##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780728_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13403 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.346713422368126 34.0 34.0 34.0 27.0 34.0 2 33.10587181974185 34.0 34.0 34.0 32.0 34.0 3 33.29896291874953 34.0 34.0 34.0 33.0 34.0 4 33.221666791016936 34.0 34.0 34.0 33.0 34.0 5 33.24651197493098 34.0 34.0 34.0 33.0 34.0 6 36.93083637991494 38.0 37.0 38.0 35.0 38.0 7 36.571513840185034 38.0 37.0 38.0 34.0 38.0 8 36.47474446019548 38.0 37.0 38.0 34.0 38.0 9 36.330821457882564 38.0 37.0 38.0 34.0 38.0 10-11 35.972431545176455 38.0 36.5 38.0 32.5 38.0 12-13 35.84716108333956 38.0 36.0 38.0 31.0 38.0 14-15 36.135305528613 38.0 36.5 38.0 33.0 38.0 16-17 36.27519958218309 38.0 37.0 38.0 34.0 38.0 18-19 36.252070431992834 38.0 37.0 38.0 34.0 38.0 20-21 35.99847049168097 38.0 36.5 38.0 32.5 38.0 22-23 36.00951279564276 38.0 36.5 38.0 32.5 38.0 24-25 35.132544952622546 37.5 35.0 38.0 29.0 38.0 26-27 35.43042602402447 37.5 36.0 38.0 31.0 38.0 28-29 35.18857718421249 37.5 35.5 38.0 29.0 38.0 30-31 34.84682533761098 37.0 34.5 38.0 27.0 38.0 32-33 35.352794150563305 37.5 36.0 38.0 31.0 38.0 34-35 35.29706035962098 37.5 36.0 38.0 29.0 38.0 36-37 35.526710437961654 38.0 36.0 38.0 31.0 38.0 38-39 35.13243303737969 37.5 35.5 38.0 29.0 38.0 40-41 34.60512571812281 37.0 34.5 38.0 26.5 38.0 42-43 35.226516451540704 37.0 35.5 38.0 29.0 38.0 44-45 35.44840707304335 38.0 36.0 38.0 31.0 38.0 46-47 35.608744310975155 38.0 36.0 38.0 31.0 38.0 48-49 35.33246288144446 37.5 36.0 38.0 29.0 38.0 50-51 35.53655897933299 38.0 36.0 38.0 31.0 38.0 52-53 35.43426844736253 38.0 36.0 38.0 31.0 38.0 54-55 35.6325822577035 38.0 36.0 38.0 31.0 38.0 56-57 35.743900619264345 38.0 36.0 38.0 31.5 38.0 58-59 35.76956651495934 38.0 37.0 38.0 31.5 38.0 60-61 35.344288592106246 38.0 36.0 38.0 29.0 38.0 62-63 35.676751473550695 38.0 36.0 38.0 31.0 38.0 64-65 35.68827874356487 38.0 36.5 38.0 31.5 38.0 66-67 34.8684249794822 37.5 35.5 38.0 27.0 38.0 68-69 33.81444452734463 37.0 34.0 38.0 23.0 38.0 70-71 33.53241811534731 37.0 34.0 38.0 22.0 38.0 72-73 32.9428486159815 37.0 32.5 38.0 20.0 38.0 74-75 33.67335671118407 37.0 34.0 38.0 22.0 38.0 76 33.189957472207716 37.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8074969178802647 1101 2 0.8043751766080192 1101 3 0.6297455430955026 1101 4 0.2422992811729472 1101 5 0.0038254762092293504 1101 6 -1.6042955685076166 1101 7 -0.793426533149983 1101 8 0.1546129747689733 1101 9 -0.24014863992341162 1101 10-11 0.4540584219637793 1101 12-13 0.9737507595779249 1101 14-15 0.4509352692775721 1101 16-17 0.4877707831036062 1101 18-19 -0.25403118712155504 1101 20-21 0.029804619458175807 1101 22-23 0.7558974205539926 1101 24-25 -0.9414088046429896 1101 26-27 0.3412059340391096 1101 28-29 0.921263176449969 1101 30-31 1.3361573540303127 1101 32-33 0.808190796614376 1101 34-35 1.5370446667282636 1101 36-37 0.21209935087657072 1101 38-39 1.6923391698862602 1101 40-41 1.8058701893535414 1101 42-43 1.7811522800451485 1101 44-45 1.3242926946946554 1101 46-47 0.6513889898556897 1101 48-49 -0.8752450643768768 1101 50-51 -1.123206022141602 1101 52-53 -0.8635401559483356 1101 54-55 1.5555503367892243E-4 1101 56-57 0.30747816267226824 1101 58-59 -1.062454279060816 1101 60-61 -1.7868240608894794 1101 62-63 0.06455817385960216 1101 64-65 -0.9482465200799766 1101 66-67 -2.695209689626104 1101 68-69 -0.8941344777067357 1101 70-71 -1.2237284077729456 