##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780728_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13403 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.216071028874133 34.0 27.0 34.0 12.0 34.0 2 30.659180780422293 34.0 32.0 34.0 21.0 34.0 3 30.817354323658883 34.0 32.0 34.0 21.0 34.0 4 31.05192867268522 34.0 33.0 34.0 21.0 34.0 5 30.9227038722674 34.0 34.0 34.0 21.0 34.0 6 33.32955308513019 38.0 34.0 38.0 11.0 38.0 7 33.397075281653365 38.0 34.0 38.0 21.0 38.0 8 32.271655599492654 37.0 31.0 38.0 11.0 38.0 9 33.228978586883535 37.0 34.0 38.0 21.0 38.0 10-11 33.25042900843095 37.5 34.0 38.0 16.0 38.0 12-13 33.398269044243825 38.0 34.0 38.0 16.0 38.0 14-15 33.225322688950236 38.0 34.0 38.0 11.0 38.0 16-17 33.030664776542565 38.0 34.0 38.0 11.5 38.0 18-19 33.062672535999404 37.5 34.0 38.0 11.0 38.0 20-21 33.1409759009177 38.0 34.0 38.0 16.0 38.0 22-23 33.019100201447436 37.5 34.0 38.0 11.0 38.0 24-25 33.429120346191155 38.0 34.0 38.0 16.0 38.0 26-27 33.32522569573976 38.0 34.0 38.0 11.5 38.0 28-29 33.44807132731478 38.0 34.0 38.0 11.0 38.0 30-31 33.32328583153026 38.0 34.0 38.0 11.0 38.0 32-33 32.97780347683354 38.0 34.0 38.0 11.0 38.0 34-35 33.15627098410803 37.0 34.0 38.0 11.0 38.0 36-37 33.06412743415653 37.5 34.0 38.0 11.0 38.0 38-39 33.434604193091104 38.0 34.0 38.0 11.0 38.0 40-41 33.090763261956276 37.5 34.0 38.0 11.0 38.0 42-43 33.26613444751175 38.0 34.0 38.0 11.0 38.0 44-45 32.461389241214654 37.0 31.5 38.0 11.0 38.0 46-47 33.1196746996941 37.0 34.0 38.0 11.0 38.0 48-49 32.9622099529956 37.0 34.0 38.0 11.0 38.0 50-51 32.77609490412594 37.0 33.0 38.0 11.0 38.0 52-53 32.802432291278066 37.0 33.0 38.0 11.0 38.0 54-55 32.6979780646124 37.0 32.0 38.0 11.0 38.0 56-57 32.67518466015071 37.0 32.0 38.0 11.0 38.0 58-59 32.623666343355964 37.0 32.0 38.0 11.0 38.0 60-61 32.7072297246885 37.0 32.5 38.0 11.0 38.0 62-63 32.60557337909424 37.0 32.0 38.0 11.0 38.0 64-65 32.69961948817429 37.0 32.0 38.0 11.0 38.0 66-67 32.61325822577035 37.0 31.5 38.0 11.0 38.0 68-69 32.18790569275535 37.0 30.5 38.0 11.0 38.0 70-71 32.27329702305454 37.0 31.0 38.0 11.0 38.0 72-73 32.40293217936283 37.0 31.0 38.0 11.0 38.0 74-75 31.90058195926285 37.0 29.5 38.0 11.0 38.0 76 31.326270238006416 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.9447402249642529 1101 2 1.3843501573323884 1101 3 1.7692149508070543 1101 4 -1.381528746873144 1101 5 0.01265721792031016 1101 6 -0.9684501216773924 1101 7 -1.6412804842594504 1101 8 1.2627664467385955 1101 9 1.386419112972348 1101 10-11 0.8309834193325543 1101 12-13 0.05232841317896941 1101 14-15 0.9804336461106473 1101 16-17 -0.7065465987042572 1101 18-19 0.15213668961276028 1101 20-21 0.131597101592277 1101 22-23 0.31743310500790045 1101 24-25 -1.0622525355914902 1101 26-27 -0.2079123272063086 1101 28-29 0.7979126361646749 1101 30-31 -0.2568775228301874 1101 32-33 0.7652054156281665 1101 34-35 0.08448351111135821 1101 36-37 1.4662010892506885 1101 38-39 -0.35706348215753536 1101 40-41 0.7997907786077718 1101 42-43 1.0617839733647259 1101 44-45 0.9281931620775552 1101 46-47 1.2674961605391246 1101 48-49 0.8235556715487959 1101 50-51 0.299317783472695 1101 52-53 0.7598665383299945 1101 54-55 0.10084575647122307 1101 56-57 0.0032419936833605334 1101 58-59 -0.5950856877437829 1101 60-61 0.645492197443204 1101 62-63 -0.2212148480035765 1101 64-65 1.372956288260447 1101 66-67 0.27186689027709576 1101 68-69 1.7409233600070806 1101 70-71 1.4627399996988402 1101 72-73 1.0821122259779727 1101 74-75 -0.021294977239328716 1101 76 1.0504131135307162 1102 1 1.6352993007274463 1102 2 1.4545082600991925 1102 3 -0.25845303338266845 1102 4 1.273609593047805 1102 5 0.6124595894618139 1102 6 1.798348297294936 1102 7 1.7124744564520071 1102 8 2.607628106817643 1102 9 0.6571701011146374 1102 10-11 1.2173470556961945 1102 12-13 -2.7633830492716243 1102 14-15 -0.7527679349170171 1102 16-17 -1.282139484079753 1102 18-19 1.3260497330910255 1102 20-21 1.3465180502088714 1102 22-23 0.4799825121225254 1102 24-25 1.7099806659895407 1102 26-27 -3.553762129577855 1102 28-29 0.9634264701567687 1102 30-31 1.3211857182765314 1102 32-33 -6.463549525083298 1102 34-35 -0.19022004620089206 1102 36-37 0.5220311287763764 1102 38-39 0.4018297984748713 1102 40-41 0.6234073793982802 1102 42-43 0.41356262949121003 1102 44-45 -5.620719881400703 1102 46-47 0.3475356862308274 1102 48-49 0.4599193079124362 1102 50-51 -6.331116999136 1102 52-53 -0.42392792807316937 1102 54-55 1.7974860726767616 1102 56-57 -0.09656037785814675 1102 58-59 1.7230961304380301 1102 60-61 1.8678242132534706 1102 62-63 -4.733566626659702 1102 64-65 0.23856893648179067 1102 66-67 0.6325388270359866 1102 68-69 -7.217080592562095 1102 70-71 -1.0016868777715118 1102 72-73 0.13102526945623794 1102 74-75 -6.53611711162668 1102 76 -1.686741036666909 1103 1 -0.5263645071627749 1103 2 0.6427586889156558 1103 3 0.32130682939606103 1103 4 -0.247402413813262 1103 5 -0.13582514467197981 1103 6 -2.904053074917755 1103 7 0.9534693106544125 1103 8 0.13807407594286047 1103 9 -0.5040648903090315 1103 10-11 0.2083419098985928 1103 12-13 -0.836925073285343 1103 14-15 -0.25233911845046464 1103 16-17 -0.6048238751603705 1103 18-19 -0.7399880027580252 1103 20-21 1.040128684857251 1103 22-23 -0.980351964721379 1103 24-25 0.8165415579277848 1103 26-27 -2.113367618428626 1103 28-29 0.2886837600367045 1103 30-31 -0.24313675170631655 1103 32-33 -4.405016077398905 1103 34-35 -0.6880759638681369 1103 36-37 0.19077040836471326 1103 38-39 0.7916239665709313 1103 40-41 0.8547538631032623 1103 42-43 0.6905780668840009 1103 44-45 -3.8777953530988185 1103 46-47 -0.8141281216593939 1103 48-49 0.1490273696620008 1103 50-51 0.41888300086399965 1103 52-53 -0.135334446083462 1103 54-55 -0.22052421891843466 1103 56-57 0.6281394506152722 1103 58-59 0.8388765764071664 1103 60-61 1.9192821892397518 1103 62-63 0.475052584318 1103 64-65 0.1832516122965444 1103 66-67 0.2663295646003121 1103 68-69 -2.154687796678733 1103 70-71 0.5999426248013862 1103 72-73 2.001308288324161 1103 74-75 -0.9593589641138109 1103 76 -0.08125218589503902 1104 1 -1.065609790181643 1104 2 -1.079128103537176 1104 3 -0.6820893970190305 1104 4 -0.5145722251340139 1104 5 -1.0229949559927327 1104 6 -2.798015339068698 1104 7 -0.24388917991162629 1104 8 0.695809924999466 1104 9 -1.5101026261580905 1104 10-11 -2.2990165806674447 1104 12-13 -2.7720194129079836 1104 14-15 -1.9191315712806585 1104 16-17 -2.103503120443392 1104 18-19 -0.9939502669089748 1104 20-21 -1.4666637679729426 1104 22-23 -1.2586538515138344 1104 24-25 -1.5113829703741004 1104 26-27 -1.2792166750324014 1104 28-29 -1.553391711661412 1104 