FastQCFastQC Report
Wed 25 May 2016
SRR1780728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13403
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3992.976945459971648RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2932.186077743788704No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC470.3506677609490413No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC430.32082369618742074No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG320.23875251809296424No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC320.23875251809296424No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT290.21636946952174885No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG210.1566813399985078No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT200.14922032380810266No Hit
CTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTG180.1342982914272924No Hit
ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT170.1268372752368873RNA PCR Primer, Index 15 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT160.11937625904648212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCT150.00217709570.0000168
CTCTGTT150.00217709570.0000121
AGTTCTT200.006803781752.50000469
AAGTTAA1703.6379788E-1226.76470628
AATCTCG1507.003109E-1025.66666633
CCGTCTT1507.003109E-1025.66666644
GTCTTCT1507.003109E-1025.66666646
TCTTCTG1507.003109E-1025.66666647
CGTCTTC1507.003109E-1025.66666645
GTTAATC1507.003109E-1025.66666630
AGTTAAT1507.003109E-1025.66666629
TTAATCT1657.2759576E-1125.45454631
ATAAGTT1807.2759576E-1225.27777726
CTCGTAT1807.2759576E-1225.27777736
ATCTCGT1551.02591E-924.83870934
GAAAAAA1551.02591E-924.83870957
TAATCTC1551.02591E-924.83870932
GCCGTCT1551.02591E-924.83870943
CTTGAAA1551.02591E-924.83870954
CTTCTGC1551.02591E-924.83870948
Produced by FastQC (version 0.11.5)