FastQCFastQC Report
Wed 25 May 2016
SRR1780727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178645
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCA254.387827E-544.06739814
GCACCAT207.7796873E-444.06739815
CCATCGG207.7904575E-444.05502318
GATGTCC254.4243458E-544.0056125
AATCGTC207.8553305E-443.9809428
CCGAGTG302.5280478E-643.9686232
GTCCCAA207.866184E-443.9686232
TCCCAAC207.866184E-443.9686233
CGGAATC301.2850578E-436.7228320
GGAATCG301.2850578E-436.7228321
GCGGAAT301.287184E-436.7125219
GGATGTC301.2893131E-436.70221724
CGGGATG301.29358E-436.68162522
AATCGGG301.29358E-436.68162523
GTCCTGA301.3000022E-436.65078728
TAGCGGC301.3086062E-436.609747
TCGGGAT250.002329865735.25391821
ATCGGGA250.002329865735.25391820
CTCCAAT250.002336295835.23412712
CCCTGGA250.002336295835.23412713
Produced by FastQC (version 0.11.5)