30-31 -2.3442688271780128 1104 32-33 -2.2721858887196618 1104 34-35 -1.872038228019079 1104 36-37 -2.60205978031453 1104 38-39 -1.6927156560705825 1104 40-41 -0.9897744387835345 1104 42-43 -0.26734646141788687 1104 44-45 -4.7957198814007 1104 46-47 -1.733373404678268 1104 48-49 -1.396444328451203 1104 50-51 -1.2011169991359978 1104 52-53 -1.5866552008004433 1104 54-55 -1.9952412000505113 1104 56-57 -1.4511058324035986 1104 58-59 -0.3650856877437789 1104 60-61 -0.4258121503828889 1104 62-63 -2.249475717568796 1104 64-65 -1.4205219726091158 1104 66-67 -1.2129157184185608 1104 68-69 -2.8629896834711843 1104 70-71 -1.0898686959533315 1104 72-73 -0.1939747305437649 1104 74-75 -1.235208020717586 1104 76 -1.6121955821214549 1105 1 -1.3624725352796823 1105 2 -0.012069280007761307 1105 3 0.6132047206280262 1105 4 0.0485650297679463 1105 5 -0.8594655442280263 1105 6 0.2698277981862063 1105 7 0.2623853298922967 1105 8 -0.6281116436279888 1105 9 0.5181326679595557 1105 10-11 -0.36509501203999406 1105 12-13 -1.8206468638883777 1105 14-15 -0.5116805908885027 1105 16-17 0.13786942857621654 1105 18-19 -0.2915973257325035 1105 20-21 -1.4937225915023546 1105 22-23 0.10899320730969464 1105 24-25 -0.5415790488054739 1105 26-27 0.43509705045779157 1105 28-29 -0.4806466136221985 1105 30-31 -0.763484513452525 1105 32-33 -0.3933623593078934 1105 34-35 -0.6808617574308471 1105 36-37 -0.9385303685498236 1105 38-39 -0.26349996979607226 1105 40-41 -0.4227156152541198 1105 42-43 0.30304569544486526 1105 44-45 1.1660448244816521 1105 46-47 0.20270502669428936 1105 48-49 -0.6421306029610108 1105 50-51 -0.6595483716850197 1105 52-53 -0.6427336321729911 1105 54-55 -1.553672572599531 1105 56-57 -1.4530666167173258 1105 58-59 -0.7519484328418216 1105 60-61 -0.9958121503828892 1105 62-63 -1.2632012077648724 1105 64-65 -1.7636592275110772 1105 66-67 -0.5991902282224828 1105 68-69 -0.6633818403339298 1105 70-71 0.07719012757608112 1105 72-73 -0.5577002207398394 1105 74-75 -1.5873648834626835 1105 76 -0.17337205270969136 1106 1 0.3060181167951015 1106 2 0.31761608250933904 1106 3 -0.7616242807399622 1106 4 0.5310091702148227 1106 5 0.3402608579607538 1106 6 0.7317521027917664 1106 7 1.715645703809301 1106 8 3.454879692441324 1106 9 1.4647810947721425 1106 10-11 0.6798206286348787 1106 12-13 0.24798058709201598 1106 14-15 0.38412424267283285 1106 16-17 0.880915484207776 1106 18-19 -0.9762758483043257 1106 20-21 -2.195500977275266 1106 22-23 -0.7760957119789467 1106 24-25 0.8207100528817151 1106 26-27 1.406597278455969 1106 28-29 -0.8899033395683915 1106 30-31 -1.4335711527594093 1106 32-33 -0.8940463538359396 1106 34-35 0.8314501440739406 1106 36-37 0.540265801080821 1106 38-39 -0.36085519095430385 1106 40-41 -0.6860535085509767 1106 42-43 0.3459093525355996 1106 44-45 0.870559188366741 1106 46-47 0.9252312464845218 1106 48-49 -0.565979212172131 1106 50-51 -0.5694890921592588 1106 52-53 -1.0861900845213697 1106 54-55 0.08289833483321019 1106 56-57 0.43726626061965845 1106 58-59 -1.3741554551856439 1106 60-61 0.9693041286868791 1106 62-63 0.22494288708237065 1106 64-65 0.49808267855367205 1106 66-67 0.5056889327442349 1106 68-69 0.20073124676137155 1106 70-71 0.1394336296280585 1106 72-73 -0.11606775379957668 1106 74-75 -2.9742777881594478 1106 76 -1.3484746518888961 1107 1 0.5177944651375057 