FastQCFastQC Report
Wed 25 May 2016
SRR1780727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178645
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT100195.608329368300261No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT46802.619720675081866Illumina PCR Primer Index 11 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC15250.8536482969016765No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG10300.5765624562680175No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT4610.25805368188306416Illumina PCR Primer Index 11 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC4580.2563743737580117No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC3980.22278821125696213No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG3230.18080550813065016No Hit
ATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCTGCTT2520.14106188250440818Illumina PCR Primer Index 11 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2110.11811133812869097No Hit
ACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTTCT1930.10803548937837612Illumina PCR Primer Index 11 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA207.8295235E-444.010362
TGTACCA254.421553E-544.0103572
GGGTAAG254.4288718E-543.99803514
CCGTCTT6150.037.91700744
GGGGTGC357.2579423E-637.7126058
ATAAAGA601.9463187E-1036.685571
CTTTTTA301.2970385E-436.6650312
ACTAGCT301.2970385E-436.6650331
CGCCATT601.9645086E-1036.6650317
GACTAGC301.2970385E-436.6650330
GATGTAT250.002347958735.1984324
CTGTACG250.002347958735.1984341
CGACAGC250.002347958735.1984310
CCTGTAC250.002347958735.1984340
ATATAAT250.002347958735.1984312
CTCAGTG250.002347958735.1984334
AGTGTCA250.002347958735.1984337
GCCGTCT6750.034.54660843
TTCAGCC654.620233E-1033.84464644
TTATTCA401.808526E-533.007775
Produced by FastQC (version 0.11.5)