1101 72-73 -0.7084910167113989 1101 74-75 0.9104654385399868 1101 76 -0.1200589228989628 1102 1 -0.4029773904201335 1102 2 0.4575372319439879 1102 3 0.576385859301034 1102 4 0.21957200844567382 1102 5 0.24097962640685466 1102 6 0.2731747872236099 1102 7 0.2589450083519935 1102 8 0.5508580340575122 1102 9 0.4930529411042599 1102 10-11 0.2628528883669432 1102 12-13 0.9292843564158773 1102 14-15 0.13176530880325998 1102 16-17 -0.3867351457501442 1102 18-19 -0.4615410685444772 1102 20-21 0.8415635127388086 1102 22-23 0.4283282505935233 1102 24-25 -5.357416709781326 1102 26-27 -0.6597822082533824 1102 28-29 0.21869400648949977 1102 30-31 1.4715328481014538 1102 32-33 0.8052263697369 1102 34-35 1.054831227993084 1102 36-37 -2.127327526593781 1102 38-39 0.6305802766056203 1102 40-41 1.56575161227844 1102 42-43 0.9945910152230084 1102 44-45 0.9339764891610542 1102 46-47 2.022436420685729 1102 48-49 -2.8515296493571114 1102 50-51 -0.30057756364357147 1102 52-53 1.4408076701386179 1102 54-55 1.1157682032550227 1102 56-57 0.8731896251228619 1102 58-59 1.1934745746941289 1102 60-61 -3.9829703059487684 1102 62-63 -1.3267461739664839 1102 64-65 1.0616349028449221 1102 66-67 2.486608492192083 1102 68-69 0.13452164877547546 1102 70-71 -0.3872659571800554 1102 72-73 -5.062245957422867 1102 74-75 -0.0910167748987476 1102 76 -0.010375128432556835 1103 1 1.2869883042625432 1103 2 0.6895269403487916 1103 3 0.19988500166810041 1103 4 0.38380871513520987 1103 5 0.6920945492198953 1103 6 0.6196584921978854 1103 7 1.044965591542386 1103 8 0.7202405383456707 1103 9 0.5728128038829894 1103 10-11 0.6322782743017612 1103 12-13 -0.15176195576251672 1103 14-15 0.12361779593877031 1103 16-17 0.6089766895843312 1103 18-19 0.25458243059787833 1103 20-21 -0.394928768564796 1103 22-23 0.9529851974202757 1103 24-25 -1.8222537595240311 1103 26-27 -0.23139455816762222 1103 28-29 -0.39108300894790204 1103 30-31 0.3484625222009399 1103 32-33 1.8073704520696623 1103 34-35 1.353287488713498 1103 36-37 -0.6506980240208833 1103 38-39 0.6029216145130007 1103 40-41 -0.2888796055603251 1103 42-43 -0.6456319693395969 1103 44-45 1.6754001598300121 1103 46-47 1.5024964549910536 1103 48-49 -2.628116270283357 1103 50-51 -0.28235286381509894 1103 52-53 0.9219397456103167 1103 54-55 0.5415829545414752 1103 56-57 0.39012787555166994 1103 58-59 -0.06553057110347282 1103 60-61 -4.627267046943626 1103 62-63 -1.4776895701928936 1103 64-65 0.7022867038740799 1103 66-67 0.8802620084871009 1103 68-69 0.44712885289211357 1103 70-71 0.4071165471081031 1103 72-73 -4.590976660681868 1103 74-75 -0.9059824695814243 1103 76 -2.1085740992730386 1104 1 -0.2420682995110397 1104 2 0.8348099592167131 1104 3 0.6036585865737578 1104 4 0.4222992811729469 1104 5 -0.7935658281385969 1104 6 0.47135660540543256 1104 7 1.0343995538065371 1104 8 0.2972216704211448 1104 9 0.8094165774678928 1104 10-11 -0.8394198389057834 1104 12-13 0.022011629143143807 1104 14-15 0.26267439971235973 1104 16-17 0.6469012178862172 1104 18-19 1.0711862041827942 1104 20-21 -0.8888910327157404 1104 22-23 0.7824191596844301 1104 24-25 -0.1492348915995052 1104 26-27 1.2329450644738955 1104 28-29 2.3877849155804114 1104 30-31 1.3978964844650932 1104 32-33 0.5134081879187207 1104 34-35 1.2157403189021778 1104 36-37 2.009490655224397 1104 38-39 1.8410348220601662 1104 40-41 2.738478885005712 1104 42-43 0.30550010613210077 1104 44-45 2.1012492164337786 1104 46-47 2.17834551159482 1104 48-49 