1107 2 1.2983187049734646 1107 3 1.526846773193732 1107 4 1.035215008908537 1107 5 1.7425369589008852 1107 6 1.7896442353993862 1107 7 1.2995150754075198 1107 8 2.260916307978185 1107 9 1.1852165227780844 1107 10-11 0.2352387384814847 1107 12-13 1.1309593104962659 1107 14-15 0.6800173648895509 1107 16-17 1.504369219982138 1107 18-19 1.9430710096867756 1107 20-21 1.7703575086228085 1107 22-23 0.8743248718904155 1107 24-25 1.9805319232429213 1107 26-27 1.71461311220164 1107 28-29 0.8268210542960333 1107 30-31 1.828922662183686 1107 32-33 1.7818566644718246 1107 34-35 1.069451133683046 1107 36-37 1.8017700069195115 1107 38-39 1.6121779609506959 1107 40-41 1.004693646322849 1107 42-43 1.2694620492204152 1107 44-45 0.5627907568971722 1107 46-47 1.6023712761727964 1107 48-49 0.6480237566551779 1107 50-51 1.9933510859703816 1107 52-53 0.7767490545187101 1107 54-55 1.1417800765452384 1107 56-57 2.0446388484474625 1107 58-59 1.9687440994902587 1107 60-61 0.9935495517447706 1107 62-63 1.5824391760482257 1107 64-65 1.5475631337738633 1107 66-67 2.20410555817719 1107 68-69 1.8170103165288154 1107 70-71 1.9833227934083766 1107 72-73 1.873046546051981 1107 74-75 2.67713240481433 1107 76 1.3635490987296066 1108 1 1.166483233074171 1108 2 1.526918408090733 1108 3 2.2383757192600413 1108 4 0.8333347516101703 1108 5 2.17747016028634 1108 6 2.0108218702336274 1108 7 1.8784364014837251 1108 8 1.059530855232019 1108 9 -1.7212654168557648 1108 10-11 0.8077276053790641 1108 12-13 1.9805387266269037 1108 14-15 2.372496335696084 1108 16-17 -0.7004798646294361 1108 18-19 0.337677640067767 1108 20-21 1.5951966971433365 1108 22-23 0.5727414973233707 1108 24-25 0.13466354125380775 1108 26-27 0.22055076682806174 1108 28-29 1.2845152650827742 1108 30-31 2.7175916379382627 1108 32-33 1.7106048089547627 1108 34-35 0.738426888259994 1108 36-37 1.1100332429412845 1108 38-39 1.813563413696862 1108 40-41 1.8720860263327452 1108 42-43 2.694746561837931 1108 44-45 2.2659080255760387 1108 46-47 2.9601149674147536 1108 48-49 1.7479742761999617 1108 50-51 1.5700457915616681 1108 52-53 0.727763403850723 1108 54-55 1.9898750790192565 1108 56-57 2.088429051317327 1108 58-59 -0.21136475751122674 1108 60-61 2.7600018031054816 1108 62-63 1.5272684684777147 1108 64-65 1.777152445995533 1108 66-67 0.7963866071628303 1108 68-69 2.607707990947418 1108 70-71 3.5580382807908535 1108 72-73 2.453699688060887 1108 74-75 1.3861873281196253 1108 76 1.5585020922971466 1109 1 0.3060181167951015 1109 2 -0.9149420570255451 1109 3 -0.2267405598097305 1109 4 1.3449626585869119 1109 5 0.24723760214680368 1109 6 -1.0589455716268361 1109 7 -1.7494705752604638 1109 8 -0.4520970517447225 1109 9 1.3019903970977253 1109 10-11 0.6100531867744081 1109 12-13 -0.4380659245358913 1109 14-15 -1.0809920363969354 1109 16-17 0.2297526935100933 1109 18-19 -0.33674096458339164 1109 20-21 0.8742664645852045 1109 22-23 0.8518112647652387 1109 24-25 0.12303563427706621 1109 26-27 1.0112484412466713 1109 28-29 -0.15734520003350383 1109 30-31 -0.14287347834081032 1109 32-33 0.45479085546638487 1109 34-35 -0.24994520476326443 1109 36-37 -0.3550830361284838 1109 38-39 -0.18643658630313809 1109 40-41 0.5116209100536722 1109 42-43 0.7412581897449044 1109 44-45 0.12637314185511173 1109 46-47 -0.3422106139805905 1109 48-49 -0.15900246798608464 1109 50-51 0.7328364892360923 1109 52-53 1.251019217804206 1109 54-55 -0.719427246562141 1109 56-57 -0.2604081579849904 1109 58-59 -0.5718298737902998 1109 60-61 -1.3911609875921904 1109 62-63 -2.0424989733827488 1109 64-65 -0.39726615865562565 1109 66-67 0.1452238164651618 1109 68-69 0.8286382235055569 1109 70-71 -0.29079892851147093 1109 72-73 -0.29048635845074244 1109 74-75 0.7815361653289301 1109 76 -0.8601025588656412 1110 1 0.46105687648502425 1110 2 -0.9155383599474298 1110 3 -2.2374740124036485 1110 4 -1.4099568405186318 1110 5 -0.14453341753119275 1110 6 -1.5431435441969015 1110 7 -2.0392737952962428 1110 8 1.3296560788456162 1110 9 -1.147025703081166 1110 10-11 -0.6305550422059127 1110 12-13 0.17105751016893578 1110 14-15 -1.4211828533319384 1110 16-17 -0.07555440249467438 1110 18-19 -0.5201041130628212 1110 20-21 0.40974648843731387 1110 22-23 0.32467948181949424 1110 24-25 1.1281042091130828 1110 26-27 -0.4261397519554819 1110 28-29 0.41301854474884436 1110 30-31 0.14752604461686047 1110 32-33 0.9196089830752143 1110 34-35 0.48052587454502316 1110 36-37 0.5584530401982875 1110 38-39 0.32548947213454227 1110 40-41 0.36612299711390506 1110 42-43 -0.15170543577686146 1110 44-45 1.7158185801377641 1110 46-47 -0.04465545596031717 1110 48-49 -0.17003407204094856 1110 50-51 0.5214471034281019 1110 52-53 -0.30562955977480044 1110 54-55 0.8580921332828169 1110 56-57 0.6668428855451154 1110 58-59 -3.4797010723591697 1110 60-61 0.029828875258132825 1110 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3.3548721085181583 2116 64-65 2.579478027390884 2116 66-67 2.434910368537963 2116 68-69 1.7517929252244713 2116 70-71 -0.13508608725767957 2116 72-73 3.321242660760582 2116 74-75 1.8047919792824132 2116 76 -0.4061086255997175 2117 1 -1.2965188810907335 2117 2 -2.386400830809901 2117 3 -2.1675439424735785 2117 4 -1.4309358614976517 2117 5 -2.682994955992733 2117 6 -1.8834698845232438 2117 7 -0.923889179911626 2117 8 -4.142371893182354 2117 9 -2.5701026261580893 2117 10-11 -2.2144711261219925 2117 12-13 -2.070201231089804 2117 14-15 -2.798222480371564 2117 16-17 -2.566230393170663 2117 18-19 -3.060313903272611 2117 20-21 -2.210300131609305 2117 22-23 -3.3722902151501977 2117 24-25 -2.8468375158286463 2117 26-27 -3.0764894023051284 2117 28-29 -2.0252098934795946 2117 30-31 -3.0424506453598354 2117 32-33 -2.565822252356025 2117 34-35 -2.3947655007463524 2117 36-37 -3.8302415984963503 2117 38-39 -3.7118065651614884 2117 40-41 -2.387956256965353 2117 42-43 -1.40461918869061 2117 44-45 -3.245719881400703 2117 46-47 -3.607009768314633 2117 48-49 -2.3809897829966573 2117 50-51 -1.558389726408727 2117 52-53 -1.810291564436806 2117 54-55 -0.838877563686875 2117 56-57 -1.89201492331269 2117 58-59 -4.14054023319833 2117 60-61 -1.927630332201069 2117 62-63 -2.6312938993869786 2117 64-65 -1.6705219726091158 2117 66-67 -2.208370263873107 2117 68-69 -1.1829896834711846 2117 70-71 -2.5471414232260585 2117 72-73 -3.153065639634672 2117 74-75 -3.945208020717587 2117 76 -3.5049228548487292 2118 1 2.394390209818358 2118 2 1.8908718964628228 2118 3 0.6279106029809682 2118 4 0.9054277748659842 2118 5 2.067005044007267 2118 6 3.1119846609313058 2118 7 2.076110820088374 2118 8 -0.8241900750005371 2118 9 2.729897373841908 2118 10-11 0.4059834193325571 2118 