FastQCFastQC Report
Wed 25 May 2016
SRR1780729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794776
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT51280.645213242473351No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT21340.26850332672350447TruSeq Adapter, Index 3 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC9640.12129203700162057No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC8800.1107230213292802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA207.858721E-443.99578536
TTCGCTA207.858721E-443.99578523
GGTGAAT301.2990602E-436.6746914
TCCGTCG301.3014674E-436.6631528
GTATTAA250.002342539435.2298741
GGTCTAA250.002342539435.2298741
TGACCGG250.002353415635.1966331
CCGTCTT3750.034.61219844
GCCGTCT3650.033.14959343
TTGCGAT401.8181354E-533.00306716
CGTGCGA1300.032.1608810
CATCGTT900.031.77473432
CTCAATG353.217499E-431.42753641
CGAGAAT1350.030.9697414
CCGTGCG1350.030.9638949
CCAACGT801.3096724E-1030.247122
TCGTTGT951.8189894E-1230.10237934
GCACTAC300.005720079429.3582271
TGCAAGT300.005737705629.3397514
ATGCAGT300.005744768329.3323676
Produced by FastQC (version 0.11.5)