1.3230158051883407 1104 50-51 1.4089678909018772 1104 52-53 1.460807670138614 1104 54-55 1.0666772941641156 1104 56-57 0.6518259887592208 1104 58-59 -1.1428890616695142 1104 60-61 1.368393330414868 1104 62-63 0.5732538260335147 1104 64-65 1.244362175572192 1104 66-67 0.2947903103738909 1104 68-69 2.9054307396845687 1104 70-71 1.5640976791835755 1104 72-73 -0.4858823210592291 1104 74-75 -1.9069258658078425 1104 76 -1.7931024011598318 1105 1 0.8300885632340567 1105 2 0.4838295670598498 1105 3 0.4507174101031737 1105 4 0.15406398705530222 1105 5 -0.25278151441310825 1105 6 0.326650723052488 1105 7 0.7504779851790886 1105 8 -0.36669989820630633 1105 9 0.9235342245267191 1105 10-11 0.2782272199177527 1105 12-13 -0.6166158218372502 1105 14-15 0.7569881252025468 1105 16-17 0.3494502374940609 1105 18-19 0.28177443947690506 1105 20-21 0.5436579868921072 1105 22-23 0.4526152381158042 1105 24-25 0.5784121672240232 1105 26-27 1.304121535062123 1105 28-29 1.049353543031387 1105 30-31 0.13240628838666169 1105 32-33 1.0008591683108747 1105 34-35 -1.1973969359997838 1105 36-37 1.0422357532636113 1105 38-39 0.7029956063738965 1105 40-41 0.8237730026527714 1105 42-43 0.07079422377915989 1105 44-45 1.1171315693749548 1105 46-47 1.6108945312026677 1105 48-49 -0.4999253712822451 1105 50-51 1.313281616392075 1105 52-53 1.6074743368052893 1105 54-55 1.1723635686739229 1105 56-57 1.234375008367067 1105 58-59 1.312012899114805 1105 60-61 0.8176090166893744 1105 62-63 -0.02282460533903219 1105 64-65 1.0206366853761182 1105 66-67 0.6479275652758574 1105 68-69 -0.730451613256605 1105 70-71 2.1240976791835777 1105 72-73 1.5398039534505799 1105 74-75 0.35483884007450683 1105 76 0.4178779909970345 1106 1 -0.5853241134645266 1106 2 0.28364716851903893 1106 3 0.19900742378306546 1106 4 0.3469504439636424 1106 5 0.3678295206986135 1106 6 0.2541473030798471 1106 7 -1.3758330043329963 1106 8 0.43722167042114535 1106 9 -1.0682578411367558 1106 10-11 -0.39755937378950534 1106 12-13 1.3124767454222166 1106 14-15 -0.12034885610160018 1106 16-17 0.19783145044435457 1106 18-19 0.008860622787437933 1106 20-21 0.6827368742610034 1106 22-23 0.4275354387541981 1106 24-25 -1.4578395427623008 1106 26-27 1.0141078551715665 1106 28-29 1.2440639853478501 1106 30-31 1.0206871821395183 1106 32-33 -0.4638011144068557 1106 34-35 1.9015542723905483 1106 36-37 1.4585604226662596 1106 38-39 0.5917324964787696 1106 40-41 1.7280137687266404 1106 42-43 0.7889884782251215 1106 44-45 1.9577608443407613 1106 46-47 1.494159465083193 1106 48-49 1.6204576656534542 1106 50-51 0.9010609141576964 1106 52-53 0.7896448794409494 1106 54-55 0.39853775928039425 1106 56-57 0.24554691899177783 1106 58-59 1.0919946592607275 1106 60-61 1.1272305397171962 1106 62-63 1.731393360917238 1106 64-65 -1.2121494523347849 1106 66-67 1.8164182173506376 1106 68-69 3.7312446931729397 1106 70-71 2.810144190811485 1106 72-73 1.2427223301035681 1106 74-75 0.9430741341921589 1106 76 1.3157348081424942 1107 1 0.9272934026166197 1107 2 0.4880014485784159 1107 3 0.6972756078503579 1107 4 0.5933631109601833 1107 5 -0.022076466436473652 1107 6 0.474335328809687 1107 7 1.3203570006150542 1107 8 0.5223280533998675 1107 9 0.33154423704235825 1107 10-11 1.3839844164133694 1107 12-13 1.2286073738239907 1107 14-15 0.6428871656697979 1107 16-17 1.3705182391628128 1107 18-19 1.4920372680125809 1107 20-21 1.5860025843055396 1107 22-23 1.0262489469184715 1107 24-25 1.4394885126558137 1107 26-27 