-37 -0.413706308124965 1103 38-39 0.05473512164522987 1103 40-41 0.10242632631157988 1103 42-43 0.3302391388921677 1103 44-45 1.705397163842811 1103 46-47 1.5503281900128414 1103 48-49 -2.0206665710353704 1103 50-51 -0.35924103110761507 1103 52-53 0.7947557545309607 1103 54-55 0.3754762103088254 1103 56-57 1.2378878379486977 1103 58-59 0.35036917851913074 1103 60-61 -3.6625769059609965 1103 62-63 -0.5835557502305093 1103 64-65 0.4435264032825401 1103 66-67 0.0951929063557202 1103 68-69 0.7293183373265961 1103 70-71 0.9502131074602147 1103 72-73 -4.3426636923027075 1103 74-75 -1.5540647571948938 1103 76 -3.199791267370202 1104 1 0.6737970454568725 1104 2 0.4275548727035883 1104 3 0.5918686727372204 1104 4 -0.7436672318396802 1104 5 -0.694346542624686 1104 6 0.32871970646350945 1104 7 0.6281163604200231 1104 8 1.0562728649356359 1104 9 1.5541190711288166 1104 10-11 -0.5277145302796171 1104 12-13 0.8294178749722079 1104 14-15 0.8088051266812855 1104 16-17 0.6166678318272716 1104 18-19 1.2332964563746742 1104 20-21 0.24015591339912845 1104 22-23 0.6873289791444108 1104 24-25 1.4628836756848287 1104 26-27 1.5536165264423545 1104 28-29 1.9073289288196236 1104 30-31 1.4585222708316579 1104 32-33 1.1938603703986743 1104 34-35 0.7067592282512223 1104 36-37 1.7539808187692643 1104 38-39 1.7903083651780634 1104 40-41 1.93670358535541 1104 42-43 0.8560057596381228 1104 44-45 1.2154997200956785 1104 46-47 1.6744926882015179 1104 48-49 0.11155956295105796 1104 50-51 0.9817202424267464 1104 52-53 0.9142261356728625 1104 54-55 0.43121885023943207 1104 56-57 0.871134432162691 1104 58-59 -0.4076797015257725 1104 60-61 0.8446173386433244 1104 62-63 0.9892285755003911 1104 64-65 1.0616191631172214 1104 66-67 0.771936617769569 1104 68-69 1.6739073469699477 1104 70-71 2.3297732891021354 1104 72-73 1.3871949739557792 1104 74-75 -0.42112430104916854 1104 76 -0.7967605004943437 1105 1 1.3712985037496992 1105 2 0.7269423897170029 1105 3 0.662547264998544 1105 4 -0.06327640936252266 1105 5 -0.6094252904372439 1105 6 0.4420290589814968 1105 7 1.0638056468287331 1105 8 0.030072203842891554 1105 9 1.1697130824062825 1105 10-11 1.0504985749119058 1105 12-13 0.2518610015520224 1105 14-15 0.6990199818242004 1105 16-17 0.3887609681290485 1105 18-19 1.2113491493554278 1105 20-21 0.8637705352151883 1105 22-23 0.9890254597977233 1105 24-25 1.4167044028868503 1105 26-27 1.2604267539360308 1105 28-29 1.5666960297334924 1105 30-31 2.174641267526191 1105 32-33 1.7045788226638905 1105 34-35 0.2563411843079919 1105 36-37 1.3070383727260975 1105 38-39 0.7026649663835869 1105 40-41 1.586373038981698 1105 42-43 1.1577022402914423 1105 44-45 1.476242477241307 1105 46-47 1.704679349683147 1105 48-49 0.7761036033943753 1105 50-51 1.5419234312270547 1105 52-53 1.069266967871961 1105 54-55 1.2513627351315222 1105 56-57 1.625898188725003 1105 58-59 1.0888203956937517 1105 60-61 1.492828499444407 1105 62-63 -0.12855287501486856 1105 64-65 1.371214729907038 1105 66-67 1.7338022603906111 1105 68-69 2.0026794644111234 1105 70-71 2.4153750779413343 1105 72-73 1.935537381111132 1105 74-75 0.5815735406774465 