12-13 2.172980587092013 2118 14-15 2.03586842871934 2118 16-17 0.4564968795566102 2118 18-19 0.5010497330910226 2118 20-21 2.053336232027064 2118 22-23 1.7663461484861642 2118 24-25 1.8486170296259061 2118 26-27 2.850783324967601 2118 28-29 1.7566082883385832 2118 30-31 1.4757311728219875 2118 32-33 2.89781411128034 2118 34-35 2.9029617719809195 2118 36-37 2.2629402196854684 2118 38-39 2.4722843439294238 2118 40-41 2.180225561216467 2118 42-43 0.7726535385821194 2118 44-45 0.1542801185992957 2118 46-47 1.5316265953217325 2118 48-49 2.248555671548793 2118 50-51 1.9938830008640025 2118 52-53 0.5533447991995573 2118 54-55 3.424758799949487 2118 56-57 3.7988941675963943 2118 58-59 2.8299143122562143 2118 60-61 0.2791878496171165 2118 62-63 2.8255242824312106 2118 64-65 1.8544780273908898 2118 66-67 0.6870842815814413 2118 68-69 1.7920103165288097 2118 70-71 1.7301313040466653 2118 72-73 1.4060252694562436 2118 74-75 3.554791979282413 2118 76 1.217804417878547 2119 1 -0.1431097901816436 2119 2 0.4658718964628257 2119 3 1.0029106029809682 2119 4 0.830427774865985 2119 5 1.0670050440072671 2119 6 0.7244846609313029 2119 7 0.201110820088374 2119 8 2.988309924999463 2119 9 1.9298973738419107 2119 10-11 0.20598341933255426 2119 12-13 1.060480587092016 2119 14-15 1.9546184287193427 2119 16-17 -0.5435031204433898 2119 18-19 1.6385497330910255 2119 20-21 -1.1591637679729416 2119 22-23 0.5850961484861656 2119 24-25 0.8548670296259004 2119 26-27 0.5257833249675983 2119 28-29 0.43785828833858886 2119 30-31 -0.8180188271780153 2119 32-33 0.7978141112803385 2119 34-35 0.877961771980921 2119 36-37 1.5504402196854699 2119 38-39 0.16603434392941807 2119 40-41 0.8989755612164672 2119 42-43 0.10390353858211654 2119 44-45 -2.526969881400703 2119 46-47 0.7253765953217339 2119 48-49 0.19855567154879594 2119 50-51 0.38763300086399965 2119 52-53 -2.4154052008004427 2119 54-55 -0.6002412000505117 2119 56-57 -0.2698558324036 2119 58-59 0.5924143122562171 2119 60-61 -0.05831215038288917 2119 62-63 0.3005242824312049 2119 64-65 -0.014271972609115835 2119 66-67 -0.06916571841856012 2119 68-69 -0.5579896834711846 2119 70-71 0.055131304046668106 2119 72-73 -1.118974730543762 2119 74-75 -0.6952080207175868 2119 76 1.9553044178785441 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 4.0 13 44.0 14 106.0 15 193.0 16 258.0 17 281.0 18 289.0 19 270.0 20 204.0 21 185.0 22 128.0 23 92.0 24 88.0 25 100.0 26 114.0 27 130.0 28 160.0 29 203.0 30 262.0 31 314.0 32 413.0 33 604.0 34 945.0 35 1517.0 36 2697.0 37 3802.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.328806182936894 23.598709386958806 13.048698131612516 29.023786298491782 2 16.809669476982762 24.397522942624786 34.57434902633739 24.21845855405506 3 14.474371409385958 29.903752891143775 29.29194956353055 26.32992613593971 4 10.922927702753114 20.704319928374247 37.2080877415504 31.164664627322242 5 11.20644631798851 37.066328433932696 35.79049466537342 15.936730582705364 6 23.755875550249943 39.259867193911816 20.23427590837872 16.749981347459524 7 23.569350145489814 33.984928747295385 21.8010893083638 20.644631798851005 8 20.861001268372753 36.088935312989626 20.383496232186822 22.666567186450795 9 21.35342833693949 17.212564351264643 22.29351637693054 39.140490934865326 10-11 22.185331642169665 26.11728717451317 