0.8842216602185715 1107 28-29 1.6384232134527466 1107 30-31 1.740875207869344 1107 32-33 2.532982656003824 1107 34-35 1.1776552125191984 1107 36-37 1.8603417190541904 1107 38-39 1.9286943965282504 1107 40-41 1.7161384594737967 1107 42-43 1.5376277657065671 1107 44-45 1.8048662377103781 1107 46-47 2.227707213722482 1107 48-49 2.0764200605074947 1107 50-51 1.614074273880604 1107 52-53 2.4195310743939373 1107 54-55 1.2758262303343315 1107 56-57 1.521400456844333 1107 58-59 1.6209407255645303 1107 60-61 2.3422231176489063 1107 62-63 1.9179346770973495 1107 64-65 1.9832983457849593 1107 66-67 1.187769033778146 1107 68-69 3.624366909897333 1107 70-71 1.2730338493963416 1107 72-73 2.749649593834384 1107 74-75 1.2622230703623671 1107 76 1.8392380243720865 1108 1 0.15886193304709906 1108 2 0.8417867034027608 1108 3 0.19900742378306546 1108 4 -0.0716542071991455 1108 5 0.5073644044195404 1108 6 0.23089148912635693 1108 7 0.6009111817135135 1108 8 0.04187283321184054 1108 9 0.6991840193283565 1108 10-11 0.33499876574538234 1108 12-13 1.2310813965850045 1108 14-15 1.0773255625030487 1108 16-17 0.546668659746679 1108 18-19 0.7530466692990672 1108 20-21 1.5083182696098447 1108 22-23 0.20660520619605904 1108 24-25 2.1700674339818846 1108 26-27 -0.7649619122702944 1108 28-29 -0.616401130931223 1108 30-31 1.0671988100464844 1108 32-33 1.6989895832675543 1108 34-35 1.296903109599853 1108 36-37 1.1097232133639352 1108 38-39 2.882430170897372 1108 40-41 1.2163858617498988 1108 42-43 -0.17612780084463964 1108 44-45 0.5972957280616882 1108 46-47 1.110438534850637 1108 48-49 1.5390623168162492 1108 50-51 1.4475725420646768 1108 52-53 0.2547611585107106 1108 54-55 0.1194679918385333 1108 56-57 0.8153143608522413 1108 58-59 0.8478086127490911 1108 60-61 2.0109514699497524 1108 62-63 1.6848817330102577 1108 64-65 0.8925017104559174 1108 66-67 1.9210693801413399 1108 68-69 2.196360972242708 1108 70-71 -0.08520464639781977 1108 72-73 0.5799316324291439 1108 74-75 0.303539250471232 1108 76 2.432013877909938 1109 1 -1.3062543460226692 1109 2 0.5394611220074097 1109 3 0.4780771912249264 1109 4 0.6957876532659739 1109 5 0.5306202183730306 1109 6 -0.5365503713387554 1109 7 -0.21304230665857915 1109 8 0.8558263215839332 1109 9 0.8619747170027736 1109 10-11 0.6838359750476997 1109 12-13 0.6729418617012897 1109 14-15 1.3098837020379364 1109 16-17 1.8606221481187717 1109 18-19 1.2646745762758087 1109 20-21 1.5780857114703082 1109 22-23 0.4856749736379129 1109 24-25 0.39099766654003076 1109 26-27 0.5257357621483152 1109 28-29 -0.639656944884706 1109 30-31 0.7997569495813721 1109 32-33 1.37340818791872 1109 34-35 0.4829496212277604 1109 36-37 2.028327864526723 1109 38-39 2.835918542990399 1109 40-41 1.181502140819667 1109 42-43 1.8820117340390823 1109 44-45 1.6786910768989003 1109 46-47 2.0057873720599346 1109 48-49 1.655341386583693 1109 50-51 1.1103632397390868 1109 52-53 0.022203018975822886 1109 54-55 0.3636540383501625 1109 56-57 0.22229110503829475 1109 58-59 0.8826923336793229 1109 60-61 1.4760677490195135 1109 62-63 1.0918584771963111 1109 64-65 1.6483156639442882 1109 66-67 0.804790310373896 1109 68-69 0.7079888792194495 1109 70-71 1.8799116326719485 1109 72-73 2.138071167312866 1109 74-75 0.8267950644247151 1109 76 1.6878278313983088 1110 1 -1.613350350793091 1110 2 -0.80877978437303 1110 3 -0.01685423393905694 1110 4 0.3833249221985895 1110 5 0.606946992374219 1110 6 -0.1650536510048255 1110 7 0.47747647688346007 1110 