1105 76 1.8976785428655631 1106 1 -1.0855123070611157 1106 2 0.45748293025754094 1106 3 0.7372499677012456 1106 4 0.34542629334018216 1106 5 0.14835849334653517 1106 6 0.31202905898149425 1106 7 -0.8054375964145066 1106 8 0.47963977141045433 1106 9 -0.8845571878639902 1106 10-11 0.7500120884254216 1106 12-13 1.770482623173642 1106 14-15 0.5305605223647376 1106 16-17 0.4879771843452545 1106 18-19 1.2190518520581293 1106 20-21 0.8080408054854615 1106 22-23 1.4107822165544803 1106 24-25 -0.03561992143747261 1106 26-27 1.5029402674495458 1106 28-29 0.7915879216253785 1106 30-31 1.21647910536403 1106 32-33 0.8341193632044295 1106 34-35 1.408773616740426 1106 36-37 1.8380383727260963 1106 38-39 0.9903946961133201 1106 40-41 1.8081568227654827 1106 42-43 1.1634589970481954 1106 44-45 1.559269504268336 1106 46-47 1.2148955658993614 1106 48-49 2.1636171169078864 1106 50-51 0.3259504582540842 1106 52-53 0.9495642651692577 1106 54-55 1.059362735131522 1106 56-57 1.0032495400763537 1106 58-59 1.3187663416396944 1106 60-61 1.7374230940390092 1106 62-63 1.59993361147162 1106 64-65 -0.6099204052281024 1106 66-67 0.6195049630933127 1106 68-69 1.6403821671138346 1106 70-71 1.218780483346734 1106 72-73 1.0941590027327521 1106 74-75 -0.1794264593225492 1106 76 1.3775704347574589 1107 1 0.7202099151611066 1107 2 -0.08668373640912108 1107 3 0.21247218992346717 1107 4 0.4935929600068434 1107 5 0.5493584933465385 1107 6 0.6434179478703825 1107 7 1.0844512924743768 1107 8 0.875417549188235 1107 9 -0.5936127434195484 1107 10-11 0.598734310647643 1107 12-13 -0.38693404349302796 1107 14-15 0.05553274458696933 1107 16-17 0.8515049621230375 1107 18-19 0.9661351853914582 1107 20-21 1.0229296943743478 1107 22-23 0.10342110544336691 1107 24-25 0.3007134118958561 1107 26-27 -0.03161528810601055 1107 28-29 0.9585879216253801 1107 30-31 0.9985902164751437 1107 32-33 1.481230474315545 1107 34-35 0.1332180611848699 1107 36-37 1.5500939282816546 1107 38-39 1.102533585002199 1107 40-41 1.366684600543259 1107 42-43 1.421403441492643 1107 44-45 0.9893806153794529 1107 46-47 1.3271455658993645 1107 48-49 1.5118393391301126 1107 50-51 1.1175615693652006 1107 52-53 0.9047587096137022 1107 54-55 1.664557179575965 1107 56-57 1.3209995400763574 1107 58-59 1.274349674973024 1107 60-61 1.6881175384834464 1107 62-63 1.4725169448049442 1107 64-65 1.118024039216344 1107 66-67 -0.24057837024001572 1107 68-69 1.4970488337805037 1107 70-71 0.44925270556895924 1107 72-73 0.7620201138438674 1107 74-75 -0.9080097926558821 1107 76 0.907625990313015 1108 1 -0.9026270611594747 1108 2 0.23059768435590655 1108 3 0.07836472179960907 1108 4 0.7418853097336182 1108 5 0.4243584933465385 1108 6 -0.09485618692013986 1108 7 -0.0032080882177893955 1108 8 -0.38186842531085574 1108 9 -0.10875390917546923 1108 10-11 0.33450389170410944 1108 12-13 1.670613770714624 1108 14-15 0.7702982272827725 1108 16-17 -0.3724818320481802 1108 18-19 0.366920704517149 1108 20-21 1.2446965431903791 1108 22-23 0.4814379542593983 1108 24-25 1.428445652333025 1108 26-27 0.4809074805642979 1108 28-29 -0.6459202750959321 1108 30-31 0.6600200889705903 