FastQCFastQC Report
Wed 25 May 2016
SRR1780729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794776
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCG207.8401173E-444.0169531
ATTCACC505.941365E-835.20025322
TGACTTA250.002352224735.20025322
TTGCGAT803.6379788E-1233.05646516
CCCGTGC1850.030.8763478
CGTGCGA1800.030.5827510
CGAGAAT1800.029.38167214
CCGGTTT300.005701318429.37796613
CGCACTT300.005768439329.30769527
AACGTTT300.00577731729.29847344
GTGACAT300.00577909429.296632
CTAGAGA300.005782649429.2929463
TGAACGA407.0296944E-427.50019822
GGTCGCG407.08684E-427.4621357
GGTACAA1006.184564E-1126.37692630
CCGTGCG2000.026.363659
ATGAACG450.001382919324.4893621
GAATCAG450.001392609924.4600336
GCTTTAC450.001394657124.4538631
CATCGTT1102.0372681E-1023.97449532
Produced by FastQC (version 0.11.5)