8 1.0013242345237074 1110 9 1.3473652954166084 1110 10-11 -0.3137788132647614 1110 12-13 0.2853449624764721 1110 14-15 1.216264143302098 1110 16-17 0.46408070506570454 1110 18-19 0.4778528708494605 1110 20-21 0.6198269160022036 1110 22-23 -0.6016834044181394 1110 24-25 -0.8597477121123234 1110 26-27 1.6449963465251756 1110 28-29 0.4354772232727129 1110 30-31 -0.42415479758619057 1110 32-33 1.4246902392007712 1110 34-35 1.5175351906970533 1110 36-37 1.897439373173114 1110 38-39 1.3394963605217072 1110 40-41 -1.3987006021737756 1110 42-43 0.386782157414153 1110 44-45 0.6420184472030073 1110 46-47 0.4819352551845668 1110 48-49 2.0196824718550133 1110 50-51 -0.6248782629442715 1110 52-53 -0.2771410478100975 1110 54-55 -0.4920406545538327 1110 56-57 0.32336445029768157 1110 58-59 0.09557247679202163 1110 60-61 -1.3369912849697485 1110 62-63 -0.8011051483254619 1110 64-65 0.8343621755721955 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-2.8395508679060697 2116 66-67 -3.282166211365233 2116 68-69 -3.024569260315431 2116 70-71 0.863228113966187 2116 72-73 0.8675959398103359 2116 74-75 -1.1221432571121852 2116 76 1.1842889031879977 2117 1 -0.33479557223831335 2117 2 0.7984463228530814 2117 3 -0.6963414134262393 2117 4 -0.21224617337250606 2117 5 0.7864341718614014 2117 6 0.8186293326781566 2117 7 0.7362177356247201 2117 8 0.5735853067847785 2117 9 -2.1433106952593803 2117 10-11 -0.6121471116330568 2117 12-13 -0.5025338254023097 2117 14-15 0.7681289451669002 2117 16-17 -3.4549169639319643 2117 18-19 -0.9388137958172109 2117 20-21 -0.8061637599884648 2117 22-23 -0.1966717494064767 2117 24-25 -1.3346894370540525 2117 26-27 -1.7734185718897493 2117 28-29 -2.1222150844195937 2117 30-31 -0.16483078826217934 2117 32-33 -2.2175009029903734 2117 34-35 0.46392213708399055 2117 36-37 -1.9114184356846948 2117 38-39 0.8237620947874333 2117 40-41 -0.7751574786306534 2117 42-43 0.5627728334048285 2117 44-45 -1.5660235108389458 2117 46-47 -0.6934726702233647 2117 48-49 -0.7151660129934783 2117 50-51 -2.084668472734478 2117 52-53 0.5544440337749776 2117 54-55 -0.6342317967449773 2117 56-57 -0.047264920331684834 2117 58-59 -0.420161788942238 2117 60-61 0.16475696677850493 2117 62-63 -0.5085643557846709 2117 64-65 -0.9383650971550779 2117 66-67 -1.2406642350806507 2117 68-69 -4.638205623951798 2117 70-71 -3.5804477753618755 2117 72-73 -1.9486095937865002 2117 74-75 -2.2614713203532943 2117 76 0.3759885079310763 2118 1 -2.2120682995110386 2118 2 -0.4151900407832869 2118 3 -1.99634141342624 2118 4 -0.5077007188270528 2118 5 -0.16356582813860143 2118 6 -1.4586433945945672 2118 7 0.6543995538065417 2118 8 -0.3627783295788518 2118 9 -1.6705834225321041 2118 10-11 -2.0144198389057806 2118 12-13 -2.0979883708568536 2118 14-15 0.5226743997123506 2118 16-17 -7.798098782113783 2118 18-19 -2.4638137958172095 2118 20-21 -0.20389103271573816 2118 22-23 -0.06258084031556876 2118 24-25 0.43576510840049565 2118 26-27 -2.262054935526109 2118 28-29 -1.9222150844195909 2118 30-31 -1.392103515534906 2118 32-33 -1.426591812081277 2118 34-35 -2.704259681097824 2118 36-37 -4.170509344775603 2118 38-39 -0.8739651779398301 2118 40-41 -3.261521114994288 2118 42-43 -0.8644998938679009 2118 44-45 -3.9137507835662184 2118 46-47 -1.861654488405179 2118 48-49 -1.7969841948116567 2118 50-51 -1.421032109098121 2118 52-53 -2.9841923298613793 2118 54-55 -1.0183227058358781 2118 56-57 0.061825988759224515 2118 58-59 -2.1928890616695114 2118 