1108 32-33 1.230775100909348 1108 34-35 0.7092654200191149 1108 36-37 1.7107268973162633 1108 38-39 1.5143619092280645 1108 40-41 1.6242551834212193 1108 42-43 0.014868833113773405 1108 44-45 1.0442531108257143 1108 46-47 1.601223434751823 1108 48-49 1.6420761333013303 1108 50-51 1.5709996385819593 1108 52-53 0.5694331176282788 1108 54-55 0.3825102761151271 1108 56-57 1.276429867945211 1108 58-59 1.0019794563937978 1108 60-61 1.5833247333832645 1108 62-63 1.6038352508158766 1108 64-65 1.272964840673545 1108 66-67 1.7008492253883958 1108 68-69 2.5353001999007176 1108 70-71 -0.13413754944014755 1108 72-73 -0.16620165300495415 1108 74-75 0.2065735406774465 1108 76 1.797767156068936 1109 1 -0.4143185086115011 1109 2 0.5601185891722693 1109 3 0.2683507428950449 1109 4 0.41655807628591646 1109 5 0.08714919102095564 1109 6 -0.14905621233633326 1109 7 -0.630987984011405 1109 8 0.6073296938910744 1109 9 0.9382025020584877 1109 10-11 0.36439968532464917 1109 12-13 0.9054593673596898 1109 14-15 1.281227189031405 1109 16-17 1.0032329982987491 1109 18-19 1.1553309218255734 1109 20-21 1.2134981698265435 1109 22-23 0.6731077979498323 1109 24-25 0.3404420940664039 1109 26-27 0.011343368224743244 1109 28-29 -0.9629237062816003 1109 30-31 0.9504636014880603 1109 32-33 1.4670185880106317 1109 34-35 0.5957503609264734 1109 36-37 1.3448290704005217 1109 38-39 1.1707667891365716 1109 40-41 -0.387595115219014 1109 42-43 1.0627148110016904 1109 44-45 0.952881907369111 1109 46-47 0.9926009922559516 1109 48-49 1.0279892099311496 1109 50-51 -0.16651465802498677 1109 52-53 -0.2545597658384935 1109 54-55 0.08857203745709796 1109 56-57 0.6342262842624038 1109 58-59 0.5735260315621815 1109 60-61 1.1591285203955906 1109 62-63 0.4839026037196774 1109 64-65 1.2147617653145417 1109 66-67 0.9084041878995137 1109 68-69 0.27030464773398677 1109 70-71 0.3265944368351086 1109 72-73 -0.5620270437788761 1109 74-75 0.01471307556116841 1109 76 1.5143766363078441 1110 1 -0.3166309511289107 1110 2 -0.9570933409288997 1110 3 -0.3889873204343459 1110 4 -0.2461669269988036 1110 5 0.5684262899567045 1110 6 0.386944313218784 1110 7 0.5959861323990481 1110 8 1.3306906188680827 1110 9 0.9418156934919395 1110 10-11 -0.6690387590322047 1110 12-13 0.8417029621566883 1110 14-15 0.9874418782969485 1110 16-17 0.6537059979045807 1110 18-19 -0.34654136828085313 1110 20-21 1.0747187715871576 1110 22-23 -0.6292177834455188 1110 24-25 -0.6788063621154379 1110 26-27 1.3608046742292146 1110 28-29 1.1301980911169096 1110 30-31 -0.25023275904275266 1110 32-33 0.8277464818485001 1110 34-35 0.6548753116556796 1110 36-37 1.3176315930650802 1110 38-39 1.5086658825539914 1110 40-41 -1.638317753505703 1110 42-43 -1.036812189392478 1110 44-45 1.024455944946304 1110 46-47 0.6878447184417382 1110 48-49 1.4675832186028046 1110 50-51 -0.2382529315764188 1110 52-53 0.5678354516099375 1110 54-55 -0.31219658690237395 1110 56-57 0.9772495400763574 1110 58-59 -1.049979421072166 1110 60-61 0.4942027550559587 1110 62-63 -0.06155791395210741 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2116 58-59 -0.9007175293280483 