FastQCFastQC Report
Wed 25 May 2016
SRR1780726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38676
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.00220943869.9935356
TTAATGC150.00220943869.9935322
ATGCCAT150.00220943869.9935343
TAATGCA150.00220943869.9935323
GTCCTAC200.006728831752.8369141
CTATGGA200.00690409252.4951484
GAGCTTT200.00690409252.4951489
GGGTCTT200.00690409252.49514828
AGCTTTA200.00690409252.49514810
AGAAGTA200.00690409252.49514848
CTAACTA200.00690409252.4951489
AATGCAA200.00690409252.49514824
TGATTCA200.00690409252.49514857
GAGCCTG200.00690409252.4951489
CCTATGG200.00690409252.4951483
ATGCAAA200.00690409252.49514825
AGTACCT200.00690409252.49514833
GCTTTAA200.00690409252.49514811
TGCAAAC200.00690409252.49514826
TTATTCC450.00425956431.10823626
Produced by FastQC (version 0.11.5)