60-61 -1.4216066695851381 2118 62-63 -3.401746173966483 2118 64-65 -3.065637824427803 2118 66-67 -1.2702096896261068 2118 68-69 0.600430739684569 2118 70-71 -0.2259023208164237 2118 72-73 3.024117678940776 2118 74-75 1.568074134192159 2118 76 -0.03310240115983021 2119 1 1.06293170048896 2119 2 -2.6276900407832855 2119 3 -2.1338414134262393 2119 4 -2.382700718827053 2119 5 -0.6510658281385986 2119 6 -3.47114339459457 2119 7 -2.6956004461934597 2119 8 -0.7502783295788547 2119 9 0.19191657746789303 2119 10-11 -0.7144198389057834 2119 12-13 -0.23548837085685648 2119 14-15 -0.21482560028764652 2119 16-17 -0.054348782113784466 2119 18-19 0.09243620418278908 2119 20-21 -1.7038910327157382 2119 22-23 0.18116915968442981 2119 24-25 0.5232651084004942 2119 26-27 -1.637054935526109 2119 28-29 -1.7784650844195937 2119 30-31 -2.642103515534906 2119 32-33 -1.97034181208128 2119 34-35 -3.3542596810978225 2119 36-37 -1.9767593447756013 2119 38-39 -5.105215177939833 2119 40-41 -1.5365211149942866 2119 42-43 -1.226999893867898 2119 44-45 -0.6825007835662191 2119 46-47 0.7070955115948223 2119 48-49 -1.9907341948116581 2119 50-51 -0.06478210909811821 2119 52-53 -0.5904423298613821 2119 54-55 -1.7933227058358838 2119 56-57 -3.8881740112407783 2119 58-59 -1.9116390616695114 2119 60-61 0.1846433304148647 2119 62-63 -1.5142461739664839 2119 64-65 0.4281121755721955 2119 66-67 -0.757709689626104 2119 68-69 -1.618319260315431 2119 70-71 -2.2196523208164223 2119 72-73 -3.175882321059227 2119 74-75 -4.119425865807841 2119 76 -2.78310240115983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 3.0 24 15.0 25 19.0 26 52.0 27 64.0 28 131.0 29 222.0 30 321.0 31 560.0 32 785.0 33 1168.0 34 1594.0 35 2162.0 36 3074.0 37 3233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.69394911586958 24.225919570245466 10.691636200850557 31.38849511303439 2 15.690517048421995 19.15988957696038 42.08759233007536 23.062001044542267 3 15.138401850332015 27.44161754831008 32.32112213683504 25.098858464522866 4 11.20644631798851 21.054987689323283 38.83458927105872 28.903976721629487 5 10.99007684846676 39.18525703200776 37.0588674177423 12.765798701783183 6 24.367678877863163 39.89405357009625 18.204879504588526 17.533388047452064 7 23.785719615011562 34.63403715586063 21.457882563605164 20.122360665522642 8 20.81623517123032 36.59628441393718 22.03984182645676 20.547638588375737 9 20.062672535999404 14.161008729388943 22.121913004551217 43.65440573006044 10-11 21.137058867417743 25.62486010594643 30.243229127807208 22.99485189882862 12-13 22.524807878833098 21.82720286503022 27.445348056405283 28.202641199731403 14-15 20.52152503170932 24.688502574050585 24.02820264119973 30.76176975304036 16-17 22.32336044169216 27.885548011639184 24.580317839289712 25.210773707378948 18-19 22.051033350742372 28.23248526449302 24.28933820786391 25.427143176900696 20-21 21.454152055509958 25.78900246213534 24.30426024024472 28.452585242109972 22-23 21.013952100276057 29.702305454002836 25.882265164515406 23.4014772812057 24-25 22.218906215026486 30.05670372304708 24.565395806908903 23.158994255017536 26-27 20.88711482503917 30.474520629709765 27.15436842497948 21.48399612027158 28-29 19.64112512124151 32.03387301350444 26.449302394986198 21.87569947026785 30-31 21.331045288368276 28.66149369544132 29.060658061627993 20.94680295456241 32-33 21.19674699694098 30.41110199209132 27.54607177497575 20.846079235991944 34-35 19.712004775050364 28.65030217115571 