2116 60-61 -2.017415615638413 2116 62-63 0.3821271598587117 2116 64-65 -2.4010171794216504 2116 66-67 -1.8013982627131355 2116 68-69 -2.3873597683700396 2116 70-71 0.33690951560480187 2116 72-73 0.2386106156359844 2116 74-75 -0.2611683948064254 2116 76 -1.2749456942747983 2117 1 1.2998493950665448 2117 2 -0.003793665487137332 2117 3 0.16312230812678052 2117 4 -0.1956800896385431 2117 5 0.15840104653802456 2117 6 -0.10584328144403798 2117 7 0.4641794248620883 2117 8 0.06040572885726192 2117 9 -1.7601316559490954 2117 10-11 -0.8987538690213839 2117 12-13 -2.831304610868912 2117 14-15 -1.034375647848023 2117 16-17 -2.637044092250491 2117 18-19 -1.8864800628354814 2117 20-21 0.13935995442162863 2117 22-23 -0.28879225153063004 2117 24-25 -0.886343325692792 2117 26-27 -2.4946767538270507 2117 28-29 -2.9483269719916407 2117 30-31 -0.9875208946359706 2117 32-33 -2.5979657431785483 2117 34-35 0.07175234014468401 2117 36-37 -1.4243446059973053 2117 38-39 -0.09420104856754108 2117 40-41 0.003135546169730219 2117 42-43 -0.04564738593052198 2117 44-45 -1.0818368787103836 2117 46-47 -1.2120831575048854 2117 48-49 -1.2901701171346645 2117 50-51 -0.2954112438735734 2117 52-53 0.6032663928288287 2117 54-55 -1.9401266265706028 2117 56-57 -0.9429632258810869 2117 58-59 -2.103531530700728 2117 60-61 -1.6306620123439757 2117 62-63 -2.030364260868815 2117 64-65 -1.6947714690578906 2117 66-67 -1.0821120581832844 2117 68-69 -1.1111071945882927 2117 70-71 -2.376028027291561 2117 72-73 -1.0412452525863998 2117 74-75 0.2012543917412799 2117 76 -1.2636210546042435 2118 1 -1.7576941252429314 2118 2 0.40384656662117635 2118 3 -0.20293185048057438 2118 4 0.8439717478856323 2118 5 0.24254031152835864 2118 6 0.5556654226178637 2118 7 0.6160169490400378 2118 8 0.1203670441377298 2118 9 -2.0096480969548978 2118 10-11 -0.8791697297563985 2118 12-13 -2.0583355586445364 2118 14-15 0.13728779509201416 2118 16-17 -3.9745682702001943 2118 18-19 -1.0177663297600503 2118 20-21 -1.047777376332725 2118 22-23 0.24578221655448118 2118 24-25 0.433289169471621 2118 26-27 -3.7828779143686333 2118 28-29 -2.370957532920073 2118 30-31 -1.2261572582723304 2118 32-33 -1.9026079095228425 2118 34-35 -1.8566809287141197 2118 36-37 -3.4088707181829925 2118 38-39 -1.1130598493412336 2118 40-41 -0.038934086325426165 2118 42-43 0.9940044515936535 2118 44-45 -2.599003223004388 2118 46-47 -0.6018317068279018 2118 48-49 0.22143529872607104 2118 50-51 -0.510594996291367 2118 52-53 -2.0766175530125537 2118 54-55 -3.191819083050291 2118 56-57 -1.0227504599236426 2118 58-59 -1.6470518401784915 2118 60-61 -3.12848599687009 2118 62-63 -2.437520933982931 2118 64-65 -0.0821022234099118 2118 66-67 -1.9725859459975936 2118 68-69 -2.305981469249801 2118 70-71 -1.4519467893805356 2118 72-73 1.9662499118236596 2118 74-75 -0.11160827750437363 2118 76 0.19866134384836442 2119 1 0.0736455876757276 2119 2 -0.0351486486898267 2119 3 -1.1084342428250693 2119 4 -1.1368894961335059 2119 5 -0.7072204540218792 2119 6 -1.0974446252290306 2119 7 -0.8504902279934541 2119 8 -1.1786760180632285 2119 9 -0.23213613523240895 2119 10-11 -1.5807247536798386 2119 12-13 -0.31730685051056895 2119 14-15 -0.9512289513194716 2119 16-17 -0.6384438682863163 2119 18-19 -1.4346323584681855 2119 20-21 -4.3528012997776955 2119 22-23 -2.0897440992349914 2119 24-25 0.17132744698358238 2119 26-27 -1.561586048339926 2119 28-29 -2.2585173415325173 2119 30-31 -1.4875208946359706 2119 32-33 -1.3667227420587267 2119 34-35 -0.7059632253648331 2119 36-37 -2.3442774167475875 2119 38-39 -6.2518158302024744 2119 40-41 -4.68247475618189 2119 42-43 -1.3421199503202246 2119 44-45 -0.9967304957316614 2119 46-47 -1.118578118311163 2119 48-49 -2.6445934094079036 2119 50-51 -0.9639442785880235 2119 52-53 -1.2847515243044256 2119 54-55 -1.446005685921108 2119 56-57 -4.56222414413417 2119 58-59 -1.7941810267813594 2119 60-61 -0.7152084849083664 2119 62-63 -1.2210137569494393 2119 64-65 -0.8033940894386262 2119 66-67 -0.09638977374878976 2119 68-69 -0.051196780254585406 2119 70-71 -2.679219516653262 2119 72-73 -0.3309988920040894 2119 74-75 -2.0434264593225535 2119 76 -1.9209558810320146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 5.0 23 14.0 24 34.0 25 49.0 26 94.0 27 159.0 28 334.0 29 522.0 30 892.0 31 1359.0 32 2080.0 33 2988.0 34 4511.0 35 6232.0 36 9129.0 37 8986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.900240706071145 23.664081305161808 11.524471783899438 27.91120620486761 2 18.879379513238835 19.614870286172774 36.75849157528751 24.747258625300883 3 18.08504947847018 25.42123562449853 29.026477667825624 27.467237229205672 4 13.487563519657664 18.678791120620485 37.991441561914954 29.8422037978069 5 12.93928857983418 37.70526878844611 35.85183204065258 13.503610591067131 6 29.079967905857178 38.37122225193902 17.2211821342605 15.3276277079433 7 28.49424979941161 30.085584380850495 21.83738967638406 19.58277614335384 8 23.787108852634393 35.675314255148436 20.818400641882857 19.719176250334314 9 23.47686547205135 16.333244182936614 22.324150842471248 37.865739502540784 10-11 24.428991709013108 26.10189890345012 27.61032361594009 21.858785771596683 12-13 24.34875635196577 23.526343942230543 26.817330837122228 25.307568868681468 14-15 21.770526878844613 25.62717304092003 23.617277346884194 28.985022733351162 16-17 22.066060443968976 28.634661674244448 26.297138272265315 23.00213960952126 18-19 21.81064455736828 27.932602300080234 27.078095747526078 23.178657395025407 20-21 21.097887135597755 27.822947312115538 26.79994650976197 24.279219042524737 22-23 22.302754747258625 28.22011232949987 27.404386199518587 22.072746723722922 24-25 22.250601765177855 28.994383525006683 26.765177855041454 21.989836854774005 26-27 20.88258892752073 29.481144691093874 27.508692163680127 22.127574217705266 28-29 20.933404653650708 28.570473388606583 28.22412409735223 22.27199786039048 30-31 20.87991441561915 27.423107782829632 28.21877507354908 23.478202728002138 32-33 21.243648034233754 28.46616742444504 26.888205402514043 23.401979138807167 34-35 21.422840331639474 27.71997860390479 28.824552019256483 22.03262904519925 36-37 22.45386466969778 27.473923508959615 27.785504145493444 22.286707675849158 38-39 21.29713827226531 