28.075803924494515 23.62157725882265 12-13 20.819965679325524 23.912556890248453 27.389390434977244 27.878086995448783 14-15 18.645079459822426 26.027754980228305 25.777810937849736 29.54935462209953 16-17 21.301201223606654 28.75102588972618 26.050138028799523 23.89763485786764 18-19 20.883384316943967 26.997687084980974 25.919570245467433 26.199358352607625 20-21 20.103708125046634 27.243900619264345 25.546519435947175 27.10587181974185 22-23 21.26389614265463 26.58733119450869 26.58733119450869 25.56144146832799 24-25 20.898306349324777 28.586883533537268 25.56517197642319 24.949638140714768 26-27 19.514287846004628 29.15765127210326 26.490337984033424 24.83772289785869 28-29 21.081101245989704 27.781093784973514 27.281205700216372 23.856599268820414 30-31 20.622248750279788 27.870625979258374 26.97530403640976 24.531821234052078 32-33 19.656047153622325 29.47101395210028 26.620905767365517 24.252033126911883 34-35 20.57748265313736 28.27352085354025 26.952920987838542 24.196075505483847 36-37 21.025143624561665 27.587107364022977 26.99022606879057 24.397522942624786 38-39 21.096023278370517 27.840781914496755 27.430426024024474 23.63276878310826 40-41 20.431992837424456 27.079758263075433 27.818398865925538 24.669850033574573 42-43 19.398642095053344 27.437887040214875 28.150414086398566 25.01305677833321 44-45 19.31284040886369 26.732821010221592 28.385436096396326 25.56890248451839 46-47 19.659777661717527 27.45653958069089 27.986271730209655 24.89741102738193 48-49 18.988286204581065 28.12430052973215 27.74751921211669 25.139894053570096 50-51 19.723196299335967 27.195404014026707 27.665448034022234 25.41595165261509 52-53 18.816682832201746 27.508766694023727 27.878086995448783 25.796463478325748 54-55 18.066850705066027 27.695292098783852 27.975080205924048 26.262776990226065 56-57 18.80549130791614 27.616951428784603 27.680370066403043 25.897187196896216 58-59 19.249421771245242 27.39685145116765 27.13198537640827 26.22174140117884 60-61 18.61896590315601 26.647019324031934 27.53861075878535 27.195404014026707 62-63 18.876370961724987 26.982765052600165 27.736327687831082 26.404536297843766 64-65 18.78310825934492 27.56099380735656 26.911885398791313 26.7440125345072 66-67 19.342684473625308 27.549802283070957 26.303812579273295 26.803700664030437 68-69 18.742072670297695 28.247407296873835 26.22174140117884 26.788778631649627 70-71 19.577706483623068 28.236215772588224 25.098858464522866 27.087219279265835 72-73 19.816459001716034 27.262553159740357 25.483100798328735 27.437887040214875 74-75 20.017906438856972 27.594568380213385 25.453256733567116 26.93426844736253 76 19.480713273147803 26.702976945459973 24.82280086547788 28.993508915914347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 3.0 18 3.0 19 3.5 20 7.5 21 11.0 22 8.5 23 12.0 24 14.5 25 11.0 26 26.5 27 47.5 28 65.5 29 78.0 30 94.0 31 114.5 32 158.5 33 198.0 34 222.5 35 320.0 36 383.5 37 374.0 38 464.0 39 630.0 40 751.0 41 849.0 42 902.0 43 904.5 44 956.5 45 988.0 46 970.0 47 1002.5 48 1001.0 49 895.0 50 823.0 51 789.0 52 639.5 53 496.0 54 468.0 55 431.5 56 344.0 57 256.0 58 219.0 59 167.0 60 100.5 61 77.5 62 69.0 63 56.5 64 32.0 65 21.0 66 17.0 67 12.0 68 11.0 69 7.5 70 6.5 71 8.0 72 5.5 73 3.5 74 2.0 75 0.0 76 1.5 77 2.5 78 1.0 79 0.0 80 0.0 81 1.