31.40714765351041 20.23054540028352 36-37 19.324031933149293 28.620458106394093 28.50854286353801 23.5469670969186 38-39 21.401924942177125 26.1620532716556 27.69156159068865 24.744460195478624 40-41 19.760501380287995 26.740282026412 30.179810490188764 23.31940610311124 42-43 23.069462060732672 25.236887264045365 30.310378273520854 21.383272401701113 44-45 19.46206073267179 26.639558307841526 28.795791986868615 25.102588972618072 46-47 23.32686711930165 24.882488995001122 28.30336491830187 23.48727896739536 48-49 20.42453182123405 24.266955159292696 29.318063120196967 25.99044989927628 50-51 18.842796388868162 24.990673729761994 30.698351115421918 25.468178765947926 52-53 19.797806461240018 25.449526225471907 33.13064239349399 21.622024919794075 54-55 17.387898231739165 25.386107587853466 31.358651048272772 25.867343132134597 56-57 22.017458777885548 25.083936432142057 28.974856375438335 23.92374841453406 58-59 18.29814220696859 29.553085130194734 29.9410579720958 22.20771469074088 60-61 20.24546743266433 31.645900171603376 26.37096172498694 21.737670670745356 62-63 17.227486383645452 34.13414907110349 25.889726180705814 22.74863836454525 64-65 17.828098186973065 34.52212191300455 24.47959412071924 23.17018577930314 66-67 17.570693128404088 33.98865925539059 25.214504215474147 23.226143400731182 68-69 17.712452436021785 33.50742371110945 24.78176527643065 23.99835857643811 70-71 17.917630381257926 33.4178915168246 24.285607699768708 24.378870402148774 72-73 18.00716257554279 30.414832500186524 25.15854659404611 26.419458330224575 74-75 18.58912183839439 30.922181601134074 24.729538163097814 25.75915839737372 76 17.97358800268597 31.5377154368425 25.136163545474894 25.35253301499664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 4.0 21 6.0 22 4.0 23 6.0 24 11.5 25 13.0 26 19.0 27 42.0 28 66.0 29 73.0 30 93.5 31 119.0 32 142.5 33 161.0 34 210.0 35 314.5 36 499.5 37 629.0 38 763.5 39 954.0 40 982.5 41 983.5 42 1012.0 43 990.5 44 980.0 45 988.5 46 986.0 47 982.0 48 966.0 49 859.5 50 765.0 51 679.5 52 516.0 53 373.0 54 308.0 55 312.0 56 244.0 57 143.0 58 114.0 59 81.5 60 38.5 61 21.0 62 14.0 63 8.5 64 1.5 65 0.5 66 0.5 67 0.0 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.4566141908528 #Duplication Level Percentage of deduplicated Percentage of total 1 70.68986795014193 42.73670073864061 2 16.722201653708503 20.21935387599791 3 6.639516228557324 12.042080131313886 4 2.986548192027644 7.222263672312169 5 1.4562507713192645 4.401999552339029 6 0.5059854374922867 1.8354099828396628 7 0.37023324694557574 1.566813399985078 8 0.17277551524126866 0.835633813325375 9 0.1480932987782303 0.8057897485637544 >10 0.2838454893249414 3.1709318809221814 >50 0.0 0.0 >100 0.024682216463038382 5.1630232037603525 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 399 2.976945459971648 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 293 2.186077743788704 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 47 0.3506677609490413 No Hit CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 43 0.32082369618742074 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 32 0.23875251809296424 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 32 0.23875251809296424 No Hit TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT 29 0.21636946952174885 No Hit CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG 21 0.1566813399985078 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 20 