27.971382722653114 27.801551216902915 22.92992778817866 40-41 21.135330302219845 27.27467237229206 28.931532495319605 22.658464830168494 42-43 21.76116608718909 26.961754479807436 29.035838459481145 22.241240973522334 44-45 20.64589462423108 26.943032896496387 28.864669697780155 23.54640278149238 46-47 21.631452259962558 25.964161540518855 29.086654185611128 23.31773201390746 48-49 20.91735758224124 26.170098956940357 29.84354105375769 23.06900240706071 50-51 19.903717571543194 26.707675849157532 29.835517518052956 23.55308906124632 52-53 19.798074351430863 26.370687349558708 30.899973254880987 22.931265044129447 54-55 18.956940358384593 26.8079700454667 30.20861192832308 24.02647766782562 56-57 19.847552821610055 26.38138539716502 29.31666220914683 24.454399572078096 58-59 19.00240706071142 27.798876705001334 29.40893286975127 23.78978336453597 60-61 19.181599358117143 28.539716501738432 28.653383257555497 23.625300882588927 62-63 18.220112329499866 29.296603369884995 27.86306499063921 24.62021930997593 64-65 19.34340732816261 29.826156726397436 26.317197111527147 24.513238833912812 66-67 18.986359989301953 29.13479539983953 26.412142284033163 25.466702326825352 68-69 18.579834180262104 29.376838726932338 26.325220647231877 25.718106445573685 70-71 18.96228938218775 28.844610858518323 26.49371489703129 25.699384862262637 72-73 19.156191495052152 28.35918694838192 25.8130516180797 26.67156993848623 74-75 18.932869751270392 28.930195239368818 25.391815993581172 26.745119015779622 76 19.085316929660337 29.039850227333513 25.220647231880182 26.654185611125968 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 3.5 17 5.0 18 5.5 19 5.5 20 8.0 21 11.0 22 8.0 23 20.0 24 42.0 25 49.0 26 85.0 27 131.5 28 165.0 29 188.0 30 247.5 31 365.5 32 517.5 33 611.0 34 687.0 35 878.0 36 1102.0 37 1211.0 38 1353.0 39 1755.0 40 2219.5 41 2618.5 42 2813.0 43 2835.5 44 2982.0 45 3080.0 46 3054.0 47 2945.5 48 2695.0 49 2459.5 50 2366.0 51 2134.0 52 1659.0 53 1318.0 54 1220.0 55 1092.5 56 817.5 57 542.0 58 414.0 59 306.5 60 160.5 61 87.5 62 53.0 63 35.5 64 12.0 65 4.5 66 2.0 67 1.0 68 0.5 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 37390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.656860123027556 #Duplication Level Percentage of deduplicated Percentage of total 1 70.75151057401813 40.0855843808505 2 14.04833836858006 15.91869483819203 3 6.117824773413897 10.398502273335117 4 3.243013595166163 7.349558705536239 5 1.8362915407854987 5.201925648569136 6 1.2556646525679758 4.268520994918427 7 0.8827416918429002 3.500936079165552 8 0.547583081570997 2.481947044664349 9 0.33987915407854985 1.7330837122225193 >10 0.9629909365558912 7.384327360256752 >50 0.004720543806646525 0.17116876170098957 >100 0.00944108761329305 1.5057502005883925 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 321 0.8585183204065258 RNA PCR Primer, Index 42 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 242 0.6472318801818668 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 64 0.17116876170098957 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.008023535704733886 