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 1.0 93 0.5 94 1.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5670372304707901 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13403.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.35715884503469 #Duplication Level Percentage of deduplicated Percentage of total 1 77.11208699289 55.02499440423786 2 13.414889167712255 19.144967544579572 3 5.40568799665412 11.572036111318361 4 2.0911752404851525 5.9688129523241065 5 1.0455876202425762 3.730508095202566 6 0.533249686323714 2.283070954263971 7 0.1672940192388122 0.835633813325375 8 0.1150146382266834 0.6565694247556517 9 0.07319113341698034 0.4700440199955234 >10 0.04182350480970305 0.3133626799970156 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0074610161904051335 0.0 3 0.0 0.0 0.0 0.0074610161904051335 0.0 4 0.0 0.0 0.0 0.0074610161904051335 0.0 5 0.0 0.0 0.0 0.0074610161904051335 0.0 6 0.0 0.0 0.0 0.014922032380810267 0.0 7 0.0 0.0 0.0 0.014922032380810267 0.0 8 0.0 0.0 0.0 0.014922032380810267 0.0 9 0.0 0.0 0.0 0.0223830485712154 0.0 10 0.0 0.0 0.0 0.0223830485712154 0.0 11 0.0 0.0 0.0 0.029844064761620534 0.0 12 0.0 0.0 0.0 0.037305080952025664 0.0 13 0.0 0.0 0.0 0.037305080952025664 0.0 14 0.0 0.0 0.0 0.037305080952025664 0.0 15 0.0 0.0 0.0 0.037305080952025664 0.0 16 0.0 0.0 0.0 0.037305080952025664 0.0 17 0.0 0.0 0.0 0.0447660971424308 0.0 18 0.0 0.0 0.0 0.0671491457136462 0.0 19 0.0 0.0 0.0 0.08207117809445647 0.0 20 0.0 0.0 0.0 0.0895321942848616 0.0 21 0.0 0.0 0.0 0.10445422666567186 0.0 22 0.0 0.0 0.0 0.111915242856077 0.0 23 0.0 0.0 0.0 0.11937625904648214 0.0 24 0.0 0.0 0.0 0.11937625904648214 0.0 25 0.0 0.0 0.0 0.14922032380810266 0.0 26 0.0 0.0 0.0 0.14922032380810266 0.0 27 0.0 0.0 0.0 0.16414235618891293 0.0 28 0.0 0.0 0.0 0.17160337237931805 0.0 29 0.0 0.0 0.0 0.2014474371409386 0.0 30 0.0 0.0 0.0 0.23129150190255912 0.0 31 0.0 0.0 0.0 0.23875251809296427 0.0 32 0.0 0.0 0.0 0.27605759904498994 0.0 33 0.0 0.0 0.0 0.3133626799970156 0.0 34 0.0 0.0 0.0 0.3581287771394464 0.0 35 0.0 0.0 0.0 0.4103558904722823 0.0 36 0.0 0.0 0.0 0.5148101171379542 0.0 37 0.0 0.0 0.0 0.671491457136462 0.0 38 0.0 0.0 0.0 0.8878609266582108 0.0 39 0.0 0.0 0.0 1.1788405580840111 0.0 40 0.0 0.0 0.0 1.5369693352234575 0.0 41 0.0 0.0 0.0 1.8652540476012833 0.0 42 0.0 0.0 0.0 2.3576811161680222 0.0 43 0.0 0.0 0.0 2.7232709094978738 0.0 44 0.0 0.0 0.0 3.1485488323509663 0.0 45 0.0 0.0 0.0 3.640975900917705 0.0 46 0.0 0.0 0.0 4.088636872342013 0.0 47 0.0 0.0 0.0 4.588524957099157 0.0 48 0.0 0.0 0.0 5.1630232037603525 0.0 49 0.0 0.0 0.0 5.752443482802358 0.0 50 0.0 0.0 0.0 6.200104454226666 0.0 51 0.0 0.0 0.0 6.737297619935835 0.0 52 0.0 0.0 0.0 7.170036558979333 0.0 53 0.0 0.0 0.0 7.699768708498097 0.0 54 0.0 0.0 0.0 8.192195777064835 0.0 55 0.0 0.0 0.0 8.729388942774007 0.0 56 0.0 0.0 0.0 9.50533462657614 0.0 57 0.0 0.0 0.0 9.960456614190853 0.0 58 0.0 0.0 0.0 10.549876893232858 0.0 59 0.0 0.0 0.0 11.116914123703648 0.0 60 0.0 0.0 0.0 11.72871745131687 0.0 61 0.0 0.0 0.0 12.243527568454823 0.0 62 0.0 0.0 0.0 12.788181750354399 0.0 63 0.0 0.0 0.0 13.21345967320749 0.0 64 0.0 0.0 0.0 13.825263000820712 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATCCA 25 2.3077066E-4 55.99403 2 TAACCTA 20 0.0068066525 52.4944 63 TAAAGGA 35 0.0012125231 39.995735 5 >>END_MODULE