0.14922032380810266 No Hit CTGAACCCTCGTGGAGCCATTCATACAGGTCCCTATTTAAGGAACAAGTG 18 0.1342982914272924 No Hit ACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCT 17 0.1268372752368873 RNA PCR Primer, Index 15 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGTCTT 16 0.11937625904648212 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0074610161904051335 0.0 5 0.0 0.0 0.0 0.0074610161904051335 0.0 6 0.0 0.0 0.0 0.014922032380810267 0.0 7 0.0 0.0 0.0 0.014922032380810267 0.0 8 0.0 0.0 0.0 0.014922032380810267 0.0 9 0.0 0.0 0.0 0.0223830485712154 0.0 10 0.0 0.0 0.0 0.0223830485712154 0.0 11 0.0 0.0 0.0 0.0223830485712154 0.0 12 0.0 0.0 0.0 0.0223830485712154 0.0 13 0.0 0.0 0.0 0.0223830485712154 0.0 14 0.0 0.0 0.0 0.0223830485712154 0.0 15 0.0 0.0 0.0 0.0223830485712154 0.0 16 0.0 0.0 0.0 0.029844064761620534 0.0 17 0.0 0.0 0.0 0.037305080952025664 0.0 18 0.0 0.0 0.0 0.05968812952324107 0.0 19 0.0 0.0 0.0 0.07461016190405133 0.0 20 0.0 0.0 0.0 0.0895321942848616 0.0 21 0.0 0.0 0.0 0.10445422666567186 0.0 22 0.0 0.0 0.0 0.111915242856077 0.0 23 0.0 0.0 0.0 0.11937625904648214 0.0 24 0.0 0.0 0.0 0.12683727523688726 0.0 25 0.0 0.0 0.0 0.1342982914272924 0.0 26 0.0 0.0 0.0 0.1342982914272924 0.0 27 0.0 0.0 0.0 0.1342982914272924 0.0 28 0.0 0.0 0.0 0.14175930761769753 0.0 29 0.0 0.0 0.0 0.16414235618891293 0.0 30 0.0 0.0 0.0 0.19398642095053345 0.0 31 0.0 0.0 0.0 0.2014474371409386 0.0 32 0.0 0.0 0.0 0.223830485712154 0.0 33 0.0 0.0 0.0 0.2536745504737745 0.0 34 0.0 0.0 0.0 0.2984406476162053 0.0 35 0.0 0.0 0.0 0.37305080952025665 0.0 36 0.0 0.0 0.0 0.4924270685667388 0.0 37 0.0 0.0 0.0 0.6416473923748415 0.0 38 0.0 0.0 0.0 0.8803999104678057 0.0 39 0.0 0.0 0.0 1.1564575095127956 0.0 40 0.0 0.0 0.0 1.4996642542714318 0.0 41 0.0 0.0 0.0 1.790643885697232 0.0 42 0.0 0.0 0.0 2.23830485712154 0.0 43 0.0 0.0 0.0 2.6188166828322017 0.0 44 0.0 0.0 0.0 3.044094605685294 0.0 45 0.0 0.0 0.0 3.4917555771096023 0.0 46 0.0 0.0 0.0 3.9244945161531 0.0 47 0.0 0.0 0.0 4.401999552339029 0.0 48 0.0 0.0 0.0 4.961575766619413 0.0 49 0.0 0.0 0.0 5.528612997090204 0.0 50 0.0 0.0 0.0 5.998657017085727 0.0 51 0.0 0.0 0.0 6.513467134223681 0.0 52 0.0 0.0 0.0 6.946206073267179 0.0 53 0.0 0.0 0.0 7.475938222785944 0.0 54 0.0 0.0 0.0 7.960904275162277 0.0 55 0.0 0.0 0.0 8.475714392300231 0.0 56 0.0 0.0 0.0 9.19943296276953 0.0 57 0.0 0.0 0.0 9.684399015145862 0.0 58 0.0 0.0 0.0 10.243975229426248 0.0 59 0.0 0.0 0.0 10.788629411325823 0.0 60 0.0 0.0 0.0 11.348205625606207 0.0 61 0.0 0.0 0.0 11.825710661792137 0.0 62 0.0 0.0 0.0 12.347981795120495 0.0 63 0.0 0.0 0.0 12.758337685592778 0.0 64 0.0 0.0 0.0 13.332835932253973 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTTCT 15 0.002177095 70.00001 68 CTCTGTT 15 0.002177095 70.00001 21 AGTTCTT 20 0.0068037817 52.500004 69 AAGTTAA 170 3.6379788E-12 26.764706 28 AATCTCG 150 7.003109E-10 25.666666 33 CCGTCTT 150 7.003109E-10 25.666666 44 GTCTTCT 150 7.003109E-10 25.666666 46 TCTTCTG 150 7.003109E-10 25.666666 47 CGTCTTC 150 7.003109E-10 25.666666 45 GTTAATC 150 7.003109E-10 25.666666 30 AGTTAAT 150 7.003109E-10 25.666666 29 TTAATCT 165 7.2759576E-11 25.454546 31 ATAAGTT 180 7.2759576E-12 25.277777 26 CTCGTAT 180 7.2759576E-12 25.277777 36 ATCTCGT 155 1.02591E-9 24.838709 34 GAAAAAA 155 1.02591E-9 24.838709 57 TAATCTC 155 1.02591E-9 24.838709 32 GCCGTCT 155 1.02591E-9 24.838709 43 CTTGAAA 155 1.02591E-9 24.838709 54 CTTCTGC 155 1.02591E-9 24.838709 48 >>END_MODULE