0.0 2 0.0 0.0 0.0 0.010698047606311848 0.0 3 0.0 0.0 0.0 0.010698047606311848 0.0 4 0.0 0.0 0.0 0.010698047606311848 0.0 5 0.0 0.0 0.0 0.013372559507889811 0.0 6 0.0 0.0 0.0 0.013372559507889811 0.0 7 0.0 0.0 0.0 0.013372559507889811 0.0 8 0.0 0.0 0.0 0.013372559507889811 0.0 9 0.0 0.0 0.0 0.018721583311045734 0.0 10 0.0 0.0 0.0 0.026745119015779622 0.0 11 0.0 0.0 0.0 0.02941963091735758 0.0 12 0.0 0.0 0.0 0.02941963091735758 0.0 13 0.0 0.0 0.0 0.02941963091735758 0.0 14 0.0 0.0 0.0 0.02941963091735758 0.0 15 0.0 0.0 0.0 0.02941963091735758 0.0 16 0.0 0.0 0.0 0.034768654720513506 0.0 17 0.0 0.0 0.0 0.034768654720513506 0.0 18 0.0 0.0 0.0 0.03744316662209147 0.0 19 0.0 0.0 0.0 0.045466702326825356 0.0 20 0.0 0.0 0.0 0.053490238031559244 0.0 21 0.0 0.0 0.0 0.053490238031559244 0.0 22 0.0 0.0 0.0 0.061513773736293124 0.0 23 0.0 0.0 0.0 0.061513773736293124 0.0 24 0.0 0.0 0.0 0.061513773736293124 0.0 25 0.0 0.0 0.0 0.061513773736293124 0.0 26 0.0 0.0 0.0 0.06953730944102701 0.0 27 0.0 0.0 0.0 0.07488633324418294 0.0 28 0.0 0.0 0.0 0.09360791655522867 0.0 29 0.0 0.0 0.0 0.1123294998662744 0.0 30 0.0 0.0 0.0 0.1257020593741642 0.0 31 0.0 0.0 0.0 0.15244717838994384 0.0 32 0.0 0.0 0.0 0.17919229740572346 0.0 33 0.0 0.0 0.0 0.1898903450120353 0.0 34 0.0 0.0 0.0 0.20593741642150307 0.0 35 0.0 0.0 0.0 0.2460550949451725 0.0 36 0.0 0.0 0.0 0.36373361861460285 0.0 37 0.0 0.0 0.0 0.5215298208077026 0.0 38 0.0 0.0 0.0 0.8210751537844343 0.0 39 0.0 0.0 0.0 1.1553891414816795 0.0 40 0.0 0.0 0.0 1.5164482481947044 0.0 41 0.0 0.0 0.0 2.0460016047071408 0.0 42 0.0 0.0 0.0 2.888472853704199 0.0 43 0.0 0.0 0.0 3.66140679326023 0.0 44 0.0 0.0 0.0 4.3514308638673445 0.0 45 0.0 0.0 0.0 5.004011767852367 0.0 46 0.0 0.0 0.0 5.65659267183739 0.0 47 0.0 0.0 0.0 6.295801016314522 0.0 48 0.0 0.0 0.0 6.951056432201123 0.0 49 0.0 0.0 0.0 7.574217705268788 0.0 50 0.0 0.0 0.0 8.079700454667023 0.0 51 0.0 0.0 0.0 8.697512703931533 0.0 52 0.0 0.0 0.0 9.497191762503343 0.0 53 0.0 0.0 0.0 10.411874832843006 0.0 54 0.0 0.0 0.0 11.224926450922707 0.0 55 0.0 0.0 0.0 12.02193099759294 0.0 56 0.0 0.0 0.0 12.602300080235358 0.0 57 0.0 0.0 0.0 13.19069269858251 0.0 58 0.0 0.0 0.0 13.755014709815459 0.0 59 0.0 0.0 0.0 14.399572078095748 0.0 60 0.0 0.0 0.0 14.886333244182937 0.0 61 0.0 0.0 0.0 15.52286707675849 0.0 62 0.0 0.0 0.0 16.146028349826157 0.0 63 0.0 0.0 0.0 16.779887670500134 0.0 64 0.0 0.0 0.0 17.526076491040385 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCACCC 15 0.0022080438 70.0 24 TGGGCCT 15 0.0022080438 70.0 70 TGGAGGG 20 0.006899757 52.5 12 GTTTCGT 20 0.006899757 52.5 24 GATCCCG 20 0.006899757 52.5 18 TTCGTGC 20 0.006899757 52.5 26 CACGACA 20 0.006899757 52.5 19 GGATAAG 20 0.006899757 52.5 1 CCCGTTT 20 0.006899757 52.5 21 GGAAGAG 20 0.006899757 52.5 27 TCCAGGT 20 0.006899757 52.5 2 CTTATGT 20 0.006899757 52.5 4 CTTGAGG 20 0.006899757 52.5 1 GAAAATT 20 0.006899757 52.5 70 ACTGTAA 20 0.006899757 52.5 26 TCCCGTT 20 0.006899757 52.5 20 CGTTTCG 20 0.006899757 52.5 23 ACACTAT 40 0.0023896901 35.0 1 CTCGTAT 140 0.0 32.500004 39 GAAGCGA 45 0.0042558154 31.111109 47 >>END_MODULE